DE10318026A1 - Lighting device in a cryostat - Google Patents
Lighting device in a cryostat Download PDFInfo
- Publication number
- DE10318026A1 DE10318026A1 DE10318026A DE10318026A DE10318026A1 DE 10318026 A1 DE10318026 A1 DE 10318026A1 DE 10318026 A DE10318026 A DE 10318026A DE 10318026 A DE10318026 A DE 10318026A DE 10318026 A1 DE10318026 A1 DE 10318026A1
- Authority
- DE
- Germany
- Prior art keywords
- cryostat
- lighting
- chamber
- lighting modules
- circuit arrangement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D27/00—Lighting arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
- G01N2001/068—Illumination means
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Combustion & Propulsion (AREA)
- Mechanical Engineering (AREA)
- Thermal Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
- Arrangement Of Elements, Cooling, Sealing, Or The Like Of Lighting Devices (AREA)
Abstract
Es wird ein Kryostat (1) mit einer Kryostatkammer (2) beschrieben, in der ein Mikrotom zum Schneiden von gefrorenen Präparaten angeordnet ist. Es ist eine Beleuchtungseinrichtung (4) zum Ausleuchten der Kryostatkammer (2) vorgesehen, wobei die Beleuchtungseinrichtung (4) mindestens eine LED aufweist.A cryostat (1) with a cryostat chamber (2) is described, in which a microtome for cutting frozen preparations is arranged. An illumination device (4) is provided for illuminating the cryostat chamber (2), the illumination device (4) having at least one LED.
Description
Die Erfindung betrifft einen Kryostat mit einer Kryostatkammer in der ein Mikrotom zum Schneiden von gefrorenen Präparaten angeordnet ist, gemäß dem Oberbegriff des Patentanspruchs 1.The The invention relates to a cryostat with a cryostat chamber in the a microtome for cutting frozen preparations is arranged according to the preamble of claim 1.
Derartige Kryostate werden verwendet, um mit einem Mikrotom gefrorene Präparate für eine anschließende Betrachtung mit einem Mikroskop zu schneiden. Die zu schneidenden Präparate werden auf eine bestimmte vorgegebene Temperatur gekühlt werden. Die Temperaturen liegen hierbei in der Regel zwischen -10° C und -50° C. Um die Temperaturkonstanz zu gewährleisten, werden die Mikrotome in aufwendig gekapselten Kryostatkammern angeordnet und diese entsprechend gekühlt.such Cryostats are used to prepare frozen microtome preparations for subsequent viewing cut with a microscope. The preparations to be cut are opened a certain predetermined temperature can be cooled. The temperatures are usually between -10 ° C and -50 ° C. To keep the temperature constant to ensure, the microtomes are arranged in elaborately encapsulated cryostat chambers and these are cooled accordingly.
Um ein sicheres Arbeiten zu gewährleisten, wird die Kryostatkammer mit Lampen beleuchtet. Dies hat jedoch den Nachteil, dass diese Lichtquellen Wärme erzeugen und so die für den Schnitt des Präparats entscheidende Temperaturkonstanz gefährdet ist.Around to ensure safe working the cryostat chamber is illuminated with lamps. However, this has the disadvantage that these light sources heat generate and so the for the section of the preparation decisive temperature constancy is at risk.
Außerdem hat es sich herausgestellt, dass sich die recht groß dimensionierten Lichtquellen nur schlecht reinigen lassen.Also has it turned out that the light sources are quite large poorly cleaned.
Es ist daher Aufgabe der vorliegenden Erfindung, die Beleuchtung der Kryostatkammer so zu verbessern, dass eine höhere Temperaturkonstanz innerhalb der Kammer gegeben ist.It is therefore an object of the present invention, the lighting of the To improve the cryostat chamber so that a higher temperature constancy within the chamber is given.
Diese Aufgabe wird erfindungsgemäß durch die im kennzeichnenden Teil des Patentanspruches 1 angegebenen Merkmale gelöst. Weitere vorteilhafte Weiterbildungen der Erfindung sind Gegenstand der Unteransprüche.This The object is achieved by the features specified in the characterizing part of claim 1 solved. Further advantageous developments of the invention are the subject of subclaims.
Die Erfindung zeichnet sich dadurch aus, dass die Beleuchtungseinrichtung als Lichtquelle mindestens eine LED, vorzugsweise eine Weißlicht – LED, aufweist. Mit der LED wird eine kompakte Bauform, eine sehr geringe Wärmeabstrahlung und eine gute Reinigungs- und Desinfektionsmöglichkeit erreicht. Außerdem lassen sich die LEDs durch ihre Raumform auch sehr gut gegen Spritzwasser schützen.The The invention is characterized in that the lighting device has at least one LED, preferably a white light LED, as the light source. The LED is a compact design, very little heat radiation and achieved good cleaning and disinfection options. Let also Due to their spatial shape, the LEDs are also very good against splashing water protect.
In einer Ausgestaltung der Erfindung sind mehrere LEDs zu einem Beleuchtungsmodul zusammengefasst. Damit wird eine erhöhte Leuchtdichte bei einer kompakten Bauform erreicht. Ferner lassen sich so auch die elektrischen Kontakte der LEDs sehr einfach gegen Feuchtigkeit bzw. Spritzwasser schützen.In In one embodiment of the invention, a plurality of LEDs form a lighting module summarized. This results in an increased luminance at compact design achieved. Furthermore, the electrical can be Contacts of the LEDs very easily against moisture or splash water protect.
In einer Weiterbildung der Erfindung weist das Beleuchtungsmodul ein Gehäuse mit einem Steckeranschluss zur Stromversorgung auf. Damit wird erreicht, dass das Beleuchtungsmodul über den Steckeranschluss austauschbar in der Kryostatkammer angeordnet werden kann. So können, je nach Bedarf, einzelne oder mehrere Beleuchtungsmodule in vorbereitete Steckeraufnahmen in der Kryostatkammer austauschbar angeordnet werden.In In a development of the invention, the lighting module casing with a plug connection for power supply. So that is achieved that the lighting module over the connector is arranged interchangeably in the cryostat chamber can be. So can Depending on requirements, single or multiple lighting modules in prepared Connector receptacles can be arranged interchangeably in the cryostat chamber.
In einer weiteren Ausgestaltung der Erfindung sind mehrere Beleuchtungsmodule elektrisch mit einer gemeinsamen Stromversorgung verbunden. Diese Stromversorgung ist vorzugsweise außerhalb der Kryostatkammer vorgesehen, so dass neben einem minimierten Aufwand hier auch eine reduzierte Wärmeentwicklung realisiert ist.In Another embodiment of the invention is a plurality of lighting modules electrically connected to a common power supply. This Power supply is preferably outside the cryostat chamber provided, so that in addition to minimized effort here also reduced heat development is realized.
In einer Weiterbildung der Erfindung ist eine Schaltungsanordnung zum An- und Abschalten einzelner und/oder mehrerer Beleuchtungsmodule vorgesehen. Je nach Anwendung können so einzelne Bereiche innerhalb der Kryostatkammer beleuchtet werden.In A further development of the invention is a circuit arrangement for Switching on and off individual and / or several lighting modules intended. Depending on the application individual areas within the cryostat chamber are illuminated.
In einer vorteilhaften Weiterbildung hat die Schaltungsanordnung zusätzlich die Funktion eines Dimmers für einzelner und/oder mehrerer Beleuchtungsmodule.In In an advantageous further development, the circuit arrangement additionally has the Function of a dimmer for individual and / or multiple lighting modules.
Dadurch ist ebenfalls eine individuelle Ausleuchtung der Kryostatkammer möglich.Thereby is also an individual illumination of the cryostat chamber possible.
