DD228562A1 - METHOD OF CULTURING BORDETELLA BRONCHISEPTICA - Google Patents
METHOD OF CULTURING BORDETELLA BRONCHISEPTICA Download PDFInfo
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- DD228562A1 DD228562A1 DD26957984A DD26957984A DD228562A1 DD 228562 A1 DD228562 A1 DD 228562A1 DD 26957984 A DD26957984 A DD 26957984A DD 26957984 A DD26957984 A DD 26957984A DD 228562 A1 DD228562 A1 DD 228562A1
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- German Democratic Republic
- Prior art keywords
- bordetella bronchiseptica
- glutamic acid
- culture medium
- cultivation
- yield
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Das Ziel der Erfindung besteht darin, Bordetella bronchiseptica in aminosaeurehaltige Naehrmedien unter aeroben Bedingungen und Zusatz von Glutaminsaeure in einer Zuechtungsapparatur zu kultivieren und die Ausbeute zu erhoehen. Das erfindungsgemaesse Verfahren ist dadurch gekennzeichnet, dass 5-40 g/l Glutaminsaeure dem Kulturmedium zugefuehrt wird.The aim of the invention is to cultivate Bordetella bronchiseptica in aminosa-containing nutrient media under aerobic conditions and addition of glutamic acid in a growth apparatus and to increase the yield. The process according to the invention is characterized in that 5-40 g / l glutamic acid is added to the culture medium.
Description
Verfahren zur Kultivierung von Bordetella bronchisepticaMethod of cultivating Bordetella bronchiseptica
Die Erfindung betrifft ein Verfahren zur Kultivierung von Bordetella bronchiseptica unter aeroben Bedingungen in aminosäurehaltigen wäßrigen Kulturmedien·The invention relates to a process for the cultivation of Bordetella bronchiseptica under aerobic conditions in amino acid-containing aqueous culture media.
Die bekannten Verfahren zur Züchtung von Bordetella bronchiseptica beruhen auf der aeroben Kultivation in kohlehydratfreien Kaseinhydrolysat-Uährböden (Amer. J. publ. HLth. ^6 (1946) 371).The known processes for the cultivation of Bordetella bronchiseptica are based on the aerobic cultivation in carbohydrate-free casein hydrolyzate agar plates (Amer. J. publ. HLth. ^ 6 (1946) 371).
Die Kultivationsverfahren sind durch eine geringe Ausbeute an Bordetella bronchiseptica gekennzeichnet.The cultivation methods are characterized by a low yield of Bordetella bronchiseptica.
Sie liegt zwischen 80-1001(K Keime/ml.It is between 80-1001 (K germs / ml.
Das Ziel der Erfindung besteht darin, die Ausbeute der aeroben Kultivation von Bordetella bronchiseptica in aminosäurehaltigen wäßrigen Kulturmedien erheblich zu steigern.The aim of the invention is to significantly increase the yield of aerobic cultivation of Bordetella bronchiseptica in amino acid-containing aqueous culture media.
Das erfindungsgemäße Verfahren zur !Cultivation von Bordetella bronchiseptica unter aeroben Bedingungen in amino— säurehaltigen wäßrigen Kulturmedien ist dadurch gekennzeichnet, daß zu den handelsüblichen. Kaseinhydrolysaten Glutaminsäure zugesetzt wird·The inventive method for! Cultivation of Bordetella bronchiseptica under aerobic conditions in amino acid-containing aqueous culture media is characterized in that the commercial. Casein hydrolysates glutamic acid is added ·
Dem wäßrigen aminosäurehaltigeη Kulturmedium werden bevorzugt zwischen 5 und 40 g Glutaminsäure/1 zugesetzt. Der' Hauptvorteil des mit Glutaminsäure angereicherten Nährbodens besteht in der wesentlichen Steigerung der Ausbeute von Bordetella bronchiseptica bei der aeroben Kultivatioh. Sie liegt zwischen 180 und 200·10^ Keime/ml.Preferably, between 5 and 40 g of glutamic acid / l are added to the aqueous amino acid-containing culture medium. The main advantage of the glutamic acid-enriched medium is the substantial increase in the yield of Bordetella bronchiseptica in the aerobic culture. It is between 180 and 200 · 10 ^ germs / ml.
Das erfindungsgemäße Verfahren soll an der aeroben Kultivation von Bordetella bronchiseptica in einem 60 1 Blasensäulenfermentor erläutert werden. Der Blasensäulenfermentor wird mit 60 1 Kulturmedium folgender Zusammensetzung unter sterilen Bedingungen gefüllt und mit 100 ml Saatkultur beimpft.The method according to the invention will be explained on the aerobic cultivation of Bordetella bronchiseptica in a 60 1 bubble column fermentor. The bubble column fermentor is filled with 60 1 culture medium of the following composition under sterile conditions and inoculated with 100 ml of seed culture.
Zusammensetzung des Kulturmediums: Kaseinhydrolysat 10 g/l MgSO4 · TH2O 0,33 g/l KH2PO4 0,83 g/l Composition of the culture medium : Casein hydrolyzate 10 g / l MgSO 4 .TH 2 O 0.33 g / l KH 2 PO 4 0.83 g / l
Cystein . 0,5 g/lCysteine. 0.5 g / l
Nach dem Erreichen einer Dichte von 100*10^ Keime an Bordetella bronchiseptica beginnt das Wachstum der Keime zu stagnieren· Nach der Zugabe von 10 g Glutaminsäure/1 Kulturmedium steigt die Ausbeute an Bordetella bronchiseptica auf 180-200·10^ Keime.After reaching a density of 100 * 10 ^ germs on Bordetella bronchiseptica, the growth of the germs begins to stagnate · After the addition of 10 g of glutamic acid / 1 culture medium, the yield of Bordetella bronchiseptica increases to 180-200 · 10 ^ germs.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD26957984A DD228562A1 (en) | 1984-11-19 | 1984-11-19 | METHOD OF CULTURING BORDETELLA BRONCHISEPTICA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD26957984A DD228562A1 (en) | 1984-11-19 | 1984-11-19 | METHOD OF CULTURING BORDETELLA BRONCHISEPTICA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD228562A1 true DD228562A1 (en) | 1985-10-16 |
Family
ID=5562318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD26957984A DD228562A1 (en) | 1984-11-19 | 1984-11-19 | METHOD OF CULTURING BORDETELLA BRONCHISEPTICA |
Country Status (1)
| Country | Link |
|---|---|
| DD (1) | DD228562A1 (en) |
-
1984
- 1984-11-19 DD DD26957984A patent/DD228562A1/en unknown
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