DE102020120190A1 - Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop - Google Patents

Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop Download PDF

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Publication number
DE102020120190A1
DE102020120190A1 DE102020120190.7A DE102020120190A DE102020120190A1 DE 102020120190 A1 DE102020120190 A1 DE 102020120190A1 DE 102020120190 A DE102020120190 A DE 102020120190A DE 102020120190 A1 DE102020120190 A1 DE 102020120190A1
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DE
Germany
Prior art keywords
pixel
matrix sensor
pixels
spectral
emission light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE102020120190.7A
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German (de)
English (en)
Inventor
Daniel Schwedt
Tiemo Anhut
Peter Schacht
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Microscopy GmbH filed Critical Carl Zeiss Microscopy GmbH
Priority to DE102020120190.7A priority Critical patent/DE102020120190A1/de
Priority to EP21752522.9A priority patent/EP4189358B1/de
Priority to US18/018,331 priority patent/US11921274B2/en
Priority to PCT/EP2021/071269 priority patent/WO2022023474A1/de
Priority to JP2023506011A priority patent/JP2023537295A/ja
Priority to CN202180058676.4A priority patent/CN116249891A/zh
Publication of DE102020120190A1 publication Critical patent/DE102020120190A1/de
Withdrawn legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/027—Control of working procedures of a spectrometer; Failure detection; Bandwidth calculation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/18—Generating the spectrum; Monochromators using diffraction elements, e.g. grating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G01J2003/2813—2D-array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
DE102020120190.7A 2020-07-30 2020-07-30 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop Withdrawn DE102020120190A1 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE102020120190.7A DE102020120190A1 (de) 2020-07-30 2020-07-30 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop
EP21752522.9A EP4189358B1 (de) 2020-07-30 2021-07-29 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laserscanning-mikroskop
US18/018,331 US11921274B2 (en) 2020-07-30 2021-07-29 Method for detecting emission light, detection device and laser scanning microscope
PCT/EP2021/071269 WO2022023474A1 (de) 2020-07-30 2021-07-29 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laserscanning-mikroskop
JP2023506011A JP2023537295A (ja) 2020-07-30 2021-07-29 放射光検出方法、検出装置及びレーザ走査顕微鏡
CN202180058676.4A CN116249891A (zh) 2020-07-30 2021-07-29 探测发射光的方法、探测设备和激光扫描显微镜

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102020120190.7A DE102020120190A1 (de) 2020-07-30 2020-07-30 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop

Publications (1)

Publication Number Publication Date
DE102020120190A1 true DE102020120190A1 (de) 2022-02-03

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DE102020120190.7A Withdrawn DE102020120190A1 (de) 2020-07-30 2020-07-30 Verfahren zum detektieren von emissionslicht, detektionsvorrichtung und laser-scanning-mikroskop

Country Status (6)

Country Link
US (1) US11921274B2 (https=)
EP (1) EP4189358B1 (https=)
JP (1) JP2023537295A (https=)
CN (1) CN116249891A (https=)
DE (1) DE102020120190A1 (https=)
WO (1) WO2022023474A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102023100926A1 (de) 2023-01-17 2024-07-18 Carl Zeiss Microscopy Gmbh Mikroskop
DE102023209080A1 (de) 2023-09-19 2025-03-20 Carl Zeiss Microscopy Gmbh Multimodales Scanmikroskop und Verfahren zu dessen Betrieb

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7402313B2 (ja) * 2020-03-27 2023-12-20 ギガフォトン株式会社 センサ劣化判定方法
EP4718138A1 (en) * 2024-09-25 2026-04-01 Leica Microsystems CMS GmbH Detection arrangement, cascaded detection arrangement, and optical scanning microscope

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140361154A1 (en) 2013-06-11 2014-12-11 Olympus Corporation Confocal image generation apparatus
DE102014111167A1 (de) 2014-08-06 2016-02-11 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie mit der Unterscheidung mindestens zweier Wellenlängenbereiche
DE102017113683A1 (de) 2017-06-21 2018-12-27 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie mit der Unterscheidung mindestens zweier Wellenlängenbereiche
US20190258041A1 (en) 2016-10-17 2019-08-22 Carl Zeiss Microscopy Gmbh Optical Group For Detection Light for a Microscope, Method for Microscopy, and Microscope

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10127284A1 (de) * 2001-06-05 2002-12-12 Zeiss Carl Jena Gmbh Autofokussiereinrichtung für ein optisches Gerät
DE10339312A1 (de) * 2003-08-27 2005-03-31 Leica Microsystems Heidelberg Gmbh Verfahren zur Trennung von Fluoreszenzspektren von in einer Probe vorhandenen Farbstoffen
FR2966258B1 (fr) * 2010-10-15 2013-05-03 Bioaxial Système de microscopie de superresolution de fluorescence et méthode pour des applications biologiques
DE102016120308A1 (de) * 2016-10-25 2018-04-26 Carl Zeiss Microscopy Gmbh Optische Anordnung, Multispot-Scanning-Mikroskop und Verfahren zum Betreiben eines Mikroskops
EP3460520B1 (en) * 2017-09-25 2023-07-19 Hexagon Technology Center GmbH Multi-beam laser scanner
IT201800001891A1 (it) * 2018-01-25 2019-07-25 Fondazione St Italiano Tecnologia Metodo di imaging risolto nel tempo ad alta risoluzione spaziale.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140361154A1 (en) 2013-06-11 2014-12-11 Olympus Corporation Confocal image generation apparatus
DE102014111167A1 (de) 2014-08-06 2016-02-11 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie mit der Unterscheidung mindestens zweier Wellenlängenbereiche
US20190258041A1 (en) 2016-10-17 2019-08-22 Carl Zeiss Microscopy Gmbh Optical Group For Detection Light for a Microscope, Method for Microscopy, and Microscope
DE102017113683A1 (de) 2017-06-21 2018-12-27 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie mit der Unterscheidung mindestens zweier Wellenlängenbereiche

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CASTELLO, Marco [u.a.]: Image scanning microscopy with single-photon detector array. In: bioRxiv. 02-06-2018. 21 S. DOI: 10.1101/335596. URL: https://www.biorxiv.org/content/10.1101/335596v1.full.pdf [abgerufen am 2020-09-07]
STRASSER, Franziska, et al.: Spectral image scanning microscopy. In: Biomedical optics express, 2019, 10. Jg., Nr. 5, S. 2513-2527. URL: https://doi.org/10.1364/BOE.10.002513 [abgerufen am 2021-04-14]

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102023100926A1 (de) 2023-01-17 2024-07-18 Carl Zeiss Microscopy Gmbh Mikroskop
WO2024153476A1 (de) 2023-01-17 2024-07-25 Carl Zeiss Microscopy Gmbh Mikroskop
DE102023209080A1 (de) 2023-09-19 2025-03-20 Carl Zeiss Microscopy Gmbh Multimodales Scanmikroskop und Verfahren zu dessen Betrieb
WO2025061458A1 (de) 2023-09-19 2025-03-27 Carl Zeiss Microscopy Gmbh Multimodales scanmikroskop und verfahren zu dessen betrieb

Also Published As

Publication number Publication date
US20230258916A1 (en) 2023-08-17
US11921274B2 (en) 2024-03-05
WO2022023474A1 (de) 2022-02-03
EP4189358A1 (de) 2023-06-07
JP2023537295A (ja) 2023-08-31
CN116249891A (zh) 2023-06-09
EP4189358B1 (de) 2024-07-10

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R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee