JPS56124395A - Stabilized coenzyme aqueous solution - Google Patents

Stabilized coenzyme aqueous solution

Info

Publication number
JPS56124395A
JPS56124395A JP1703581A JP1703581A JPS56124395A JP S56124395 A JPS56124395 A JP S56124395A JP 1703581 A JP1703581 A JP 1703581A JP 1703581 A JP1703581 A JP 1703581A JP S56124395 A JPS56124395 A JP S56124395A
Authority
JP
Japan
Prior art keywords
coenzyme
enzyme
substrate
assay
aqueous solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1703581A
Other languages
English (en)
Other versions
JPS6335238B2 (ja
Inventor
Endore Modoroobitsuchi Iwan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JPS56124395A publication Critical patent/JPS56124395A/ja
Publication of JPS6335238B2 publication Critical patent/JPS6335238B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP1703581A 1980-02-13 1981-02-09 Stabilized coenzyme aqueous solution Granted JPS56124395A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/121,225 US4394449A (en) 1980-02-13 1980-02-13 Stabilization of coenzymes in aqueous solution

Publications (2)

Publication Number Publication Date
JPS56124395A true JPS56124395A (en) 1981-09-30
JPS6335238B2 JPS6335238B2 (ja) 1988-07-14

Family

ID=22395341

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1703581A Granted JPS56124395A (en) 1980-02-13 1981-02-09 Stabilized coenzyme aqueous solution

Country Status (6)

Country Link
US (1) US4394449A (ja)
EP (1) EP0034213B1 (ja)
JP (1) JPS56124395A (ja)
AT (1) ATE10208T1 (ja)
CA (1) CA1178875A (ja)
DE (1) DE3069608D1 (ja)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3044454C2 (de) * 1980-11-26 1982-12-09 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierte Enzymzubereitung
US4578265A (en) * 1981-08-13 1986-03-25 Laclede Professional Products, Inc. Di-enzymatic dentifrice
AU574646B2 (en) * 1982-07-19 1988-07-14 Cooperbiomedical Inc. Enzyme assay method
DE3236388A1 (de) * 1982-10-01 1984-04-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur selektiven herstellung reduzierter sauerstoffspezies und hierfuer geeignete reagentien
US4657854A (en) * 1983-05-05 1987-04-14 Ivan Endre Modrovich Assay systems based on magnesium-responsive enzymes
US4622294A (en) * 1985-02-08 1986-11-11 Kung Viola T Liposome immunoassay reagent and method
JPH0673475B2 (ja) * 1985-04-26 1994-09-21 オリエンタル酵母工業株式会社 イソクエン酸脱水素酵素の反応停止法
US5258286A (en) * 1985-07-02 1993-11-02 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction
EP0227073A3 (en) * 1985-12-23 1989-03-22 F. Hoffmann-La Roche Ag Method of determining a co-enzyme
US4897346A (en) * 1986-07-15 1990-01-30 Beckman Instruments, Inc. Stabilized liquid enzyme composition for glucose determination
US4940659A (en) * 1987-02-20 1990-07-10 Monoclonetics International, Inc. Screening extra-cellular body fluids for superoxide dismutase (SOD-1) for determining fetal trisomy 21 down syndrome
US5409814A (en) * 1987-04-10 1995-04-25 Boehringer Mannheim Gmbh Determination of ions in fluids
GB8710882D0 (en) * 1987-05-08 1987-06-10 Health Lab Service Board Estimation of reduced pyridine nucleotides
ATE135746T1 (de) * 1989-08-28 1996-04-15 Hoffmann La Roche Enzymatisches verfahren zur bestimmung der analyt-konzentration
EP0463755A1 (en) * 1990-06-27 1992-01-02 INSTRUMENTATION LABORATORY S.p.A. Stable aqueous NADH reagent and kit
US5116728A (en) * 1990-07-20 1992-05-26 Em Diagnostic Systems, Incorporated Reagents for co2 detection
CA2111168A1 (en) * 1992-12-21 1994-06-22 Shing F. Kwan Stable single liquid reagent for the determination of carbon dioxide in serum
AU682882B2 (en) * 1993-09-17 1997-10-23 Thermo Trace Ltd. Reagent
EP0719345B1 (en) * 1993-09-17 2000-09-27 Trace Scientific Limited Reagent for enzymatic determination of serum bicarbonate levels
AUPN200695A0 (en) * 1995-03-28 1995-04-27 Trace Scientific Pty Ltd Transaminase reagent
AU710552B2 (en) * 1995-03-28 1999-09-23 Thermo Trace Ltd. Reagent stabilized using coenzyme reduction system
ES2306453T3 (es) * 1995-12-27 2008-11-01 Asahi Kasei Pharma Corporation Metodo para ensayar una muestra biologica.
JP3674015B2 (ja) * 1996-10-02 2005-07-20 日東紡績株式会社 尿素窒素の測定方法およびその測定用キット
US5801006A (en) * 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
JP2005502363A (ja) * 2001-09-11 2005-01-27 メルク　パテント　ゲゼルシャフト　ミット　ベシュレンクテル　ハフトング 酵素アッセイのためのラテラルフローテストフォーマット
US6703216B2 (en) 2002-03-14 2004-03-09 The Regents Of The University Of California Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB)
AU2003261614A1 (en) * 2003-09-05 2005-03-29 Fenghui (Shanghai) Medical Science And Tech. Co., Ltd The agent for assaying analyte of patient by enzyme
ITMI20060629A1 (it) * 2006-03-31 2007-10-01 Daniele Giovannone Composizioni solide orali a base di s-adenosilmetionina e processo per il loro ottenimento
CN104458617A (zh) * 2014-12-05 2015-03-25 重庆中元生物技术有限公司 一种α-羟丁酸脱氢酶检测试剂盒及其制备方法
JP2022518594A (ja) * 2019-01-31 2022-03-15 エフ．ホフマン－ラ ロシュ アーゲー アンモニア検出アッセイにおけるｎａｄｐｈ又はｎａｄｈの安定化

