CZ306392B6 - Additional food for babies and children to increase oral tolerance of milk proteins - Google Patents

Abstract

In the present invention, there is disclosed a grain-milky or milky-grain additional food with optimized milk component consisting of goat milk and goat proteins with exclusion of cow proteins. The use thereof, starting from the end of the 4th month of baby life, brings the increase of oral tolerance of milk proteins. The milky component of the invented additional foods has a modified protein profile containing goat alpha-s1-cassein in the range of 10 to 200 mg of the alpha-s1-cassein in 100 ml of the administered additional food and at the same time, it complies with the parameters of the initial or continuing baby milk according to valid regulations. The optimal starting dosage of the alpha-s1-cassein allergen is 10 mg in 100 ml of the additional food from the end of the 4th month of baby life with subsequent increasing up to the value of 200 mg in 100 ml of the additional food from the end of the 8th month of baby life. In such a manner, it is possible to incite the increasing oral tolerance of the milk proteins through a controlled, natural method.

Description

Infant formulas and follow-on formulas to increase the oral tolerance of milk proteins

Field of technology

The present invention relates to cereal milk and milk cereal infant formulas and baby foods with an optimized milk component of infant infant formula and goat milk consisting of goat milk and goat proteins excluding cow proteins aimed at increasing the oral tolerance of milk proteins.

Prior art

The protein components of cow's milk are responsible for the vast majority of intolerances or allergies manifesting in infants, young children and, in some cases, persisting into adulthood. The incidence of cow protein allergy or intolerance varies between 0.36 and 7.5% of the population in different countries, depending on monitoring methods.

Symptoms caused by intolerance to cow's milk proteins are manifested in affected individuals in varying degrees, the most common being gastrointestinal disorders observed in 55 to 80%, skin reactions in 20 to 40% or respiratory symptoms in 5 to 20% of people with allergies or intolerance to cow proteins. .

At the end of the last century, so-called hypoallergenic baby foods using either heat-treated proteins or protein hydrolysates with varying degrees of degradation of protein molecules were developed and used as a preventive solution to the risk of developing symptoms due to allergy or intolerance of cow proteins. U.S. Pat. No. 5,436,020 A addresses the loss of 5 to 6% of non-proteinaceous nitrogenous nutrients in the removal or reduction of allergenic protein components by chemical, physical or enzymatic processes. The patent documents the reduction of bovine β-lactoglobulin and α-s casein using chemical agents such as sodium hydroxide, hydrochloric acid. The use of strong chemical agents increases the risk of damage to other biologically active components of milk. Patent CN 102144668 A is aimed at approximating the composition of goat's milk to breast milk and its essence is the addition of hydrolyzed lactalbumin peptides and other nutrients to pasteurized goat's milk. The patent does not address the issue of the content or reduction of the major allergen α-s casein. The essence of patent application CZ 1992-1220 A is the fractionation of goat's milk with subsequent separation or hydrolysis of lactoglobulins. The patent does not address the issue of α-s casein content.

However, even infant formulas based on protein hydrolysates do not provide 100% protection against the onset and development of cow protein allergy. In cases where a hypersensitive child is unable to receive normal hypoallergenic infant milk with cow's milk protein hydrolysates without difficulty, it is necessary to provide nutrition either with a preparation with extensively hydrolyzed cow's protein, or soy replacement food or even only through the amino acid complex. Soy protein-based infant formulas and follow-on formulas may not be a safe alternative, as the risk of an allergic reaction has been shown for this type of plant protein.

Despite massive long-term applications of infant formulas and follow-on formulas based on HA hydrolysates bearing the HA designation, the incidence of allergies or intolerances to this type of protein is not decreasing, but on the contrary is increasing.

From the point of view of world production, goat's milk is the second most frequently consumed milk. The chemical, physical and nutritional properties of goat's milk justify its use in the nutrition of infants and young children, provided that fortification or modeling of certain nutritional parameters is provided.

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Patent RU 2384069 C describes the composition and production of adapted goat's milk for infants, but regardless of the content of the allergen α-s casein, only with a focus on bringing the ratio of casein and whey proteins closer to 40:60, which is typical for breast milk. The patent is aimed at improving the nutritional value of the commercial preparation Nanny.

The main triggers of allergies and intolerances of cow's milk proteins are demonstrably both caseins - especially alpha-sl-casein, and a fraction of whey proteins called beta-lactoglobulin.

