DE102010016134A1 - Producing a concentrate from camel milk, useful in pharmaceutical or cosmetic composition for treating skin diseases, comprises preparing cream from camel milk, stirring the cream and separating the camel milk water - Google Patents

Abstract

Producing a concentrate from camel milk comprises preparing cream from camel milk, stirring the cream with 30 to a maximum of 120 revolutions per minute until the concentrate is formed from the cream and separating the camel milk water. ACTIVITY : Dermatological. MECHANISM OF ACTION : None given.

Description

The invention relates to a method for producing a camel's milk concentrate for pharmaceutical or cosmetic compositions.

Milk contains all the important components of human food, such as carbohydrates, proteins, fats, vitamins and minerals. It is for example from the DE 26 23 250 A1 It is known that milk or its components can also be applied externally to the skin and then have a nourishing or healing effect. Therefore, there are many cosmetic preparations based on milk, such as ointments, creams, shampoos and soaps. That's how it describes it U.S. Patent 5,053,219 cosmetic products containing nourishing proteins from milk. Such products are usually made from cow's milk, which is available in large quantities for industrial purposes.

However, the nature and proportions of the individual ingredients of milk vary from species to species. Therefore, it can be expected that the properties and effects may vary depending on the origin of the milk. Accordingly, in the European patent application EP 17 85 037 A1 a dairy product made from mares and goat's milk. The WO 2005/023208 discloses a cosmetic composition of camel milk.

Although the mechanisms of action have not yet been scientifically clarified, raw camel milk is said to have positive effects in diseases of various kinds. This has been observed for example in allergies, gastrointestinal diseases and skin diseases. Therefore, there is a great need to use camel milk or its ingredients for pharmaceutical or cosmetic compositions. However, such products could not prevail so far, because the known production processes for cow's milk for camel milk are not suitable. This is due to the fact that the effectiveness of the ingredients of the sensitive camel's milk during or in part lost during unsuitable manufacturing processes. Thus, in the known extraction, compression and preservation processes, temperatures are used which damage or destroy the ingredients, e.g. As in the pasteurization, milk powder production or condensed milk. Other known separation processes take place under high pressure, z. In osmosis, high pressure separation and high speed spinning. Since milk is present as an emulsion, which has so-called micelles with lipophilic and hydrophilic components, their complex structure but also other milk components are damaged or denatured by the forces acting.

The object of the invention is therefore to propose a method by which a particularly gentle production of a concentrate of camel milk for pharmaceutical or cosmetic compositions is made possible.

This object is achieved according to claim 1, characterized in that camel milk is first made cream. The cream is then stirred at 30 to a maximum of 120 revolutions per minute until a concentrate forms and separates from the camel milk water or settles. Surprisingly, it has been found that the cream forms a concentrate by the very slow stirring, which contains predominantly their solid constituents, and an aqueous phase, which is referred to below as camel milk water. The separation is done very gently by the slow stirring. Experiments have shown that at higher speeds than 120 revolutions per minute a stronger mechanical load and thus damage to proteins and other ingredients of the cream occur. The concentrate obtained contains the non-aqueous components of camel's milk in concentrated form. The concentrate obtained is pure white in color and has a hard consistency at room temperature which is approximately comparable to that of coconut fat.

A high yield and high quality of the concentrate are achieved when stirred at room temperature and the stirring time is preferably about 12 hours.

The cream needed for the stirring is prepared by heating raw camel's milk at 60 ° C for one hour to kill unwanted microorganisms. This temperature is well below the usual pasteurization temperature for cow's milk, which is heated to 72 to 75 ° C for 15 to 30 seconds. In the method according to the invention, however, the lower temperature is compensated by the longer heating time of one hour.

The camel's milk so treated is then placed in a milk separator to separate the cream from the camel's skim milk by means of the milk separator. The temperature drops quickly in the milk separator. Cream and skimmed milk only have a temperature of about 40 degrees Celsius or less when dispensed from the milk separator. Thereafter, the resulting cream is cooled down to at least 10 ° C. This increases the quality of the final product. The advantage of this process step is that the temperature is kept so low that the ingredients of camel milk are largely retained in their native and effective form. The term milk separator includes the terms milk centrifuge, skimming separator or skimming centrifuge or any other device suitable for separating milk and cream. For the purposes of the invention, raw camel milk refers to milk that has not been further treated after milking.

