CO2019014075A2 - Métodos para la determinación del género de embriones aviares en huevos sin eclosionar y medios de los mismos - Google Patents

Métodos para la determinación del género de embriones aviares en huevos sin eclosionar y medios de los mismos

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Publication number
CO2019014075A2
CO2019014075A2 CONC2019/0014075A CO2019014075A CO2019014075A2 CO 2019014075 A2 CO2019014075 A2 CO 2019014075A2 CO 2019014075 A CO2019014075 A CO 2019014075A CO 2019014075 A2 CO2019014075 A2 CO 2019014075A2
Authority
CO
Colombia
Prior art keywords
gender
determination
methods
avian
avian embryos
Prior art date
Application number
CONC2019/0014075A
Other languages
English (en)
Inventor
Daniel Offen
Original Assignee
Eggxyt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=62716101&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CO2019014075(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eggxyt Ltd filed Critical Eggxyt Ltd
Publication of CO2019014075A2 publication Critical patent/CO2019014075A2/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/072Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/30Bird
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/01Animal expressing industrially exogenous proteins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • A01K2267/0393Animal model comprising a reporter system for screening tests
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a métodos de fertilización y determinación e identificación de género en sujetos aviares. Más específicamente, la invención proporciona métodos no invasivos que usan animales aviares transgénicos que comprenden al menos un gen indicador, específicamente, RFP, integrado en al menos un cromosoma de género Z o W. de género. Los animales aviares transgénicos de la invención se usan para la determinación de género y selección de embriones en huevos aviarios sin eclosionar.
CONC2019/0014075A 2017-05-25 2019-12-13 Métodos para la determinación del género de embriones aviares en huevos sin eclosionar y medios de los mismos CO2019014075A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762510921P 2017-05-25 2017-05-25
PCT/IL2018/050573 WO2018216022A1 (en) 2017-05-25 2018-05-24 Methods for gender determination of avian embryos in unhatched eggs and means thereof

Publications (1)

Publication Number Publication Date
CO2019014075A2 true CO2019014075A2 (es) 2020-04-01

Family

ID=62716101

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2019/0014075A CO2019014075A2 (es) 2017-05-25 2019-12-13 Métodos para la determinación del género de embriones aviares en huevos sin eclosionar y medios de los mismos

Country Status (23)

Country Link
US (1) US20200283849A1 (es)
EP (1) EP3629719B1 (es)
JP (1) JP7179776B2 (es)
KR (1) KR20200018486A (es)
CN (2) CN114836475A (es)
AU (1) AU2018274769B2 (es)
BR (1) BR112019024642A2 (es)
CA (1) CA3064825A1 (es)
CL (1) CL2019003414A1 (es)
CO (1) CO2019014075A2 (es)
CR (1) CR20190569A (es)
CU (1) CU20190094A7 (es)
DO (1) DOP2019000298A (es)
EA (1) EA201992582A1 (es)
EC (1) ECSP19087406A (es)
IL (1) IL270903B1 (es)
MA (1) MA48783A (es)
NI (1) NI201900121A (es)
PE (1) PE20200692A1 (es)
PH (1) PH12019502663A1 (es)
PL (1) PL3629719T3 (es)
SG (1) SG11201911686YA (es)
WO (1) WO2018216022A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112522315B (zh) * 2020-12-08 2023-04-11 广东省农业科学院动物科学研究所 一种鸡原始生殖细胞转染方法
CN112956427B (zh) * 2021-01-29 2022-06-24 四川省畜牧科学研究院 一种便捷式雏禽性别鉴定肛笔
CN114568343B (zh) * 2022-03-11 2023-04-07 成都合拓创展生物科技有限公司 制备眼病模型的头具及其应用
CN114793988B (zh) * 2022-04-19 2023-01-17 中国科学院海洋研究所 一种基于形态学鉴定孔鳐受精卵的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9511439D0 (en) 1995-06-06 1995-08-02 Isis Innovation Gene product and method
AUPO060996A0 (en) 1996-06-21 1996-07-18 University Of Melbourne, The Sexing gene
CA2264450A1 (en) 1998-03-05 1999-09-05 Andras Nagy Methods for monitoring heterologous sex chromosomes
US6244214B1 (en) 1999-01-06 2001-06-12 Embrex, Inc. Concurrent in ovo injection and detection method and apparatus
CN1761756B (zh) * 2003-01-16 2013-03-13 北卡罗来纳州大学 禽类物种中内源性原生殖细胞的耗减
CN101218506A (zh) 2005-05-11 2008-07-09 恩布里克斯公司 利用荧光显微镜的竞争性粒子免疫测定法
CN102421282B (zh) * 2009-03-13 2015-06-10 范德冯管理有限公司 鸟类性别判定的方法
FR2969497B1 (fr) 2010-12-27 2013-06-28 Ceva Sante Animale Composition luminescente comme biomarqueur dans un oeuf aviaire, dispositif et procede correspondants.
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
DE202013011765U1 (de) 2012-08-31 2014-05-26 Mat-Malta Advanced Technologies Limited Spektrophotometrische Analyse der Federfarbe von Hühnerembryos
PT3080279T (pt) * 2013-12-11 2018-12-17 Regeneron Pharma Métodos e composições para a modificação direcionada de um genoma
FR3023617B1 (fr) 2014-07-11 2018-06-15 Tronico Dispositif non invasif de determination de la fertilite et/ou du sexe d’un oeuf, et procede correspondant.
JP2019505175A (ja) 2015-12-03 2019-02-28 エッグクシト,リミテッド 鳥の未孵化卵内の胚の性別決定法及びその手段

Also Published As

Publication number Publication date
PL3629719T3 (pl) 2023-11-27
CR20190569A (es) 2020-04-19
MA48783A (fr) 2020-04-08
EP3629719C0 (en) 2023-08-23
ECSP19087406A (es) 2020-01-31
JP7179776B2 (ja) 2022-11-29
CA3064825A1 (en) 2018-11-29
AU2018274769A1 (en) 2019-12-19
AU2018274769B2 (en) 2024-05-02
PH12019502663A1 (en) 2020-09-28
IL270903B1 (en) 2024-03-01
EP3629719A1 (en) 2020-04-08
KR20200018486A (ko) 2020-02-19
WO2018216022A1 (en) 2018-11-29
EP3629719B1 (en) 2023-08-23
CN110891418A (zh) 2020-03-17
DOP2019000298A (es) 2020-09-30
EA201992582A1 (ru) 2020-05-19
CL2019003414A1 (es) 2020-08-07
BR112019024642A2 (pt) 2020-06-23
NI201900121A (es) 2020-02-14
CU20190094A7 (es) 2020-10-20
SG11201911686YA (en) 2020-01-30
US20200283849A1 (en) 2020-09-10
CN110891418B (zh) 2022-05-27
PE20200692A1 (es) 2020-06-11
CN114836475A (zh) 2022-08-02
JP2020520669A (ja) 2020-07-16
IL270903A (en) 2020-01-30

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