DD264705A1 - CAPILLARY MULTI-POINT IMPFVORRICHTUNG - Google Patents

Abstract

The invention relates to a capillary multipoint vaccine device. Field of application of the invention is bacteriology. The capillary multipoint vaccination device is used for the simultaneous testing of several bacterial stoppers for their properties. According to the invention, the object is achieved by a vaccine device consisting of a lower and upper part. In the upper part, glass capillaries are movably suspended for receiving the germinal suspensions, which are lowered en bloc onto a nutrient medium surface, wherein the contact pressure depends only on the weight of the capillaries and holding elements, so that a defined volume of liquid escapes through contact of the capillaries with the surface of the nutrient medium and damage to the Nährmedienoberflaeche, contamination and the access of atmospheric oxygen are largely avoided.

Description

The advantages of the invention are primarily in the ease of use. The use of disposable capillaries eliminates the need for regeneration. Due to the free suspension of the capillaries damage to the Nährmedienoberflnche be avoided. The access of Luftsaiierstoff and contamination by the surrounding atmosphere are largely excluded.

embodiment

The invention will be explained in more detail using the example of a capillary multipoint vaccination device. In the zugenörigen drawings is in

Figure 1 shows the capillary multipoint vaccination device with the template block and in Figure 2 oine associated assembly theory.

Dio capillary multipoint inoculation device consists of a lower part, which stands on the work surface, and an upper part, which is arranged on 4 vertical rods movable above the lower part and can be lowered manually against the resistance of two springs. The lower part contains recesses for the template block or for a Petri dish. The horizontal and lateral plates of both parts are made of transparent material (for example Piacryl). In the horizontal plates of the upper part of the KapillarMultipoint-lmpfvorrichtung are vertically stacked holes for the movable recording of 31 glass capillaries. The disposable capillaries, which are commonly used for capillary blood collection for acid-base balance studies, have an outer diameter of 1.8 μm. Due to the arrangement of the capillaries, distances of the spots of at least 14 mm and of the petri dish margin of at least 8 mm were obtained on a round culture medium surface (Petri dish - diameter 30 mm). The capillaries are held by rubber discs over the top plate of the top of the capillary multipoint vaccinator, so placing on the nutrient media surface is only at the net weight of the capillaries and rubber discs. To equip the capillary multipoint inoculation device with the stenilized glass capillaries is a Bes'.ückungslehre in the first inserted the rubber discs and then the capillaries through the holes of the inverted upper part of the copilar-multipoint vaccination device are fed through.

The filling of the capillaries with the prepared microbial suspensions is carried out by lowering the upper part of the capillary N / Iultipoint inoculation device with the capillaries in the filled template block, wherein the liquid rises by adhesion forces about 20mm in the capillaries.

For inoculation, the capillary multipoint vaccination device is now placed on a nutrient media plate and lowered the top; each time the glass capillaries touch the culture media surface, a small amount of fluid exits. The inoculums are about 0.8 μΙ in the case of soaked capillaries and about 0.3 μΙ after 25 to 30 inoculations.

If additional nutrient media plates are to be inoculated, the capillaries must be refilled in the manner described.

Claims (2)

Capillary multipoint vaccination device consisting of a lower part with recesses for receiving a template block or a Nährmedienschale and connected to this lowerable upper part, characterized in that in the upper part glass capillaries are suspended with holding elements movable, the loading of the capillaries by lowering de 'upper part and Plunging them into a template block, for inoculating a nutrient medium plate, the top of the capillary multipoint vaccine is lowered over this, the contact pressure of the capillaries on the Nährmedienoberfläche only the weight of the capillaries and the corresponding holding elements and each time the Nährmedienoberfläche a defined amount of liquid exit. For this 2 pages drawings Field of application of the invention The field of application of the invention is bacteriology. The capillary multipoint vaccination device is used for the simultaneous examination of several bacterial strains for their sensitivity especially to chemotherapeutic agents as well as their biochemical or other properties; it is preferably used for larger numbers of inoculating culture media plates. Characteristic of the known technical solutions Multipoint inoculators Z-mimic devices (replicators) are in de; microbiological laboratory practice in use. There is a multi-syringe applicator is known, which is equipped with syringes and needles, whereby the syringe tempol are lowered evenly by a certain distance by a plate with advancing device, so that droplets of defined volumes on the nutrient media surface can reach from the cannulas (ZIERDT et al , 1960, manufacturer: Johnny Brown Machine Shop, Northport, Al, USA). This solution is relatively cumbersome to handle by the need for sterilization of the syringes and cannulas or the high cost of using disposable disposable materials, the manner of loading the syringes with germ suspensions and the assembly of the replicator, which is only in oiner large numbers to be inoculated Nährmedienplatten profitable isi. Due to the rigid attachment of the syringes, damage to the nutrient media surface during lowering can not be ruled out or can only be avoided by greater precision in operation. Object of the invention The aim of the invention is to reduce the expense of equipping a vaccination device using simple and known technical means, to ensure easy handling and to preclude any violation of the nutrient media surface and the access of atmospheric oxygen. Explanation of the essence of the invention The invention has for its object to provide a multipoint jmpfvorrichtung dif / can be equipped and charged with little effort and ensures multiple inoculation of culture media plates while avoiding contamination or the access of atmospheric oxygen. According to the invention the object is achieved in that in the capillary-multipoint vaccination device, which consists of a lower and a top, glass capillaries for receiving the microbial suspensions are movably suspended in such a way that they en with the top of Kapillnr-Multlpoint vaccination device by pressure bloc can be lowered to an underlying Nährmedienoberfläche. The lower part of the capillary multipoint vaccination device stands on a work surface and contains a recess for the template block or for a culture medium dish. On vertical rods, the upper part of the capillary-multipoint vaccination device is movably arranged, whereby the rest position is ensured in the upper position with stop by springs. The top of the capillary multipoint vaccination device contains holes for receiving the glass capillaries, which are thus displaced in the vertical direction. To prevent capillary leakage, they are held by retaining members over the top plate of the capillary-mammipoint seedling top. The capillaries are loaded with the seed suspensions by lowering the upper part of the capillary multipoint inoculation device with the capillaries into the filled template block; Adhesion forces increase the fluid in the capillaries. For inoculation, the top of the capillary multipoint vaccination device mk is now lowered onto the nutrient media surface of the capillaries. The Aufsetzdruck on the Nährmedienoburf laugh depends only on the weight of the glass capillaries and the associated holding elements. When touching the Glaska'pillars with the nutrient media surface, a defined amount of liquid flows out.