Alternatively, raw camel milk is stored for about 48 hours at a temperature of about 4 ° C until a fat supernatant settles as cream, which is skimmed off for further processing. On the one hand, camel milk is exposed to an even lower temperature, on the other hand, mechanical effects such as high pressures or shearing forces are completely avoided. As a result, the cream is not exposed to any thermal and mechanical treatment and contains all ingredients in their native and effective form.

In the next step, the cream is added to food-safe and skin-friendly preservatives, preferably citric acid and / or potassium sorbate. The mass fraction of citric acid is a maximum of 0.25 g / 100 g of cream (corresponds to 0.25 wt .-%) and the mass fraction of potassium sorbate at a maximum of 0.5 g / 100 g of cream (corresponds to 0.5 wt .-%) , This ensures the durability of the end product with good skin compatibility.

The concentrate thus obtained has a large proportion of the non-aqueous components of the camel's milk, the water content being only about 20 g of water in 100 g of concentrate. The concentrate can be used directly as a base or as an active ingredient for pharmaceutical or cosmetic compositions.

Accordingly, the invention includes pharmaceutical or cosmetic compositions comprising a concentrate prepared by the method described.

The invention further comprises the use of the concentrate or the use of pharmaceutical or cosmetic compositions comprising the concentrate for the topical treatment of skin diseases. It has surprisingly been found that even the external application of the concentrate on the skin alone, d. H. has no other active ingredients, soothing or healing effects on various injuries or skin disorders. Examples are given below.

The reason for this is assumed that the method described above provides a particularly gentle extraction and thereby preserves natural active ingredients of camel milk unchanged or virtually unchanged. The maximum temperature to which the active ingredients are exposed never exceeds 60 ° C during the manufacturing process. This preserves all ingredients, especially proteins, in their native structure. In addition, the non-aqueous components of camel milk are particularly concentrated, and more so than is possible by the methods known for cow's milk. It is believed that the strong concentration of the active ingredients also increases their effectiveness.

The concentrate is preserved by means of food-safe and skin-friendly preservatives and is suitable for further processing into pharmaceutical or cosmetic products, which thereby have nourishing or healing effects of camel milk. Suitable pharmaceutical or cosmetic compositions may be, for example, ointments, creams, pastes, lotions, shampoos and the like.

Exemplary embodiment of the production method

• a) Camel milk is milked in steel buckets cleaned with hot water and poured through a sieve into pre-cleaned, closed milk churns. The milk churns are then stored deep-frozen or processed immediately.
• b) Prior to further processing, each individual jug is taken approximately a 50 ml sample, which is examined, inter alia, for color, taste, odor, acidity, germs, cell count and fat content. Following a sorting process takes place. Camel milk with a lower fat content than 4 wt .-% is used for food production, the camel milk with a fat content of at least 4 wt .-% is used for the preparation of the concentrate according to the invention.
• c) About 500 liters of camel's milk with a fat content of at least 4% by weight are collected and heated to 60 ° C in a pasteurizer for about one hour.
• d) After heating, place the 500 liters of camel's milk in a semi-automatic milk separator, where it is separated into camel's skimmed milk and cream. The separation process takes about two hours. This produces about 450 liters of camel skim milk with a fat content of 1.0-1.2 wt .-% and about 50 liters of cream. The camel skimmed milk is used for food production, the cream is used for the production of the concentrate according to the invention.
• 4) The cream is added to conserve 0.25 wt .-% citric acid and 0.5 wt .-% potassium sorbate and then stored cold in clean milk churns at a temperature of 4 ° C.
• 5) Stored cream is collected until about 100 liters are available. The 100 liters collected cream are filled into the container of a stirring machine and this closed. The cream is then slowly stirred at about 30 rpm to a maximum of 120 rpm at room temperature. As a result, a solid concentrate slowly separates from the camel milk water. The concentrate settles in solid form at the edge of the container. This process is completed in about 12 hours.
• 6) The deposited concentrate is cut out of the mixer by means of steel spoons or steel spatulas and filled into cleaned and ethanol-disinfected transport containers. In this case, about 40 kg of concentrate are obtained from the 100 liters of cream. The camel milk water is no longer used and discarded. Buckets or small plastic barrels with a cleaned plastic bag are used as transport containers. These are tightly closed and stored cold until further transport in the cold store and at a temperature of about 4 ° C.