In einer Weiterbildung der Erfindung ist am Kryostat ein Schalter angeordnet und mit der Schaltungsanordnung elektrisch verbunden ist, wobei der Schalter beim Öffnen und/oder Schließen der Kryostatkammer einzelne und/oder mehrere Beleuchtungsmodule automatisch schaltet und/oder dimmt. Mit dieser Funktion wird erreicht, dass beim Öffnen der Deckels oder der Scheibe durch eine Bedienperson automatisch das Beleuchtungslicht innerhalb der Kammer gesteuert wird. So ist es beispielsweise möglich, beim Öffnen immer auf optimale Beleuchtung zu Schalten und beim Schließen einzelne Beleuchtungsmodule auszuschalten oder zu dimmen. So erscheint es sinnvoll, bei geschlossener Kryostatkammer nur den Probenhalter und/oder das Schneidmesser des Mikroatoms zu beleuchten.In In a further development of the invention, a switch is arranged on the cryostat and is electrically connected to the circuit arrangement, wherein the switch when opening and / or closing the cryostat chamber individual and / or several lighting modules automatically switches and / or dims. With this function it is achieved that when you open the Cover or the disc automatically by an operator Illumination light is controlled within the chamber. That's the way it is for example possible When opening Always switch to optimal lighting and individual when closing Switch off or dim lighting modules. This is how it appears sensible, only the sample holder when the cryostat chamber is closed and / or to illuminate the cutting knife of the micro-dome.
In einer weiteren Ausgestaltung der Erfindung ist mindestens einer LED ein Spritzwasserschutz zugeordnet. Vorteilhaft kann das Beleuchtungsmodul mit einer Dichtung zur Kapselung der elektrischen LED Kontakte ausgestattet sein.In a further embodiment of the invention is at least one LED assigned a splash protection. The lighting module can be advantageous equipped with a seal to encapsulate the electrical LED contacts his.
Die Erfindung wird anhand eines Ausführungsbeispiels mit Hilfe der schematischen Zeichnungen näher erläutert. Es zeigen:The Invention is based on an embodiment explained in more detail with the aid of the schematic drawings. Show it:
Die
Über die
Schaltungsanordnung
Die
Die
Die
- 11
- Kryostatcryostat
- 22
- Kryostatkammercryostat
- 33
- Mikrotommicrotome
- 44
- Beleuchtungseinrichtunglighting device
- 55
- LEDLED
- 66
- Beleuchtungsmodullighting module
- 77
- Gehäusecasing
- 88th
- Steckeranschlussconnector
- 99
- Stromversorgungpower supply
- 1010
- Schaltungsanordnungcircuitry
- 1111
- Schalterswitch
- 1212
- Spritzwasserschutzsplash protection
- 1313
- Platinecircuit board
- 1414
- Leitungencables
- 1515
- Stromleitungpower line
- 1616
- Gefrierleistefreeze shelf
- 1717
- Deckelcover
Claims (8)
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10318026.5A DE10318026C5 (en) | 2003-04-19 | 2003-04-19 | Cryostat with lighting device |
DE20321622U DE20321622U1 (en) | 2003-04-19 | 2003-04-19 | Lighting device in a cryostat |
GB0403255A GB2400645B (en) | 2003-04-19 | 2004-02-13 | Illumination device in a cryostat |
US10/824,211 US20040206093A1 (en) | 2003-04-19 | 2004-04-14 | Illumination device in a cryostat |
JP2004121043A JP2004317515A (en) | 2003-04-19 | 2004-04-16 | Cryostat having lighting device |
CNB2004100368306A CN100498271C (en) | 2003-04-19 | 2004-04-19 | Low temperature thermostat |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10318026.5A DE10318026C5 (en) | 2003-04-19 | 2003-04-19 | Cryostat with lighting device |
Publications (3)
Publication Number | Publication Date |
---|---|
DE10318026A1 true DE10318026A1 (en) | 2004-11-04 |
