CS218857B1 - Process for saccharification of starch raw material in the production of alcohol - Google Patents

Abstract

Method of saccharification of starch raw material in the production of alcohol. To intensify the saccharification processes, and also to reduce the consumption of substances necessary for saccharification, which is carried out using a mixture of the strain ENDOMYCOPSIS fibuligera Dekker-21, a producer of glucoamylase and the bacterial culture Bac. mesentericus PB, a producer of amylase, in a ratio of 6 to 8 units / ml Gla and 1.0 to 1.5 units / ml AS per lg starch. From the species Bacillus mesentericus PB, the strain Bac.mesentericus PB sp. VNII PrB is used, deposited with the number 1755, "cultivated in a medium containing potato starch, corn flour, corn extract, and the culture Endomycopsis fibuligera Dekker is used as a producer of glucoamylase, which is cultivated in a medium containing starch, soy flour, corn extract NH^ PO^ and KH^ PO4.

Description

- 1 - 218 837 KSΠΚΟΑΗΜΕ KSOBPETEHKfl 3βΛΒΛββθ 0.9.12.77 2548551 / 28-13 Μ.Κλ. C 12 C 7/04 BδρβΤβΗΜΗ: Τ.Μ.ΠρΟΟΒβΤΟΒβ, Ε · Α · ΛΐΧ · βΛΐΧβΤΟΒ8, Β.JI.ΠρΟΒβΗΚΟ, Η.Η.Bacaxbe> a, B.Μ.ΗβχκβΗΟΒΚΚ, Β.Α.ΥοτκΗ-ΗΜΚΟΒ , Β.Φ.ΠΙθΜρΜΗ, C .Η .ΚβρΜΗβΒ, CB -Κ-xosa, Α.Η. Jlaeapasa η JI .Κοροηοβθ 3βΗΒΗΤβΛ &: BceC0X> 8HUft HayKHO — ΗΟΟΛΘΑΟΒβΤΘΛΒΟΚΚϋ MHCTMTyT npo-AyKTOB όρΟΧβΗΜΗ

CnOOOB OCAXAPMBAHMfl KPAXHAJIMCTOrO CHPbfl ΡΜΡΜΡΗΗΗΗΒϋϋΤΒΤΒΤΒΤΒΤΒΤΒΤΒΜΜΜΜρρρTHTHTHTHTHTHTHTHTHTHCT CTΟCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT

MaeecTeH cnocoG ocaxapaBanaa KpaxuaxbHoro cupta πρκ προκβΒΟΛ-CTBe CHMpTa nyTsu BHeceHMg β Hero KyxbTypH-npo ^ yueHTa tL -aiiMJiasK mb conceived Bacillus with cueca c KyxbTypoft-npo / tyueKTOM ΓΛ »ΚΟΘΜΚΛβ8Ν / 1 /.

Hey ^ oct machines μθβθοτηθγο cnocoGa ηβληθτοη λλητολβηοοτβ προ not c-ca ocaxapKBaHMH and cGpaxaBaHKji, τβκ XAX McnoxbeyeMNe xyxbTypuo6jtajta »T ΗβΛοστβτοκΗοϋ θκτμβηοοτββ and * Raxx Goxbmoit paexejgocaxapaBa» ayxbTyp max.

