CS254252B1 - Method of amino acids' content increase in grains of cereals and pulses - Google Patents

Abstract

A method of increasing amino acid content in cereal grains and legumes carried out by sprinkling the germinating grain with an aqueous solution urea or ammonium nitrate. Essence The solution is to have the grain in the initial germination phase is applied 24 to 168 hours an aqueous solution of urea or nitrate ammonium during the 6 days of the individual nitrogen-based doses are as follows: Day 1 0.01 to 0.04 wt%, Day 2 0.02 up to 0.07 wt%, day 3 0.03 to 0.1% on day 4, 0.03 to 0.12 wt%, day 5 0.04 to 0.23 wt.%, Day 6 0.04 to 0.24 wt.% While ventilating at ambient temperature, the aqueous solution containing mineral supplement.

Description

The present invention relates to a method for increasing the amino acid content of a grain by sprinkling a grain of cereals and legumes in the initial phase of germination with an aqueous solution of urea and ammonium phosphate.

Nowadays it is commonly used to increase the nutritional value of feed materials such as toast, burn-out, fermentation or grain germination. The germination of cereals is known from the time when the quality of feed was lacking in agricultural practice because of the fact that the nutritional value of cereals significantly increases in germinating grains. The changes that occur during grain germination, ie activation of the grain enzymatic apparatus, cleavage of polysaccharides into smaller subunits, and cleavage of protein chains into more easily digestible segments are currently used in the production of barley malt.

In the feed industry, soybean meal is currently used as a protein component of plant origin in compound feed. However, the economic disadvantage of soy meal compels agricultural practice to search for other proteinaceous components.

The aforementioned drawbacks are eliminated by the method of increasing the amino acid content of cereal and legume grains, which consists in the gradual application of the urea or ammonium phosphate aqueous solution to the grains in the initial germination phase over a period of 24 to 168 hours in an amount calculated as nitrogen. 01 wt. up to 0.04% by weight, on the second day 0.02 to 0.065% by weight, on the third day 0.03 to 0.1% by weight, on the fourth day 0.03 to 0.12% by weight, on the fifth day 0.04 up to 0.23% by weight, on the sixth day 0.04 to 0.24% by weight, based on the dry matter of the germinating grain, at ambient temperature with simultaneous ventilation.

The aqueous solution contains a mineral additive, wherein the individual components are present in an amount of 0.001 to 0.1% by weight. % potassium iodide, 0.001 to 0.1 wt. % ferric sulfate, 0.001 to 0.1 wt. % copper sulfate, 0.05 to 0.3 wt. % zinc sulfate, 0.05 to 0.4 wt. % manganese sulfate, 0.001 to 0.01 wt. % cobalt sulphate and 0.001 to 0.1 wt. molybdenum nu ammonium. ' the advantages of this method of increasing the amino acid content are demonstrated by the fact that the inorganic nitrogen and urea nitrogen are biotransformed to the amino acid nitrogen by utilizing the grain enzymatic apparatus itself. The amino acid content of the grain treated according to the invention increases, depending on the grain type, in the range of 3 to 6% by weight. Germination also increases the content of vitamins, eg the content of vitamin C in peas increases by about 1000% by weight. after 5 days of germination. The content of riboflavine is increased 2 to 3 times, the vitamin content ^is increased after 4 days of germination on 500% by weight .. The content of vitamin K also increases significantly, as evidenced by the literature data. The enzymatic apparatus is activated, which increases the nutritional value of the feed when fed to poultry. Natural biopolymers - such as proteins and polysaccharides - break down into more digestible segments.

These germinated grains are intended in the feed industry as protein components of compound feed, whether dried or not.

Undried germinated grains can also be used for shaping granular feeds, where they act as a binder while maintaining their high nutritional value. It can be said that the grains prepared according to the invention can fully replace the protein components of the feed mixtures that are imported from the capitalist states. Grain can be germinated in all types of malting equipment, whether it is a classical method on the barrels or mechanical germs with automatic process control. In addition, the only step is to sprinkle aqueous solutions of urea and ammonium salts at regular intervals with subsequent overlaying or overlaying, as this will result in an even distribution of the solution. Animals receive the treated grains in any form without any problems and it can be assumed that increasing the vitamin content of the germinated grains will contribute to the improvement of the health condition.

