CS19091A3 - Process for preparing probiotic preparations for people and animalsnutrition - Google Patents

Description

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BACKGROUND OF THE INVENTION The present invention relates to the preparation of probiotic preparations for nutrients and animals by producing probiotic preparations in dried form for human consumption, preferably in the form of tablets, and in addition, for the production of animals, the probiotic preparations can be prepared in liquid form. contain yeast biomass or hydrolysates or autolytes prepared therefrom, preferably for human nutrition from Saccharomyces cerevisiae, Saccharomyces carlsbengensis, Kluyveromyceslactis, Saccharomyces fragilis and Kluyveromyces marxianus, and animal feed from Candida and Torula feed together with bacteria capable of passage or colonization of the digestive tract of humans and animals such as lactobacillus acidophilus, Lactobacillus casei, Lactobacillusbulgaricus, preferably a mucilaginous strain, Streptococcus salivariussubsp. thermophilus, preferably a mucilaginous strain, Streptoco ccusfaecium, Bifidobacteria with the exception of Bifidobacterium dentium, preferably Bifidobacterium longum, bifidum, breve, infantias or adolescentis, and in addition, probiotics may contain additional bacteria of the genus Propiobacterium such as Propionbacterium shermanii and freudenreichii or Pediococcus acidilactici, wherein the bacterial cultures are prepared by cultivation in a milk-based medium, preferably skimmed milk, whey, buttermilk, milk, whey or buttermilk permeate, raw or purified lactose, the resulting probiotic preparations containing 2 to 98% yeast biomass or derivatives thereof such as hydrolysates or autolysates In addition, the probiotic preparation for human nutrition can be enriched with growth substances — 2 ** bifidobacteria, such as lactulose, plant growth substances contained in carrot and soybean dry matter, or enriched with sea buckthorn dry matter tlákovitý.

The invention further relates to the aforementioned production, wherein the probiotic pre-preparations are produced in a dried form without the addition of yeast biomass and their derivatives, with the exception of those components which can be used in the cultivation of bacculoskeletal cultures including yeast extract, where probiotic preparations they comprise the following combinations of bacterial cultures, yoghurt culture Lactobacillus bulgaricus or Streptococcus thermophilus and / or Lactobacillus acidophilus and Bifidobacteria, with the exception of Bifidobacterium dentium, and Pediococcus acidilactici, yogurt culture, Lactobacillus bulgaricus or Streptococcus thermophilus and Streptococcus faecium, or Lactobacillus acidophilus, respectively, or Bifidobacteria and Streptococcus faecium, or / yogurt culture, Lactobacillus bulgaricus or Streptococcus thermophilus, Lactobacillus 'acidophilus and / or Bifidobacteria,' Lactobacillus acidophilus and Streptococcus faecium, wherein the bifidobacteria itself is composed of strains in a symbiotic culture capable of growing under facultatively anaerobic and microaerophilic conditions, the resulting probiotic preparation contains 0 to 15% yeast biomass, its hydrolyzate, autolysate and / or extract in the dry matter of the finished product, with the bacteria being cultured in the medium whose basis it consists of milk raw materials, such as milk, preferably skimmed milk, whey, buttermilk or permeate prepared therefrom, raw or purified lactose, during manufacture, before or after starches and derivatives thereof such as heat-treated starch and maltodextrins according to both the production methods of 1 and 2, the content of which in the product may be up to 10%, preferably 0.5 to 5%.

The prior art processes for the preparation of probiotic preparations utilize pure culture in lyophilized form, such as pharmaceutical preparations containing bifidobacteria or Lactobacillus acidophilus, other pharmaceutical preparations in paste form containing, as growth additives, transoligosaccharides isolated from soybean or prepared by biotechnology. These transoligosaccharides are not yet available in the required amount in addition, the use of probiotics is based on preparations containing Streptococcus faecium for animal nutrition, without growth-additive, other whey-grown combinations with, for example, Lactobacillusacidophilus and propionibacteria without yeast biomass are not included in the disclosure. Drying of the bacterial cultures blanks in the described combinations, optionally with neutralization during manufacture or only before drying, ensures good survival of the microfloam present in the order of 10 to 107 in one gram of dry matter after production, normally 107 to 10 in 1 g of dry matter In the current industrial practice, lyophilization is still a costly process that limits the production of probiotic preparations for human diet and on animals. Common methods include the production of infant formula and baby food enriched with microorganisms such as bifidobacteria and Laktobacillusacidophilus. · The form of these products presupposes their recovery in boiled water and the preparation of baby food for infants and children.

In addition, dried infant formulas are not intended for routine distribution being allocated based on a prescription for infants.

