CS271083B1 - Method of formyl-l-peptides winning - Google Patents
Method of formyl-l-peptides winning Download PDFInfo
- Publication number
- CS271083B1 CS271083B1 CS862351A CS235186A CS271083B1 CS 271083 B1 CS271083 B1 CS 271083B1 CS 862351 A CS862351 A CS 862351A CS 235186 A CS235186 A CS 235186A CS 271083 B1 CS271083 B1 CS 271083B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- formyl
- phe
- ala
- general formula
- peptides
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 3
- DEFJQIDDEAULHB-IMJSIDKUSA-N L-alanyl-L-alanine Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(O)=O DEFJQIDDEAULHB-IMJSIDKUSA-N 0.000 claims abstract description 4
- 108010056243 alanylalanine Proteins 0.000 claims abstract description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims abstract 6
- 150000001412 amines Chemical class 0.000 claims abstract 3
- 238000009833 condensation Methods 0.000 claims abstract 3
- 230000005494 condensation Effects 0.000 claims abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 2
- 125000003178 carboxy group Chemical class [H]OC(*)=O 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 1
- -1 carboxyl-substituted amino moiety Chemical group 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 claims 1
- 150000007942 carboxylates Chemical class 0.000 abstract 2
- 102000005741 Metalloproteases Human genes 0.000 abstract 1
- 108010006035 Metalloproteases Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 235000000421 Lepidium meyenii Nutrition 0.000 description 2
- 240000000759 Lepidium meyenii Species 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 235000012902 lepidium meyenii Nutrition 0.000 description 2
- KHOITXIGCFIULA-UHFFFAOYSA-N Alophen Chemical compound C1=CC(OC(=O)C)=CC=C1C(C=1N=CC=CC=1)C1=CC=C(OC(C)=O)C=C1 KHOITXIGCFIULA-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
i 271 083 OMfCAIlHE H30GPETEIIHÍI K ADTOPCKOMy CBHflETEJIIiCTBy 3aHBKa= No. 3855631 3aíiBjieno: 15.02.85 MKH4s CI2P 21/02
Abtopu: n.M.CTerienoB. JI. A. JIweJiHiiCKaíi h C. E. GoRiiobíi
3a«BHTeJii.: Bcccoio3iiuh nay*ino-HccjieaciBaTejibcKHH HHCTH-ryT reiieTHKH h ceJieKUHH npoMMuiJieiiiibixMHKpOOpralIHSMOB
HajBaHHe H3O<-.[’HTI?HHSI: CnOCOG nanyiEIIHSI Ν-ΦΠΡΜΗΛ-Ε-ΠΕΠΤΗΛΟΒ Μ3οβρβτκΗΗβ otiiochtcsi κ ycoBepuieHCTBoBaimoMy cnocoey nojiy*ieiiH$i Ν-φορμηλ-L -ποπτηλοβoeuieft «opMyjiu
Eor - Λ-Β-Χ ΓΛβ Eor - ΦΟρΜΗΛΙ A - Asp. Met. Ala-Ala. Phe; D - Phe. Leu. X - MeTHJi. - IIN - NO2 . KOTopue MoryT iiaňTH npHMeiieiiHe b 3KcnepHMeiiTajn.noň shoxhmhh η μθληκηκο.
Uej|ž> ιοοβρΕΤβιΐΗϋ - noBuuieHHe euxoaa h pacuiHpeiiHe accopTHMeiiTa Ν-φορμηλ-L - πβτΗΛΟΒ.roMoreHHOCTt kohciiiux coeziHiieiiHfí npoDepnioT c noMouibn tohkocjioKiioH xpoMaTaorpa«HH 11a n-nacTHHKax "CHJiyeoji" <'ICCP) a cncTeMax: 1 - 11 - eyTaiioji - πηρηληη - Boaa - yxcycHan KHcjioTa. 15 = 10:12:3; 2-neTaHOji - xjiopo«opM. 3 = 7. yrjiu BpauteHHX onpeaejixuT Ha cneKTponoasipHMeTpe “llllger'' (AurjiHX).
AmhhokhcjiotiiuR aiiajiHa BunoJiiisiioT na aMHtioKHCJioTiioM aHaJiH3aTope Diirrum D-500 (CIUA) nocjierHApojiHsa nenTHBa b TeneiiHe 24 "lacoa o 3anasuinoíí axnyjfe c 5.7 11. IICI πρη 110°C. B cHHTeae HcnojiboyioT MeTaJiJiciiipOTeHiiaay Bac. thermoproteolytlcus φηρμη “Ierva" (ΦΡΓ) -
TepMOJIH3Hll. H3O<speneiiHe HjuuocTPHpyeTCa cjieaynuiHMH HPHMepaMH.npHMep 1. Π -ΗΗΤΡΟβΗΗΛΗΛ - N -♦OPMHJI-L-MeTHOHHJI-L-J«eňUHHa<l ).
Cxecb 0.354 r. (2 mmojib) Ν-φορμηλ- L -ΜβτΗΟΗΗΗβ H 0,502 r (2 MMOJib) π -HHTpoaiiHJiHaa L-JieňuHiia pacTBOpsusT b 1.5 mji AHMeTHjieopMaMHAa (ΑΜΦΑ). npx nepeMeuiHBaHHH MeajieHHO npHeaejisnoTp5,0 mji 5.10-2M pacTBopa xJiopHCToro KajibUHSi. λοβθλθιιηογο λο pH 7.1 0.1 li. NaOH, h 3aTeM πρηnepeHeuiHBaiiHH aoaaBJínnT 5 μγ (l.SS.lO-^MHoab) TepMojiHOHiia. PeaKUHOHHyu cMecb nepeMeuiHoaioT 6 <iπρη 20°C h ocTaeJisiiOT na no*ib B xojiOAHJibHHKe. OunaBiUHň ecaaox οτΦΗ-nbTpOBwnaioT προμμβλιοτ 0.5H.llCl (2 x 0.5 mji). eoaoň <2 x 0.5 mji). 0.5 H.NalIC03<2 x 0.5 mji). βολοΑ <2 x 0.5 mji) h cyuiaTb BaxyyMe. nojiy'ia»T 0,784 r (95.5¾) coeaHHeiiHsi 1 c TOMiiepaTypoií nJiaBJíeiiHsi 164°C. Rí0.77 (1), 0.54 (2); í eó i£e “+5.5° <c 0.5; ΛΜΦΛ). AmhhokhcjiotiiuH aitaJiHa: Met 0.89 (1): Leu 1,1’. (1).
IlpHMepu 2-4 ocytuecTBJísiioT aiiajiorHHHo npHMepy 1 c τβΜ οτ-ιτημμηθμ hto HCxoaHasi meči. coaepwHT 0.63 MMOJib Ν-φορμηλ- L -MeTHOHHHa h 0.63 mmojib n—HHTpoaHHJiHaa L-JieňuHna b 0.6 mjiΑΜΦΑ h 2.0 mji 0.1 M UHTpaTiioro eyeepa. a τθρμιμιη3ηη eepyT b KOJiH»iecTBe 2.5 μγ<0.63.10~'4MMOJib) h cxecb BuaepiMHBaiDT 2 xaca πρη 20°C. ΠρΗΜβρω 5-9 ocyiuecTBJíHMT anajiortriHO npHMepy 1 c τθμ οτληηηθμ. hto Hcxoanan peaKUHomiasi CMecb coaepxiHT 0,63 mmojib Ν-φορμηλ- L-MeTHoiiHiia h 0,63 mmojib n-iiHTpoatiHJiHaa L-jieHunna b 2.0 mji 0.05M eopaTiioro ey-tepa, a TepMOjiH3Hii eepyT a KOJiHMecTee 2.5 μγ (O.ĎB.lO—^MMOJib) h peaKUHOHHyn cMecb BuaepwHBaioT 2 ’iaca npw 20°C. 271 003 2 B npHMepax 2-9 H3y>iawT 3aBHcxMocTi> buxoab uejieeoro npoayKTa ot pH peaKUHamioň cpew.nojiy'ieiiiiue peayjibTaTU npHBeAexu b TaejiHiie 1.
TafiJiHua 1 (lpXMep pil CPCAM Buxoa.% 1 2 3 2 5.6 1.97 3 6.0 8.8 4 6.5 17.2 5 7.0 77.0 6 7.3 79.8 7 7.5 84.4 8 8.0 70.3 9 8.4 60.4 ΠρΗΜβρ 10. n-IlHTpoaiiHJiHa - N - «opmhji - L - ajiaiiHJi - L - ajiaiiHA - L - «eXHJiaAaiiHHa (11).
Cmoci, 0.180 r <1 mmojib) N -ΦορΜΗΛ- L-ajiaHHJi- L-ajiaHHJia x 0.290 r <1 μμολ)n-HHTpoaiiHJiHaa N -«eiiHJiaJiaiiuna pacTBOpsnoT o 0.4 mji ΛΜΦΑ, npx nepeMeiuHBaxHx MeAJieiixonpHeaBJínioT 0.6 mji 1.10“3M pacTeopa CaCIa. aoBeAexxoro no pH 6.9 0,1 H.NaOH, x oaTeM npxnepeMeuiHoaiiHH AoaaBJíflUT 40 mkji 1 mM pacTBOpa TepMOJiH3Hiia (4.10~5mmojii<) b 1.10-3M pacTBopcCaCl2 c p|{ 6,9. PeaxuHOHxy» cMeci. BUAepwxBaioT npx nepeMeuiHBaHxx 24 Maca. ot»hai>tpobubu»t
BunaBUixR ocaaoK. npOMMBanT ero. ksk onxcano b npxMepe. x cyuiaT b BaxyyMe. nojiyxaiOT 0,35 r <85£) cocAHiieiixn 11 c TeMnepaTypoH nJiaBJíeHXX 201°C. Rt 0.94 (1). 0.88(2); C Z/ ]^θ - -7.5° <c 0.5 ΑΜΦΑ). ňMHHOKxcJioTHuň axajixs: Ala 1.99 (2): Phe 1.01 (1). npxMepu 11-12 eicyuiecTBJííiioT aiiaJiorxMiio npxMepy 10 c τβΜ OTJiHMHeM, ‘ΐτο τβΡΜθΛΧ3Η„XCnOJU.3y»T B KOJIXMeCTBe 1.10”B X 2.10-B MMOJII, ΟΟΟΤΒβΤΟΤΒβΗΧΟ.
DpxMepu 13-14 ocyuiecTBJííiioT anajiorx'ixo npxMepy 10 c tbm οταημηθμ, mto peaKUHOHHax cMectcoAepwxT 0,6 mji 1.10-3M pacTBOpa CaCl2 x TepMOJixaxx eepyT b KojixMecTBe 6.10-s x 10.10-5 mmojii,cooTBeTCTBemio. B -npHMepax 11-14 H3y»ia»T aaBHCHMOCTi, βμχοαο uejieeoro npoayxTa ot KOJixnecTBa TepMOjiX3Hiia.nojiyMeuxue peoyjtBTaTu npHBeAexu b TaejiHue 2.
Taejixua 2 ΠρΗΜβρ KOJIHMOCTBO TepMOJTHBHIia . MMOJIB Buxoa.% 1 2 3 11 1.10~B 40 12 2.10-® 64 13 6.10-S 85 14 1.ío-4 95 ΠρΗΜβρυ 15-19 ocyuiecTBJíxuT anajiorx*ino npHMepy 10 c τβΜ οτλημηθμ. mto peaKUxoHiiyn CMeci,BMJtepwxBauT npx pa3JiMMHMX TeMnepaTypax b xHTepeaJie ot 6 ao 45°C. B πρχΜθτβχ 15-19 xayiaioT3aBHCHMOCTi> BuxoAa nejiesoro npoAyKTa ot TeMnepaTypu peaKUHOHHoR cMecx. nojiyMeHHNe peayjibTaaTunpHBeAexu b Taejixne 3. 271 083
TaejiHua 3 ΠρκΜθρ TeMnepaTypa peaKUHomioH CMecH.°C Biixoa. % 1 2 3 15 6 62 16 15 85 17 22 93 18 37 80 19 45 51 npHMepu 20-23 ocyutecTBJímoT atiajiorHMHo npHMepy 16. B npHMepax 20-23 H3yMa»T aaeHCHKOCTi. Buxofla uejieBoro npoayKTa οτ BpeMeHH peaKUHH. nojiy»ieHHue pe3yjibTaTM npHBeaenu b TaejiHiie 4. Taejiuua 4 ΠρΗΜβρ ΒρβΜΠ peaKUHH. m Buxoa.% 1 2 3 20 3 54 21 6 64 22 48 85 23 72 90 npHMepu 24-26 ocytuecTBJísnoT aiiajioriemo npHMepy 10 c Ten οηίΗΜΗβΜ, mto cHeci. hcxoaiimx ΚΟΜΠΟΗβΗΤΟΒ paCTBOpSIlOT B 0.2 MJI UM® Λ H 0,3KOJiwiecTBe 20 mkji 1 mM pacTBOpa <2.1Q-B>.ueJieBoro npoayKTa ot MOJisipuoro coaTiiouieiiHiinajiyHemiue peoyjibTaTU npHBeaeiiu b TaeJiHiie 5. mji bojím npM pil 6,7 TepMOJiH3HH aoeatuiíiioT nB npHMepax 24-26 H3y»iaioT aaBHCHHOCTi, euxoaaaMHHOKOHnoiieiiTa h KopeoKCHJibiioro KOMnoiieiiTa.