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5280886A (en) * 1975-12-27 1977-07-06 Chugai Pharmaceutical Co Ltd Reagent composition for measurement
JPS52134087A (en) * 1976-03-17 1977-11-09 Modrovich Ivan Endre Stabilized liquid coenzyme composition
JPS53107486A (en) * 1977-02-28 1978-09-19 Wako Pure Chem Ind Ltd Stabilized nadh aqueous solution
JPS5519095A (en) * 1978-07-18 1980-02-09 Boehringer Mannheim Gmbh Glycerine determining method and reagent

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3413198A (en) * 1966-06-30 1968-11-26 Calbiochem Reagents and method for assaying biological samples
US3776900A (en) * 1971-04-26 1973-12-04 Searle & Co Stabilization of reduced coenzymes
FR2217296A1 (en) * 1972-10-10 1974-09-06 Inst Francais Du Petrole Organic cpds. prepn. by co-enzymatic reduction - with continuous regeneration of the oxidised coenzyme with a reducing agent
AT339505B (de) * 1974-03-14 1977-10-25 Boehringer Mannheim Gmbh Enzymatisches analysenverfahren
NL7603588A (nl) 1975-04-21 1976-10-25 Hoffmann La Roche Gestabiliseerde coenzymoplossing.
FR2344570A1 (fr) * 1976-03-17 1977-10-14 Modrovich Ivan Procede de stabilisation d'un coenzyme labile
US4277562A (en) * 1976-09-13 1981-07-07 Modrovich Ivan Endre Stabilized liquid enzyme and coenzyme compositions
US4189536A (en) * 1977-11-09 1980-02-19 Hycel, Inc. Reagent systems, enzymatic assays and compositions therefor
CA1187388A (en) * 1978-09-20 1985-05-21 American Monitor Corporation Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays
US4218536A (en) * 1978-10-03 1980-08-19 Jonas Maurukas Process-stable co-enzyme NAD solution
US4247633A (en) * 1979-04-05 1981-01-27 Pabst Brewing Company Reagent for colorimetric determination of creative phosphokinase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5280886A (en) * 1975-12-27 1977-07-06 Chugai Pharmaceutical Co Ltd Reagent composition for measurement
JPS52134087A (en) * 1976-03-17 1977-11-09 Modrovich Ivan Endre Stabilized liquid coenzyme composition
JPS53107486A (en) * 1977-02-28 1978-09-19 Wako Pure Chem Ind Ltd Stabilized nadh aqueous solution
JPS5519095A (en) * 1978-07-18 1980-02-09 Boehringer Mannheim Gmbh Glycerine determining method and reagent

Also Published As

Publication number Publication date
JPS6335238B2 (ja) 1988-07-14
EP0034213A1 (en) 1981-08-26
ATE10208T1 (de) 1984-11-15
US4394449A (en) 1983-07-19
DE3069608D1 (en) 1984-12-13
CA1178875A (en) 1984-12-04
EP0034213B1 (en) 1984-11-07

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