Ilexbx) xοδρβτβΒΚχ ABxaeTca ΒΗτβΗοκφΜκβιριβ nponecca ocaxapM-aaHKH and cCpaxHBaHMH and coKpamenae pacxojta ocaxapaBaxnqax icyxb-Typ. 3to jiocTaraeTca τβΜ, πτο b npejtxaraeuoM cnocoGa kb ^ and Bacillus acnoxbayBT orraMU jtpoxxeft Bacillus Sp menesteri- cus IMIMB ΒΗΗΗΠρΒ ® 1559-b sucks KyjibTypaabHoft mmjjkocth ab KauecTBe KyjihTypu - npcjtypeHTa rxxacoattaxaau - MRAM Λροκ-xeft Endontycopsisfibulegerá Dekker * - 21 · 6 cooTHomeHBa οοοτΒβτοτΒβΚΗΟ - 8 gjx / μλ λλ μ μ 1,0 - 1,5θλ / μλ AC Ha 1 r Kpaxuaaa. 2 218 857 ΠΙτβΜΜ-προΛγϊίβΗτ th · '- auujiasH ΒΐιρβπρίΒβΒτ Ba cpefle, cojxepxa-meft κβρτοφβΛΒΗΝδ Kpaxiiazr, xyxypyaHy »Myxy, xyxypysHHft axcTpaxT, ^ ByesMeineHHHfi φοοφβτ smmohmh h κβρδοΗβτ KaUbliMH. npeejraraeuuS cnocoó ocymecTBXHeTCH cjiejiyKaqMM oOpasoM.

PaaBspeHHyE KpaxMasocoflepxamy® uaccy oxjisama »T no TeMnepaTy-pn 55-60 ° C m aa, na» T s Hee CMecb xyxbBacillus type menestericusPβ Sp BHHHIIpB JE ΙΜΒΠΙΜΒ-1559 h Endomicopsis fibuligera / Lindner / Dekker -21 with cooTHoraeHHH coot- ΒβτοτββΗΗΟ 6- 8 θλ / μλ ΓλΑ m 1,0-1,5 efl / its AC Ha 1 r xpaaaiajia.OcaxapxBaHMe ocyiqecTBXHeTCH β τβπβΗΜβ 15-20 MMHyT; IIÍTaMM Bacillus menestericus ΠΒ Sp ΒΗΜΜΠρΒ, N? -ΊΜΠΜ B- 1559 nojtyneH with pesyxbTaTe 8θδορβτορΗοδ M8MeHHMBocTM πρκ λλμ-ΤβΛΒΗΟΜ ΗΜΗρβΗβΗΜΗ HS ΠΗΤΘΤβΛΒΗΟΗ Ορβββ, COCTOíUqeft Η3 ΚβρΤΟφβΛΗΜ φΗΒΗΟΧΟΓΚΜβΟΚΟΓΟ ρβΟΤΒΟρβ. Π h oč h h h h j j j j j j j oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc. HaKÓoabinaH axTHBHOCTb npx xyjibTMBMpoBaHMH πρκ 40 ° CflOCTwraeT 70 θα / μλ / θχτηβηοοτβ φβρκβΗτβ onpeAeHHJiacb no iie-Tony Α.Π. PyxxnAeBofi η Μ.Γ. «Ορ «ηβΒθϋ /.

KyabTypa Bacillus menestericus ΠΒ BHHWIIpB otho-chtch x rpynne ββκτβρΜϋ cennofi κ καρτοφΘΛΗΗο ^ · naao ^ eK. ΠΙτθμμ nueeT cjleAyonym xapaxTepMCTMxy. PορφοΛΟmne cxze npMSHaxK · Πθλοηχη paswtepoM 4-5 mxx 0.8-1, Omk, HHor.ua oÓpasyonHe nenon-kk H8 2-4 najtonex · OflHOOÓpaa oxpameHHHe, 6es xancyji, HenoA-ΗΗκχΗΗβ, oxpacxa no Tpaiey nojJoxwrejibHaa, oxpamHBamTCH paaCas-Λ6ΗΗΗΜ paCTBOpOM ΗΟΒΟΚβΤΜΛβΗΟΒΟΚ CMHHM ΗΛΗ Ηβ & ΤρβΛΒρΟΤΟΜ.ΠροτοιυιβθΜβ oxpamMBaeTCs oahopoaho. 3 - 218,857

BaxTepxx cnopooCpaaynnax ·

Poct xyjtbTypu hs nxTaTejBbHiix cpeflax.