Taajiuua 5 KOJIHHeCTBO , MMOJIb ΠρΗΜβρ - BUXOfl.*
KapiSOKCHJIb- aMHHOKOH- ΗΟΓΟ ΚΟΜΠΟ- ΠΟΗβΗΤβ ΗβΗΤβ 1 2 3 4 24 • 0.8 1.0 96 25 1.0 2.0 22 26 1.0 0.8 70 ΠρΗΜβρ 27. n-IlHTpoaiiHJiHa Ν-φορμηλ L-aJiamui- L -aJiaHHJi- L -JieftUHHa <III>. CMecb 0,188 r <1 mmojib) Ν-φορμηλ L-ajiaHHJi- L -ajiailHHa H 0,21111 r <1 MMOJib) n-iiHTpoaiiHJixaa L-jieňuHHa pacTBopfWT b 1 mji ΑΜΦΑ. Μβ/υιβΗΗΟ πρη πβρβΜβηΐΗΒβΗΗΗ aoeaBJíxioT 5 mji 5.10-2M pacTBopa
CaCl2. πρη nepeMeuiHBaHHH aoeaBJíniDT 5 μγ <1.33 x 10-4 mmojib) TepMOJiH3HHa. PeaKUHOHnye CMeci
BuaepMtHBaioT 6 Macoe πρη 22° C h noMeutanT Ha komb b xojioaHJibiiHK. BunaBuiHň ocaaoK οτΦΗΛί,τροΒΜΒβιοτ, npoMUBauT. xax onHcano b npHMepe 1. cyuiaT b BaKyyMe. 271 083 4 ílojiyMaioT 0.305 r (72,5¾) coejiHiienHH III c TeMnepaTypofi njiaBJíeiiHíi 176°C. Rf 0.71(2): t e>(y 1^® - -21° (c 0.5: ΑΜΦΑ). AmhiiokhcjiothuB anajiM3= Ala 1.93 (2), Leu 1.08 (1). Πρημθρ 28. n-IlHTpoaiiHJtHa N-*opmhji- L - ®eiiHJiajiaHHji-jiefiUHiia (IV). CMecb 19 ΜΓ (0.1 MMOJib) Ν-φορμηλ-L - «eiiHJiajiaHHiia M 25 μγ (0.1 mmojib) n-HHTpoaiiajinaaL-jteBuHiia pacTBopsuoT b 0.15 mji ΚΜΦΛ, Meajieiiiio πρη nepeMeiunaaiiHH npH<saBJi$uoT 0.2 mji βολμ (pil6.8). 3βΤβΜ npHOaBJIXlOT 0,7 ΜΓ (2 X 10" SMMOJlb) TePMOJIH3HHa H BUaepWHBalOT ΠΡΗ KOMllaTHOBTeMnepaType 24 Maca. OunasuiuB ocaaoK pacTBopsuoT b 5 mji sTHJianeTaTa m npoMUBanT 0.5 h jihmcihiiohkhcjiotu (1 x 1 mji), 0,5 H.NaIIC03 (1 x 1 mji). cyuiaT nají cyjib*aTOM ιιβτρκίΐ h ynapHBanT bBaxyyMe. nojiyMa»T 27 μγ (66¾) οοθληηθιιηπ iy c TennepaTypoB njiatuienHíi 181°C, Rt 0.81 (1). 0.91(2): [ eó· - -16° (c 0.5: ΑΜΦΑ). AmhhokhcjiotiimB aiiajiH3'· Leu 1,0 (1): Phe 1.0 (1). Πρημθρ 29.
MeTHJiOBuB 3ΦΗΡ Μ -φορμη-τι- L -acnapTHJi- L - *eiiHJiaJiaHHiia (Y). K 2.1 r (10 mmojib) xjioprnapaTa μθτη.ποβογο 3®npa N- ®eHHJiajiaHmia npHeaBJínioT 0.66 r (4.1MMOJib) N -φορμηλ- L -acnaparHHOBoň khcjiotu b 2.0 mji bojím. Ilocjie pacTBOpeHHsi hcxojihuxκοΜΠΟΗβιιτοβ pH peaKUHOHHoB CMecH ΛΟΒΟΛίΐτ ao 6.6-6.8 npHaaBJíeiiHeM 6 h. NaOH. 3btgmoepaeaTUBaMT 20 μγ (5,3 x 10-4 mmojib) TepMOJiH3Hiia h nepeMewHBanT 24 Maca πρη 25°C. OcajioxpacTBOpaiOT o 25 mji IM yxcyciioB khcjiotu h oepaeaTUBaMT 30 mji SP-ce®aaexca. CMOJiyOT®HjibTpoBUBaioT, npoMUBaNT 1 M yxcyciioB khcjiotu. Πθπτηα 3KCTparnpyi0T cMecbn oTHJiaueTaT - bojib(9=1) (8x8 mji). OebeaHHeHHuB bkctpskt ynapneauT b BaxyyMe. Macjio KpHCTajuiHoyeTcsi na xowoaeΠΟΛ 3ΦΗΡΟΜ. noJiyMaioT 0.42 r (35¾) coeAHiieiiHsi V: £ oč* y® · -24.5° (c 0,5: H2O): £ l18 - -15° (c 1,0: AcOH). AmhiiokhcjiothuB aiiajiH3: Aspl.03 (I), Phe 0.97 (1). Πρημθρ 30.
MeTHJiOBuB 3ΦΗΡ N--»opmhji- L - acnapTHJi- L -®eiiHJiaJiaiiHiia (Y). ÍIojiyuaioT anajiorHMHo npHMepy 29, hcxoah H3 2 mmojib xjioprHpaTa μθτηλοβογο οφηρβ L-®eiiHjiaJiaHHiia η I mojib Ν-φορμηλ- L -acnaparHiioeoH khcjiotu. Ajih BuaeJieHHH uejieeoro npoayxTa xpeaKUHOHiioB cmbch npHeaBJínnT 4.5 mji yxcyciioB khcjiotu. a 3aTew 40 mji oTHJiaueTaTa. BunaewHHocaaoK οτΦΜϋΒτροΒΗΒβητ. opraiiHMecxyio ®aay προμμββμτ bojioB (7 x 2 mji). cyuiaT iiaa σν4ΐΒΦΒΤθΜιιβτρΗΗ h ynapHBanT b BUKyyMe. nojiyianT 0.14r (43¾) coeaHHeHHsi Y.
KaK bhjuio H3 npHBeaeiuiux πρημθροβ. onucuBaeMuB cnocoe noasojisieT nojiyuaTb οπτημθοκηMHCTUe N -®OPMHJ|- L -nenTHJlU B ΜΪΙΓΚΗΧ yejlOBHHX 603 SaUlHTU 6OKOBUX eyilKUHOHaJlblIUX rpyrinHPOBOK. 5 271 0B3
•ΟΡΜΥΛΛ H30BPETEIIHB
Cnocoe nojiyneHHa Ν-φορμηλ - L -πθητηλοβ oemeň »opHyjiu
For -Λ-Β-Χ.
ΓΑβ For - 'KIPMHJG n - Asp, Met.Ala-Ala. Phe: B - Phe,Leu.