Neither the taste characteristics nor the form correspond to the normal consumer habits of the adult population. Other additive substances which are growthifiers for bifidobacteria and lactobacilli cultures, such as yeast biomass and derivatives thereof such as autolysates, hydrolysates or extracts, especially at their high concentrations of 5 to 90% by weight, may also not be used in terms of taste properties. . in the final product. It is known to produce single dried brewery yeast or other yeast-based food products. These preparations themselves have undoubtedly a positive nutritional effect due to the content of vitamins, in particular Group B, the content of enzymes, nucleic acids and mineral substances in addition to substances of steric nature.

The disadvantage of these preparations is that, while they may promote the development of the original intestinal microflora, especially those with a positive effect on human health, they cannot restore the natural bacterial iterial balance in the gastrointestinal tract, for example after its disruption due to external factors. especially in the treatment of antibiotics or changes caused by diarrhea, the effects of ionizing radiation, improper nutrition, and stress and movement in outer space, as well as repeated x-ray or therapeutic irradiation, have a negative impact on natural equilibrium.

In the market, existing fermented dairy products containing passages-capable bacteria and digestive tract populations may not be acceptable to the entire population, and such products are not always available throughout the market. Also, at today's frequent travel, it is not possible to provide the products in everyday consumption.

For animals there are ready-to-feed foods with probiotic effects, mostly based on the culture of Streptococcus faecium or 4 in combination with propionbacteria or on the use of bifidobacteria and Lactobacillus acidophilus alone or on their combination. especially the fast-growth additives, especially bifidobacteria and lactobacilli after feeding

The yogurt bacteria themselves, i.e., Lactobacillus bulgaricus and Streptococcus salivarius sulsp thermophilus, especially in proto-cooperative symbiotic culture, exist in both lyophilized and dried form, but without the addition of yeast biomass and derivatives derived therefrom as described in the specification.

These drawbacks are eliminated by the process of making the probiotic preparations for human and animal nutrition of the invention such that probiotic preparations in dried form, preferably in the form of tablets, are produced for human consumption, and probiotic preparations can also be prepared in liquid form for animal nutrition. wherein the preparations comprise yeast biomass or hydrolysates or autolysates prepared therefrom, preferably for human nutrition from Saccharomyces cerevisiae, Saccharomyces carlsbengensis, Kluyveromyces lactis, Saccharomyces fragilis and Kluyveromyces marxianus, yeasts used in the feed of Candida and Torula, together with bacteria capable of passage or colonization of the digestive tract of humans and animals such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, preferably mucilaginous strain, Streptococcus salivarius subsp. thermophilus, preferably a mucilaginous strain, Streptococcus faecium, bifidobacteria with the exception of Bi-fidobacterium dentium, preferably Bifidobacterium longum, bifidum, breve, infantias or adolescentis, and in addition, propiotics may additionally contain Propionobacterium bacteria such as Propionbacteriura shermanii and freudenreichiinebo Pediococcus acidilactici, wherein the bacterial cultures are prepared by culturing in a milk-based medium, preferably skimmed milk, whey, buttermilk, milk permeate, syrup or buttermilk, raw or purified lactose, the resulting probiotic preparations containing 2 to 98% yeast derivatives or derivatives derived therefrom such as hydrolysates or derivatives derived therefrom such as hydrolysates or autolysates, the probiotic preparation for human nutrition may additionally be enriched with growth substances for bifidobacteria such as lactulose, growth lambs carrots and soybeans, or can be enriched with dry matter and lipidys of sea buckthorn berries.

The invention further relates to the aforementioned production, wherein the probiotic prodrugs are produced in a dried form without the addition of yeast biomass and their derivatives, with the exception of those components which can be used in the culture of bacterial cultures, including yeast extract with these prepaids. the fractions contain the following combinations of bacterial cultures, yoghurt culture, bulgaric lactobacillus or Streptococcus thermophilus. , optionally acrobacillus acidophilus, or Bifidobacteria and Streptococcus faecium, or yogurt culture, Lactobacus Husbulgaricus or Streptococcus thermophilus, Lactobacillus acidophilus / or bifidobacteria, lactobacillus acidophilus and Streptococcus phaecium, wherein the bifidobacteria culture itself is composed of km In addition to the symbiotic culture capable of growing under facultatively anaerobic or microaerophilic conditions, the resulting probiotic preparation contains 0 to 15% of yeast biomass, its hydrolyzate, autolysate and / or extract in the dry matter of the finished product, with the bacteria being cultured in a medium based on milk raw materials such as milk, pre-skimmed milk, whey, buttermilk, or prepared pastes thereof, raw or purified lakioza, starches and derivatives thereof may be added during manufacture, before or after fermentation, as well as modified starch and maltodextrins according to both methods The advantage of the process for the production of probiotics according to the invention is that the high addition of yeast biomass and its derivatives in concentrations of 2 to 5% by weight, preferably 0.5 to 5%. 98% hinot.se combines the effect of the yeast preparations themselves with the use micro-flora capable of passage or settlement of the digestive tract of humans and animals. In preparations where the yeast biomass is not added, the symbiosis of the types of bacteria or other growth substances according to the invention is used, whereby the preparation of tablets with the probiotic effect is prepared in the form of tablets or coating tablets, even from known combinations of cultures still in use. for recipes of fermented milk products or for semi-finished products intended for the manufacture of infant and baby food.

