DE2334424A1 - Verfahren und vorrichtung zum differenzierenden zaehlen von blutkoerperchen, insbesondere von weissen blutkoerperchen - Google Patents
Verfahren und vorrichtung zum differenzierenden zaehlen von blutkoerperchen, insbesondere von weissen blutkoerperchenInfo
- Publication number
- DE2334424A1 DE2334424A1 DE19732334424 DE2334424A DE2334424A1 DE 2334424 A1 DE2334424 A1 DE 2334424A1 DE 19732334424 DE19732334424 DE 19732334424 DE 2334424 A DE2334424 A DE 2334424A DE 2334424 A1 DE2334424 A1 DE 2334424A1
- Authority
- DE
- Germany
- Prior art keywords
- particles
- circle
- blood cells
- cells
- coordinates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 20
- 210000000265 leukocyte Anatomy 0.000 title claims description 18
- 210000000601 blood cell Anatomy 0.000 title claims description 16
- 239000002245 particle Substances 0.000 claims description 21
- 238000001962 electrophoresis Methods 0.000 claims description 17
- 230000015654 memory Effects 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 8
- 230000005684 electric field Effects 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 230000005012 migration Effects 0.000 claims description 3
- 238000013508 migration Methods 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 61
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 10
- 230000033001 locomotion Effects 0.000 description 9
- 238000005259 measurement Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 101800000263 Acidic protein Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000781 anti-lymphocytic effect Effects 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 201000006747 infectious mononucleosis Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH995372A CH560039A5 (enrdf_load_html_response) | 1972-07-03 | 1972-07-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2334424A1 true DE2334424A1 (de) | 1974-01-24 |
Family
ID=4357490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19732334424 Pending DE2334424A1 (de) | 1972-07-03 | 1973-07-03 | Verfahren und vorrichtung zum differenzierenden zaehlen von blutkoerperchen, insbesondere von weissen blutkoerperchen |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS4943481A (enrdf_load_html_response) |
| CH (1) | CH560039A5 (enrdf_load_html_response) |
| DE (1) | DE2334424A1 (enrdf_load_html_response) |
| FR (1) | FR2236472A1 (enrdf_load_html_response) |
| NL (1) | NL7309208A (enrdf_load_html_response) |
-
1972
- 1972-07-03 CH CH995372A patent/CH560039A5/xx not_active IP Right Cessation
-
1973
- 1973-07-02 NL NL7309208A patent/NL7309208A/xx unknown
- 1973-07-02 FR FR7324197A patent/FR2236472A1/fr not_active Withdrawn
- 1973-07-03 JP JP7445973A patent/JPS4943481A/ja active Pending
- 1973-07-03 DE DE19732334424 patent/DE2334424A1/de active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JPS4943481A (enrdf_load_html_response) | 1974-04-24 |
| NL7309208A (enrdf_load_html_response) | 1974-01-07 |
| CH560039A5 (enrdf_load_html_response) | 1975-03-27 |
| FR2236472A1 (en) | 1975-02-07 |
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