DE10103454A1 - Flavonoid concentrate useful in foods, body care products and cosmetics comprises a polysorbate and isoflavones and/or quercetin - Google Patents

Abstract

Flavonoid concentrate comprises a polysorbate and one or more active ingredients selected from genistein, daidzein, glycitein and their glycosides and quercetin or its derivatives. Independent claims are also included for the following: (1) a process for producing the concentrate, comprising adding one or more genistein-type isoflavones to warmed polysorbate and stirring the mixture for about 30 minutes to obtain a sediment-free concentrate; and (2) a process for producing the concentrate, comprising stirring the active ingredients into warmed water, adding polysorbate while stirring at about 100[deg]C, and stirring until a clear concentrate is obtained.

Description

The invention relates to active ingredient concentrates and processes for their preparation.

Hormones are chemical compounds found in special organs or cells synthesized and then transported to another place to be there in very low concentration growth, development and physiological-metabolic Coordinate services.

Plants contain compounds that are not hormonal in nature However, mammals produce hormone-like effects. The best known are so-called isoflavones from legumes. Soy contains e.g. B. genistein, isogenistein and the formononetin. These substances have estrogen effects and therefore one positive influence on postmenopausal symptoms such as circulatory problems, type II Diabetes and osteoporosis, fortunately without any side effects. In order to they indirectly affect the mortality rate. Also general aging phenomena, The skin, for example, belongs to this group of protective properties. In Asia, the average isoflavone intake has been between decades 20 and 100 mg / day, in western countries it is far lower.

Genistein type isoflavones are found in soy and many other plants. Soy-based diet, mainly in the Asian region is common, it is attributed that there are far fewer carcinomas (breast, Prostate, skin) and postmenopausal complaints in women hardly occur. Isoflavones are so-called phytoestrogens and bind to the oestradiol receptor. Animal experiments and skin models showed that these Isoflavones increase the intracellular, enzymatic, antioxidative potential. This is partly attributed to their effect.  

It has long been known that quercetin, which is poorly soluble in water, is a effective radical scavenger. In 1984 Elstner et al were able to show that quercetin Similar to α-tocopherol, for example, certain cell protection functions can take over.

The invention is therefore based on the object, the bioavailability of the increase active ingredients mentioned.

To this end, the invention provides an active ingredient concentrate which is a polysorbate and one or more of the active ingredients genistein, daidcein, glycitein and / or contains their glycosides, quercetin or its derivatives. The invention Concentrate is soluble in water as a clear solution. When administering this Solution, the genistein-type isoflavones in the gastrointestinal tract become easy absorbed. It is therefore z. B. possible, a gift of a soft drink add concentrate according to the invention with the result that the general Isoflavone uptake of the organism almost incidentally and simultaneously with the Drink consumption is increased. Isoflavones of the genistein type in Form of a water-soluble concentrate in the classic food sector and can be used advantageously in the cosmetic or personal care sector. Because the water solubility ensures a significantly increased absorption of the isoflavones through the skin.

It is recommended that the weight ratio of the active ingredient (s) to the Adjust the polysorbate to about 1: 5. With a larger weight ratio, this results a cloudiness or a sediment in an aqueous solution of the concentrate. at Smaller weight ratio, the aqueous solution is clear, however The polysorbate content of the solution exceeds that drawn under food law Border.

Active ingredients of purely vegetable origin are preferred. For a Active ingredient concentrate, which only contains isoflavones of the genistein type, is recommended itself, a generally powdered soybean extract  use 40 g of a mixture of Genistin, Daidcin and Contains glycitin and its aglycones. This is advantageous The quantitative ratio of genistin, daidcin and glycitin then around 1.3: 1.0: 0.3.

Polysorbate 80 is preferably used to produce the concentrate according to the invention. However, polysorbate 20 , polysorbate 40 , polysorbate 60 and polysorbate 65 , which are obtained as partial esters of lauric, palmitic or stearic acid with sorbitol, are also suitable for use for the purpose according to the invention.

In addition to non-alcoholic drinks, the invention therefore also relates to liqueurs, Various foods including skin care products, cosmetics and the like Products that contain a polysorbate isoflavone concentrate and one or more of the active ingredients genistein, daidcein, glycitein and / or their glycosides and / or Contain quercetin or its derivatives. Appropriately contains z. B. one drink per Liter about 0.5 g to about 3 g isoflavone concentrate. This can be done in capsules (1 ml) Concentrate given undiluted as an antioxidant or food supplement become.

To prepare a water-soluble concentrate that contains isoflavones for example, from 100 g of soybean extract powder, which from the Archer-Daniels-Midland Company, USA under the NOVASOY brand is distributed. This soybean extract contains at least 40% by weight of genistin, Daidcin and glycitin and their aglycones in the ratio 1.3: 1.0: 0.3. 100 g of the said extract thus contain 20.0 g of genistin, 15.4 g of daidcin and 4.6 g Glycitin, i.e. a total of 40.0 g isoflavones.

For an agent containing quercetin one can get a quercetin dihydrate proceed from the company SIGMA-ALDRICH-CHEMIE GmbH, Schnelldorf, Germany is sold under item number 83370-100 G.  

For the production of an anhydrous active ingredient concentrate, which isoflavones from Genistin type contains, for example, that serves under the name NOVASOY from the Archer-Daniels-Midland Company, Decatur, Illinois, USA related powdery soy extract, its typical analysis as well Chromatogram are included as an attachment. You can see that this extract mainly contains the glycosides of genistein, daidcein and glycitein.

Approximately 166 g of this soy extract powder are sprinkled in about 834 g of polysorbate 80 heated to about 75 ° C. and the mixture (1.0 kg) is stirred uniformly at this temperature for about half an hour. The result is a deep brown, clear concentrate without sediment. If you add about 1-2 ml of this concentrate to ten times the amount of distilled water at room temperature, a clear solution is obtained.

1.0 kg of this concentrate contains approx. 66 mg isoflavones. By adding 1.5 g of the concentrate to 1.0 l ready drink contains about 100 mg of the above Isoflavones, so that one liter of the finished drink prepared in this way Daily requirements for isoflavones are sufficient.

Instead of NOVASOY you can also use soy extracts from other sources go out, which are characterized by a greater sugar-free. For example is from the company K.-W. Pfannenschmidt GmbH, Hamburg, a soybean extract available that has a total isoflavone content of at least 40%. These about 40% are composed of 7.58% genist, 25.43% genistein, 5.48% daidcin and 1.67% daidcein. Do this with this soybean extract as described above in connection with NOVASOY, is obtained in about the same results.

Above is the preparation of an anhydrous active ingredient concentrate described. However, it is also possible to add an aqueous active ingredient concentrate to get the following examples:  

The recovery of the water-soluble isoflavone concentrate according to the invention provides before that the said compositions in heated to about 60 ° C. Water trickles and the mixture for a predetermined period of stirred for about ten minutes at the temperature mentioned. Then it will Polysorbate added to the mixture and the temperature at about 100 ° C. elevated. At this temperature, the stirring process is continued until the Mixture has become clear and transparent. Alternatively, the Compositions first added polysorbate and then water be added.

For example, 100 g of the soybean extract NOVASOY mentioned are evenly stirred into 400 g of distilled water, which has previously been heated to about 60 ° C. The mixture (total amount 500 g) is constantly stirred for about ten minutes using a magnetic stirrer while maintaining the temperature of about 60 ° C. While continuing the stirring process, 500 g of polysorbate 80 are added and the temperature is increased to about 100 ° C.

The stirring process is continued at this temperature until the mixture (Total amount 1 kg) has become clear and transparent. This clear mix contains the incorporated 40 g isoflavones.

2.5 g of this clear, stable aqueous concentrate contain 100 mg of isoflavones. This amount can, for example, be incorporated into a liter of finished beverage that covers twice the daily requirement of 50 mg isoflavones.

To obtain an aqueous concentrate that only quercetin or of the active ingredients mentioned. contains its derivative, can be carried out as follows: 67 g quercetin dihydrate are evenly stirred into 280 g distilled water, which was previously heated to about 60 ° C. The mixture (total amount 347 g) is constantly stirred for about five minutes using a magnetic stirrer while maintaining the temperature at 60 ° C. While continuing the stirring process, 653 g of polysorbate 80 are added and the temperature is increased to about 100 ° C.

The stirring process is continued at this temperature until the mixture (Total amount 1 kg) has become clear and transparent. This clear mix contains the incorporated 67 g quercetin.

1 g of this clear, stable, aqueous concentrate contains 67 mg quercetin. This For example, the quantity can be incorporated into a liter of finished beverage.

The stability of the concentrate according to the invention in its clarity and Water solubility is also given when the invention Concentrate or an agent made from it (e.g. beverage) gastric acid is added. This also applies if the concentrate z. B. to about 100 ° C. is heated. The concentrate is therefore characterized by excellent stability out.

Claims (17)

1. Active ingredient concentrate, which is a polysorbate and one or more of the Active ingredients genistein, daidcein, glycitein and / or their glycosides, quercetin or contains its derivatives. 2. Concentrate according to claim 1 without quercetin, characterized in that Weight ratio of genistein, daidcein, and glycitein and / or their Glycosides to polysorbate is about 1: 5. 3. Concentrate according to claim 1 or 2, characterized in that it is anhydrous is. 4. Concentrate according to one of the preceding claims without quercetin, the Active substances in a quantitative ratio of genistin, daidcin and glycitin and their glycosides of about 1.3: 1.0: 0.3 are present. 5. Concentrate according to one of the preceding claims, which contains polysorbate 20 and / or polysorbate 40 and / or polysorbate 60 and / or polysorbate 65 and / or polysorbate 80 . 6. Concentrate according to one of the preceding claims, characterized in that that after adding stomach acid and / or after heating to approx. 100 ° C remains stable in its clarity and water solubility. 7. Food containing an isoflavone and / or quercetin concentrate any of the preceding claims. 8. Personal care products containing an isoflavone and / or quercetin concentrate according to one of the preceding claims.   9. Cosmetic containing an isoflavone and / or quercetin concentrate any of the preceding claims. 10. Agent according to one of claims 7 to 9, the per kg about 0.01 g to about 1000 g of a concentrate of isoflavone and / or quercetin according to one of the contains the preceding claims. 11. A composition according to claim 10, characterized in that a concentrate after one of claims 1-5 is in encapsulated form. 12. A method for producing an active ingredient concentrate according to one of the Claims 1-5, characterized in that one or in heated polysorbate several genistein type isoflavones and the warm one Mixture is stirred evenly for about half an hour until a clear sediment-free concentrate results. 13. The method according to claim 12, characterized in that approximately 5 Parts by weight of polysorbate about one part by weight of a vegetable, isoflavones of the extract containing genistein type is entered. 14. A method for producing a concentrate according to any one of claims 1-5, characterized in that genistein, and / or daidcein and / or glycitein and / or quercetin or its derivatives stirred into heated water be, the water heated to about 100 ° C polysorbate with stirring is added and stirring is continued until a clear Concentrate results. 15. The method according to claim 14 for the production of a genistein and / or daidcein and / or glycitein-containing concentrate, characterized in that 40.0 g of this isoflavones or 100 g soybean extract is stirred into water at about 60 ° C. and the Stirring is continued for a predetermined period of time, for example ten minutes, then the temperature of the mixture is raised to 100 ° C. and 500 g of polysorbate 80 are stirred in. 16. A process for the preparation of a concentrate containing quercetin, characterized in that 67 g of quercetin dihydrate are sprinkled in 280 g of water at 60 ° C, stirring is continued for a predetermined period of about five minutes, the temperature of the water to 100 ° C increased and 653 g of polysorbate 80 is added. 17. The method according to any one of claims 12-16 for producing a Concentrate according to claim 1, characterized in that the genistein, and / or daidcein and / or glycitin and / or quercitin or its derivatives first added polysorbate and then water to this composition is added.