Properties of the concentrate

The concentrate obtained was laboratory-characterized according to the German Food, Commodities and Feed Code (LFGB). Table 1 shows the results. Table 1: Physico-chemical properties of the concentrate JJL2G water Method: §64 LFGB L06.00-3, mod., Gravimetric water 20.0 g / 100 g JJL1P ash Method: §64 LFGB L06.00-4, mod., Gravimetric Raw ash 0.2 g / 100 g JJL1Q protein Method: §64 LFGB L 06.00-7, mod., Titrimetric (N × 6.25) crude protein 0.8 g / 100 g JJF02 total fat Method: §64 LFGB 106.00-6 mod., Weibull-Stoldt, gravimetric total fat 76.8 g / 100 g JJP1N fatty acid composition Method: DGF C-VI 10a + 11a EC Regulation 1429/92 ISO 15304 , GC-FID C 6: 0 (caproic acid) 0.1% C 8: 0 (caprylic acid) 0.1% C 10: 0 (capric acid) 0.1% C 12: 0 (lauric acid) 0.6% C 14: 0 (myristic acid) 9.1% C 14: 1 (myristoleic acid) + isomers 0.3% C 15: 0 (pentadecanoic acid) 1.7% C 16: 0 (palmitic acid) 31.7% C 16: 1 (palmitoleic acid) + isomers 6.0% C 17: 0 (Margarine acid) 1.1% C 17: 1 (heptadecenoic acid) + isomers 0.6% C 18: 0 (stearic acid) 18.9% C 18: 1 (trans) 3.0% C 18: 1-9 (oleic acid) 18.7% C 18: 1-11 (vaccenic acid) 1.2% C18: 1-13 (13-octadecenoic acid) 0.8% C 18: 2 (trans / trans) 0.2% C 18: 2 (cis / trans) 0.4% C 18: 2 (trans / cis) 0.3% C 18: 2 (linoleic acid) 2.0% C 20: 0 (arachidic acid) 0.5% C 18: 3 (cis / cis / trans) 0.1% C 20: 1 (eicosenoic acid) + isomers 0.2% C 18: 3 (linolenic acid 9, 12, 15 alpha) 1.4% C 20: 2 (eicosadienoic acid) + isomers 0.1% C 22: 0 (behenic acid) 0.1% C 20: 4 (arachidonic acid) 0.1% C 20: 5 (eicosapentaenoic acid) 0.1% C 22: 5 (docosapentaenoic acid) 0.2% Total saturated fat 64.1% total unsaturated fatty acids 27.8% total polyunsaturated fatty acids 3.9% total trans fatty acids 4.0% other 0.2% J3083 Total dietary fiber Method: §64 LFGB L00.00-18, gravimetric total dietary fiber 2.9 g / 100 g J8286 Sugar spectrum Method: §64 LFGB L40.00-7, mod., HPLC / RID fructose <0.5 x g / 100 g glucose <0.5 x g / 100 g sucrose <0.5 x g / 100 g maltose <0.5 x g / 100 g lactose 1.2 g / 100 g JJL2E carbohydrates Method: calculated Carbohydrates calculated (difference) -0.2 g / 100 g JJL1Y nutritional values in 100 g Method: calculated condensing 2861 kJ condensing 696 kcal Calorific value (in accordance with Directive 2008/100 / EC) 2884 kJ Calorific value (in accordance with Directive 2008/100 / EC) 702 kcal protein 0.8 g Carbohydrates # 1.2 g of which sugars 1.2 g fat 76.4 g hereof: saturated fatty acids 49.0 g roughage 2.9 g sodium 0.01 g A7291 Vitamin C Method: HPLC, rp-HPLC-FLD vitamin C <0.5 mg / 100 g J1001 sample preparation Method: §64 LFGB 100.00-19 / 1, pressure digestion J1048 Sodium (Na) Method: DIN EN ISO 11885 , mod., ICP-OES Sodium (Na) 100 mg / kg

It can be seen from the table that the concentrate contains numerous skin-active ingredients, such as. B. a high proportion of different fatty acids.