Example 1

0.74 L of water was gradually applied per kg of F2 maize. As soon as the first signs of germination appeared, ie the bright spot at the beginning of the germ, an aqueous solution containing urea was applied at regular time intervals to the germinated grain at: 0.03% on day 1, 0.06% on day 2 on day 3, 0.1% by weight, on day 4 0.13% by weight, on day 5 0.14% by weight, and on day 6 0.14% by weight; Urea per kg dry weight of germinating grain. A total of 0.6 wt.% Was applied to the grain in a six-day cycle. urea per kg dry matter grain. The wetting solution was enriched with a mineral composition of 0.001% by weight. % potassium iodide, 0.08 wt. % ferrous sulfate, 0.03 wt. % copper sulfate, 0.12 wt. % zinc sulfate, 0.25 wt. % manganese sulfate, 0.002 wt. % cobalt sulphate and 0.001 wt. ammonium molybdate.

Amino acid content of maize F2-Pioneer (in% by weight of dry matter of sample)

Aminoacid Original corn Corn. key. with water Corn. key. with urine. aspartic acid 0.73 0.77 1.09 threonine 0.34 0.35 0.42 serine 0.46 0.46 0.51 glutamic acid 1.98 2.12 2.54 proline 0.63 0.65 0.73 cystine 0.12 0.14 0.14 glycine 0.33 0.35 0.44 alpha-alanine 0.75 0.83 0.99 valine 0.45 0.50 0.56 methionine 0.16 0.19 0.18 isoleucine 0.29 0.35 0.40. leucine 1,25 1.29 1.31 tyrosine 0.36 0.39 0.47 phenylalanine 0.43 0.55 0.78 histidine 0.36 0.36 0.37 lysine 0.30 0.31 0.37 ammonia 0.10 0.12 0.21 arginine 0.42 0.42 0.45

total amino acids 9.46 10.15 11.96 Example 2 Per kg of Slavia wheat was gradually 0.7 l of water was applied. After the discovery of the first stamps germination was germinating grain in regular sprinkled with aqueous solution over time intervals urea. The total dose over 5 days was 0.64% by weight. urea per kg of germinated dry matter grains taking a dose was distributed as follows: on the first day 0.04 wt Day 2, 0.08% by weight, On day 3, 0.14% by weight, Day 4 0.18 wt. Day 5 0.2% wt. Amino acid content of Slavia wheat (in% of dry weight of sample) Aminoacid Original wheat Wheat key. Wheat key. with water with urine. aspartic acid 0.61 0.72 1.59 threonine 0.34 0.35 0.39 serine 0.62 0.63 0.62 glutamic acid 4.26 5.17 5, ^r ' proline 1.10 1,22 1,. cystine st. st. <-. glycine 0.50 0.52 0.58 alpha-alanine 0.42 0.45 0.60 valine 0.55 0.55 0.57 methionine 0.15 0.16 0.17