Ha χβρτοφβΛΚΗΟΜ araparape xyxbTypa BupacTaeT κολοηηχμμ ok-pyrjtoft φοριοι; χολοηχμ no ρεβτγ Cejtue xxx TycKxo-Čexxe.IIOBepXHOCTb KOXOHUft ΙΠβρΟΧΟΒβΤββ, IjeHTp BHnyRXHIt. HA ΑΠΑ-κολοηκμ Óexue hih Tycxxo-ČexHe, oxpyraofi φορΜΗ, ΛΜΘΜβΤΡ 2-2,5 ιιχ;

Ha ΜΠΒ xyxbTypa Βκρβοτββτ ΟβΛοϋ οκλβΛ ^ βτοίί ΠΛβΗΚοϋ. BosmoxhoOAHopoflHoe κχκ xjionbeBHUHoe nOlfyTBeHXe ·

AnxaHxe aapoCHoe. <J> epMeHTHpyeT 6ea ΒκΛθΛβΗΗΗ rasa: rviroxoey, MaxbToay, caxapo-ay, Kcswoey, icaHHHT, coptXT.

HcnoabayeT ηχτρβτ.

KpaxMfiui paaxxxaei * ·

KaaexH rxupoxxayeT, ΑηβτρυϊΜβτΜΛΚθρόΗΗΟΛ oÓpaayeT.

On * rxuaxbBaa TexnepaTypaTa nxa δχοοχΗτβββ Z -axiaiaau40-42 ° C. MKHHiiaxbHafl TeMnepaTyp 20 ° C, MaccxuaxbHax-50 ° C. ΠβΤΟΓβΗΗΗΜΚ H CβCKHMH CBO &amp; CTB81ÍX Ηβ ΟβΛΘΑΘβΤ.

KyxbTMBxpoBaHxe menestericus ΠΒ Bacillus Sp ΒΗΗΗΠρΒ nponyueHTa -βΜχχββχ - ocymecTBxx »T Ha cpejje b κοτοροϋb xaxecTBe κοτοηηχκθ yrxepo ^ and xcnoxbayDT χβρτοφβχ bniili xpax Keep-X KyxypyaHy» xyxy and x xxHepaxbHiix coxeft - AByxaaiie-meHHHÍl φοοφβτ 8MUOHHH X ΚβρβΟΗβΤ ΚβΛΒΙξΜΛ · HcTOHRHKOM < 5X0X0-rxxecxx θκτχβκηχ BemecTB ηβληθτοη xyxypyaHuft Βκοτρβχτ, xxxOBK, ΜΛΗ KOpUOBlie APOXXM · ΠροηβΗΤΗοβ cooTHoraeHxe ηηγρθαμθητοβ cpenH% i »ΧβρΤΟφβΛΒΗΗϋ KpaXMaJIxyxypyaiiax icyxa / KH4 / 2hpo4CaCO ^ 0.25-0 1.0-1.2 1.5-1.7 , 30 0.20-0-22 4,218,857

xyxypyaKuK 8κοτρβκτOBK

ApOXKM ,20ρΜΟΒΗΘΒΟΛβ 0,20-0,22 3,50-4,00 4,00-4,10 OCT & KbHOe Endomycopsis fibuligera / Lindner / Dekker -21-npoAyueHT γλρκοβμιιχθ ^ η - suieeT cxeflyWRy® xapaxTepHCTMxy:

Ha RUSK cycjie - ΤΜβΠΤΜρΟΒβΗΗΗϋ MMUeJXHft, ββΗβΗΜΤβΛΒΗΟβ HMC-xo noHKyraqitx χροχχβΒυχ κχβτοκ oaaxbKoft φορκκ paauepaMH 9-10xx 5-6 mru. Ha cycxo-arape koxohmh Oexasavue, nymMCTue, aunyx-Λϊΐβ, C pOBHHMM ΚρβΗΜΜ. ΟβρβΧΜΒΘΘΤ HUDKOSy, CaXapO3y, ΜβΛΒΤΟ-ey, ρβφΗΗοβγ- cxaGo, raxaxToay x xaxTOsy He cĎpaxKsaeT.

