CN87100676A - The method of growth and development of plants and device - Google Patents

Abstract

One plant development system and in cellular cultivation matrix, leave with in micropropagation vegetable material and each culturing room from this matrix and to transplant plantlet to corresponding culture device, in the breeding dish.The cellular cultivation matrix that is used to cultivate the plant tissue brood body is also described, and proposes protection.

Description

The method of growth and development of plants and device

The invention relates to a plant development device, especially about on cellular cultivation matrix, leaving with the micropropagation vegetable material and plantlet being transplanted in the cultivation equipment, in the breeding dish respectively from each culturing room of this cultivation matrix.The present invention is also about a kind of cellular cultivation matrix of cultivating the plant tissue brood body.

On March 13rd, 1985, disclosed No. 0132414 european patent application was described the method for cultivating the micropropagation vegetable material.This method is that vegetable material is placed in the screen cloth of medium top.Adopt the uniformity of the method organization of production culture in above-mentioned disclosed No. 0132414 european patent application and the distributing homogeneity that quality depends on media surface top brood body in the culture vessel.

The conventional method of cultivating the plant tissue brood body is widely used, and many comment report documents are also arranged.For example, in " in vitro breeding " (Avery, 1982) of P.F.Wetherall; B.V.Conger, Ed; In T.A.Thorpe " method for plant tissue culture and the application on agricultural " (science version, 1981), all the current practice of this respect has been done introduction.In brief, in vitro breeding is exactly under aseptic condition, as the lower part plant corpus, and puts it in the nutrient media that can impel its growth and/or propagation.More particularly, plant tissue culture course is divided into three orderly Main Stage.The task of phase I is to allow the aseptic culture thing begin rapid growth.This culture can bring out from some plant organs.

Generally speaking, explant after surface sterilization, is handled under aseptic condition again.After the sterilization, with sterile distilled water several times, be cut into satisfactory fragment with the apparatus of sterilizing again, put it into then in the adjustable culturing room of light quantity, photoperiod and temperature the explant fine laundering.

In culturing room through about 6-8 after week, the breeding that begins in vitro to breed with the explant of growth.At this moment the explant of growing to be shifted out from culture vessel under aseptic condition, can repair and cut in case of necessity, then, put into the culture vessel of the medium that contains suitable hormone component again, carry out equilibrium and bring out propagation.Again this culture vessel is put back in the culturing room.These plant tissues shift out culture vessel under aseptic condition after spending the time in about 6-8 week once again on culturing room's internal breeding medium, transfer to after the cutting on the fresh medium, send culturing room again back to.The current transfer, both having can be transferred to bringing out has root or does not have tallage and grow on the growth medium of preparing, and makes the transition that can stand after the young shoot to greenhouse, also can transfer on the proliferated culture medium further propagation once more.

The plant tissue cultures repetitive cycling causes owing to breeding rapidly through the ability of multiplicative stage (stage II).Breeding is one of major advantage of in vitro breeding rapidly.But, labour intensity is very big in this process, it is estimated, the money that is used for salary and auxiliary cost accounts for the 60-65% of the used cost that in vitro cultivates plants, (" adopting the cost of Plant Tissue Breeding breeding broccoli " of W.C.Anderson G.W.Meagher and A.G.Nelson, Hertscience 12:543-544,1972); (A.Donnnan, Jr.S.E.Davidson and C.L.Williams, " in greenhouse plantation through the plant of Plant Tissue Breeding " Proc, Fla.State.Hort.Soc 91:235-237,1978).Therefore, people are carrying out various trials, reduce the expense relevant with manual labor.

A kind of trial that above-mentioned disclosed No. 0132414 european patent application is done has obtained success.This paper is with reference to this point.But, the applicant finds that with a kind of indiscriminate screen cloth carrying vegetable material, not being very suitable for therefrom, machinery shifts out plantlet on medium.

The present invention tries hard to be provided for plantlet growth, growth and implantation technique and mechanization system.

According to a kind of embodiment according to qualifications of the present invention, the growth and development of plants system that includes cellular cultivation matrix of phase I that is provided, this matrix comprises the support bottom surface of liquid permeable and supports the parietal layer structure of arranging independent culturing room on the bottom surface by first of dress that little culturing room contiguous among the little culturing room that these are independent and first row is separated by first spacing; Constitute the cultivation supporter of the second stage of the independent plantlet vitellarium of second row, contiguous little vitellarium is separated by second spacing among the little vitellarium that these are independent and second row, and second spacing is greater than first spacing; Propagulum roughly is evenly distributed to the device on the cellular cultivation matrix of the phase I of containing medium; Utilize that mechanical means will little culturing room moves on to the device of each corresponding vitellarium on the cultivation supporter of second stage from each at plantlet that first row cultivate in the little culturing room.

According to a kind of embodiment according to qualifications of the present invention, the method for following growth and development of plants also is provided, step comprises:

The cellular cultivation matrix of phase I is set, this matrix comprises the support bottom surface of liquid permeable and the parietal layer structure of the independent little culturing room of a row is set that adjacent little culturing room is separated by first spacing among the little culturing room that these are independent and first row above the support bottom surface;

The cultivation supporter of second stage is set, is made of the independent plantlet vitellarium of second row, little vitellarium adjacent among the plantlet vitellarium that these are independent and second row is separated by second spacing greater than first spacing;

Propagulum is evenly distributed on the cellular cultivation matrix of the phase I that medium is housed;

The plantlet that utilizes mechanical means that first row is cultivated in the little culturing room moves on to each corresponding vitellarium on the cultivation supporter of second stage from each little culturing room.

According to a kind of embodiment according to qualifications of the present invention, each little culturing room all is inverted frustum, and machinery shifts out the plantlet of the inside to help automatically.

In addition, according to a kind of embodiment according to qualifications of the present invention, being used for machinery shifts out, the device of transplanting comprises: be used for that plantlet first row from the cellular cultivation matrix of phase I is on the cultivation supporter of being transplanted to second stage the little culturing room that the regular grids shape arranges second row and be the regular grids shape and arrange, and each center distance is greater than the device in each OC each vitellarium of the little culturing room of first row, and this device comprises:

The carrier of a plurality of probes is housed, and these probes are by the arrangement as regular grid, and its spacing center distance with the second row vitellarium basically is the same;

Cause the device of the relative displacement between the carrier and first and second rows, probe is aimed at first row's little culturing room earlier, and then aim at each vitellarium of second row respectively by this displacement;

Be used for extracting the plantlet in each little culturing room among first row, and hold it on each probe, when probe is aimed at second each vitellarium of row respectively, at last plantlet is put into the device of the corresponding vitellarium of second row.

According to a kind of embodiment of the present invention, cause the device of relative displacement to comprise: the device that moves carrier along first, second and third vertical axial.

According to another embodiment of the present invention, cause the device of relative displacement to comprise: to move the device of carrier and make the first, two row along the first, two vertical axial at least along the 3rd device of changing perpendicular to the first, two.

The multistage growth and development of plants system that the present invention provides simultaneously comprises the cellular cultivation matrix of phase I, this matrix comprise the screen cloth bottom surface and above screen cloth, be provided with one row little culturing room the upright parietal layer structure that is grid-like arrangement; Propagulum is evenly distributed on the cellular cultivation matrix of phase I, and the device that the plantlet of requirement is grown on this cultivation matrix; And will take out automatically in the culturing room of plantlet from the cellular cultivation matrix of phase I, and put it into device in the culturing room in the culture plate of the size second stage big than cellular cultivation matrix of phase I.

In addition, each step that the technology of the multistage plant growth and development process that a kind of embodiment according to qualifications according to the present invention provides comprises is: the cellular cultivation matrix that the phase I is set, this matrix comprises the screen cloth bottom surface and constitute the upright parietal layer structure that is grid-like arrangement of a row culturing room on screen cloth, propagulum is evenly distributed on the cellular cultivation matrix of phase I, basically make plantlet development growth on the cellular cultivation matrix of phase I of requirement, and automatically by taking out plantlet in the culturing room on the cellular cultivation matrix of phase I, and put it into size and cultivate greater than the phase I in the culturing room on the culture plate of second stage of matrix.

In addition, according to a kind of embodiment according to qualifications of the present invention, provide a kind of propagulum is evenly distributed on to be installed in contain, method on the cellular cultivation matrix of phase I in the container of lower chambers, each step that this method comprises is: the liquid mixture that will contain the Plant Tissue Breeding brood body is packed in the upper chamber in the container of bottom sealing, feed bubble stream from air intake, bubble is upward through the most of effective cross section of container, fully stir, brood body in the mixing material, make the uniform basically suspension of formation, allow liquid discharge, the brood body of suspension is evenly distributed on this matrix basically by cultivating matrix.