For human nutrition purposes, the probiotics of the invention may be further enriched or co-administered with vitamin preparations such as, in particular, vitamin C and vitamin A, or its precursors. In animal feeds, vitamin components are usually already contained. another advantage in that they are not a direct feed form but can be added to the food or can be given to the animals as a special nutritional additive, or acidified beverages can be prepared from these probiotic preparations in the preparation of feed for the animal. The present growth substances, which originate from the yeast bioassay, assist in the rapid restoration and growth of the microflora inoculated in the form of a probiotic preparation, for example, into whey or milk feed mixtures.

Dried seed cultures foamed in the prepared milk substrates, as described in the subject matter of the present invention, allow for tabletting even in the case of semi-finished products which have been used only for the purpose of infant and baby nutrition. biomaae, as well as varnishes and plant additives. Tablets can also be made in the form of dragees. The addition of maltodextrins in the nutrition tables for human nutrition is an energy source for the development of microflora present for consumption of probiotics *

Process for preparing probiotic preparation from yogurt culture or only from its individual species, ie Laotobacillus bul-garicus or Streptocoocus thermophilua, cultivated in milled milk after its previous treatment at 95 ° C, 30 minutes, cooling to 42-45 ° 0, wherein the milk solids can be adjusted to a range of 8.9 to 20%, after 2.5 to 3 hours of cultivation, the fermented milk is dried directly or after neutralization with 20% CaOH 2 or NaOH or NH 4 OB to pH 6 , 3 to 7.2. Beer »yeast, Saccharomyces laotis, Kluyveromyces fragilis or marxlanus for human nutrition and Candida utilis or Torulaspecies for animal nutrition are spray dried * A mixture containing eg 2 to yeast biomasses and 98 to 2% of dried yoghurt culture is prepared with a mixing device * For human nutrition purposes tablet mixture * 7 Example 2

A symbiotic culture containing Bifidobacteriumlongum and Bifidobacterium bifidum bifidobacteria, or even breve, infantis or adolescentis species, is prepared by culturing in a sterile hair dryer with 1 to 5% by weight of brewer's yeast, the pH of the substrate being adjusted to pH 2 to 3.5 with phosphoric acid and 45 ° C, partial enzymatic hydrolysis of proteins and yeast biomass is carried out with chicken or pork pepper. After 5 hours, the pH is adjusted to pH 6.8 to 7.5 with NaOH and the substrate is sterilized at 120 ° C for 20 minutes. Inofeulumbifidobacteria is used in an amount of 2 to 10% by volume and at 37 ° C, the substrate with a dry matter content of 6 to 15% by weight is used. zfermentujep for 16 hours, neutralized with NaOH to pH 6.8-7.2a and spray dried. The dried whey is mixed with the dried yeast yeast in such a ratio that the resulting dry matter content of the yeast biomass is 2 to 98%.

In order to make the drying process more efficient, the dry matter of the fermented whey can be adjusted to 25% by the addition of thickened whey. EXAMPLE 3 Example 2 is followed by using a symbiosis with Pediococcus acidilactici in a 1% vaccination dose for bifidobacterial culture. EXAMPLE 4 Example 2 and 3, with the use of propionbacterium shermanii and freuden reichii in fermentation. EXAMPLE 5 A culture in skimmed milk is prepared in the same manner as in the manufacture of infant formula by cooling the paste at 115 DEG C. in a tube paste with a 25-30 second holding temperature to a temperature of 30 DEG C. and 0.1% lac. -tobacillus acidophilus and 1% to 4% of Bifidobacterium longum, bifidum, breve, infantis and bifidobacteria cultures, respectively. ado-lescenntis. After 16 hours of cultivation, the fermented milk 8 is mixed with the concentrated skimmed or whole-fat milk with a dry weight of 40 to 50% by weight to the final dry weight of the semi-finished product 25 to 30% by weight and spray-dried, with the drying air outlet temperature of 68 to 80 ° C. the device of the vertical worm homogenizer type dried milky culture is mixed with dry yeast biomass originating in Saccharomyces cerevisia, carsbsbensis, Saccharomyces lactis, marxian or fragilis or autolysates and hydrolysates therefrom, so that the resulting yeast dry matter content of the product is 2 to 98% by weight Example 6

The process according to claim 5, wherein the cultivation is carried out in cheese or permeate from milk, whey or buttermilk and after fermentation, the resulting dry matter is mixed with the concentrated skim milk after the previous neutralization to a pH of 6.8 to 7.2. up to 30% by weight; another manufacturing process as in point 5 · Example 7

The method of manufacture as in 5 and by preparing the substrate for fermentation from buttermilk which is mixed with the fermented thickened milk after fermentation so that the mixture reaches a dry weight of 15 to 25% by weight prior to drying. corresponds to the description in section 5 · Example 8

The production process according to items 1 to 7, wherein the culture further comprises Streptococcus faecium in a vaccination dose of 1-2%. Example 9 In a skimmed or whole-fat high pasteurized milk 95 ° C30 minutes or 115 ° G 25-30 seconds, a symbiotic bi-fidogenic culture composed of bifidobacteria is cultivated with the working strains 3i-fidobacterium species strain 5 and strain 1 of Bifidobacterium bifidum, with strain 5 with its characteristics near Bifidobacterium lcngum. Cultivation is carried out at 37 ° C for 16 hours, and up to 30% by weight of yeast biomaey, in the dry matter of substrate, auto-lysate, hydrolyzate or extract of brewing yeast Saccharomycea oerevisiae and carlsbergensie, can be added to the dairy substrates prior to pastergy; the milk solids dry matter can be in the range of 8-7 to 20% · After the fermentation, the fermented medium is dried directly, previously neutralized with Ca (OH) 2 NaOH or NH 4 OH to pH 6.8 to 7.2 or dry-fermented the media is increased to 25 to 35% by the addition of concentrated centrifugal powder before spray drying ·

The dried culture thus prepared is mixed in a blender with dried brewing yeasts Saecharemyces oerevisiae aoarlsbengensis so that their ratio in the final product is 30 to 50% by weight of yeast dry matter, including that added during the cultivation. by not further enriching the dried preparation with yeast dry matter and serving for further use as a direct drying or tabletting process. That starch or its derivatives, such as heat-treated starch and raltodextrins, with dextro equivalent of 8 to 20, is added to the milk or other milk raw material prior to its heat treatment, in an amount such that the final product content is 0.5% by weight. For starch and 5% to 10% by weight of maltodoxtriums. A further manufacturing process is the same as in 1 to 10. Example 12. Example 1 to 9 with bacterial cultures containing Lactobacillus casei in a 1% vaccination dose. EXAMPLE 13 Example 1 to 11, wherein the working culture for the preparation of the pre-drying preparation contains Lactobacillus acidophilus in a 0.1 to 0.5% seed dose. EXAMPLE 14 Example 9 with the use of Lactobacillus acidophilus in the preparation for drying culture. 0.1% 10% Pediococcus acidilactici in a 1% vaccination dose. Example 15

In high pasteurized skim milk of 115 ° C with a 25 sec duration, the culture of Lactobacillus acidophilus, inoculum 0.1% and Bifidobacterium longum and bifidobacterium bifidum, cultures 4: 1, bifidobacterial inoculation dose 2-4%, skim milk solids in culture 15 to 20%, the temperature of the cultivation is 37 ° C, the time of 16 hours, the culture medium contains 6.5% of yeast biomass, hydrolyzate, or extract in the case of dairy substrate starting solids of 15% and 4.9% of yeast biomass or their derivatives in case of of dairy substrate with a dry matter content of 20%. Prior to drying, the fermented substrate is mixed with thickened milk having a dry weight of 40% and the resulting dry mix is adjusted to 25%, the dried preparation then contains 15% yeast biomass or derived derivatives thereof. Example 16

The fermentation medium is milk permeate, whey or buttermilk. Its dry matter is adjusted to 8% by evaporation or the permeate is used directly with a starting dry weight of 4.5 to 5%, the pH is adjusted to pH 6.8 to 7.2 by 20% NaOH solution, and after 95 ° C pasteurization for 30 minutes, the substrate is inoculated with the cultures mentioned in point 2 of the description of the invention, for example with Lactobacillus acidophilus Streptococcus faecium or Bifidobacteria and Streptococcus faecium cultures such that the inoculum dose of acidophilic culture corresponds to 0.2 to 1% and Streptococcus faecium 1% and bifidobacteria respectively with the deml5. The cultivation takes place at 37 ° C for 14-18 hours.