Acc wmhji xpyeT mho my caxapa, xpaxuax, Bsicmxe οπκρτΗ, opraHH-ΠβΚΗβ KMCXOTH. Accκτρβτκ He accwMJWHpyeT, ap6y? KH He pacmenxaeT, b cpefle SesΒΗΤΘΜΖΗΟΒ He ρβΟΤβΤ. % &amp; &amp; UaKOOXeBItil ΚΟ »H BMHX Θ8Η flSHHhlM arrau-mom ncnojxbsyeTca cpe« to cxeAyRiRero cocTaea,% ·. pacTBopmeuftKpaxMax-1.6 ^ 1 coeBafl uyica-8; xyxypyaHuft βχοτρβχτ -4; φοοφορ-koxmcxsiS Kanuli -0.06; OxHosaueiqeHHMfi φοοφορΗΟΚΗΟΛΗϋ auuo-HHft -0.16. npHMepu ocymecTBxeHHH cnocoča. npHuep N? 1.

PasBapeHHy »uaccy oxxaxAaxK λο TeunepaTypu 55®C η saflaBeaxb Hee cuecb xyxbType Endomjrcopsis fibuligera Dekker-21, AeHCKBispoBaHHKft b ΗΒΦΜ AH CCCP πολ N? Y744, x Bacillus menestericus ΒΒ ΒΗΜΜ Β Β Β 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 Α Α Α Α Α Α ΑΓ Endomycopsis fibuligera Dekker -21 BHpSQV <B8JIK HS Ορβ ^ β ΟΛβΛΥ ΙΚβΓΟ COCTSBa / b% / ΚρβΧΜβΧ-1,6; coeBafl uyxa -8; xyxypysRuft axcTpaRT -4; KHg P04 -0.06; NE4H2PO4-0.16. Bacillus menestericus ΠΒ Sp ΒΗΗΗΠρΒ

BupanptBaftM Ha cpexe cxenywjero cocTaBa / b sec.% /: Xpaxuax xap-τοφβΛΒΗΗϋ -1.0; uyxa Kyxypy8Hax-1,5} (WH4) 2 ΗΠ04 - 0,25; 5,218,857

CaCO 3 - 0.20; KyKypyeHuft θκοτρβκτ -0,2; βολ® -οοτθλβηοθ.

Pacxofl xyjifeTypaxbBoft χηλκοοτη ηθ ocaxapHBaHHe Dacillusmenesterikus ΒΒΒΗΜΜΒΗΜΜΒΗΜΜΒΒΗΜΜΒΗΜΜΒΗΜΜ5555555555555555555555555555555555555555555555555555555555555. Un ο un un un un un un un un un un un un un un ee ee char char char char un un un un un un ee ee ee ee 1 1 1 1 1 1 xjie-tok. Προpece cepaacMBaHHH ocyujecTBXHeTcs πρκ TeuiiepaType 28-30 ° CB ΤβΗβΗΗβ 60 naCOB. ΠρΚΜβρ 2.

TexHOxo γήπθckκβ onepaiptu ocymecTBxnxH 8Ηβχογηπηο πρκΜβρa 1 τολβκο b nMTaTeJJbHy »CPEx XXh βηρθιηηβθηηη Bacillus menestericus ΠΒ ΒΗΜΜΠρΒ saxasexn bmccto KyicypysHoro skct- ρβκτβ - oÓorameHHuit óexKOBuií κοΗπθΗτρβτ b κοχΗΗβοτΒβ 4% .Pacxox xyjibTypHoii xhxkocth menestericus ΠΒ Bacillus Sp ΒΗΜΜΠρΒ c aKTHBHOCTbro 50 ex / ux cocTaBHx 0 , 03 μλ, and Éndomycopsis fibuligera Dekker 21 - 0.3 μλ Ha 1 r KpaxMajia.

Takhte oÓpaaoM χθηηηθ ποκΜβρυ He HcnepnMBaxzr bosmoxhocthπρβχχaraeuoro cnecoda him πκββΗΒβυτ MTO πιτθμμ βκχβ Baci llus-menestericus ΠΒ Sp ΒΗΜΜΠρΒ 1755 Ο0ΧβχθβΤ ΚΟΜΠΧΘΚΟΟΜ aMHXOXHTHHeCKHX ΠρΟΤβΟΧΗΤΗΗβΟΚΗΧφβρΜβΗτοΒ, ΗβοόχοΛΗΜΗΧ xa rxyCoKoro ocaxapHBaHHH KpaxMaxnc-ΤΟΓΟ CHpbfl.

