DD280037A5 - METHOD FOR PRODUCING ANIMAL SUBSTANCES, PREFERABLY SUITABLE FOR THE TREATMENT OF MASTITIS - Google Patents

Abstract

The invention relates to a method for the production of veterinary products, which are particularly suitable for the treatment of mastitis. According to the invention, the active ingredient is soft propolis in an amount of 0.5 to 10% by volume (prepared by cold process) and one or two low HLB emulsifiers (3-8) in an amount of 1 in each case to 6% by volume, in a given case, a high HLB emulsifier (8-18), and further comprising one or more additives which in practice consist of the commercial ointment or emulsion base materials, the viscosity-influencing adjuvants Lubricants, inert hydrophilic solvents and p H-regulating additives are selected.

Description

Characteristic of the known state of the art

Mastitis is according to Hungarian and international literature known to be caused by many factors (polyetiological) disease, so that no prevention or treatment with general efficacy is possible.

In most cases, mastitis affects cows with high milk yield, which is understandable given the fact that 300 to 500 liters of blood must flow through the mammary gland to produce 1 liter of milk. The new method is of great importance because, according to various authors, mastitis causes greater economic loss than all other parasitic and infectious diseases combined.

This statement is confirmed by the loss information.

The annual loss in Hungary currently amounts to ⁷ to 750 million forints, in the USA it amounts to 1294 million dollars, which is equivalent to 150 to 300 US dollars per cow per year. The mastitis-induced annual loss in European countries will be like ic! _t '. valued:

France-5000 million francs; United Kingdom-£ 35 million; GDR 170 million marks; FRG 450 million DM.

Milk gland inflammation generally reduces total milk production by 11% in each country.

The damages are listed below:

- The milk yield drops by 10 to 20%.

- The quality of milk deteriorates.

- The treatment costs are quite high.

- After the treatment, the milk must be eliminated as long as the active ingredients are not out of the organism, which in most cases takes 3 to 7 days.

- The value of livestock breeding is reduced to that of cattle breeding.

- Forced slaughter and dying are more common.

- Cows with lactitis must be secreted.

- The period of milk production is getting shorter.

- The risk of infection from healthy animals is great.

- People can also be harmed.

The incidence and incidence of mammary gland infections are greatly affected by the prevailing conditions of animal husbandry, feeding and hygiene as well as by the animal breeder himself. This means that the incidence and severity of molt inflammation are very dependent on dairy farming farms and individuals, depending on the above conditions. The infection causing pathogens (bacteria, fungi, viruses, etc.) reach the udder's milk tracts in most cases by the galactic pathway or via the udder's parenchyma, thus solving the disease with varying severity depending on its pathogenicity and organism resistance or the udder.

Local medical treatment is generally used when the disease is manifested by clinical symptoms. The previously known Hungarian as well as foreign preparations contain some strong antibiotics or combinations of antibiotics and sulfonamide. When using these preparations, depending on the type of preparation, a waiting period of 3 to 7 days or more is necessary, until the diseased udder or udder section returns milk suitable for human consumption.

Object of the invention

The invention aims to provide a method for the preparation of veterinary preparations for the treatment of mastitis without the above-mentioned disadvantage.

Explanation of the essence of the invention

It has been recognized that the preparations with which propolis as active ingredient, namely udder infusion, emulsion and ointment, in the prevention and treatment of the disease at least guarantee the same result as preparations containing antibiotics or sulfonamide, but without the above Disadvantage.

From the preparation of the special emulsifiers had to be determined, which allow the production of a stable preparation with the correct quality, and it had a seemingly "passive emulsion system" worked out, which is activated when hitting din milk in the diseased udder section natural emulsifiers of the milk as well as the conditions of absorption capacity of the o / v emulsifier due to the reduced fat content of the infected milk.

We have found that the best way to achieve our goal is to use two emulsifiers with low HLB (3 to 8) and V / O with 1-6% by volume in an ointment emulsion Base material with ideally selected ratio (eg white vaseline - liquid paraffin) to dissolve in a water bath. In Jor practice we use two emulsifiers; Lanalcolum (2VoI .-%) and Alkoholcetylstearylicus (1-2 vol .-%) are the most advantageous. On the edge surface of the phases, the two emulsifiers form a stable, dense film. The above raw material emulsifier system is sufficient for emulsifying soft propolis active agent of low viscosity sour cream substance, quite hydrophilic type and in an amount of 0.5-10 vol%.

Taking into account that the udder infusion of two deciliters meets several liters of milk in the milk gels of both dairy cows and non-dairy cows, the amount of soft propolis in an amount of 1-6 vol.%, Preferably 3 -4Vol .-%, and with high HLB values (8-18) with the emulsifier of the V / O type, best with sorboxetene derivative (polysorbate 20) mix, and so emulsify it in the above Gru idstoff, the emulsifier or contains a pair of emulsifiers of the V / O type. The system with complex emulsifier thus obtained is to be introduced from the disposable plastic container into the diseased udder section, where it meets the milk and is activated.

Under the influence of 0, 'V emulsifier (Sorboxetenderivat or polysorbate 20) and the present in slight excess in the milk complex Mischphesphatid-albumin complex as an emulsifier type O / V, further under the influence of the movement of the cow and the intensity of the biological movement of the udder occurs at Eutertemperatur to a phase reversal and the uninterrupted, sufficient release of active ingredient from dam dispersed in this way base material is guaranteed. All this is definitely proven by clinical results. The choice of emulsifiers used and their ratio are significantly influenced by the fundamental finding that the fat content of the milk of a cow with mastitis does not reach the average, and in this case also the excess capacity of bioionic emulsifier (mixed phosphatide-albumin complex) can be taken into account , The presence of Polyso.bat 20 in the capacity as O / V emulsifier is for the emulsification of the liquid propolis extract, for the stabilization of the system, i. H. to prepare the udder infusion, nicely unnecessary. However, when it hits the milk, it plays an essential role by being activated at that time.

Only a negligible amount of liquid paraffin (0 to 2% by volume) will separate from the viscous or thin ointment-like emulsion under special storage conditions - at extreme temperature intervals - when aerosol is added to the preparation.

Consequently, the most important feature of the preparation according to the invention is that its active ingredient is soft propolis (prepared by cold extraction) and contained in an amount of 0.5-10% by volume, and furthermore one or two emulsifiers with low HLB value (3-8) each having 1-6 vol%, in a given case, a high HLB emulsifier (8-18) and further containing one or more additives selected in practice from the following common ointment or emulsion bases, viscosity modifiers and lubricants, inert hydrophilic solvents and pH adjusting additives.

embodiments

The details of the invention can be demonstrated by the following examples:

example 1

Udder infusion of propolis for the treatment or prevention of acute, subacute and chronic bovine mastitis The following substances have been used for udder infusion:

Standard propolis concentrate (soft propolis) 15.0 g Polyoxaethene 400 30.0 g Lanalcolum 15.0 g AIc. cetylstearyl. 7.5 g Vaseline album 227,5g Polysorbatum 24.0 g Paraffinum liquidum 431,0g

The ingredients listed above give an udder infusion with a propolis content of 2% by volume.

For udder infusion with a propolis of 6% by volume, the following ingredients were used:

Standard propolis concentrate (soft propolis) 45, Og Lanalcolum 15, Og AIc. cetylstearyl. 7.5 g Vaseline album 227.5 g Polysorbatum 24, Og Paraffinum liquidum 431, Og

The total mass of the components used in both cases is 750.0 g.

Lanalcolum, Alcohol cetylstearylicus and Vaselin album were melted with stirring in a water bath followed by sterilization at 140 ° C for three hours and stirring under aseptic conditions until cooling. The homogenization took place after 24 hours. In the ointment base thus obtained polysorbate was emulsified under aseptic conditions, then propolis and liquid paraffin were added in portions with intensive stirring and also sterilized at 140 ° C for 3 hours, followed by homogenization. Dio viscous emulsion was filled with stirring and weighing into the container of the disposable plastic syringe and sealed with a flask. After sealing, the individual units were packed. The plastic syringe was cleaned and sterilized under aseptic conditions according to the storage of medical preparations.

Example 2

Salve emulsion with propolis content

Composition:

soft propolis 10 g

Propylene glycol 20 g

Lanalcolum 50 g

AIc. cetylstearyl. 30 g

Vaseline album 640 g

Paraffinum liquidum 200 g

Cl.ricini 50 g

Example 3 Composition:

Standard piopolis concentrate (soft propolis) 15 g

Lanalcol 25 g

Vaseline album 265 g

Polysorbate 24 g

Paraffinum liquidum 421 g

Example 4 Composition:

Standard propolis concentrate (soft propolis) 15 g

AIc. cetylstearyl. 25 g

Vaseline album 265 g

Polysorbate 24 g

Paraffinum liquidum 421 g

manufacturing

0.5-10% by volume (preferably 6% by volume) of soft propolis was mixed with an indifferent solvent of hydrophilic character (propylene glycol - Polioxaeten 400), for prophetic purposes with propylene glycol.

In the mixture of Vaseline album and liquid paraffin with 5-30VoI .-% (for practical purposes, 20 vol .-%) were one or two hydrocarbon-soluble emulsifiers with low HLB value (3-8) in an amount of 2-6 vol .-% dissolved in a water bath. It became a pair of emulsifiers that matched perfectly and formed a dense, homogeneous film, ζ. Β.

Lanalcol and AIc. cetylstearyl. used in a ratio of 5: 3 vol .-%. This ointment base was stirred until cool, homogenized the next day, and the homogeneous mixture of soft propolis and propylene glycol was emulsified therein in small portions.

The prepared preparations according to the invention (udder infusion, ointment and the like) have numerous advantages in terms of efficacy and cost-effectiveness in comparison to the preparations containing customary active ingredients customary hitherto.

The advantages are the following:

The active substance content is to a large extent bactericidal, viricidal and fungicidal.

- There is no food-hygiene waiting period, as the active substance is a natural substance.

- The preparations have a significant anti-inflammatory, local anesthetic, epidermizing and analgesic effect.

- After use of the preparations, the milk originating from the treated udder no longer has a content of germicidal substance after 6 hours.

- Therefore, the value of the milk does not fall.

The use of the preparation has clearly demonstrated the above advantages

 Of course, the usual basic principles of application should be observed:

- Medical treatment should begin at the earliest stage of the disease.

- The treatment must be carried out professionally (lege artis).

- The right treatment period is required (until the symptoms disappear or longer).

Taking all this into account, the effectiveness of the preparation is limited to ten dairy farmers: Farms with different performance and production levels have been tested, and the results have been compared with those of previously used preparations containing 3-D antibiotics.

The following experiences were made:

All of the dairy farms involved in the experiment have consistently applied the preparation to peracute, acute, subacute, and chronic mastitis, which was evident at each lactation stage. A farm had information on an earlier investigation of the pathogens of the milk fever, expressed as a percentage of occurrence, as follows:

E. coli 18.9% St. Uberis 13.9% Str. Dysgalactiae 12.8% St. agaiactiae 2.1% Staph. aureus 7.5% Staph. albus 4.3% Corynebact. pyogenes 7.5% Klebsiella 4.8% coliform bact. 4.3% Bac. subtilis 3.2% Pseudo aeruginosa 1.6% Bac. cereus 1.1% Bac. negativum 0.5% gemmiparous fungi 4.8% 100.0%

After scattered bacteriological results of the other farms specializing in dairy cattle breeding similar pathogens could be found in cases of mammary inflammation.

- In 32 cases, the preparation was used with signs of inflammation observed in an udder part (pain, redness of the udder skin, slight fever), but no visible change in the milk could be observed. All udders with one exception - have recovered after a treatment. In 14 cases, the recovery was after 2 treatments without any pathological change in the milk. In 15 cases, flocculation and pus were observed mainly in the first milk jets. In these cases treatment was continued. The condition of the milk and the udder normalized in 8 cases after the third treatment, in 7 cases after the fourth treatment, and in 3 cases not only purulent flakes were found but also a change in the consistency of the milk, in that they were thinner and became whey-like. The recovery took place in 2 cases after the fifth treatment and in one case after the sixth treatment. The treatments were done every 24 hours. Although previous use of antibiotic-containing udder infusion preparations - mainly imported products - in cases similar to those described above, had shown complete recovery after as many treatments, the milk had to be discarded for 3-7 days or more.

- In 25 cases, mammary inflammation was observed in one stage, as changes in the milk, mainly in the first milk jets, namely flocculation, lumps to pus formation showed. Pain and redness in the udder sections that produce a change in the consistency of the milk have not been manifested explicitly. Other common symptoms, such as mild fever and poor appetite, were observed in 6 animals. The other animals, with the exception of the 6 animals mentioned above, recovered after three to four treatments. Full recovery was seen in 4 animals after 5 treatments and in 1 animal after 6 treatments. In one animal, there was a greater change in milk despite treatment. The milk became a kind of whey and turned red and had to be dried. The udder part became unsuitable for milk secretion.

When using the previously used antibiotic-containing udder infusions, a recovery of 50% was observed in the above cases only if in addition to the local intramammal treatment also antibiotics and electrolytes were used parenterally.

- In 12 cases, the product was used in animals that showed very severe general symptoms (high fever, myasthenia, severe diarrhea).

More than one udder section was affected by the molt inflammation, acute clinical symptoms and a serious change in milk were observed. The milk was definitely purulent, yellow in appearance, whey-like. In 6 cases, antibiotics, electrolytes and hepatoprotective therapy were used with the udder infusion. On the fifth day after treatment, 5 cows were fully recovered, general symptoms had disappeared, and milk consistency returned to normal. In a single case, half of the rear of the animal was completely paralyzed so that it had to be force-slaughtered. In four cases, as the myasthenia was not yet so clear, the udder therapy was applied twice daily without adjunctive adjunctive therapy. The udder regained its normal state on the fifth day, and on the seventh day no changes in milk were seen. In two cases, parenteral therapy with antibiotics and electrolytes was taken on the third day after treatment of the udder sections, as the general symptoms did not disappear. These symptoms disappeared on the seventh day after detection, and the milk returned to normal on the eighth day.

When using the previously used antibiotic-containing udder infusions, the additional parenteral therapy was also used in the above cases. Generally speaking, 80% of the cases recovered, 20% died or had to be slaughtered or discarded because several udder sections had changed permanently.

In a summary of the clinical experience of the use of the propolis-containing udder infusion prepared according to the invention, the following statement can be made:

- It has been proven to all users that the milk produced by the treated udder section was ready for industrial use at full price as early as 6 hours after treatment of the udder. This means that the milk produced by the treated udder section need not be destroyed over 3 to 7 days after treatment (which is invaluable to the economy).

The effectiveness of the preparation is the same as (in some cases even better than) the antibiotic-containing preparations available in Hungary as domestic or foreign products. .

It is also of great importance to the economy that the product itself can be produced in Hungary and that only a small amount of basic materials has to be imported. This allows the saving of foreign exchange on a large scale. Both the producer price and the wholesale price are cheaper than the price of imported products, which also means savings for the producer.

Claims (1)

Procedures for the preparation of veterinary products, which are particularly suitable for the treatment of mastitis, characterized in that the active ingredient comprises soft propolis in an amount of 0.5 to 10% by volume (prepared by cold process) and one or two emulsifiers of low HLB Value (3-8) in an amount of 1 to 6 vol.% Each, in a given case a high HLB emulsifier (8-18), and further one or more additives are included in the practice be selected from the common Saiben- or emulsion bases, the viscosity-influencing excipients, lubricants, inert hydrophilic solvents and pH-regulating additives. Field of application of the invention The invention relates to a process for the preparation of veterinary products, for. As udder infusion, ointment, emulsion, etc., which are particularly suitable for the treatment of mastitis.