ES8101618A1 - Microorganisms for the production of microbial beta- galactosidase, microbial beta- galactosidase, method for production thereof and application thereof - Google Patents

Microorganisms for the production of microbial beta- galactosidase, microbial beta- galactosidase, method for production thereof and application thereof

Info

Publication number
ES8101618A1
ES8101618A1 ES487503A ES487503A ES8101618A1 ES 8101618 A1 ES8101618 A1 ES 8101618A1 ES 487503 A ES487503 A ES 487503A ES 487503 A ES487503 A ES 487503A ES 8101618 A1 ES8101618 A1 ES 8101618A1
Authority
ES
Spain
Prior art keywords
galactosidase
beta
lactose
production
hydrolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES487503A
Other languages
Spanish (es)
Other versions
ES487503A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Industri AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK10379A external-priority patent/DK10379A/en
Application filed by Novo Industri AS filed Critical Novo Industri AS
Publication of ES8101618A1 publication Critical patent/ES8101618A1/en
Publication of ES487503A0 publication Critical patent/ES487503A0/en
Granted legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • A23C21/023—Lactose hydrolysing enzymes, e.g. lactase, B-galactosidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A beta-galactosidase product which in regard to enzyme chemical and immunological properties is or is substantially identical to the beta- galactosidase which is produced by fermentation of the microorganism NRRL B-11,229, is produced by means of a microorganism belonging to the taxonomic group which is represented by the type culture NRRL B-11,229. The microorganism NRRL B-11,229 itself seems to be related to Bacillus stearothermophilus. The beta-galactosidase has a molecular weight of the order of 116,000 determined by gel electrophoresis. It has a high thermostability and a low inhibition from lactose or hydrolysis products originating from lactose. The beta-galactosidase product - if desired as an immobilized preparation - may be used for hydrolysis of a lactose containing liquid, for example whey, in order to form a syrup containing glucose and galactose and a minor amount of lactose. The hydrolysis may be performed continuously or batchwise.
ES487503A 1979-01-10 1980-01-07 A METHOD FOR THE MANUFACTURE OF A B-GALACTO SIDASA MICROBIAL ENZYMATIC PRODUCT. Granted ES487503A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK10379A DK10379A (en) 1979-01-10 1979-01-10 MICROBIAL BETA-GALACTOSIDASE MICROORGANISM FOR PREPARING ITS PROCEDURE FOR PREPARING IT AND USING THEREOF
DK519079A DK146325C (en) 1979-01-10 1979-12-06 BETA-GALACTOSIDASE, METHOD OF PREPARING IT AND USING THEREOF

Publications (2)

Publication Number Publication Date
ES8101618A1 true ES8101618A1 (en) 1980-12-16
ES487503A0 ES487503A0 (en) 1980-12-16

Family

ID=26063306

Family Applications (1)

Application Number Title Priority Date Filing Date
ES487503A Granted ES487503A0 (en) 1979-01-10 1980-01-07 A METHOD FOR THE MANUFACTURE OF A B-GALACTO SIDASA MICROBIAL ENZYMATIC PRODUCT.

Country Status (9)

Country Link
AU (1) AU5438480A (en)
DE (1) DE3000598A1 (en)
DK (1) DK146325C (en)
ES (1) ES487503A0 (en)
FR (1) FR2453214A1 (en)
GB (1) GB2038837A (en)
IT (1) IT8047556A0 (en)
NL (1) NL8000033A (en)
NZ (1) NZ192552A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3811964A1 (en) * 1988-04-11 1989-10-19 Biodyn Ag Beverage for human nutrition
FR2749307B1 (en) * 1996-05-29 1998-09-04 Roquette Freres PROCESS FOR THE PREPARATION OF D-ARABITOL

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1493542A (en) * 1974-12-04 1977-11-30 Reynolds Tobacco Co R Production of thermostable lactose
NZ190603A (en) * 1978-06-07 1982-03-23 Nat Res Dev Heat-stable -galactosidase derived from bacillus stearothermophilus hydrolysis of lactose

Also Published As

Publication number Publication date
DK146325B (en) 1983-09-05
DK519079A (en) 1980-07-11
AU5438480A (en) 1980-07-17
FR2453214A1 (en) 1980-10-31
GB2038837A (en) 1980-07-30
ES487503A0 (en) 1980-12-16
NZ192552A (en) 1982-03-30
DK146325C (en) 1984-02-20
IT8047556A0 (en) 1980-01-09
DE3000598A1 (en) 1980-07-31
NL8000033A (en) 1980-07-14

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19971001