DE10318026B4 DE10318026B4 (en) | 2010-05-27 |
DE10318026C5 DE10318026C5 (en) | 2015-11-12 |
Family
ID=32010540
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE10318026.5A Expired - Fee Related DE10318026C5 (en) | 2003-04-19 | 2003-04-19 | Cryostat with lighting device |
DE20321622U Expired - Lifetime DE20321622U1 (en) | 2003-04-19 | 2003-04-19 | Lighting device in a cryostat |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE20321622U Expired - Lifetime DE20321622U1 (en) | 2003-04-19 | 2003-04-19 | Lighting device in a cryostat |
Country Status (5)
Country | Link |
---|---|
US (1) | US20040206093A1 (en) |
JP (1) | JP2004317515A (en) |
CN (1) | CN100498271C (en) |
DE (2) | DE10318026C5 (en) |
GB (1) | GB2400645B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102012215859A1 (en) | 2012-09-06 | 2014-03-06 | Leica Biosystems Nussloch Gmbh | Processing device for processing tissue samples with a reader device for user identification units |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4840763B2 (en) * | 2006-01-18 | 2011-12-21 | セイコーインスツル株式会社 | Automatic thin section preparation apparatus and automatic thin section preparation apparatus |
DE102009017848B4 (en) * | 2009-04-17 | 2011-03-17 | Leica Biosystems Nussloch Gmbh | Frozen microtome and method of making microscopic thin sections |
RU2615367C2 (en) * | 2010-03-31 | 2017-04-04 | Хилин Лайф Продактс Инк. | Device for predicting of ovulation |
DE102011002443B4 (en) * | 2011-01-04 | 2013-08-29 | Leica Biosystems Nussloch Gmbh | Cryostat with lighting device |
Family Cites Families (26)
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GB1518792A (en) * | 1975-11-04 | 1978-07-26 | South London Elect Equip | Cryostats |
DE2906153C2 (en) * | 1979-02-17 | 1984-10-31 | C. Reichert Optische Werke Ag, Wien | Cooling chamber for holding objects to be processed, in particular biological objects |
DE3615713C1 (en) * | 1986-05-09 | 1987-09-03 | Bernhard Dr Wolf | Microtome |
AT390512B (en) * | 1986-10-14 | 1990-05-25 | Sitte Hellmuth | REFRIGERATION CHAMBER FOR MAKING THIN CUTS AT A REDUCED TEMPERATURE |
DE8812708U1 (en) * | 1988-10-10 | 1989-01-05 | Cambridge Instruments GmbH, 6907 Nußloch | Microtome device |
DE19527168A1 (en) * | 1995-07-25 | 1997-01-30 | Robert Bodenstedt Elekronik | Illuminating device for bicycle rear light |
DE19803966B4 (en) * | 1997-03-07 | 2006-05-11 | Microm International Gmbh | Suction device for cutting waste in a cryostat microtome and cryostat microtome |
US6152590A (en) * | 1998-02-13 | 2000-11-28 | Donnelly Hohe Gmbh & Co. Kg | Lighting device for motor vehicles |
EP0971186B1 (en) * | 1998-07-08 | 2003-08-20 | Liebherr-Hausgeräte Ochsenhausen GmbH | Interior lighting for refrigerator |
DE19908404C2 (en) * | 1999-02-26 | 2002-01-24 | Airsigna Gmbh & Co Kg | Interior lighting for vehicles, in particular air and water vehicles |
DE29914037U1 (en) * | 1999-08-11 | 2000-12-28 | Diehl Ako Stiftung Gmbh & Co | Indoor lighting for household appliances |
JP2001221951A (en) * | 1999-11-29 | 2001-08-17 | Olympus Optical Co Ltd | Microscope |
DE19961787A1 (en) * | 1999-12-21 | 2001-06-28 | Bsh Bosch Siemens Hausgeraete | Refrigeration device has illumination device with illumination body between transparent, plate-like cover layer and wall with heat resistant section in illumination body radiation region |
ATE330448T1 (en) * | 2000-02-03 | 2006-07-15 | Koninkl Philips Electronics Nv | CIRCUIT ARRANGEMENT FOR AN LED LIGHTING MODULE |
DE10013056B4 (en) * | 2000-03-19 | 2017-09-28 | Osram Opto Semiconductors Gmbh | lighting arrangement |
