CS196474B1 - Method of producing proteinous concentrate from micro-organisms cell mass - Google Patents

Description

Another effective extraction agent is carbamide, but it is necessary to use concentrated solutions in which poisonous cyanates are readily formed, and carbamide must be separated from proteins by lengthy dialysis, while regenerating the reagent from very dilute solutions.

188 474 flows after dialysis are uneconomical.

These drawbacks are overcome by the process for the production of protein concentrates from the cell mass of microorganisms according to the invention, characterized in that the cell biomass is leached with an acid solution at pH 4.0 to 4.25, after which the euepension is separated from the solution and 100 up to 200 g of CaO and HgO slurry which is macerated for 12 to 24 h at 30 to 60 ° C, after which the solution is saturated with carbon dioxide gas until the pH of the solution reaches 6.5 to 7.5, It is filtered and the filtrate obtained is dried and processed to a protein concentrate.

In the treatment of cell mass by acid, some non-proteinaceous biomass fractions are eliminated and residues of the recultivation medium are removed. In addition, some proteins are rehydrated, especially when using a eject dried material. The addition of a mixture of CaO and HgO leads to protein solvation, some ee lipids are converted to insoluble calcium soaps, while contamination in the cell mass is reduced. Saturation of the solution with carbon dioxide gas precipitates excess calcium hydroxide in the form of carbonate and adsorbs dark melanoid residues into the precipitate. At the same time, fine suspended particles are entrained into the precipitate so that a clear solution of the proteins is obtained by filtration. It is either dried directly or the proteins precipitate at the isoelectric point, separated again, neutralized and dried to a purified concentrate.

This method of protein concentrate production has the following advantages over the present methods: costly cell disintegration can be avoided, unsuitable substances are removed before protein extraction, partial rehydrataoi of proteins occurs, contaminants cannot multiply during extraction, some lipids bind to sediment, protein solution ee easily separates from ballast, dark products are formed in the process in small quantities, protein solution can be processed for some applications without renumbering.

yields are significantly higher than those of similar processes, the protein isolation waste contains harmless calcium carbonate and is therefore renderable.

Further, the invention is illustrated by the following non-limiting examples.

Example 1 - Extraction from dried biomass A portion of the dried biomass of Hicroooecus varlans was resuspended in 10 parts of water heated to + 60 ° C. Dilute sulfuric acid was added until the pH of the suspension dropped to 4.2. Stir 1 hour, centrifuged, sediment left for 6 hours at +3 ° C. Then 0.15 parts of CaO with a small amount of water is mixed into the sadiman. Leave in a thermostat overnight at +45 ° C, then make up to the original volume with hot water and stir for 1 hour. Bubble with tas 474 carbon dioxide until the pH has dropped to 7.0. Centrifuged to obtain 7 parts of a clear solution which was freeze-dried. Yield: 0.33 parts of dry concentrate containing 75% crude protein.

Example 2 - Extraction from native liters of liters of a concentrated suspension of Baolllus megaterlum heated to +60 ° C and acidified with sulfuric acid to pH 4.25. The sediment was mixed with 150 g of CaO and stirred for 24 hours at + 37 ° C. Then make up to 10 l with water for 1 hour and stir at + 80 ° C. Bubbled through with carbon dioxide to pH 7.5. Centrifuged, the eupernatant was acidified with hydrochloric acid to pH > 3.5, left in the refrigerator overnight. Centrifuged again, the sediment was neutralized with sodium hydroxide and dried in vacuo. Yield - 250 g of concentrate with 82% crude protein.

Example 3 - Seaweed Extraction kg paste resulting from the extraction of bloatmulifiers from the extracted biomass of Chlorella vulgaria was mixed with a small amount of water to a thick slurry and acidified with sulfuric acid to pH = 4 and left in the refrigerator overnight at +3 ° C. . Then mixed with 200 g CaO and macerated for 12 hours at +50 ° C. Dilute with hot water to a volume of 10 liters and stir for 1 hour at + 75 ° C. Saturated with carbon dioxide to pH = 6.5, then filtered and the filtrate dried in a spray drier. Yield - 160 g protein concentrate containing 85% crude protein.

Claims (1)

PROCEED THE INVENTION Process for producing protein concentrates from the cell mass of microorganisms, characterized in that the cell biomass is leached with an acid solution at pH 4.0 to 4.25, after which the suspension is separated from the solution and the sediment obtained and 100 to 200 g of CaO and HgO suspension are macerated at 30-60 ° C, whereupon the solution is saturated with carbon dioxide gas until the pH of the solution reaches 6.5-7.5, is then filtered and the filtrate obtained is dried and processed to a protein concentrate.