CS215924B1 - Device for automatic recording the elution volume by the column fluid chromatography - Google Patents
Device for automatic recording the elution volume by the column fluid chromatography Download PDFInfo
- Publication number
- CS215924B1 CS215924B1 CS24080A CS24080A CS215924B1 CS 215924 B1 CS215924 B1 CS 215924B1 CS 24080 A CS24080 A CS 24080A CS 24080 A CS24080 A CS 24080A CS 215924 B1 CS215924 B1 CS 215924B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- siphon
- elution volume
- automatic recording
- fluid chromatography
- column fluid
- Prior art date
Links
- 238000010828 elution Methods 0.000 title claims description 8
- 238000004587 chromatography analysis Methods 0.000 title claims 2
- 239000012530 fluid Substances 0.000 title 1
- 239000007788 liquid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 238000004811 liquid chromatography Methods 0.000 description 2
- 101100313377 Caenorhabditis elegans stip-1 gene Proteins 0.000 description 1
- 101100313382 Dictyostelium discoideum stip-2 gene Proteins 0.000 description 1
- 101100516335 Rattus norvegicus Necab1 gene Proteins 0.000 description 1
- 101150059016 TFIP11 gene Proteins 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Description
Predmetom vynálezu je zariadenie na automatická zaznamenávanie elučného objemu pri stípoovej kvapalinovej chromatografií.SUMMARY OF THE INVENTION The present invention relates to an apparatus for automatically recording the elution volume in a column chromatography.
Doteraz používané zariadenia na meranie elučného objemu sú založené na principe sifonu, alebo počítania kvapočok. Pri meraní elučného objemu na principe jedného sifonu sa sluent vytskajúci z chromatografickej kolony po přechode dstektorom zachytává v sifone s presne známým objemom a každé pretečenle sifonu sa zaregistruje zapisovačom. Přitom je obtiažna signalizáoia pretečenia sifonu na zapisovač. Všetky doteraz používané zariadenia vyžadujú zložité a výrobně náročné elektronické sústavy.The elution volume measuring devices used so far are based on the principle of siphon or droplet counting. When measuring the elution volume on the principle of a single siphon, the agent emerging from the chromatographic column after passing through the dstector is collected in a siphon with a precisely known volume and each overflow of the siphon is registered with a recorder. At the same time, it is difficult to signal the flow of the siphon onto the recorder. All the devices used so far require complex and production-intensive electronic systems.
Uvedené nedostatky odstraňuje zariadenie podlfe vynálezu, ktorého podstata spočívá v tom, že má nad sebou umiestnené dva sifony, z ktorých spodný je upevněný na výkyvnéj pake so závažím a prvým kontaktem umiestneným nad druhým kontaktom, pomocou ktorého sa pri každom přetečení objemu sifonu preruší na zlomok sekundy elektrický prúd privádzaný z detektora do zaplsovača.The above-mentioned drawbacks are eliminated by the device according to the invention, which consists of having two siphons placed on top of each other, the bottom of which is mounted on a swinging paw with a weight and a first contact placed above the second contact. seconds the electrical current fed from the detector to the recorder.
Vynález automatického zaznamenávanie elučného objemu pri stípoovej kvapalinovej ohromatografii založený na principe dvooh sifónov je přesný konštrukčne jednoduchší a lačnější ako doslal* používané zariadenia.The invention of automatic recording of the elution volume in Stip Liquid Hydromatography based on the principle of two-way siphon is precise in construction simpler and more costly than conventional devices.
Zariadenie na automatické zaznamenávanie elučného objemu pri stípoovej kvapalinovej ohromatografii je znázorněné na pripojenom obrázku. Skládá sa z dvoch sifónov, spodnéhoAn apparatus for automatically recording the elution volume in a staged liquid chromatography is shown in the attached figure. It consists of two siphons, the bottom
215 924215 924
215 924 sifóna £ a druhého pohyblivého sifóna 2 připevněného na výkyvnéj páke £. Na druhom konci paty 2. de P^vý kontakt 2» ktorý po oddialení od druhého kontaktu 6 přerušuje přívod elektriokého prúdu od detektora k zapisovačů. Nulová poloha paty £ sa vyvažuje závažím £.215 924 of the siphon 6 and the second movable siphon 2 mounted on the pivot lever 6. At the other end of the foot 2 d L e ^ 2 Elevator Contact »is the delay of the second contact 6 elektriokého cuts off the current from the detector to a recorder. The zero position of the heel £ is balanced by the weight £.
Pri prázdnota pohyblivom sifone 2. je paka £ nakloněná tak, že elektrické kontakty £ a £ dosadajú na seba a elektrický obvod medzi detektorom a zapisovačom je spojený. Do pevného sifonu £ s presne vymedzeným objemom pomaly přitéká elučná kvapalina. Keď sa spodný sifon £ naplní, pretečie obsah náplně do pohyblivého sifóna 2 v dosledku čoho sa paka £ převáži a rozpojí kontakty £ a 6. Náplň sifonu 2 okamžité pretečie do zbernej nádobky a paka sa převáži do povodnej polohy. Toto krátkodobé prerušenie elektrického obvodu do zapisovače sa prejaví na registračnom papieri ako signál. Posuvné závažie £ sa nastaví tak, aby bola doba preklopenia páky čo najkratšia a potenciometrom £ sa nastaví výška signálu registrovaného zapisovačom. Podmienkou spolfehlivej činnosti zariadenia je správné nastaveni? závažia £, pričom musí byť objem pohyblivého sifóna £ menší ako pevného sifóna £.In the emptiness of the movable siphon 2, the pawl 6 is inclined such that the electrical contacts 4 and 6 contact each other and the electrical circuit between the detector and the recorder is connected. An elution liquid slowly flows into the solid siphon 6 with a precisely defined volume. When the bottom siphon 6 is filled, the contents of the cartridge flow into the movable siphon 2 as a result of which the pack 6 is then transported and the contacts 6 and 6 are opened. The siphon filling immediately flows into the collection container and then transports to the flood position. This brief interruption of the electrical circuit to the recorder is reflected on the registration paper as a signal. The sliding weight sa is adjusted so that the lever flipping time is as short as possible and the height of the signal registered by the recorder is adjusted by the potentiometer.. The condition of reliable operation of the device is correct setting? weight £, the volume of the movable siphon menší must be smaller than the fixed siphon..
Vynález možno využit’ pri kvapalinovej chromatografii v laboratóriaoh.The invention can be used in liquid chromatography in a laboratory.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS24080A CS215924B1 (en) | 1980-01-11 | 1980-01-11 | Device for automatic recording the elution volume by the column fluid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS24080A CS215924B1 (en) | 1980-01-11 | 1980-01-11 | Device for automatic recording the elution volume by the column fluid chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS215924B1 true CS215924B1 (en) | 1982-10-29 |
Family
ID=5334179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS24080A CS215924B1 (en) | 1980-01-11 | 1980-01-11 | Device for automatic recording the elution volume by the column fluid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS215924B1 (en) |
-
1980
- 1980-01-11 CS CS24080A patent/CS215924B1/en unknown
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