DE202004011490U1 - Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor - Google Patents

Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor Download PDF

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Publication number
DE202004011490U1
DE202004011490U1 DE200420011490 DE202004011490U DE202004011490U1 DE 202004011490 U1 DE202004011490 U1 DE 202004011490U1 DE 200420011490 DE200420011490 DE 200420011490 DE 202004011490 U DE202004011490 U DE 202004011490U DE 202004011490 U1 DE202004011490 U1 DE 202004011490U1
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DE
Germany
Prior art keywords
adapter
transport
sampling
phase microextraction
threaded ring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE200420011490
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German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BLEI INST GmbH
BLEI-INSTITUT GmbH
Original Assignee
BLEI INST GmbH
BLEI-INSTITUT GmbH
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Filing date
Publication date
Application filed by BLEI INST GmbH, BLEI-INSTITUT GmbH filed Critical BLEI INST GmbH
Priority to DE200420011490 priority Critical patent/DE202004011490U1/en
Publication of DE202004011490U1 publication Critical patent/DE202004011490U1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/009—Extraction

Abstract

Solid phase microextraction and transport device comprises a stainless steel tube, into which a threaded ring screws. An adapter screws into this which can be used to give an airtight connection e.g. to a bioreactor.

Description

Probenahmesysteme wie das Festphasenmikroextraktionssystem (SPME – Solid Phase MicroExtraction) weisen nach üblicher Bauart einen manuellen oder einen automatischen Probenhalter für die automatisierte Auswertung mit HPLC oder GC auf. Die Probehalter zeigen eine universelle Anwendbarkeit für die Probenahme und sind nicht auf eine spezifische Methode der Probenahme begrenzt bzw. angepasst.sampling systems like the solid phase micro extraction system (SPME - Solid Phase MicroExtraction) show according to usual Design a manual or an automatic sample holder for the automated Evaluation with HPLC or GC. The sample holder shows a universal Applicability for the sampling and are not based on a specific method of sampling limited or adjusted.

Um geschlossene Systeme wie z.B. Bioreaktoren zu beproben sind diese Probehalter unzweckmäßig. Denn beim Beproben der Systeme kann ein luftdichter und steriler Abschluss nicht gewährleistet werden.Around closed systems such as These are bioreactors to be sampled Inappropriate test holder. Because when sampling the systems, an airtight and sterile seal can be used cannot be guaranteed.

Der im Schutzanspruch 1 angegebenen Erfindung liegt das Problem zugrunde, eine abgeschlossene, sterile Verbindung zu schaffen, die eine Probenahme im Bioreaktor oder in einem abgeschlossenen System, z.B. mittels SPME, ermöglicht.The The invention specified in claim 1 is based on the problem to create a sealed, sterile connection that is a sampling in the bioreactor or in a closed system, e.g. by means of SPME, enables.

Dieses Problem wird mit den im Schutzanspruch 1 aufgeführten Merkmalen gelöst.This Problem is solved with the features listed in protection claim 1.

Mit dieser Erfindung kann ein sterile Probenahme in Bioreaktoren oder anderen derart abgeschlossenen Systemen durch das Adaptionssystem erreicht werden.With This invention can be used in sterile sampling in bioreactors or other systems closed in this way achieved by the adaptation system become.

Im weiteren liegt das Problem des Transports der Probenahmeeinheit zum Probenahmeort zugrunde.in the there is also the problem of transporting the sampling unit to the sampling location.

Dieses Problem wird mit den im Schutzanspruch 2 aufgeführten Merkmalen gelöst.This Problem is solved with the features listed in protection claim 2.

Das Transportsystem ermöglicht die Lagerung der Faser in einer sterilen Atmosphäre bis zu 48h. Dadurch wird ein einfacher und unkomplizierter Transport der Faser zu verschiedenen Probenahmeorten möglich.The Transport system enables storage of the fiber in a sterile atmosphere for up to 48 hours. This will a simple and uncomplicated transport of the fiber to different Sampling locations possible.

Durch die technische Zeichnung (Draufsicht und Seitenansicht von rechts) werden die Bestandteile des Transportsystems dargestellt. Ein Ausführungsbeispiel wird anhand der 5 und 6 erläutert.The technical drawing (top view and side view from the right) shows the components of the transport system. An embodiment is based on the 5 and 6 explained.

Es zeigen:It demonstrate:

5 das Probenahmesystem (hier SPME) in einer sterilen Atmosphäre für Lagerung und Transport 5 the sampling system (here SPME) in a sterile atmosphere for storage and transport

6 die Adaptereinheit verschraubt am Biorektor zur sterilen Probenahme In den Figuren ist die Transporthülse und der Bioreaktor mit Gewindering 1 und Adapteraufsatz 2 dargestellt. Dieses Adaptersystem schließt die Transporthülse und den Bioreaktor luftdicht ab. 6 the adapter unit is screwed to the bioreactor for sterile sampling. The figures show the transport sleeve and the bioreactor with threaded ring 1 and adapter attachment 2. This adapter system seals the transport sleeve and the bioreactor airtight.

Anwendungapplication

Vor jeder Verwendung des Transportsystems ist die Sterilisation im Trockenschrank bei 150°C für einen Zeitraum von mind einer Stunde notwendig. Dabei wird der Blindstopfen in das Innengewinde des Adapteraufsatzes geschraubt, welcher auf die Zylinderhülse aufgesetzt ist. Häufig können Kunststoffteile des Probenahmesystems (z.B. Probehalter der SPME) einer Temperatur von 150°C nicht ausgesetzt werden. So kann z.B. die Sterilisation der SPME-Faser in der Injektoreinheit des Gaschromatographen erfolgen. In Vorbereitung auf den Transport wird unter sterilen Bedingungen, z.B. durch Beflammung, der Blindstopfen mit dem Gewindering und der Probenahmeeinheit ausgetauscht.In front Every use of the transport system is sterilization in the drying cabinet at 150 ° C for one A period of at least one hour is necessary. The blind plug is used screwed into the internal thread of the adapter attachment, which on the cylinder sleeve is put on. Frequently can Plastic parts of the sampling system (e.g. sample holder from SPME) a temperature of 150 ° C not be exposed. For example, the sterilization of the SPME fiber in the injector unit of the gas chromatograph. In preparation transport is carried out under sterile conditions, e.g. by flame, the Blind plug replaced with the threaded ring and the sampling unit.

Erläuterungexplanation

1: Edelstahlhülse 1 : Stainless steel sleeve

2: Adapterteil mit durchgehender Lochbohrung 2 : Adapter part with through hole

3: Gewindering als Verbindung der Festphasenmikroextraktionsfaser mit dem Adapterteil (2) 3 : Threaded ring as connection of the solid phase micro extraction fiber with the adapter part ( 2 )

4: Blindstopfen für den Adapter 4 : Blind plug for the adapter

5: Festphasenmikroextraktionsfaser verschraubt mit dem Adaptersystem und der Edelstahlhülse 5 : Solid phase micro extraction fiber screwed to the adapter system and the stainless steel sleeve

6: Festphasenmikroextraktionsfaser verschraubt mit dem Adaptersystem und dem Fermenter 6 : Solid phase micro extraction fiber screwed to the adapter system and the fermenter

Claims (3)

Ein Transport- und Probenahmesystem für die Festphasenmikroextraktion mit einem Adaptersystem welches eine sterile Probenahme in Bioreaktoren oder derart abgeschlossenen Systemen ermöglicht dadurch gekennzeichnet, dass der Gewindering (3) die aktive Probenahmeeinheit luftdicht mit dem Adapteraufsatz verbindet und der Adapteraufsatz (2) luftdicht am Bioreaktor abschließt.A transport and sampling system for solid-phase microextraction with an adapter system which enables sterile sampling in bioreactors or such closed systems, characterized in that the threaded ring ( 3 ) connects the active sampling unit airtight to the adapter attachment and the adapter attachment ( 2 ) hermetically seals at the bioreactor. Ein Transport- und Probenahmesystem für die Festphasenmikroextraktion mit einem Adaptersystem nach Anspruch 1 dadurch gekennzeichnet, dass es aus 3 Komponenten besteht (Gewindering, Adapteraufsatz und Blindstopfen) und über das Normgewinde DN 19 (a) mit Bio- oder anderen Reaktoren kompatibel ist. Weiterhin zeichnet es sich durch zwei verschiedene Bohrungsdurchmesser in der Mitte längs durch den Adapteraufsatz aus. Die Bohrung 1 (b) im Adapteraufsatz durchdringt den Adapter in Längsrichtung nicht vollständig. Durch die Bohrung 2 (c) (max. 2mm) entsteht ein Kanal vollständig durch den Adapter. Damit wird eine nur minimale Öffnung der Transporthülse zum Einführen der Probenahmeeinheit erzeugt.A transport and sampling system for solid phase microextraction with an adapter system according to claim 1, characterized in that it consists of 3 components (threaded ring, adapter attachment and blind plug) and is compatible with bio or other reactors via the standard thread DN 19 (a). It also features two different hole diameters in the middle lengthwise through the adapter attachment. The hole 1 (b) in the adapter attachment does not fully penetrate the adapter in the longitudinal direction. Through the hole 2 (c) (max. 2mm) a channel is created completely through the adapter. This creates only a minimal opening of the transport sleeve for inserting the sampling unit. Ein Transport- und Probenahmesystem für die Festphasenmikroextraktion nach Anspruch 1 dadurch gekennzeichnet, dass der Adapter verschraubt mit der Transporthülse durch Hitzesterilisation eine sterile Atmosphäre zum Transport und zur Lagerung des Probenahmesystems bietet (5).A transport and sampling system for solid-phase microextraction according to claim 1, characterized in that the adapter screwed to the transport sleeve by heat sterilization offers a sterile atmosphere for transport and storage of the sampling system ( 5 ).
DE200420011490 2004-07-20 2004-07-20 Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor Expired - Lifetime DE202004011490U1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE200420011490 DE202004011490U1 (en) 2004-07-20 2004-07-20 Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE200420011490 DE202004011490U1 (en) 2004-07-20 2004-07-20 Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor

Publications (1)

Publication Number Publication Date
DE202004011490U1 true DE202004011490U1 (en) 2004-12-02

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DE200420011490 Expired - Lifetime DE202004011490U1 (en) 2004-07-20 2004-07-20 Solid phase microextraction and transport device comprising a stainless steel tube, into which threaded ring screws, and an adapter screwing into this which can be used to give airtight connection e.g. to bioreactor

Country Status (1)

Country Link
DE (1) DE202004011490U1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107456777A (en) * 2017-08-07 2017-12-12 胡国良 Catch tray

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107456777A (en) * 2017-08-07 2017-12-12 胡国良 Catch tray
CN107456777B (en) * 2017-08-07 2020-01-14 胡国良 Collecting tray

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R207 Utility model specification

Effective date: 20050105

R156 Lapse of ip right after 3 years

Effective date: 20080201