Examples of the effectiveness of the concentrate

It is known that milk contains various types of proteins, including various caseins, albumins and immunoglobulins. In cow's milk, four immunoglobulin classes have been detected, namely IgG, IgA, IgM and IgE. Immunoglobulins are antibodies which, for example, can have an antibacterial effect. It is also known that milk of different mammalian species differs significantly in composition. In particular, it has differences in the amount and composition of its proteins.

• Fall 1: Proband Nr. 1; Geschlecht männlich; Datum des Vorfalls Oktober 2008. Symptome: Mittlere handtellergroße Hautverbrennung ersten Grades durch heißen Wasserdampf am Bauch. Behandlung: Auftragen des Konzentrats ca. eine Stunde nach der Verletzung. Ergebnis: Sofortige Schmerzfreiheit, nach drei Stunden Rückbildung der Wunde auf 5 × 5 cm, keine Blasenbildung, keine weiteren Beschwerden, völlige Heilung nach zwei Tagen.
• Fall 2: Proband Nr. 1, Geschlecht männlich, Datum des Vorfalls August 2009. Symptome: Drei punktförmige, 1 × 1 cm große Verbrennungen zweiten Grades am Unterarm durch spritzendes heißes Fett mit sofortiger Blasenbildung, die große Schmerzen verursachen. Behandlung: Auftragen des Konzentrats kalt aus dem Kühlschrank; zweite Behandlung mit demselben Konzentrat nach 6 Stunden auf offene Hautwunden. Ergebnis: Schmerzfreiheit nach ca. 6 Stunden; nach zweiter Behandlung der Haut an offenen Blasen sofortige Schmerzfreiheit, keine Entzündung, kein Wässern der Wunden, Brandnarben nach einer Woche völlig abgeheilt ohne Narbenbildung.
• Fall 3 Proband Nr. 2, Geschlecht weiblich, Alter 15 Monate, Datum des Vorfalls März 2009. Symptome: Offene Hautwunden am Po durch Windeln und Urin, drei Tage alt ohne Heilungserfolg mittels herkömmlicher Babycremes. Behandlung: Auftragen des Konzentrats kalt aus dem Kühlschrank vor dem Schlafengehen. Ergebnis: Vermutlich sofortige Schmerzfreiheit, da kein Weinen und ein ruhiger Schlaf beobachtet wurde, offene Hautwunden am nächsten Morgen verheilt, vollständige Heilung während des nächsten Tages.
• Fall 4 Proband Nr. 3, Geschlecht weiblich, Datum des Vorfalls Oktober 2009. Symptome: Schürfwunden an Knien durch Sturz auf Betonboden bei erheblicher Wundverschmutzung. Behandlung: Auswaschen der Wunden mit kalter Kamelmilch und danach Auftragen des Konzentrates. Ergebnis: Sofortige Schmerzfreiheit, keine Entzündung der Wunden, vollständige Heilung nach ca. drei Tagen.