BKJi»'ia»uiHň KOHíejicaiiHio N-*opMHJi3amniueiiHoro KapeoxciuibHoro κοΜΠοκβΗΤβ c aMHHOKOMnoHetrroM.BaiuHiueHHMM KapeoKCHJibHoR rpynne. OTjiH'ia»iuHflcq τβΜ. ίτο, c uejibn noBumenHfl βμχοαβ 11pacuiHpeHH$i accopTMMeHTa ueJieeux npoAyKToa, a KanecTBe K -®opMHJi3auiHmeiiHoro KapeoKCHAbtioroκοΜΠΟΗβιιτβ KcnojibayioT coeanneniie oemeň ΦορΜν-πΜ
For -Λ-ΟΗ.
rae For h A ΗΜβητ 3naMeiiHH. yKa3aiiuue Buuie, a a xanecTBe aMHHOKOMnonenTa - coeíHHeiiHe oemeňBOpMyJlU
H-B-X rae B η X ΗΜβητ 3HaneHH$i. ytcaaaHHMe euiue. h KOHAencauHn προβοαατ b npHcyTCTBHH 2.10-5 -1.10-·4MMOJib MeTaAAonpoTeHi<a3u Bac. bhermoprobeolytlcus na 1 mmojib KapeoKCHJibnoro κοΜποιιβιιτβ πρη pH6,5-8,0. TexnepaType 15-37°C a boaiioB hjih ΒΟΑΗΟ-ΑΗΜβΤΗΑΦΟΡΜβΜΗΑΗοΑ cpeae πρη mojisiphomCOOTHOUieHHH aMHlIO- H KapeoKCHJibHoro ΚΟΜΠΟΙΙΘΙΙΤΟΒ 1=1 - 1:2. HCTOHHHKH ΗΗΦΟρΜβΙΙΗΗ. ΠΡΗΗΛΤΜβ BO BHHMalIHe ΠΡΗ 3ΚΟΠβρΤΗ3β= DE. B. 2256055. 271 083
P E · Ε Ρ Λ T CI1OCOB ΠΟΠΥΊΕΙΙΗΗ N-SOPMyJI- L - ΙΙΕΙ1ΤΗΛΠΒ H3o<speTenHe KacaeTcq 3aMeuteiinux πβπτηλοβ, b "ibcthocth Μ-φορμηλ- L - πθπτηλοβ oemeH«opMyjiu 1: R - A - B - X, rae R - φορμοιι. A-Asp. Met. Ala-Ala. Phe; B-Phe.Leu; X - mbthji . -HN- CeíU-n-NOz, κοτορΝβ μογυτ Ha Rtu πρημθιιθιιμθ b 3KcnepHMeiiTajibHoň 6Moxhhhh h MeanitHiie.(loBuuienHe auxoaa h pacuiHpeHHe accopTHHeHTa 1 aocTHraeTcn hobumh hcxoahmmh npoayKTaMH honpeaejieiuiMMH ycjioBHííMH npoBeaeiiHsi peaKUHH. CHiiTea 1 seayT KOHaeHcauHeň N-*opMHJi3amHmennoraKapňOKcmii.iioro KOMnaiieiiTa <KK) orsmeR «opMyjiu: R-A-OII c aMHHQKOMnoiienTOM oeuieři <i>opMyjiU: II-B-X,r«e R.A.B η X yKaaanu Buuie, 3aiuHmeniiuM na KapeoKCHJibiioti rpynne. Ilponecc aeayT b npHCyrcTBHH(2.10"s )-<l. 10~4) mmojib MeTajiJionpOTeHna3U Bac. thermoproteolytlcus na 1 MMOJib KK iiph pH6.5-8.0, TewnepaType 15-37°C b BonHoň hjih βοληο-λημβτηιιφορμβμηληοΗ cpeae πρη mojixpiiomcooTHOUieiiHH aMHHOKOMnoneHTa h KK. paBHOH 1:1-2. Cnocoe oeecneMHBaeT noJiyueHHe οπτηίθοκηMHCTUX 1 B ΜΒΓΚΗΧ yCJlOBHBX ββ3 3aUIHTU 6HKOBUX ®yHKAUHOHajlbHUX Tpynn C BUCOKHM ΒΗΧΟΛΟΜ (ao96*). 1
Claims (1)
- 7 271 083 PŘEDMĚT VYNALEZU Způsob získávání N - formy 1 • - L - peptldů obecného vzorce For - A - B - X . kde For le formyl ; A - Asp. Met, Ala - Ala. Phe : B - Phe. Leu X - methyl. - HH -- NOa zahrnulící kondenzaci H - formy 1substituované karboxylové složky s amlnosložkou substituovanou v karboxylové skupině, v y z n a č u 1 í c í se tím, že za účelem zvýžení výtěžku a rozSíření sortimentu výsledných produktů se lako N - formysubstltuovanákarhoxylová složka využívá sloučenina obecného vzorce For - A - OH . kde For a A malí výge uvedené významy a lako aminová složka le sloučenina obecnéhovzorce Η - B - X . kde B a X malí významy, uvedené výge a kondenzace se provádí v přítomnosti2.10-B až 1.10“é mmol metaloprotelnázy Bac. thermoproteolytlcus na 1 mmol karboxylovésložky při pH 6.5 až 8.0 , při teplotě 15 až 37 °C ve vodním nebo vodně N.N - dlmethyl formámldovém prostředí při molárním poměru amlno - a karboxylových složek1 1 až 1 : 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862351A CS271083B1 (en) | 1986-04-03 | 1986-04-03 | Method of formyl-l-peptides winning |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862351A CS271083B1 (en) | 1986-04-03 | 1986-04-03 | Method of formyl-l-peptides winning |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS235186A1 CS235186A1 (en) | 1989-05-12 |