Numerous research studies have shown lower allergenicity of goat's milk and its higher oral tolerance as well as 10 for patients with a proven allergy to cow's protein. It has been shown that the protein component eliciting an immune response in the case of goat's milk is only the casein fraction referred to as alpha-s1-casein, the content of which is significantly lower than that of cow's milk. ElAgamy (The challenge of cow milk protein allergy, October 20, 2006) presents a comparison of the peptide composition of casein and whey fractions of milk of a selected group of mammals whose milk is also used in human nutrition. It deals with cross-reactions of cow's and goat's milk proteins.

It indicates the value of the therapeutic benefit of goat's milk at 60% compared to the risk of an allergic reaction to cow's milk.

No allergenicity has been demonstrated for goat's whey protein. The content of α120 fa-sl-casein in goat's milk is associated with a genotype polymorphism and ranges from 0.9 g to

4.1 g in 1 liter of milk, with low levels of alpha-s1-casein found in the Sanski, Alpine and Toggennburg breeds. High levels of alpha-sl-casein are characteristic of the Nubian and La Mancha breeds. Cow's milk contains between 5.9 and 17.5 g of alpha-s1-casein in 1 liter of milk. The question of the polymorphism of two Czech goat breeds in relation to the content of the allergen α-s casein 25 is described in the article by Sztankóová et al. (Czech J. Anim. Sci. 52 2007 (7): 199 - 202. It specifies the occurrence of allele species responsible for different levels of α-s production of casein of Czech shorthair breeds.The problem is solved from the point of view of breeding genetics without impact on human nutrition.

The essence of the invention

An improvement of the current state and thus a reduction in the number of manifested intolerances or allergies to cow's milk proteins is achieved by early initiation of infant formulas and optimized milk component of infant formula and goat milk consisting of goat milk and goat proteins excluding cow proteins according to the invention. in the period from the end of the 4th to the 7th month, resp. 12 months of age and gradual induction of oral tolerance to milk proteins.

The gradual introduction of feedingstuffs with goat's milk proteins during breastfeeding, which is a natural physiological way of feeding in infancy, seems ideal. Processed cereal-based milk or milk-based cereals with an optimized milk component of infant formulas and follow-on milk consisting of goat's milk and goat proteins excluding cow proteins according to the invention should also be given as first foods in children receiving HA - initial or follow-on milk formula with hydrolysed bovine proteins. An exception is 45 dinci, whose basic nutrition is based on extensively hydrolyzed proteins or mixtures of amino acids.

The highest efficacy for increasing the oral tolerance of milk proteins is shown in cereal-based milk or milk-based foods with an optimized milk component consisting of goat's milk and goat's 50 proteins excluding cow's proteins meeting infant formula or follow-on milk with a modified protein profile to goat alpha. -sl casein ranged from 10 mg to 200 mg of alpha-s1-casein in 100 ml of administered foods and allowed a gradual increase in allergen intake depending on the maturation of the digestive enzyme system as the age of the child.

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Based on the determination of the alpha-s1-casein content in goat's milk, the optimal composition of the final milk component of the feed is achieved by modeling the protein profile so that the values of total protein content required by baby food legislation are achieved by the addition of goat whey protein. At the same time, contamination of all used recipes with cow's proteins must be avoided. The cereal-based milk or milk-based cereal-based foods based on goat infant formula or follow-on milk according to the patent contain a milk component whose proteins have not been modified or altered by any chemical, physical or enzymatic process. By controlled dosing of the declared allergen content in the supplement, its intake can be increased according to the age of the child and the degree of maturation of his digestive tract. The optimal starting dose of the alpha-sl-casein allergen is 10 mg in 100 ml of food from the end of the 4th month of life with a subsequent increase up to 200 mg in 100 ml of food from the end of the 8th month of life. This makes it possible to induce increasing oral tolerance of milk proteins in a controlled, natural way.

The resulting content of vitamins, minerals and trace elements shall be adjusted to the values laid down by the legislation in force for infant formulas and follow-on formulas in that country. Advantageously, cereal-based milk or milk-based foods with optimized goat's milk based on goat's milk and goat proteins can be enriched with prebiotics, probiotic microorganisms, nucleotides, polyunsaturated fatty acids, carbohydrates and other nutritionally important components, either individually or in mixtures.

Processed cereal-based or lactated cereal-based foods with optimized goat's milk and goat's protein may be produced in the form of instant powder mixtures which are prepared for consumption by the addition of water or as finished pasteurized or otherwise stabilized ready-to-use foods.

Examples of embodiments of the invention

Example 1

Rice cereal milk supplement for children from the end of the 4th month of life in the form of instant powder

Component g per 100 g of powder g per 100 ml of feed -18.7 g of powder + 90 ml of water

instant rice flour 45.0 8.4 initial milk nutrition (based on goat 's milk excluding cow protein) 38.0 7.1 carbohydrates (lactose, dextrose) 10.0 1.9 prebiotic fiber 2.0 0.4 lyophilized probiotic microorganisms 0.3 0.06

other components:

vitamins, minerals, trace elements, unsaturated fatty acids in the amounts recommended by the current directive for infant formulas and follow-on formulas protein profile: total protein content alpha-sl-casein content

12.5 2.3

53.5 mg 10 mg

Example 2

Cereal milk oatmeal for children from the end of the 5th month of life in the form of instant powder

Component g per 100 g of powder g per 100 ml of feed -18.7 g of powder + 90 ml of water

instant oatmeal follow - on dairy nutrition (based on goat 's milk excluding cow proteins) carbohydrates (lactose, dextrose) prebiotic fiber lyophilized probiotic microorganisms dried fruit powders as natural flavorings other components: 43.0 8.0 34.0 6.4 12.2 2.3 2.0 0.4 0.3 0.06 2.8 0.52

vitamins, minerals, trace elements, unsaturated fatty acids in the amounts recommended by the current directive for infant formulas and follow-on formulas protein profile:

total protein content alpha-s1-casein content 12.6 2.4 192.5 mg 36 mg

Example 3

Cereal dairy supplement for children from the end of the 6th month of life in the form of a finished heat-treated (pasteurized) supplement

Component g per 100 ml of finished feed

instant multi - flour flour continued dairy nutrition (based on goat 's milk excluding cow proteins) carbohydrates (lactose, dextrose) prebiotic fiber fruit pulp as a natural flavoring other components: 9.1 8.0 2.1 2.0 7.1

vitamins, minerals, trace elements, unsaturated fatty acids in the amounts recommended by the current Directive for infant formulas and follow-on formulas.

protein profile:

total protein content alpha-s1-casein content 3.0 78.3 mg

Example 4

Milk-based corn food for children from the end of the 8th month of life in the form of instant powder

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Component £ per 100 £ powder £ per 100 ml of feed -35 ε of powder + 90 ml of water instant corn flour 28.7 10.0 follow - on dairy nutrition (based on goat 's milk excluding cow protein) 50.3 17.6 carbohydrates (lactose, dextrose) 7.2 1, prebiotic fiber 2.0 0.4 lyophilized probiotic microorganisms 0.9 0.06 dried fruit powders as natural flavorings 3.0 0.52

other components:

vitamins, minerals, trace elements, unsaturated fatty acids in the amounts recommended by the current Directive for infant formulas and follow-on formulas.

protein profile:

total protein content 18.6 6.5 alpha-s1-casein content 568 mg 199 mg

Industrial use

Processed cereal-based milk or milk-based cereals with an optimized milk component consisting of goat's milk and goat's protein with a modified protein profile ensuring a goat alpha-s1-casein content in the range of 10 to 200 mg of alpha-s1-casein per 100 ml of feedingstuff in the form of instant mixtures renewable for consumption by the addition of water, or as prepared heat-treated foods intended for direct consumption. The use of the food from the end of the 4th month of infant life can be used for targeted physiological induction of the desired oral tolerance of milk proteins, which is an integral part of prevention of allergies and intolerances of milk and dairy products in children and adults.

Claims (2)

PATENT CLAIMS 1. Processed cereal-based milk supplement with a predominant proportion of cereals and an optimized milk component consisting of goat's milk and goat's protein excluding cow's proteins, fortified either singly or in mixtures with vitamins, minerals, trace elements, prebiotics, probiotic microorganisms, nucleotides, polyunsaturated fatty acids , vegetable oils, carbohydrates, natural aromatic and flavoring substances, intended for children from the end of the 4th month of life to increase the oral tolerance of milk proteins, characterized in that said milk component has an alpha-s1-casein content of 10 to 200 mg in 100 ml of administered food to induce oral tolerance of milk proteins in a controlled, natural way depending on the age of the child and the degree of maturation of his digestive tract. 2. Milk-based cereal supplement with a predominant proportion of an optimized milk component consisting of goat's milk and goat protein excluding cow's protein and a proportion of cereals fortified either singly or in mixtures with vitamins, minerals, trace elements, prebiotics, probiotic microorganisms, nucleotides, polyunsaturated fatty acids acids, vegetable oils, carbohydrates, natural aromatic and flavoring substances, intended for children aged from the end of the 4th month of life to increase the oral tolerance of milk proteins, Characterized in that said milk component has an alpha-sl-casein content of 10 to 200 mg in 100 ml of the administered food to induce oral tolerance of milk proteins in a controlled, natural manner depending on the age of the child and the degree of maturation. his digestive tract.