DE102005020543A1 - Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht - Google Patents

Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht Download PDF

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Publication number
DE102005020543A1
DE102005020543A1 DE102005020543A DE102005020543A DE102005020543A1 DE 102005020543 A1 DE102005020543 A1 DE 102005020543A1 DE 102005020543 A DE102005020543 A DE 102005020543A DE 102005020543 A DE102005020543 A DE 102005020543A DE 102005020543 A1 DE102005020543 A1 DE 102005020543A1
Authority
DE
Germany
Prior art keywords
light
arrangement according
sample
illumination
spectral
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE102005020543A
Other languages
German (de)
English (en)
Inventor
Ralf Wolleschensky
Michael Dr. Kempe
Peter Klopfleisch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
VEB Carl Zeiss Jena GmbH
Carl Zeiss Jena GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VEB Carl Zeiss Jena GmbH, Carl Zeiss Jena GmbH filed Critical VEB Carl Zeiss Jena GmbH
Priority to DE102005020543A priority Critical patent/DE102005020543A1/de
Priority to EP06008984A priority patent/EP1720054A3/de
Priority to JP2006128585A priority patent/JP4865399B2/ja
Priority to US11/416,455 priority patent/US7605976B1/en
Publication of DE102005020543A1 publication Critical patent/DE102005020543A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0224—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using polarising or depolarising elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0229—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using masks, aperture plates, spatial light modulators or spatial filters, e.g. reflective filters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/14—Generating the spectrum; Monochromators using refracting elements, e.g. prisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0068—Optical details of the image generation arrangements using polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0816—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/143—Beam splitting or combining systems operating by reflection only using macroscopically faceted or segmented reflective surfaces
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
    • G02B27/283—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising used for beam splitting or combining
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
DE102005020543A 2005-05-03 2005-05-03 Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht Withdrawn DE102005020543A1 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE102005020543A DE102005020543A1 (de) 2005-05-03 2005-05-03 Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht
EP06008984A EP1720054A3 (de) 2005-05-03 2006-04-29 Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht
JP2006128585A JP4865399B2 (ja) 2005-05-03 2006-05-02 光を調節可能に変化させるための方法および装置
US11/416,455 US7605976B1 (en) 2005-05-03 2006-05-03 Method and device for changing light in an adjustable manner

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102005020543A DE102005020543A1 (de) 2005-05-03 2005-05-03 Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht

Publications (1)

Publication Number Publication Date
DE102005020543A1 true DE102005020543A1 (de) 2006-11-09

Family

ID=36615723

Family Applications (1)

Application Number Title Priority Date Filing Date
DE102005020543A Withdrawn DE102005020543A1 (de) 2005-05-03 2005-05-03 Verfahren und Vorrichtung zur einstellbaren Veränderung von Licht

Country Status (4)

Country Link
US (1) US7605976B1 (https=)
EP (1) EP1720054A3 (https=)
JP (1) JP4865399B2 (https=)
DE (1) DE102005020543A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008049748A1 (de) * 2008-09-30 2009-12-17 Carl Zeiss Ag Strahlteilereinrichtung
DE102012019472A1 (de) * 2012-09-28 2014-04-03 Carl Zeiss Microscopy Gmbh Optische Filtervorrichtung, insbesondere für Mikroskope

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7864423B2 (en) * 2007-08-10 2011-01-04 Aegis Lightwave, Inc. Spectrally adjustable filter
US8284489B2 (en) * 2007-09-11 2012-10-09 Aegis Lightwave, Inc. Spectrally adjustable filter
ITFI20070260A1 (it) * 2007-11-21 2009-05-22 Light 4 Tech Firenze S R L Dispositivo per illuminare un oggetto con una sorgente di luce multispettrale e rivelare lo spettro della luce emessa.
US7817272B2 (en) * 2008-06-09 2010-10-19 Aegis Lightwave, Inc. High-resolution spectrally adjustable filter
WO2011052248A1 (en) * 2009-11-02 2011-05-05 Olympus Corporation Beam splitter apparatus, light source apparatus, and scanning observation apparatus
DE102011077327B4 (de) * 2011-06-09 2022-03-03 Carl Zeiss Microscopy Gmbh Lichtrastermikroskop mit Strahlkombinierer zum Kombinieren von zwei jeweils eigenständig gescannten Beleuchtungsstrahlen
DE102012205722B4 (de) * 2012-04-05 2020-07-23 Carl Zeiss Microscopy Gmbh Abbildendes Farbteilermodul, Mikroskop mit einem solchen Farbteilermodul sowie Verfahren zum Abbilden eines Objektfeldes in eine erste und eine zweite Bildebene
JP2014092682A (ja) * 2012-11-02 2014-05-19 Olympus Corp 顕微鏡用照明装置及びそれを備えた顕微鏡
KR102659810B1 (ko) * 2015-09-11 2024-04-23 삼성디스플레이 주식회사 결정화도 측정 장치 및 그 측정 방법
JP2020515828A (ja) * 2017-04-07 2020-05-28 ヴェリリー ライフ サイエンシズ エルエルシー 落射蛍光集光用のパターン形成光学系
EP4361703A1 (en) * 2022-10-27 2024-05-01 ASML Netherlands B.V. An illumination module for a metrology device

