CO2023018191A2 - Métodos de purificación de columnas de vectores de aav - Google Patents
Métodos de purificación de columnas de vectores de aavInfo
- Publication number
- CO2023018191A2 CO2023018191A2 CONC2023/0018191A CO2023018191A CO2023018191A2 CO 2023018191 A2 CO2023018191 A2 CO 2023018191A2 CO 2023018191 A CO2023018191 A CO 2023018191A CO 2023018191 A2 CO2023018191 A2 CO 2023018191A2
- Authority
- CO
- Colombia
- Prior art keywords
- purification methods
- aav vector
- column purification
- vector column
- particles
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163209680P | 2021-06-11 | 2021-06-11 | |
| US202263366094P | 2022-06-09 | 2022-06-09 | |
| PCT/US2022/072859 WO2022261663A1 (en) | 2021-06-11 | 2022-06-10 | Aav vector column purification methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO2023018191A2 true CO2023018191A2 (es) | 2024-01-25 |
Family
ID=84426294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CONC2023/0018191A CO2023018191A2 (es) | 2021-06-11 | 2023-12-21 | Métodos de purificación de columnas de vectores de aav |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20240271159A1 (enExample) |
| EP (1) | EP4351755A4 (enExample) |
| JP (1) | JP2024525142A (enExample) |
| KR (1) | KR20240021231A (enExample) |
| AU (1) | AU2022289035A1 (enExample) |
| BR (1) | BR112023025999A2 (enExample) |
| CA (1) | CA3221540A1 (enExample) |
| CL (1) | CL2023003646A1 (enExample) |
| CO (1) | CO2023018191A2 (enExample) |
| IL (1) | IL309070A (enExample) |
| MX (1) | MX2023014809A (enExample) |
| PE (1) | PE20240359A1 (enExample) |
| WO (1) | WO2022261663A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2025528182A (ja) * | 2022-08-12 | 2025-08-26 | ウルトラジェニックス ファーマシューティカル インコーポレイテッド | 完全な組換えアデノ関連性ウイルス粒子から空の粒子を分離するための新規な陰イオン交換クロマトグラフィーの方法 |
| EP4670812A1 (en) * | 2024-06-27 | 2025-12-31 | Sartorius Stedim Biotech GmbH | CONTINUOUS POLISHING OF VIRAL CAPSIDES |
| WO2026064763A1 (en) * | 2024-09-23 | 2026-03-26 | Regeneron Pharmaceuticals, Inc. | Partitioning anion exchange chromatography for purification of recombinant adeno-associated virus (raav) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7261544B2 (en) * | 2003-05-21 | 2007-08-28 | Genzyme Corporation | Methods for producing preparations of recombinant AAV virions substantially free of empty capsids |
| US20120093775A1 (en) * | 2009-03-27 | 2012-04-19 | Proyecto De Biomedicina Cima, S.L. | Methods and compositions for the treatment of cirrhosis and liver fibrosis |
| PE20200737A1 (es) * | 2017-06-30 | 2020-07-23 | Spark Therapeutics Inc | Metodos de purificacion de columna de vector aav |
| WO2023077086A1 (en) * | 2021-10-29 | 2023-05-04 | Homology Medicines, Inc. | Methods and compositions for the purification of adeno-associated virus |
| CN120283059A (zh) * | 2022-10-11 | 2025-07-08 | 梅里特斯英国第二有限公司 | 纯化aav衣壳的色谱方法 |
-
2022
- 2022-06-10 WO PCT/US2022/072859 patent/WO2022261663A1/en not_active Ceased
- 2022-06-10 MX MX2023014809A patent/MX2023014809A/es unknown
- 2022-06-10 JP JP2023576326A patent/JP2024525142A/ja active Pending
- 2022-06-10 US US18/568,530 patent/US20240271159A1/en active Pending
- 2022-06-10 AU AU2022289035A patent/AU2022289035A1/en active Pending
- 2022-06-10 PE PE2023003271A patent/PE20240359A1/es unknown
- 2022-06-10 CA CA3221540A patent/CA3221540A1/en active Pending
- 2022-06-10 BR BR112023025999A patent/BR112023025999A2/pt unknown
- 2022-06-10 IL IL309070A patent/IL309070A/en unknown
- 2022-06-10 EP EP22821261.9A patent/EP4351755A4/en active Pending
- 2022-06-10 KR KR1020247000822A patent/KR20240021231A/ko active Pending
-
2023
- 2023-12-05 CL CL2023003646A patent/CL2023003646A1/es unknown
- 2023-12-21 CO CONC2023/0018191A patent/CO2023018191A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2022289035A1 (en) | 2024-01-04 |
| EP4351755A1 (en) | 2024-04-17 |
| CA3221540A1 (en) | 2022-12-15 |
| EP4351755A4 (en) | 2025-04-23 |
| JP2024525142A (ja) | 2024-07-10 |
| KR20240021231A (ko) | 2024-02-16 |
| US20240271159A1 (en) | 2024-08-15 |
| BR112023025999A2 (pt) | 2024-02-27 |
| IL309070A (en) | 2024-02-01 |
| PE20240359A1 (es) | 2024-02-27 |
| WO2022261663A1 (en) | 2022-12-15 |
| CL2023003646A1 (es) | 2024-10-25 |
| MX2023014809A (es) | 2024-01-29 |
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