• Fall 5: Proband Nr. 1; Geschlecht männlich; Datum des Vorfalls Oktober 2009. Symptome: Schwere allergische Reaktion mit Rötung, Pusteln, Juckreiz bis zum Aufkratzen am gesamten Körper durch Schwimmen in hochchloriertem Wasser. Behandlung: Einreiben des Körpers mit dem Konzentrat, wiederholtes Einreiben nach einem Tag, weil Allergie wieder auftrat. Ergebnis: Sofortiger Stopp des Juckreizes, Abschwellen der geröteten und entzündeten Hautstellen innerhalb einer Stunde.
• Fall 6: Proband Nr. 4; Geschlecht weiblich; Datum des Vorfalls November 2009. Symptome: Mittelschwerer Sonnenbrand mit Verbrennungen zweiten Grades, starke Rötung der Haut an Gesicht, Schulter und Brust mit teilweise kleinen Bläschen. Behandlung: Einreiben der verbrannten Stellen mit Konzentrat. Ergebnis: Sofortige Schmerzfreiheit, Abklingen der Rötung und Abheilung innerhalb von 24 Stunden.
• Fall 7: Proband Nr. 4; Geschlecht weiblich; Datum des Vorfalls November 2009. Symptome: Reagiert allergisch auf bekannte Gesichtcremes. Behandlung: Fortgesetzte Nutzung des Konzentrates als abendliche Gesichtcreme. Ergebnis: Auffällige Glättung und Straffung der Gesichtshaut nach einer Woche.
• Fall 8: Proband Nr. 5; Geschlecht männlich; Alter 3 Jahre, Afrikaner, stammt aus Dürregebiet Nordkenias und wurde von Großmutter unter extrem schweren Bedingungen aufgezogen; zeigt starke Zeichen von Unterernährung und verlangsamter Entwicklung; körperlicher und geistiger Entwicklungsstand vergleichbar mit 1½ jährigem Kind; Zeitraum der Behandlung Oktober bis November 2009. Symptome: Extrem verschmutzte und vernarbte Haut am gesamten Körper, Ekzeme am gesamten Körper, viele offene oder verschärfte Hautwunden (Kratzwunden), Haut schlaff und grau, bei Massage hoher Abrieb von Hautzellen. Behandlung: 1 × tägliches Auftragen des Konzentrates nach dem Waschen über zwei Monate, dazu regelmäßiges Essen und ausgewogene Diät Ergebnis: Nach drei Tagen sichtbare Verbesserung des Allgemeinzustandes der Haut und Stopp des Juckreizes und Kratzens, nach einer Woche Rückbildung der Ekzeme und sichtbare Heilung der Wunden, nach zwei Wochen normale Hautfarbe.
• Fall 9: Proband Nr. 6; Geschlecht männlich; Datum des Vorfalls Januar 2010. Symptome: Stark juckender Ausschlag im Gesicht, tritt regelmäßig jährlich wiederkehrend nach mehrere Jahre zurückliegendem einmaligem Genuss von Orangen im Spanienurlaub auf. Behandlung: Regelmäßiges Auftragen des Konzentrates über drei Tage nach jedem Waschen des Gesichts. Ergebnis: Sofortiger Stopp des Juckreizes und dadurch Verhinderung von Aufkratzen bzw. Bakterienschmierinfektion, Heilung innerhalb von drei Tagen; (die Heilungsdauer mit herkömmlichen Mitteln beträgt nach Erfahrung des Probanden zwei bis drei Wochen).