| CS271083B1 true CS271083B1 (en) | 1990-08-14 |
Family
ID=5360405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS862351A CS271083B1 (en) | 1986-04-03 | 1986-04-03 | Method of formyl-l-peptides winning |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS271083B1 (cs) |
-
1986
- 1986-04-03 CS CS862351A patent/CS271083B1/cs unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS235186A1 (en) | 1989-05-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Thomas | Electron spin resonance study of iminoxy free radicals | |
| CY1107534T1 (el) | Αναμειξιμα με νepο διαλυματα αζαλιδων | |
| Turner et al. | Ovothiol: a novel thiohistidine compound from sea urchin eggs that confers NAD (P) H-O2 oxidoreductase activity on ovoperoxidase. | |
| Badet et al. | Time-dependent inhibition of Bacillus stearothermophilus alanine racemase by (1-aminoethyl) phosphonate isomers by isomerization to noncovalent slowly dissociating enzyme-(1-aminoethyl) phosphonate complexes | |
| ATE168662T1 (de) | Stabilisierte orthokieselsäure enthaltende zubereitung sowie biologische zubereitung | |
| WO2010040880A1 (es) | Procedimiento de síntesis de pentasacáridos desprotegidos a partir de un pentasacárido precursor protegido | |
| CS271083B1 (en) | Method of formyl-l-peptides winning | |
| AU1678600A (en) | Process for extraction of an organic salt from plants, the salt, and other similar salts | |
| Bencic et al. | ATP levels of chinook salmon (Oncorhynchus tshawytscha) sperm following in vitro exposure to various oxygen tensions | |
| Beyer et al. | ‘Benemid', p-(di-n-propylsulfamyl)-benzoic Acid: Inhibition of Glycine Conjugative Reactions | |
| Bai et al. | A simple effective procedure for the determination of adenosine triphosphate in soils | |
| Moore et al. | Peptide hydroxamic acids inhibit skin collagenase | |
| BERG et al. | Presence of trimetaphosphatase in the intestinal mucosa and properties of the enzyme | |
| TR200100272T2 (tr) | Zofenopril kalsiyum tuzunun hazırlanmasına mahsus bir yöntem | |
| ATE250974T1 (de) | Flüssiges verdickungsmittel für oberflächenaktive systeme | |
| Holmes et al. | The mass spectra of benzamide and thiobenzamide | |
| Filippov et al. | Synthesis of a nucleopeptide fragment from poliovirus genome | |
| Kamiryo et al. | Sequential addition of glycine from glycyl-tRNA to the lipid-linked precursors of cell wall peptidoglycan in Staphylococcus aureus | |
| RU95114359A (ru) | Способ и промежуточные продукты для получения простых гидроксиламиновых эфиров и их солей | |
| English et al. | Metalloprotein electron-transfer mechanisms. Quenching of electronically excited tris (2, 2'-bipyridine) ruthenium (II) by reduced blue copper proteins | |
| ATE251630T1 (de) | Phosphinic pseudopeptide als matrix metalloprotease-inhibitoren | |
| ATE178603T1 (de) | Substituierte heteroarylalkylthiopyridine zur bekämpfung von helicobacter bakterien | |
| Hawtrey et al. | Release of Peptide Chains from Rat-liver Polysomes by the N, N-Dimethyl-p-methoxy-L-phenylalanyl Analogue of Puromycin | |
| AU3424899A (en) | Method for laminaribiose synthesis | |
| GR74471B (cs) |