The present invention also provides and has been used for propagulum is evenly distributed on device on the cultivation matrix of phase I, this matrix is contained in sealable, be divided in the container of upper and lower chamber, this container comprises that input contains the controlled defeated material mouth of the liquid mixture of Plant Tissue Breeding brood body, controlled discharging opening, and the controlled air intake that joins with the bubble distributor.

The invention provides a kind of sterilizable, the container that not only can open but also can close and seal, this container is equipped with movably, sterilizable, basically be flat and fine and closely woven cultivation matrix, this cultivation matrix can completely be divided into upper and lower two parts with this container, container top is equipped with and is the charging aperture of sending into propagulum/liquid mixture and inlet valve, the air venting valve preferably also is housed simultaneously, converted steel distributor pedestal is housed below the screen cloth of container lower part, is discharging opening and intake valve below the distributor.

In one the of the present invention embodiment of selecting the superior, the approximate square of container shapes, the about 8cm of the inside dimension on limit is made up of discerptible two chambers, and upper chamber accounts for 2/3rds of container volume, can rise and fall under the air rammer effect, and lower chambers is fixed.Cultivate matrix and be fixed on the framework, the seamed edge of framework is equipped with, and extends the edge of lower chambers, when closure leaves standstill under the effect of air rammer pressure with Bedpan, is utilized the water seal sealing by the screen cloth between the upper and lower chamber of container.

It is all the most handy sterilizable to cultivate matrix and framework, and very the material of inertia is made, and as making container with stainless steel, makes screen cloth and framework with nylon.

As mentioned above, the present invention is mainly used in by automation process from the production of tissue culture plantlet.Plant tissue must move on in the culture vessel by mechanical means after aseptic cutting and/or dividing heap, and under aseptic condition, on the multiple medium in container in shop, impels plant tissue growth to grow in this way.This process comprises that also the plant tissue that will determine amount floats on a liquid, and installs to the suspension branch in the device that below will also describe by certain volume.This device is positioned at the culture vessel top that solid or liquid nutrient medium are housed, plant tissue can be evenly distributed on and cultivate on the matrix, so that being deposited at last, cultivates on the matrix vegetable material, and fully contact with it, as described in disclosed No. 0132414 european patent application.

Another advantage of the present invention is to have saved the storage narrow-necked earthen jar that the disclosed No. 0132414 described automation micropropagation of european patent application process is used.This article disclosed method is by the brood body/liquid suspension in the aseptic storage narrow-necked earthen jar of continuous stirring, and propagulum is evenly distributed in liquid.Because the present invention adopts distributor chamber to reach even distribution, therefore, in automation micropropagation process, no longer needs with the described this storage narrow-necked earthen jar of above-mentioned No. 0132414 european patent application.

In addition, according to a kind of embodiment according to qualifications of the present invention, being used to of providing, the cultivate plants medium body structure of tissue culture propagating body comprised the little culturing room of the lattice-shaped tight spacing of row's specification, the upper and lower perforation of little culturing room, and the position that is in evenly, fixes between its size plantlet of wanting to keep growing each little culturing room in.

Therefore, in the embodiment of selecting the superior, the invention provides a kind of plug-in unit that is used for plant tissue culture vessel, this culture vessel is made of the cultivation matrix that a little culturing room of arranging the lattice-shaped tight spacing of rule forms, in the little culturing room, the following perforation, general 7mm height, 7mm is wide, and the shape of each little culturing room seems down truncated pyramid most, that is: the end face opening is basically greater than the bottom surface opening, inwall is the inclined plane, and plug-in unit is sterilizable, is contained in the culture vessel, be in same horizontal plane with the container top of filling medium, and fully contact with medium.

Best, frusto-pyramidal, the about 12mm of the about 5-of width are inverted in each little culturing room.After the medium body structure is processed separately, refill on the screen surface of culture vessel, perhaps add and be about to its bottom surface man-hour and link in the flat fine and closely woven screening and supporting member, the mesh of this member can make medium pass through, and simultaneously the tissue culture propagating body is stayed in its surface.

Little culturing room structure preferably selects for use sterilizable material such as nylon to make, and constitutes an integral body with the screening of plastics and supporting member.