The fermented medium is then concentrated by drying with 35% dry whey to a final dry weight of at least 20% prior to further drying. Prior to tabletting, this preparation can be mixed with dried yeast yeast in a 1: 1 ratio. Without the addition of yeast, jetabletting is facilitated by the addition of 50% skimmed milk powder. EXAMPLE 17 Example 1 to 16, wherein the fermented milk substrates are not dried for animal feed, if the fermented biomass is added, or the derivatives derived therefrom are in the range of 2 to 20% by weight of the mixture after fermentation or after mixing the fermented medium with the yeast semifinished products. Yeast semifinished products can be added in both dry and liquid form. EXAMPLE 18 Example 1 to 17, with the proviso that the base of the fermentation medium consists of crude or purified lactose at a concentration of 5 to 7% by weight. Prior to drying, the fermented medium can be concentrated by ultrafiltration to dry matter 20%, in order to favor the economy of drying, to reduce the loss of live bacteria and to achieve physical properties of the semi-finished product, in particular before its tabletting, the fermented medium can be mixed with thickened skimmed milk with a dry weight of 35-50%, with the resultant semi-finished dry matter being 20-30% and followed by spray drying.

The blank may be used for feed purposes in this form, for use in human nutrition after tabletting, preferably as a tablet.

Claims (2)

A method for the production of probiotic preparations for human and animal nutrition, characterized in that probiotic preparations in dried form, preferably in the form of tablets and for the preparation of human beings, are produced for human nutrition. In addition, live animals can also be prepared in liquid form, containing yeast biomass or hydrolysates or autolysates prepared therefrom, preferably Saccharomyces ceevisiae yeast, Saccharomyces carlsbengensis, Kluyverorayces lactis for human nutrition. , Saccharomyces fragilis and Kluyveromyces raaratianus, in addition to yeasts used in the feed of genusCandida and Torula together with bacteria capable of passage or settlement of the digestive tract of humans and animals such as Lac-tobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, preferably mucilaginous strain, Streptococcus sali variussubsp * thermophilus, preferably a mucilaginous strain, Streptococcusphaecium, Bifidobacteria with the exception of Bifidobacterium dentium, preferably Bifidobacterium longum, bifidum, breve, infantias or adolescentis, and in addition may contain probiotics as additional bacteria of the genus Propionibacterium such as Propionibacterium shermanii and freudenreichii or Pediococcusacidilactici, bacterial cultures are prepared by culturing in a milk-based medium, preferably skimmed milk, whey, buttermilk, milk permeate, whey or buttermilk, raw or purified lactose, the resulting pro-biotic preparations containing 2 to 98% of the biomass biomass or derivatives derived therefrom, such as hydrolysates or autolysates, a probiotic preparation for human nutrition may additionally be enriched with growth substances for Bifidobacteria such as lactulose, plant-derived growth substances contained in the dry matter carrots and soybeans, or they can be enriched with dry matter and lipids from sea buckthorn seeds · 2. The process according to claim 1, wherein the probiotic preparations are produced in dried form without addition of fermented biomass and their derivatives, with the exception of those which can be used in the cultivation of bacterial cultures including yeast extract, the probiotic preparations containing the following combinations of bacterial cultures. bifidobacteria except Bifidobacterium dentium, and Pediococcus acldilactici, yoghurt culture, Lactobacillus bulgaricus or Streptococcus thermophilus and Streptococcus faecium, optionally Lactobacillus acidophilus, or Bifidobacteria and Strepococcus faecium , or yoghurt cultures, Lactobacillus bulgaricus or Streptococcus thermophilus, ob actobacillus acidophilus / or Bifidobacteria, Lactobacillus acidophilus and Streptococcusphaecium, wherein the bifidobacteria culture itself is a complex of strains in a symbiotic culture capable of growing under facultative anaerobic and microaerophilic conditions, the resulting probiotic preparation contains 0 to 15% yeast biomass, its hydrolyzate, autolysate and / or extract in the dry product of the finished product, the bacteria being cultured in a medium based on milk raw materials such as milk, preferably skimmed milk, whey, buttermilk, or permeate prepared therefrom, raw or purified lactose, during manufacture, before or after fermentation, may be added with starches and their derivatives, such as heat-treated starch and maltodextrins, according to both the production methods contained in 1 and 2, the content of which in the final product may be up to 10%, preferably 0.5 to 5%.