CnOCOHHOCTb ΚΟΜΠΛβΚΟβ βΜΗΧΚΤΗΗΘΟΚΗΧ φβρΜβΗΤΟΒ HHCTpO ΟΛΗΟΓΗ ΠΟΧΗχρΟΧΗβΟΒΘΤΒ ΚρβΧΜβΛ ΛΟβρβΧΗΒΘβΜΗΧ ΟβΧβρΟΒ, and ΛΠΛβΚΟβΒ ΤβΗβΗΗβ 20 ΜΗΗ, ρΗ ΛΗρβΛΗ ΟΟΟΤβΒΧΗΘΤ 5,2 + 5,0.

OcaxapeHHan UACC oxxaxxaxacb χο TeeneparypM 30 ° C προτβο-XHTHUeCKMX φβρΜβΗΤΟΒ K8T8XHSMpOBOTb ΓΚχρθΧΗ9 ββΧΚΟΒ Η ΠβΠ-ΤΗχΟΒ ΧΟ SMHHOKHCXOT χβΧββΤ χβΗΗκίί ΒΗχ Η ΙΠΤβΜΜ ΙξβΗΗΜΜ RXXΠρ0Μ3Β0Χ0ΤΒΘ CnHpTa, ΤΘΚ ΚβΚ CnOCOÓCTByeT ΗΗΤβΗΟκφΗΚβΠΗΗπρο PE approx ocaxapHBaHHH, προκσχοχππξθΓΟ ποχ χβκοτΒΗβΜ κομπλθκοθφβρίίβΗΤΟΒ. 6 218 857 ΠρΟΤβΟΛΧΤΡΠίβΟΚΘΗ aKTXBHOCTb ΙΠΤΘΜΜβ 1,6-1,7 Θλ / μΛ CHOCOÓCTByeTΗβκοπΛβΗχκ β npoixecce cnzpTOBoro βaxeHxx β cpe ^ e ημχηοκηολοτ, HeoCxo ^ MMwx AJia ycKopeHHH οροχβΓβΗβρβχχχ, for aKTXBxsauxx apox-xeK.

BCE 9 to b c coBOKynHOCTM BHCOKoaKTMBHKM nrraMMOM Endomycopsisfibuligera Dekker -21 oóecneHXBaeT τβΗοχφχκβΗΗχ npoueccaocaxapwBaHMH x x cCpaxxeaHXH, coxpamaeT pacxofl ocaxapxBarmxxKyjibTyp, κροΜθ τογο, flOCTxraeTcx 6oaee rmyóoicoe cópaxxBaHxenojTMcaxapj-iflOB, κ cHxxeHxe what, qepxaHKa HeoÓpaxeHHux caxapos bČpaxxe.

ΦΟΡΜΥΤΙΑ M30BPETEHMH first Cnocos ΟΟΘΧβρΧΒβΗΗΧ ΚρβΧΜΘΛΜΟΤΟΓΟ CHpbH npx npOM3BO, HCTBe cnxpTa rivet BHeceHxa b © KyjibTypH Her-NPO-yiíeHTa -aaixjiasNxa pofla CMecx Bacillus b-c KyjibTypo npo.nyu.eHTOM γλβκοθμμ-ΛΒ8Η, ΟΤΛΜΠΘΒΠρίΖΟΗ ΤβΜ, HTO, C ΙξβΛΒΚ ΧΗΤβΗΟΧφΧΚβΗΧΧ ΠρΟΙξθΟΟβocaxapMBaHXH x copaxxBaHxa x coxpaHeHXH pacxo ^ and ocaxapxBasiqxxxyjtbTyp, X8 poaa Bacillus xcnojibeynT ιπτθμμ upoxxefi

