DE2104810A1 - Verfahren zur Stabilisierung von Enzymen - Google Patents

Verfahren zur Stabilisierung von Enzymen

Info

Publication number
DE2104810A1
DE2104810A1 DE19712104810 DE2104810A DE2104810A1 DE 2104810 A1 DE2104810 A1 DE 2104810A1 DE 19712104810 DE19712104810 DE 19712104810 DE 2104810 A DE2104810 A DE 2104810A DE 2104810 A1 DE2104810 A1 DE 2104810A1
Authority
DE
Germany
Prior art keywords
enzyme
parts
polymer
carrier
bound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE19712104810
Other languages
German (de)
English (en)
Inventor
Peter Salvatore Stam ford Conn Forgione (V St A)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of DE2104810A1 publication Critical patent/DE2104810A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C12N11/12—Cellulose or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE19712104810 1970-02-02 1971-02-02 Verfahren zur Stabilisierung von Enzymen Pending DE2104810A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US808970A 1970-02-02 1970-02-02
US5733970A 1970-07-22 1970-07-22
US5734070A 1970-07-22 1970-07-22

Publications (1)

Publication Number Publication Date
DE2104810A1 true DE2104810A1 (de) 1971-08-19

Family

ID=27358510

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19712104810 Pending DE2104810A1 (de) 1970-02-02 1971-02-02 Verfahren zur Stabilisierung von Enzymen

Country Status (5)

Country Link
CA (1) CA954461A (cs)
DE (1) DE2104810A1 (cs)
FR (1) FR2078092A5 (cs)
GB (1) GB1321352A (cs)
NL (1) NL7101058A (cs)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3705687A1 (de) * 1987-02-23 1988-09-01 Boehringer Mannheim Gmbh Polyurethanmodifizierte enzyme
EP0360452A3 (en) * 1988-09-06 1992-01-08 Eastman Kodak Company Latex particles in analytical reagents, elements and methods

Also Published As

Publication number Publication date
CA954461A (en) 1974-09-10
GB1321352A (en) 1973-06-27
FR2078092A5 (cs) 1971-11-05
NL7101058A (cs) 1971-08-04

Similar Documents

Publication Publication Date Title
EP0075815B1 (de) Wasserunlösliches Proteinmaterial, dessen Herstellung und Verwendung
DE2915135C2 (cs)
EP0097898B1 (de) Makroporöse, hydrophile Träger für Enzyme
DE2061371C3 (de) Verfahren zur Herstellung eines immobilisierten Glucoseisomeraseenzymkomplexes und dessen Verwendung
DE2407961C3 (de) Enzymatisch aktive Membran, Verfahren zu ihrer Herstellung und ihre Verwendung
DE2721829A1 (de) Verfahren zur herstellung eines hydrophilen komplexen gels zur immobilisierung mikrobieller zellen
FR2646777A1 (fr) Procede de preparation d'un produit particulaire antimicrobien, produit antimicrobien obtenu et applications
DE1908290A1 (de) Zur Fixierung von Proteinen verwendbares Mischpolymerisat
DE2633259B2 (de) Verfahren zum Unbeweglichmachen von Enzymen
US4978619A (en) Enzyme immobilization by entrapment in a polymer gel matrix
DE3801053C2 (cs)
US3766013A (en) Preparation of water-insoluble carrier bound enzymes
DE2353102A1 (de) Faserhaltige matten und folien, die in ihren zwischenraeumen immobilisierte enzyme eingeschlossen enthalten
DE69705023T2 (de) Enzymatische gelierung von polymermaterialien
DE2336829A1 (de) Wasserunloesliches enzympraeparat, verfahren zu seiner herstellung und seine verwendung
Mårtensson et al. Covalent coupling of pullulanase to an acrylic copolymer using a water soluble carbodi‐imide
Garde et al. Electron microscopic evidence of an immobilized living cell system
EP0097281B1 (de) Verfahren zur Herstellung eines unlöslichen Biokatalysators
DE2104810A1 (de) Verfahren zur Stabilisierung von Enzymen
DE3315201A1 (de) Verfahren zum fixieren enzymatisch aktiver materialien
DE2410693A1 (de) Verfahren zum immobilisieren von enzymen
DE2707024A1 (de) Verfahren zum fixieren eines proteins auf einem traeger
US3770588A (en) Storage stabilization of carrier bound enzymes
US3734831A (en) Production of cellulase
DE2413694C3 (cs)