A kind of embodiment of the present invention provides the plant tissue culture vessel that can carry out disinfection.It is characterized in that above this container interior bottom surface, having a flat basically fine and closely woven screening and support substrate member frame, this container is sealable basically, be applicable to cultivate and be deposited on above-mentioned screening and supporting member surface, the plant tissue cultures that fully contacts with medium, this medium is loaded in container and above-mentioned screening and the fair place of supporting member, the size that screening and supporting member are had is wanted above-mentioned medium is passed through, simultaneously above-mentioned tissue culture is kept in its surface, this component surface also is equipped with the little culturing room structure for the tissue culture propagating body and function that cultivates plants, this little culturing room structure comprises the lattice-shaped tight spacing of a row rule, the little culturing room of up/down perforation, the size of little culturing room is wanted to remain on and is in evenly fixing position between the plantlet that wherein grows basically.

One embodiment of the invention relate to the regular lattice-shaped of a row, the cultivation matrix of up/down perforation type culturing room, its shape resembles inverted truncated pyramid, aseptic plant tissue can be put into wherein, it is trapped on the medium, and fully contact with medium, make the vegetable material in each little culturing room be grown to one or cluster plantlet with this, each plantlet entity is independent of all other plantlets on the fixed position in the culture vessel.

This culture vessel base insert has many purposes in the Plant Tissue Breeding reproductive process, and is specially adapted to the whole bag of tricks of plant automatic micropropagation process.For example, it helps described here, and the operation running of the micropropagation transplantation device relevant with Figure 11-19 is because grafting device can only shift out the plantlet that is separated from each other, so should account for definite position in culture vessel.In addition, described the sort of when being semi-automatic method for plant tissue culture logotype at least with disclosed No. 0132414 european patent application, the also available such machine relevant with Figure 11-19 described here transplanted the plantlet of cultivating with this method, can provide so again to be applicable to greenhouse and breadboard automation micropropagation system.

Another advantage of cultivating matrix is to help plantlet is shifted out the shipment shipping from culture vessel.

Cultivating matrix and also have an advantage, is when adopting the agar overcoat, if for the rhizome that is wrapped on the screen cloth is separated to accelerate transplanting with plantlet, in the time of taking down screen cloth, the taper parietal layer of little culturing room can make fixedly unlikely the coming off of agar plug of plantlet.

According to a kind of embodiment according to qualifications of the present invention, also provide and be used for connecting the device that culture in the little culturing room moves on to each vitellarium in second row's regular grids cultivating matrix first row's regular grids shape, dot spacing wants big in each vitellarium mid point gap ratio first each little culturing room of row of second row, basic rate equals one times, this device comprises the carrier that one group of probe is installed, the arrangement of these probes is the grid-like arrangement of rule, and the spacing distance of each syringe needle spacing with second each central point of row vitellarium basically is the same; Cause the device of relative displacement between carrier and first and second row, by this displacement, probe can be aimed at the little culturing room of first row earlier, and then aim at second row in each vitellarium, be used for extracting culture in first each little culturing room of row, it is retained in each probe corresponding vitellarium in these probe alignment second row, puts it into the device of corresponding vitellarium among second row at last.

This device can shift out plantlet simultaneously by a plurality of probes of arranging with a kind of fixed form in the culturing room of a plurality of phase I cultivation matrixes, and it is moved into its length and width all greater than in row's regular grids shape vitellarium of cultivating little culturing room interval on the matrix.

On the other hand, the invention provides with tubular probe open top, culture in the little culturing room of bottom of which has holes is moved into the device of second vitellarium by first vitellarium, this device comprises the member that carries out relative displacement continuously between the device that causes probe and formation first and second vitellariums, by this displacement, probe is aimed at the opening of the little culturing room in first vitellarium earlier, aim at second vitellarium again; Compressed air or other compressed fluid pressure injection device in the culturing room is provided, when the opening of the medium and small culturing room in probe alignment first vitellarium, the culture in the culturing room can be sprayed in the probe; And the another set of pressure injection device that compressed air or other compressed fluid are provided, when probe alignment second vitellarium, culture can be sprayed in second vitellarium.

Tubular probe bottom or openend have a frusta-pyramidal chamber down, its tapering up, the culture in the culturing room of perpendicular alignmnet probe top, be ejected into by institute's applied pressure below the little culturing room indoor, and by the wedge jail.A ventilation hole will be arranged at least at the top of this chamber, when moving on the little culturing room culture, air can be discharged from this hole.

