CY1107652T1 - Μεθοδος ποσοτικης ανιχνευσης ζωντων επιθηλιακων κυτταρων ογκου σε ενα βιολογικο υγρο - Google Patents

Μεθοδος ποσοτικης ανιχνευσης ζωντων επιθηλιακων κυτταρων ογκου σε ενα βιολογικο υγρο

Info

Publication number
CY1107652T1
CY1107652T1 CY20061100679T CY061100679T CY1107652T1 CY 1107652 T1 CY1107652 T1 CY 1107652T1 CY 20061100679 T CY20061100679 T CY 20061100679T CY 061100679 T CY061100679 T CY 061100679T CY 1107652 T1 CY1107652 T1 CY 1107652T1
Authority
CY
Cyprus
Prior art keywords
live
tumor cells
quantitative determination
vulcar
body fluid
Prior art date
Application number
CY20061100679T
Other languages
English (en)
Inventor
Ulrich Pachmann
Katharina Pachmann
Original Assignee
Ulrich Pachmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ulrich Pachmann filed Critical Ulrich Pachmann
Publication of CY1107652T1 publication Critical patent/CY1107652T1/el

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Hospice & Palliative Care (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Η εφεύρεση αφορά μια μέθοδο ανίχνευσης επιθηλιακών κυττάρων όγκου σε ένα βιολογικό υγρό με τα ακόλουθα βήματα: α) Ετοιμασία συγκεκριμένης ποσότητας ενός βιολογικού υγρού, β) Σήμανση των ζώντων επιθηλιακών κυττάρων όγκου μέσω προσθήκης στο βιολογικό υγρό αντιανθρώπειων επιθηλιακών αντισωμάτων, τα οποία είναι συνδεδεμένα με μαγνητικά σωματίδια, γ) Σήμανση των ζώντων επιθηλιακών κυττάρων όγκου μέσω προσθήκης στο βιολογικό υγρό αντιανθρώπειων επιθηλιακών αντισωμάτων, τα οποία είναι συνδεδεμένα με ένα φθορισμόχρωμα, δ) Μαγνητικό εμπλουτισμό των ζώντων επιθηλιακών κυττάρων όγκου, ε) Ακινητοποίηση του κατ'αυτόν τον τρόπο προκύπτοντος εναιωρήματος σε έναν φορέα μέσω αυτοτελούς προσκόλλησης των ζώντων κυττάρων όγκου, στ) Καταγραφή των ζώντων επιθηλιακών κυττάρων όγκου μέσω κυτταρομετρίας με σάρωση laser και υπολογισμό του αριθμού αυτών των κυττάρων σε σχέση με την ποσότητα του διαθέσιμου βιολογικού υγρού.
CY20061100679T 2001-06-02 2006-05-26 Μεθοδος ποσοτικης ανιχνευσης ζωντων επιθηλιακων κυτταρων ογκου σε ενα βιολογικο υγρο CY1107652T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10127079A DE10127079A1 (de) 2001-06-02 2001-06-02 Verfahren zum quantitativen Nachweis vitaler epithelialer Tumorzellen in einer Körperflüssigkeit
EP02011919A EP1262776B1 (de) 2001-06-02 2002-05-29 Verfahren zum quantitativen Nachweis vitaler ephithelialer Tumorzellen in einer Körperflüssigkeit

Publications (1)

Publication Number Publication Date
CY1107652T1 true CY1107652T1 (el) 2013-04-18

Family

ID=7687133

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20061100679T CY1107652T1 (el) 2001-06-02 2006-05-26 Μεθοδος ποσοτικης ανιχνευσης ζωντων επιθηλιακων κυτταρων ογκου σε ενα βιολογικο υγρο

Country Status (8)

Country Link
US (1) US20030017514A1 (el)
EP (1) EP1262776B1 (el)
AT (1) ATE319086T1 (el)
CY (1) CY1107652T1 (el)
DE (2) DE10127079A1 (el)
DK (1) DK1262776T3 (el)
ES (1) ES2260353T3 (el)
PT (1) PT1262776E (el)