Bgcillus menestericus ΠΒ Sp ΒΠρΒ N? UMMM B-1559 b x ^ g xyjibTypH-nponyieeHTa ΓΛ®κοθΜΧΛβ8Η-πιτβΜΜ ApoxxeSEndomycopsisfibuligera Dekker - 21 b cooTHomeHxx x οοοτΒβτοτΒβΗΗΟ 6-8 ej mji ΓλΑ x 1,0-1,5 efi / uji KpaxMana. Second CnocoO well A.M., OTJixxaBmxftcx τθμ, ητο niTaMM-npoflyueHT-AMX-jjaeu BupamxBaDT CPE Ha ^ e ^ what epxameS κβρτοφβΛΒΗυίί xpaxMaa, KyKypyaHy »iiyxy, KyKypysHiift βκοτρβκτ, AByxeaMeineHHHft φοοφοτaMMOHXH x KapĎOHaT KanbxxH. -7 - 218,857 AHHOTAUKfl

Cnocoó OCaxapKBaHMH ΚρβΧΜβΛΗΟΤΟΓΟ CHphH πρκ npOH8BOACTBecnxpTa ΉβΟβρβΤβΗΚΘ OTHOCHTCH X ΟΠΚρΤΘΒΟΚ προΜΗπυιβΗΗΟΟτκ. C ^ qeJtBB ΜΗΤβΗΟΜφΚΚβίχΗΗ ΠρΟΙ βΟΟΟΒ OCaxapMB8BHH Her ΟΟρβΧΙίΒβΗΗΗh coKpamemte pacxofla ocaxapjtBaraiKx ΜβτβρκβΛΟΒ ocaxapBBaMiieKpaxxaxHCToro cupbH ocymecTBXHeTCH cuecbn Endomycopeia fibuligera Dekker -21-npOAyneH * and ΓΛΚΧΟβΜΗΛββ »h ÓaKTepMaxbHoft xyxbTypu of Bacillus menestericusΠΒ - ΠρΟΛΥΐΧβΗΤΘ -8MJUI88H · Z B 6-8 ΟΟΟΤΗΟΙΠβΗΜΜ Qr / MX ΓλΑ H1 , 0 - 1.5 br / mx AC Ha 1 r xpaxiiajsa ·

Ha BJwe Bacillus menestericus I® MCnexbeyiOT iqtqmm

Bacillus menestericus ΠΒ Sp ΒΗΗΗΠρΒ

AenoHKpoBaHHuft no ® 1755, sup amin aemift Ha cpaas, coAepxa-meft β β p p p xpaxuaa, xyxypyaHyloxy, xyxypyasHft β ο-KT KT KT KT, (WH4) g HP04 h CaCOg. B xatiecTBe προΑΥΠβΗτβ xjujxoaifJuiaaH Mcnoxbeypr xyxbTypes Eudomj copsis fibuligera Dekker> BHpam ^ HBaeMym Ha cpefle, coAepxamefi xpaxM ^ t, coeeyn xyxy, xyxypyaHHft βχοτρβχτ NH4HgPO4 her KHgPO ^

Claims (2)

BACKGROUND OF THE INVENTION 1 A process for the saccharification of a starch raw material in the manufacture of amylase-producing liqueur from the genus Bacillus, in general, with a gldcoamylase-producing culture, characterized in that a process for saccharification and fermentation is used to intensify and reduce the consumption of saccharification-contributing cultures. strain Bacillus mesentricus PB Sc. VNIXPrB # CMIM V-1559 in a liquid culture medium and as a glucoamylase-producing culture uses Endomycopsis fibuligeraDekker-21 yeast strain at a ratio of 6 to 8 units / mlGla and 1.0 to 1.5 units / ml AS per 1 g of starch . 2. A process according to claim 1, wherein the amylase-producing strain is cultured in an environment comprising potato starch, corn flour, corn extract, ammonium hydrogen phosphate and calcium carbonate. made by the USSR's Committee on Inventions and Discoveries, Moscow, SU.