DE112015003261A5 - Gentechnisch veränderte Hefe mit verbessertem Glycerol-Katabolismus - Google Patents

Gentechnisch veränderte Hefe mit verbessertem Glycerol-Katabolismus Download PDF

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Publication number
DE112015003261A5
DE112015003261A5 DE112015003261.4T DE112015003261T DE112015003261A5 DE 112015003261 A5 DE112015003261 A5 DE 112015003261A5 DE 112015003261 T DE112015003261 T DE 112015003261T DE 112015003261 A5 DE112015003261 A5 DE 112015003261A5
Authority
DE
Germany
Prior art keywords
genetically modified
modified yeast
improved glycerol
glycerol catabolism
catabolism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE112015003261.4T
Other languages
English (en)
Inventor
Elke Nevoigt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Constructor University Bremen Ggmbh De
Original Assignee
Jacobs University gGmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102014109858.7A external-priority patent/DE102014109858A1/de
Application filed by Jacobs University gGmbH filed Critical Jacobs University gGmbH
Publication of DE112015003261A5 publication Critical patent/DE112015003261A5/de
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01029—Glycerone kinase (2.7.1.29), i.e. dihydroxyacetone kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0103—Glycerol kinase (2.7.1.30)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
DE112015003261.4T 2014-07-14 2015-07-10 Gentechnisch veränderte Hefe mit verbessertem Glycerol-Katabolismus Pending DE112015003261A5 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102014109858.7A DE102014109858A1 (de) 2014-07-14 2014-07-14 Gentechnisch veränderte Hefe mit verbessertem Glycerol-Katabolismus
DE102014109858.7 2014-07-14
EP14192729 2014-11-11
EP14192729.3 2014-11-11
PCT/EP2015/065839 WO2016008819A1 (de) 2014-07-14 2015-07-10 Gentechnisch veränderte hefe mit verbessertem glycerol-katabolismus

Publications (1)

Publication Number Publication Date
DE112015003261A5 true DE112015003261A5 (de) 2017-04-27

Family

ID=53762138

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112015003261.4T Pending DE112015003261A5 (de) 2014-07-14 2015-07-10 Gentechnisch veränderte Hefe mit verbessertem Glycerol-Katabolismus

Country Status (3)

Country Link
EP (1) EP3169768B1 (de)
DE (1) DE112015003261A5 (de)
WO (1) WO2016008819A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102016115425A1 (de) 2016-08-19 2018-02-22 Jacobs University Bremen Ggmbh Gentechnisch veränderte Hefe zur Fermentation von Glycerol
EP3559220A1 (de) * 2016-12-23 2019-10-30 DSM IP Assets B.V. Verbesserte herstellung von glycerinfreiem ethanol
WO2018127572A1 (en) * 2017-01-05 2018-07-12 Danmarks Tekniske Universitet A method for improved glycerol utilization in yeast
CN109370969B (zh) * 2018-11-12 2020-09-04 江南大学 一种重组克雷伯氏杆菌在制备1，3-丙二醇中的应用
DE102021113602A1 (de) 2021-05-26 2022-12-01 Jacobs University Bremen Ggmbh Gentechnisch veränderte hefe zur biotechnologischen herstellung von bernsteinsäure aus glycerol

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010110217A (ja) * 2007-02-22 2010-05-20 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
WO2010019882A1 (en) * 2008-08-15 2010-02-18 Edeniq, Inc. Genetically-engineered yeast and methods of making and using
CN102666862A (zh) * 2009-08-21 2012-09-12 马斯科马公司 在联合生物加工生物体中生产丙醇、醇和多元醇
US8772000B2 (en) * 2010-04-19 2014-07-08 Korea University Research And Business Foundation Transformant for enhancing bioethanol production, and method for producing ethanol by using said strain
WO2011149353A1 (en) * 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
FR2962133B1 (fr) * 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
PL2663645T3 (pl) * 2010-11-18 2015-05-29 Dsm Ip Assets Bv Szczepy drożdży zmodyfikowane do wytwarzania etanolu z glicerolu
EP3321368A3 (de) * 2011-11-30 2018-05-23 DSM IP Assets B.V. Zur herstellung von ethanol aus acetatsäure und glycerin manipulierte hefestämme
WO2014074895A2 (en) * 2012-11-09 2014-05-15 Mascoma Corporation Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks
AR097479A1 (es) * 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado

Also Published As

Publication number Publication date
EP3169768B1 (de) 2018-09-26
EP3169768A1 (de) 2017-05-24
WO2016008819A1 (de) 2016-01-21

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Owner name: CONSTRUCTOR UNIVERSITY BREMEN GGMBH, DE

Free format text: FORMER OWNER: JACOBS UNIVERSITY BREMEN GGMBH, 28759 BREMEN, DE