CH697261B1 (en) * | 2000-09-26 | 2008-07-31 | Lisa Lux Gmbh | Lighting for refrigeration units. |
DE10051159C2 (en) * | 2000-10-16 | 2002-09-19 | Osram Opto Semiconductors Gmbh | LED module, e.g. White light source |
CA2336497A1 (en) * | 2000-12-20 | 2002-06-20 | Daniel Chevalier | Lighting device |
US6472823B2 (en) * | 2001-03-07 | 2002-10-29 | Star Reach Corporation | LED tubular lighting device and control device |
US6481219B2 (en) * | 2001-03-30 | 2002-11-19 | Sakura Finetek U.S.A., Inc. | Disinfection system and method of using same |
US6578986B2 (en) * | 2001-06-29 | 2003-06-17 | Permlight Products, Inc. | Modular mounting arrangement and method for light emitting diodes |
DE10147986A1 (en) * | 2001-09-28 | 2003-04-10 | Osram Opto Semiconductors Gmbh | Manufacturing surface mountable light emitting diode light source involves bending leadframe reflector section into shape of reflector before or after mounting LED chip |
CZ12316U1 (en) * | 2002-04-25 | 2002-06-10 | K.T.-Kalttech, S.R.O. | Lighting device for refrigerators, particularly for thermoelectric refrigerators |
US6851820B2 (en) * | 2002-06-26 | 2005-02-08 | Weber-Stephen Products Company | Barbecue grill light |
DE10250877B4 (en) * | 2002-10-31 | 2008-09-04 | Osram Opto Semiconductors Gmbh | A semiconductor light-emitting device, manufacturing method and use thereof, a plurality of modules including the semiconductor light-emitting devices, and the use thereof |
DE10303969B4 (en) * | 2003-01-31 | 2008-11-27 | Osram Opto Semiconductors Gmbh | Light-emitting diode arrangement with a light-emitting diode and a plurality of light-emitting diodes |
-
2003
- 2003-04-19 DE DE10318026.5A patent/DE10318026C5/en not_active Expired - Fee Related
- 2003-04-19 DE DE20321622U patent/DE20321622U1/en not_active Expired - Lifetime
-
2004
- 2004-02-13 GB GB0403255A patent/GB2400645B/en not_active Expired - Fee Related
- 2004-04-14 US US10/824,211 patent/US20040206093A1/en not_active Abandoned
- 2004-04-16 JP JP2004121043A patent/JP2004317515A/en active Pending
- 2004-04-19 CN CNB2004100368306A patent/CN100498271C/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102012215859A1 (en) | 2012-09-06 | 2014-03-06 | Leica Biosystems Nussloch Gmbh | Processing device for processing tissue samples with a reader device for user identification units |
US9274034B2 (en) | 2012-09-06 | 2016-03-01 | Leica Biosystems Nussloch Gmbh | Processing apparatus for processing tissue samples having a reading device for user identification units |
Also Published As
Publication number | Publication date |
---|---|
CN100498271C (en) | 2009-06-10 |
DE10318026B4 (en) | 2010-05-27 |
US20040206093A1 (en) | 2004-10-21 |
GB2400645A (en) | 2004-10-20 |
DE20321622U1 (en) | 2008-07-17 |
CN1538160A (en) | 2004-10-20 |
GB0403255D0 (en) | 2004-03-17 |
DE10318026C5 (en) | 2015-11-12 |
JP2004317515A (en) | 2004-11-11 |
GB2400645B (en) | 2005-06-29 |
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Legal Events
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8127 | New person/name/address of the applicant |
Owner name: LEICA BIOSYSTEMS NUSSLOCH GMBH, 69226 NUSSLOCH, DE |
|
8110 | Request for examination paragraph 44 | ||
8363 | Opposition against the patent | ||
R006 | Appeal filed | ||
R008 | Case pending at federal patent court | ||
R010 | Appeal proceedings settled by withdrawal of appeal(s) or in some other way | ||
R034 | Decision of examining division/federal patent court maintaining patent in limited form now final | ||
R206 | Amended patent specification | ||
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |