DE1467791A1 - Method of excretion, particularly of fetal organs - Google Patents

Description

Process for the elimination of particular rötalorganen The present invention relates to a Ausscheidun4sverfahren that certain animal at rätal- or Nichtfätalorganen and in human rätalorganen such. H. in the case of plasenta, can be used to obtain useful extracts for pharmaceutical compositions .

After separation and after crushing of fresh, frozen with any method or lyophilislerten organs or tissues provides the Inventions "9ige procedure followed in which these organs or tissue three successive precipitations are subjected, respectively in a neutral or near neutral milieu in an alkali and an acid that preserves separated out the Ausaoheidungsrtiokstände gained in the three excretions that you never finally together there and the final product or Bndauescheidung by centrifugation and riltern and possibly purified by lyophilization. this jeweile the three Aueecheidungevorgänge. take place in a neutral or almost neutral, acidic and basic environment, the separation means consist of distilled water or a buffer solution for the first separation process, of distilled water, e.g. alkaline with soda, potash or ammonia for the second separation process and $ . B. by Bssigeä, ure, phosphoric acid or hydrochloric acid acidified, distilled water for the third elimination process, whereby the three elimination must take place at pH values of 6.5 to 7.518 and 9 and below. In the various processes , the weight of the water is one to three times the weight of the organs being treated.

The properties of the Zztrakte finally gained comparable course change with the bodies that have been subject to elimination. So can be taken including fresh or frozen and any lyopholieierte Bötalorgane which deal directly from the fetus of animals, in or just the endochondral? Hurry to be treated by Fötalknoehen of mammals. The invention is veretändlioh on the basis of the following description of four possible examples for the preparation of extracts according to the inventive method.

B e i sp ie 1 i Preparation of an Extract from Petal Organs 5 kg of fetal organs are removed from the 1 to 6-month-old fetus of mammals, comminuted and frozen. These organs are dissolved in 7 liters of distilled water, and the whole is left to itself for 12 hours at ambient temperature , stirring from time to time . Then a separation is carried out by centrifugation and then the residues floating on the surface are preserved at +40 ° .

The sediment is immediately dissolved in 5 liters of distilled water ; aan brings the pH of the solution to 8 by adding sodium hydroxide solution; mm, the solution stirred for 4 hours at + 40 0 long rest; it is centrifuged for the third time and the residues floating on the surface are preserved at + 40 ° C. You have three swims on the surface of these wines. get a lot of residues, which you put together. Through this a solution is obtained whose pX value is between 6.8 and 7.2. This solution is centrifuged at + 40 ° C filtered, and you get the desired I x tract, which is finally still ITophilized. The three eliminations could also be at. other pH can be performed than with the ` Values. The distilled water at the first separation fertilization could also be achieved by a buffer solution with a pH Value ton 695 to 7v59 s. !!. a phosphate buffer solution, be replaced. In the second elimination, the Formation of the lye other than based on latron- be carried out, see H. by potassium or ammonia and at the third excretion, the acid could instead by Ib eigeäure s. ß. by phosphoric acid, salmon or Sulfuric acid are formed. D eisp i e 1 2 lubersium sinei extract from lötalknoahen 245 grams of guohondral parts of long, frozen killing cries a 1 am 3 month old yew fruit are chopped up and dissolved in 600 cubic meters of distilled water. This solution is allowed to rest for 12 hours 40 C at +. It is centrifuged and the residues floating on the surface are preserved at + 40 ° C. The sediment is dissolved in 500,033 distilled water , and the solution is brought to a pH value of 8 by adding sodium hydroxide solution. The solution is leaving for 2 hours at + 40 C himself and stirred from time to time to.

Then it is centrifuged and the residues on the surface are preserved at + 40 ° C.

The sediment is dissolved in 5oo cm3 of distilled water and the p8 of the solution by the addition of Besigsäure to 3.8. The solution is left to itself at + 40 ° C. for 2 hours and is stirred from time to time . It sentritugiett and preserved at the schwim- Menden on the surface residues + 40 C.

The three residues obtained been combined, and a solution is obtained whose pH is in the vicinity of the neutral value. This secretion is centrifuged, then filtered and the clear, final solution is lyophilized. Thus the desired extract with a whitish- pink- colored appearance has been obtained, which is soluble in distilled water, but insoluble in normal organic solvents .

H EISP 1 e 1 3 Preparation of a Bartraktes on the base parallax of Plazentss of säu $ etferen, animals Säue6-of aötallebern of. of lötalailzen of mammals and of aenchlicen placentas.

2 kg placenta of preventing or sheep, 2 kg Liver of cattle or sheep, 1kg Pötalails of binders, which are 1 taken from 9 months old body fruits advertising, crushed the frozen and then placed in 7 liters DES tilli Erten water in solution . The solution is überläBt for 12 hours at Ungebungsteaperatur themselves and stirred from time to time to. The residues floating on the surface are centrifuged and preserved at + 40 ° C.

The sediment is dissolved in 5 liters of distilled water , the pH of the solution being brought to 8 using sodium hydroxide. Ran lets the solution rest for 4 hours at + 40 ° C., sentritutes and preserves the residues floating on the surface at + 40 ° C.

The pellet is redissolved in 5 liters of distilled water and the pH value of the solution by the addition of Besigsäuren to 3.8. Kan leaves this solution to rest for 3 hours at + 40 C, centrifuges and preserves the residues floating on the surface at + 4 ° C.

The three residues obtained in this way are combined and a mixture is obtained with a pH of 698 to 7.2. This mixture is centrifuged and filtered at + 40 ° C.

In parallel with this first preparation , 500 6r crushed and frozen human placenta are treated in 800 OB3 distilled water. The solution is left to rest for 12 hours, then it is centrifuged and the residues floating on the surface are preserved at + 40 °. The sediment is redissolved in 500 cm3 of distilled water . Bringing the pH of the solution by addition of caustic soda solution to 8, the solution is allowed to rest for 4 hours, centrifuged, and the con- floating on the surface residues served at + 40 C.

The pellet is redissolved in 5oo em3 distilled water and the pH value of the solution by the addition of Issig acid to 3.8. Irian lets the solution rest for three hours at + 40 C , centrifuges and preserves the residues floating on the surface at + 40 C.

The three residues obtained in this way are combined, and a mixture is obtained, the pH of which is between 6.8 and 7.2. The mixture is filtered.

Thus won a solution supporter, which is mixed with the solution from the elimination of Pötalorgane of mammals. These two solutions are held together for 4 hours at 40 C +, then centrifuged, filters trated and lyophilized. B e 1 spat 1 4 Z_ubereitunz a Utraktes on the base Lama ton Säuaetiersehesa 25o gr tendons of adult cattle, sheep, or from the Leibestrucht of cattle or sheep crushed and dissolved in 3oo cm3 of distilled water, the solution is allowed for 12 hours at + 40 0 rest. The residues floating on the surface are centrifuged and preserved at + 40 °.

The sediment is added again in 3oo cm3 distilled water ser in solution, bringing the pH of the solution by addition of sodium hydroxide solution. 8 The solution is leaving for 2 hours at + 40 0 itself and stirred from time to time $ u. Fan centrifuges and preserves the residues floating on the surface. The residues obtained from the first and second elimination are mixed. The pH of the mixture will be $. B. brought to a value between 6.8 and?, 2 by means of Eseigsärre. The extract obtained is pink or more or less colorless. $ s is releasable in distilled water Lich, but in common organic solvents it is insoluble. He can z. H. find use in the pharmaceutical industry.

In the four examples described , the four precipitations could also be carried out at different pH values than the specified values. The distilled water during the first excretion could also be replaced by a buffer solution with a pH value of 6.5 to 7.5, for example a phosphate buffer solution , or by a hypertonic solution . In the case of the second separation, the formation of the lye could be carried out differently than on the basis of iatron, a. For example, by Kaliur or ammonia and in the third shedding could be the acid rather than by Besigsäure a. B. by phosphoric acid, hydrochloric or sulfuric acid .

Claims (1)

Claims: A process for the excretion in particular Pötalorganen, characterized in that separate, comminuted and possibly frozen organs subjected to three successive precipitation processes, in each case in neutral or near neutral, basic and acidic Kilieu, DAB to the Ausscheidungeprodukte gained in these three precipitation processes first concerted separately, they finally combined and that the end product or the end extract is purified by centrifugation, filtering and possibly hyophilization . 2. The method of claim i, characterized in that DA8 is used for the first @ excretion as clearing agent of distilled water or a buffer solution. 3. The method according to claim 1, characterized in that distilled water is used as the elimination agent for the second elimination, the $. B. has been made basic by baking soda, zinc or ammonia. 4. The method according to claim 1, characterized in that that for the third elimination as an elimination distilled water is used, which s. B. by acetic acid, phosphoric acid, hydrochloric or sulfuric , acid has been made acidic. 5. The method as claimed in claim 1, characterized in that da8 the three excretion processes at a pH of 6.5 to 7.5, 8 to 9 and 3 to 5, respectively will. 6. The method according to claim 1 and 5, characterized in that that the weight of the distilled water each time Divorce 1 to 3 times the weight of the treated Or @ aane has. ?. Method according to claims 1 and 2, characterized in that that during the first excretion the organs are distilled lated water in gives that aua the solution during Rest for a few hours at the bridge temperature lUt, da8 asn the LU®w M cinnamon and that mm the on the upper .. fläo "aohwimaenden $ üeketände preserved at + 40 0. B. The method according to claim 1, 3 and 7, characterized in that the sediment from the previous centrifugation ih distilled water is dissolved in the second separation, that one night a lye from the solution , that the solution is a few hours at + allowed to rest 40 C that they are centrifuged and in that the surface at the top floating Rüoketände at + 40 konseriiert C. 9. The method according to claim 1, 4, 7 and 8, characterized in that during the third elimination, the sediment obtained from the second centrifugation is dissolved in distilled water, that the solution is converted into acid, that it is for a few hours Let it rest at + 40 ° C, entrifug it and preserve the residues floating on the surface at + 40 ° C.