In the following case studies, it was investigated whether the concentrate prepared by the described process alone, ie without further additives, already has nourishing or healing effects.

• Case 1: subject no. 1; Gender Male; Date of the incident October 2008. Symptoms: Medium-sized skin burn of the first degree due to hot steam on the stomach. Treatment: Apply the concentrate about one hour after the injury. Result: Immediate freedom from pain, after three hours regression of the wound to 5 × 5 cm, no blistering, no further discomfort, complete healing after two days.
• Case 2: Subject # 1, Gender Male, Date of Incidence August 2009. Symptoms: Three punctate, 1 × 1 cm second degree burns to the forearm by splashing hot fat with immediate blistering that causes severe pain. Treatment: applying the concentrate cold from the refrigerator; second treatment with the same concentrate after 6 hours on open skin wounds. Result: freedom from pain after approx. 6 hours; after the second treatment of the skin on open blisters immediate freedom from pain, no inflammation, no watering of the wounds, burn scars completely healed after one week without scarring.
• Case 3 subject No. 2, gender female, age 15 months, date of the incident March 2009. Symptoms: Open skin wounds on the buttocks by diapers and urine, three days old without healing success using conventional baby creams. Treatment: Apply the concentrate cold from the refrigerator at bedtime. Result: Presumably, immediate freedom from pain as no crying and quiet sleep was observed, open skin wounds healed the next morning, complete healing during the next day.
• Case 4 Subject No. 3, sex female, date of the incident October 2009. Symptoms: Abrasions on knees due to falling on concrete floor with considerable wound contamination. Treatment: Wash out the wounds with cold camel milk and then apply the concentrate. Result: Immediate freedom from pain, no inflammation of the wounds, complete healing after about three days.
• Case 5: subject # 1; Gender Male; Date of the incident October 2009. Symptoms: Severe allergic reaction with redness, pustules, itching to scratching the entire body by swimming in highly chlorinated water. Treatment: rubbing the body with the concentrate, repeated rubbing after one day because allergy reappeared. Result: Immediate stop of the itching, swelling of the reddened and inflamed skin within one hour.
• Case 6: subject No. 4; Gender Female; Date of the incident November 2009. Symptoms: Moderate sunburn with second degree burns, severe reddening of the skin on the face, shoulder and chest, sometimes with small blisters. Treatment: Rubbing the burnt areas with concentrate. Result: Immediate freedom from pain, subsidence of redness and healing within 24 hours.
• Case 7: subject No. 4; Gender Female; Date of the incident November 2009. Symptoms: Allergic to known face creams. Treatment: Continued use of the concentrate as evening face cream. Result: noticeable smoothing and firming of the facial skin after one week.
• Case 8: Subject No. 5; Gender Male; 3 years old, African, native to northern Kenya's drought area, raised by grandmother under extremely severe conditions; shows strong signs of malnutrition and slowed development; physical and mental development comparable to 1½ years old child; Period of treatment October to November 2009. Symptoms: Extremely soiled and scarred skin on the whole body, eczema on the whole body, many open or hardened skin wounds (scratching wounds), skin flabby and gray, with massage high abrasion of skin cells. Treatment: 1 × daily application of the concentrate after washing for two months, with regular food and balanced diet Result: After three days visible improvement of the general condition of the skin and stop of itching and scratching, after one week regression of eczema and visible healing of the wounds , after two weeks normal skin color.
• Case 9: subject No. 6; Gender Male; Date of the incident January 2010. Symptoms: Very itchy rash on the face, occurs regularly annually after several years of last-year enjoyment of oranges in Spain vacation. Treatment: Regular application of the concentrate for three days after each facial wash. Result: Immediate stop of the itching and thus prevention of scratching or bacterial lubrication, healing within three days; (The healing time with conventional means is according to experience of the subject two to three weeks).

The case examples described above all showed a clear effectiveness of the concentrate according to the invention within a short time, when the skin diseases were treated externally. This is a strong indication that, surprisingly, the concentrate alone has soothing or healing dermatological effects, because the concentrate was applied to the skin without further active ingredients or additives.

It can be assumed that the observed effects are based on the particularly gentle production process, which preserves the ingredients of camel milk in their native form and thus can develop their naturally healing effects.

The invention also includes the possibility of adding to the concentrate in a pharmaceutical composition known active substances, such as antibiotics, glucocorticoids or antiallergics and thus to enhance the effect.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited patent literature

• DE 2623250 A1 [0002]
• US 5053219 [0002]
• EP 1785037 A1 [0003]
• WO 2005/023208 [0003]

Cited non-patent literature

• ISO 15304 [0017]
• DIN EN ISO 11885 [0017]

Claims (7)

A process for the preparation of a concentrate of camel milk for pharmaceutical or cosmetic compositions, characterized in that cream is prepared from camel milk and this is stirred at 30 to a maximum of 120 revolutions per minute until a concentrate forms from the cream and separates from the camel milk water. A method according to claim 1, characterized in that it is stirred at room temperature and the stirring time is preferably about 12 hours. A method according to claim 1 or 2, characterized in that the cream is prepared by heating raw camel's milk at 60 ° C for one hour, then placed in a milk separator and separated from the camel's skimmed milk by means of the milk separator. Method according to one of the preceding claims, characterized in that the cream is added before stirring preservative, in particular 0.25 wt .-% citric acid and 0.5 wt .-% potassium sorbate. Pharmaceutical or cosmetic composition, characterized in that it comprises a concentrate prepared according to one of Claims 1 to 4. Use of a pharmaceutical or cosmetic composition according to claim 5 for the treatment of skin diseases. Use of a concentrate prepared according to any one of claims 1 to 4 for the topical treatment of skin diseases.