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6167173A (en) 1997-01-27 2000-12-26 Carl Zeiss Jena Gmbh Laser scanning microscope
DE19758744C2 (de) 1997-01-27 2003-08-07 Zeiss Carl Jena Gmbh Laser-Scanning-Mikroskop
WO1999042884A1 (de) 1998-02-19 1999-08-26 Leica Microsystems Heidelberg Gmbh Optische anordnung mit spektral selektivem element
DE19842288A1 (de) 1998-08-04 2000-02-10 Zeiss Carl Jena Gmbh Einstellbare Einkopplung und/oder Detektion einer oder mehrerer Wellenlängen in einem Mikroskop
US6377344B2 (en) 1998-08-04 2002-04-23 Carl Zeiss Jena Gmbh Adjustable coupling in and/or detection of one or more wavelengths in a microscope
DE19936573A1 (de) 1998-12-22 2001-02-08 Zeiss Carl Jena Gmbh Anordnung zur Separierung von Anregungs- und Emissionslicht in einem Mikroskop
DE19923822A1 (de) 1999-05-19 2000-11-23 Zeiss Carl Jena Gmbh Scannende Anordnung, vorzugsweise zur Erfassung von Fluoreszenzlicht
DE19930532C2 (de) * 1999-06-30 2002-03-28 Zeiss Carl Jena Gmbh Anordnung zur Optimierung der Pulsform in einem Laser-Scanning-Mikroskop
US6958811B2 (en) 2000-06-29 2005-10-25 Carl Zeiss Jena Gmbh Method for the detection of dyes in fluorescence microscopy
DE10033180B4 (de) 2000-06-29 2006-08-31 Carl Zeiss Jena Gmbh Verfahren zur Detektion von Farbstoffen in der Fluoreszenzmikroskopie
CA2443664A1 (en) * 2001-04-03 2002-10-17 Cidra Corporation Variable optical source
DE10137155B4 (de) 2001-07-30 2006-11-30 Leica Microsystems Cms Gmbh Optische Anordnung und Scanmikroskop
US6947127B2 (en) 2001-12-10 2005-09-20 Carl Zeiss Jena Gmbh Arrangement for the optical capture of excited and/or back scattered light beam in a sample
US6888148B2 (en) 2001-12-10 2005-05-03 Carl Zeiss Jena Gmbh Arrangement for the optical capture of excited and /or back scattered light beam in a sample
DE10241472B4 (de) * 2002-09-04 2019-04-11 Carl Zeiss Microscopy Gmbh Verfahren und Anordnung zur einstellbaren Veränderung von Beleuchtungslicht und/oder Probenlicht bezüglich seiner spektralen Zusammensetzung und/oder Intensität
DE10247247A1 (de) * 2002-10-10 2004-04-22 Leica Microsystems Heidelberg Gmbh Optische Anordnung und Mikroskop

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008049748A1 (de) * 2008-09-30 2009-12-17 Carl Zeiss Ag Strahlteilereinrichtung
DE102012019472A1 (de) * 2012-09-28 2014-04-03 Carl Zeiss Microscopy Gmbh Optische Filtervorrichtung, insbesondere für Mikroskope
US8988753B2 (en) 2012-09-28 2015-03-24 Carl Zeiss Microscopy Gmbh Optical filter device, in particular for microscopes
DE102012019472B4 (de) 2012-09-28 2023-05-04 Carl Zeiss Microscopy Gmbh Optische Filtervorrichtung, insbesondere für Mikroskope, und Mikroskop

Also Published As

Publication number Publication date
US7605976B1 (en) 2009-10-20
JP4865399B2 (ja) 2012-02-01
EP1720054A3 (de) 2006-11-22
JP2006313356A (ja) 2006-11-16
EP1720054A2 (de) 2006-11-08

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Legal Events

Date Code Title Description
R012 Request for examination validly filed

Effective date: 20120222

R081 Change of applicant/patentee

Owner name: CARL ZEISS MICROSCOPY GMBH, DE

Free format text: FORMER OWNER: CARL ZEISS JENA GMBH, 07745 JENA, DE

Effective date: 20130206

R016 Response to examination communication
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee