DD204265A5 - Verfahren zur herstellung eines rekombinanten dna klonierungsvektors - Google Patents
Verfahren zur herstellung eines rekombinanten dna klonierungsvektors Download PDFInfo
- Publication number
- DD204265A5 DD204265A5 DD82244037A DD24403782A DD204265A5 DD 204265 A5 DD204265 A5 DD 204265A5 DD 82244037 A DD82244037 A DD 82244037A DD 24403782 A DD24403782 A DD 24403782A DD 204265 A5 DD204265 A5 DD 204265A5
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- plasmid
- bam
- restriction fragment
- plasmids
- dna
- Prior art date
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- HUKYPYXOBINMND-HYUJHOPRSA-N methymycin Chemical compound C[C@H]1C[C@@H](C)C(=O)\C=C\[C@@](O)(C)[C@@H](CC)OC(=O)[C@H](C)[C@H]1O[C@H]1[C@H](O)[C@@H](N(C)C)C[C@@H](C)O1 HUKYPYXOBINMND-HYUJHOPRSA-N 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- OXFYAOOMMKGGAI-JLTOUBQASA-N narbomycin Chemical compound C[C@H]1C[C@@H](C)C(=O)\C=C\[C@@H](C)[C@@H](CC)OC(=O)[C@H](C)C(=O)[C@H](C)[C@H]1O[C@H]1[C@H](O)[C@@H](N(C)C)C[C@@H](C)O1 OXFYAOOMMKGGAI-JLTOUBQASA-N 0.000 description 1
- OXFYAOOMMKGGAI-UHFFFAOYSA-N narbomycin Natural products CC1CC(C)C(=O)C=CC(C)C(CC)OC(=O)C(C)C(=O)C(C)C1OC1C(O)C(N(C)C)CC(C)O1 OXFYAOOMMKGGAI-UHFFFAOYSA-N 0.000 description 1
- 229950003438 neutramycin Drugs 0.000 description 1
- DANUORFCFTYTSZ-BIBFWWMMSA-N nigericin Chemical compound C([C@@H]1C[C@H]([C@H]([C@]2([C@@H](C[C@](C)(O2)C2O[C@@](C)(CC2)C2[C@H](CC(O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C)O1)C)OC)[C@H]1CC[C@H](C)C([C@@H](C)C(O)=O)O1 DANUORFCFTYTSZ-BIBFWWMMSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940066779 peptones Drugs 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- NSKGQURZWSPSBC-VVPCINPTSA-N ribostamycin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O[C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](N)C[C@@H]1N NSKGQURZWSPSBC-VVPCINPTSA-N 0.000 description 1
- 229960001548 salinomycin Drugs 0.000 description 1
- 235000019378 salinomycin Nutrition 0.000 description 1
- HJKXMQLJTKUBMG-INVUDJPSSA-N seldomycin Chemical compound N[C@H]1[C@@H](N)[C@H](OC)CO[C@@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)C[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N HJKXMQLJTKUBMG-INVUDJPSSA-N 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- ACTOXUHEUCPTEW-KWBWCIJSSA-N spiramycin I Chemical compound O([C@H]1/C=C/C=C/C[C@@H](C)OC(=O)C[C@@H](O)[C@@H]([C@H]([C@@H](CC=O)C[C@H]1C)O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1)N(C)C)O)OC)[C@H]1CC[C@H](N(C)C)[C@H](C)O1 ACTOXUHEUCPTEW-KWBWCIJSSA-N 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 101150020293 sty gene Proteins 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- JARYYMUOCXVXNK-IMTORBKUSA-N validamycin Chemical compound N([C@H]1C[C@@H]([C@H]([C@H](O)[C@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)CO)[C@H]1C=C(CO)[C@H](O)[C@H](O)[C@H]1O JARYYMUOCXVXNK-IMTORBKUSA-N 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-O vancomycin(1+) Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C([O-])=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)[NH2+]C)[C@H]1C[C@](C)([NH3+])[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-O 0.000 description 1
- 229950001272 viomycin Drugs 0.000 description 1
- GXFAIFRPOKBQRV-GHXCTMGLSA-N viomycin Chemical compound N1C(=O)\C(=C\NC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)C[C@@H](N)CCCN)CNC(=O)[C@@H]1[C@@H]1NC(=N)N[C@@H](O)C1 GXFAIFRPOKBQRV-GHXCTMGLSA-N 0.000 description 1
- 239000002132 β-lactam antibiotic Substances 0.000 description 1
- 229940124586 β-lactam antibiotics Drugs 0.000 description 1
- 150000003952 β-lactams Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31171181A | 1981-10-15 | 1981-10-15 | |
| US06/404,082 US4513086A (en) | 1981-10-15 | 1982-08-02 | Cloning vectors for use in streptomyces and related organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD204265A5 true DD204265A5 (de) | 1983-11-23 |
Family
ID=26978029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD82244037A DD204265A5 (de) | 1981-10-15 | 1982-10-15 | Verfahren zur herstellung eines rekombinanten dna klonierungsvektors |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US4513086A (OSRAM) |
| EP (1) | EP0077649A3 (OSRAM) |
| AU (1) | AU558007B2 (OSRAM) |
| CA (1) | CA1194434A (OSRAM) |
| DD (1) | DD204265A5 (OSRAM) |
| DK (1) | DK454882A (OSRAM) |
| GB (1) | GB2107716B (OSRAM) |
| GR (1) | GR77017B (OSRAM) |
| HU (1) | HU196239B (OSRAM) |
| IE (1) | IE54027B1 (OSRAM) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
| US4513085A (en) * | 1982-10-07 | 1985-04-23 | Eli Lilly And Company | Functionally independent cloning vectors for use in streptomyces |
| US4468462A (en) * | 1982-12-22 | 1984-08-28 | Eli Lilly And Company | Vectors for cloning in streptomyces |
| US4643975A (en) * | 1984-01-05 | 1987-02-17 | Eli Lilly And Company | Novel cloning vectors for use in streptomyces, escherichia coli and related organisms |
| US4666846A (en) * | 1984-02-17 | 1987-05-19 | Eli Lilly And Company | Novel cloning vectors containing selectable genetic markers for use in streptomyces and related organisms |
| EP0158872B1 (de) * | 1984-03-31 | 1989-01-18 | Hoechst Aktiengesellschaft | Das Streptomyceten-Plasmid pSG5, Verfahren zu seiner Gewinnung und seine Verwendung |
| DE3412093A1 (de) * | 1984-03-31 | 1985-10-10 | Hoechst Ag, 6230 Frankfurt | Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon |
| US4752577A (en) * | 1984-09-13 | 1988-06-21 | Eli Lilly And Company | Method for using a novel transcriptional- and translational-activating sequence in streptomyces |
| US4710464A (en) * | 1984-09-27 | 1987-12-01 | Eli Lilly And Company | Transcription terminators |
| US4745056A (en) * | 1984-10-23 | 1988-05-17 | Biotechnica International, Inc. | Streptomyces secretion vector |
| US4753880A (en) * | 1984-12-24 | 1988-06-28 | Eli Lilly And Company | Method of selection for spiramycin resistance in streptomyces |
| US4874748A (en) * | 1986-03-24 | 1989-10-17 | Abbott Laboratories | Cloning vectors for streptomyces and use thereof in macrolide antibiotic production |
| US5149639A (en) * | 1986-03-24 | 1992-09-22 | Abbott Laboratories | Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production |
| DK1618183T3 (en) * | 2003-04-29 | 2015-02-23 | Danisco Us Inc | New Bacillus cellulase 029cel |
| US7892807B2 (en) * | 2003-04-30 | 2011-02-22 | Danisco Us Inc. | Bacillus BagCel cellulase |
| JP4757191B2 (ja) | 2003-04-30 | 2011-08-24 | ジェネンコー・インターナショナル・インク | 新規なバチルスｍＨＫｃｅｌセルラーゼ |
| EP1625202A4 (en) * | 2003-05-12 | 2010-10-20 | Genencor Int | NEW LIPOLYTIC ENZYME LIP1 |
| WO2004101759A2 (en) * | 2003-05-12 | 2004-11-25 | Genencor International, Inc. | Novel lipolytic enzyme lip2 |
| EP1625217B1 (en) * | 2003-05-12 | 2014-12-17 | Danisco US Inc. | Novel lipolytic enzyme elip |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1979001169A1 (en) * | 1978-06-01 | 1979-12-27 | D Hopwood | Streptomyces plasmids and their use as vectors for the incorporation of nucleic acid into cellular systems |
| US4273875A (en) * | 1979-03-05 | 1981-06-16 | The Upjohn Company | Plasmid and process of isolating same |
| GB2048894A (en) * | 1979-05-11 | 1980-12-17 | Gist Brocades Nv | Plasmid |
| US4340674A (en) * | 1980-05-05 | 1982-07-20 | The Upjohn Company | Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces |
| US4332898A (en) * | 1980-05-22 | 1982-06-01 | The Upjohn Company | Hybrid plasmid and process of making same |
| US4332900A (en) * | 1980-10-01 | 1982-06-01 | The Upjohn Company | Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia |
| EP0058002A3 (en) * | 1981-01-26 | 1983-06-29 | The Upjohn Company | Process for stabilising potential plasmid vectors and novel plasmids |
| US4342829A (en) * | 1981-05-06 | 1982-08-03 | Eli Lilly And Company | Process for preparing narasin |
-
1982
- 1982-08-02 US US06/404,082 patent/US4513086A/en not_active Expired - Fee Related
- 1982-10-11 GR GR69493A patent/GR77017B/el unknown
- 1982-10-12 CA CA000413247A patent/CA1194434A/en not_active Expired
- 1982-10-14 AU AU89351/82A patent/AU558007B2/en not_active Ceased
- 1982-10-14 IE IE2488/82A patent/IE54027B1/en unknown
- 1982-10-14 HU HU823270A patent/HU196239B/hu not_active IP Right Cessation
- 1982-10-14 DK DK454882A patent/DK454882A/da not_active Application Discontinuation
- 1982-10-14 GB GB08229337A patent/GB2107716B/en not_active Expired
- 1982-10-14 EP EP82305471A patent/EP0077649A3/en not_active Ceased
- 1982-10-15 DD DD82244037A patent/DD204265A5/de unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0077649A2 (en) | 1983-04-27 |
| GB2107716B (en) | 1985-01-30 |
| DK454882A (da) | 1983-04-16 |
| AU8935182A (en) | 1983-04-21 |
| GB2107716A (en) | 1983-05-05 |
| EP0077649A3 (en) | 1983-07-27 |
| CA1194434A (en) | 1985-10-01 |
| IE54027B1 (en) | 1989-05-24 |
| IE822488L (en) | 1983-04-15 |
| GR77017B (OSRAM) | 1984-09-04 |
| AU558007B2 (en) | 1987-01-15 |
| HU196239B (en) | 1988-10-28 |
| US4513086A (en) | 1985-04-23 |
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