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US20040058401A1 (en) * 1999-04-13 2004-03-25 Blaise Bossy Method for detecting rare event
US6692952B1 (en) * 1999-11-10 2004-02-17 Massachusetts Institute Of Technology Cell analysis and sorting apparatus for manipulation of cells
JP2006501449A (ja) 2002-09-27 2006-01-12 ザ ジェネラル ホスピタル コーポレーション 細胞分離のためのマイクロ流体デバイスおよびその使用
WO2004077021A2 (en) * 2003-02-27 2004-09-10 Lesko Stephen A Standardized evaluation of therapeutic efficacy based on cellular biomarkers
US20070160503A1 (en) * 2003-06-13 2007-07-12 Palaniappan Sethu Microfluidic systems for size based removal of red blood cells and platelets from blood
EP1776449A4 (en) * 2004-03-03 2009-08-12 Gen Hospital Corp MAGNETIC DEVICE FOR ISOLATING CELLS AND BIOMOLECULES IN A MICROFLUIDIC ENVIRONMENT
WO2006089341A1 (en) * 2005-02-25 2006-08-31 Mcleay Charmaine A C. a. m. detector
US20070026413A1 (en) * 2005-07-29 2007-02-01 Mehmet Toner Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20070026417A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026414A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026415A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20090181421A1 (en) * 2005-07-29 2009-07-16 Ravi Kapur Diagnosis of fetal abnormalities using nucleated red blood cells
US20070059680A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for cell enrichment
US20070026416A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070059781A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
US20070059718A1 (en) * 2005-09-15 2007-03-15 Mehmet Toner Systems and methods for enrichment of analytes
US20070059719A1 (en) * 2005-09-15 2007-03-15 Michael Grisham Business methods for prenatal Diagnosis
US20070059774A1 (en) * 2005-09-15 2007-03-15 Michael Grisham Kits for Prenatal Testing
US7615358B2 (en) 2006-04-20 2009-11-10 Ulrich Pachmann Method for determining the concentration of vital epithelial tumor cells in a body fluid
EP2029779A4 (en) * 2006-06-14 2010-01-20 Living Microsystems Inc HIGHLY PARALLEL SNP GENOTYPING UTILIZATION FOR FETAL DIAGNOSIS
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
CA3069081C (en) 2008-09-20 2023-05-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
AT508806B1 (de) * 2009-10-07 2013-06-15 Onkotec Gmbh Analysegerät und verfahren
US8187979B2 (en) * 2009-12-23 2012-05-29 Varian Semiconductor Equipment Associates, Inc. Workpiece patterning with plasma sheath modulation
WO2013003898A1 (en) * 2011-07-07 2013-01-10 Clinical Genomics Pty. Ltd Method for detection of cancer in a patient
CN102636638A (zh) * 2012-04-19 2012-08-15 上海蓝怡科技有限公司 基于大粒径磁珠的抗原抗体包被方法
US9869013B2 (en) * 2014-04-25 2018-01-16 Applied Materials, Inc. Ion assisted deposition top coat of rare-earth oxide
CN105277697A (zh) * 2015-10-27 2016-01-27 上海芯超生物科技有限公司 一种测定血液中循环上皮肿瘤细胞数量的方法
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DE69417899T2 (de) * 1994-11-17 1999-11-04 Chemunex, Maisons-Alfort Vorrichtung und Verfahren zum Erkennen und Zählen von selten vorkommenden Säugerzellen
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WO1999041613A1 (en) * 1998-02-12 1999-08-19 Immunivest Methods and reagents for the rapid and efficient isolation of circulating cancer cells

Also Published As

Publication number Publication date
ES2260353T3 (es) 2006-11-01
EP1262776A3 (de) 2004-01-02
US20030017514A1 (en) 2003-01-23
EP1262776A2 (de) 2002-12-04
DE10127079A1 (de) 2002-12-12
DK1262776T3 (da) 2006-07-03
PT1262776E (pt) 2006-07-31
ATE319086T1 (de) 2006-03-15
DE50205903D1 (de) 2006-04-27
EP1262776B1 (de) 2006-03-01

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