Continuation table

Aminoacid Wheat original Wheat key. with water Wheat key. with urine. isoleucine 0.39 0.43 0.46 leucine 0-, 92 0.92 0.95 tyrosine 0.32 0.40 0.45 phenylalanine 0.53 0.55 0.56 histidine 0.36 0.33 0.35 lysine 0.38 0.31 0.39 ammonia 0.36 0.32 0.34 arginine 0.69 0.62 0.74 total amino acids 12.50 13.65 15.54 Example 3 Per kg of peas was 1 l of water was gradually applied. After discovery the first signs of germination, this is the bright spot at the beginning of the germ, it was to the germinated grain at regular time intervals amount of urea-containing aqueous solution in total dose for 6 days 1.59 wt. Urea per kg dry matter germinating grain. This dose was distributed as follows: on day 1, 0.08% by weight, Day 2 0.13% by weight, 3 . day 0, , 2% by weight, Day 4 0.24 wt.%, 5. day 0.46% by weight, day 6 0.48% wt. Amino acid content in Tolar peas (in% of dry weight of sample) Aminoacid Original peas Pea key. Pea key. with water with urine. aspartic acid 3.1 3.7 4.0 threonine 1.0 0.9 1.0 serine 1,2 1,2 1.3 glutamic acid 4.7 5.2 7.2 proline 0.8 0.8 0.9 glycine 1.1 1.0 1.1 alpha-alanine 1.1 1,2 1.4 valine 1.1 1.3 1.4 methionine 0.2 0.2 0.3 isoleucine 1.0 1.0 1.1 leucine 1.9 1.9 2.1 tyrosine 1,2 1,2 1.4 phenylalanine 1.3 1.4 1.6 histidine 0.8 1.1 1.1 lysine 1.9 1,8 2.1 ammonia 0.3 0.4 0.6 arginine 2.8 2.5 2.9 total amino acids 25.5 26.8 31.5

Example 4

A total of 1 liter of water was applied per kg of Inovec bean. After the first signs of germination, the grain was sprinkled with an aqueous solution of ammonium phosphate at a total dose of 4.44% by weight. each day during six days, 0.74 wt. aqueous ammonium phosphate solution.

Amino acid content of Inovec bean (in% by weight, sample dry matter)

Aminoacid Bob original Bob key. with water Bob key with (NH ₄ ) ₂ 3 aspartic acid 3.34 3.72 4.18 threonine 1.09 1.11 1.13 serine 1.52 1.58 1.71 glutamic acid 4.84 5.02 5.41 proline 1,21 1.34 1.24 cystine 0.24 0.24 0.24 glycine 1,23 1.26 1,28 alpha-alanine 1.08 1.30 1.30 valine 1.30 1.36 1.37 methionine 0.38 0.35 0.30 isoleucine 1,23 1.20 1.17 leucine 2.39 2.36 2.35 tyrosine 1.08 1.10 1.24 phenylalanine 1.32 1.42 1.33 histidine 0.87 0.93 0.94 lysine 2.03 2.06 2.05 ammonia 0.55 0.52 0.59 arginine 2.82 2.90 3.16

Thus, the total amount of amino acids for Inovec bean was 28.52% by weight, 29.77% by weight for bean germinated, and 30.99% by weight for ammonium phosphate bean.

Example 5

A comparative fattening test was performed on two groups of broiler chickens (ROSS) fed a soya-corn diet. The control group was fed a basic diet supplemented with 10 wt. of the original untreated pea, whereas the experimental group received a similar diet with the supplement of 10 wt. sprouted peas (in an aqueous urea solution for 5 days). The experiment showed that chickens in the experimental group had statistically significantly higher body weight gains in the index of 15.5% by weight. compared to the chickens of the control group, while reducing feed consumption per unit of index increments of 9.2% by weight.

Claims (2)

SUBJECT OF THE INVENTION A method for increasing the amino acid content of cereal and legume grains, characterized in that an aqueous solution of urea or ammonium phosphate, calculated on nitrogen in amounts of 0.01 to 0.04% on day 1, is gradually applied to the grains in the initial germination phase for 24 to 168 hours. wt., Day 2 0.02 to 0.07 wt%, Day 3 0.03 to 0.1 wt%, Day 4 0.03 to 0.12 wt%, Day 5 0.04 to 0 23% by weight, on day 6 0.04 to 0.24% by weight, based on the dry weight of the germinating grain at ambient temperature, with simultaneous ventilation, the aqueous solution further comprising a mineral supplement containing 0.001 to 0.1% by weight. % potassium iodide, 0.001 to 0.1 wt. % of ferrous sulfate, 0.001 to 0.1 wt. copper sulfate, 0/05 to 0.3 wt. % zinc sulfate, 0.05 to 0.4 wt. % manganese sulfate, 0.001 to 0.01 wt. % cobalt sulfate, 0.001 to 0.1 wt. ammonium molybdate.