Tubular probe can be with a push rod or pogo stick, and after push rod retracted to above position, upper end, awl chamber, butt garden, it is indoor that culture upwards sprays into taper again in the little culturing room.When probe alignment second vitellarium or graft area, push rod moves down, and by the above-mentioned pressure injection that is applied on the probe, little culturing room culture is sprayed in the graft area from the frustoconical chamber.Push rod can be by moving on to advanced position on the back-moving spring.

Can below little culturing room, apply in the above-mentioned pressure current, apply suction pressure to probe, like this, both can when not having back-moving spring, make the push rod retraction, again can be when being unkitted push rod, it is indoor to assist in the little culturing room culture upwards to move into frustum, and is trapped in that this is indoor.

Carry out detailed description below in conjunction with accompanying drawing, you will be understood fully to the present invention.

Fig. 1 is a front view of being opened back internal unit structure according to one embodiment of the invention at the shell of tank that is adopted.

Fig. 2 is the partial view that equipment bottom container shown in Figure 1 is amplified.

Fig. 3 is the partial view that its upper side container shown in Figure 1 amplifies.

Fig. 4 is an equipment shown in Figure 1, along IV-represented sectioned top view of IV cutting plane.

Fig. 5 is the top view of phase I growing substrate and its support.

Fig. 6 is that container shown in Figure 1 is under closed state, along office's end view that VI-VI cutting plane partly dissects.

Fig. 7 is the top view of the culturing room in the phase I growing substrate according to the present invention.

Fig. 8 is a culturing room shown in Figure 7, along II-II cutting plane part view profile.

Fig. 9 is the local section elevation that the tissue culture vessel of phase I cultivation matrix is housed according to of the present invention.

To be tissue culture vessel taking lid and cultivate the top view of matrix in the phase I at this place away to Figure 10.

Figure 11 is the front view according to implanted device of the present invention.

Figure 12 is the top view after II-II direction biopsy cavity marker devices shown in Figure 11.

Figure 13 is a transplanting dish shown in Figure 12, and the chart of overlapping is in the above indicated, and only is to be used for illustrating cellular phase I cultivation matrix.

Figure 14 is according to an embodiment provided by the invention, supports the vertical sectional view of cellular phase I cultivation matrix probe carrier and a plurality of probe bed boards.

Figure 15 A and Figure 15 B are respectively front view and the end views according to another embodiment of the invention implanted device.

Figure 16 is the top view of the II shown in Figure 11 A-II hatching partial cut.

Figure 17 is according to the vertical sectional view that supports cellular phase I cultivation matrix probe carrier and a plurality of probe bed boards in the embodiment provided by the invention.

Figure 18 is according to an embodiment that the present invention further provides, and supports the vertical sectional view of cellular phase I cultivation matrix probe carrier and a plurality of probe bed boards.

Figure 19 is the equipment operating function note block diagram shown in Figure 15 A-17.

Referring now to accompanying drawing,, Fig. 1 is apparatus structure and the operation chart that is used for plant growing and growth according to an embodiment of the invention.It comprises base 2, and on base 2, the upper end that 4, two garden posts 4 of two garden posts are installed is regularly connected by cross member 6.Two fixed muffles 8 can slide on garden post 4, and fix its position with tight loop 10.Sealable, bipartite container 12, the most handy stainless steel manufacturing of its underpart standing part 12a, and amplify and to be illustrated in Fig. 2, can see that it comprises the following chamber 14 that a groove forms, as shown in the figure, groove is a square-section basically.

The edge frame 15 of chamber 14 has the sealing flange 16 of edge of a knife shape, is looped around on the periphery of chamber 14.In center near 14 bottoms, chamber, the first rubber tube connector 18 that is made of discharge outlet 19 is arranged, in that there is the second rubber tube connector 20 that is made of air intake 21 14 centers from the chamber slightly, first connector is connected with sewer one class facility with rubber tube, and second connector is connected with compressed air gas source with rubber tube, and two tubes is all controlled by spring clip or valve (not shown).

The top 12b of container 12 is shown in Fig. 3, and it is the moveable part of container 12, comprises the last chamber 22 that is formed by the square opening cross section, and this chamber is topped tightly by the transparent cover plate of being made by lucite or other similar material 24.

The periphery of the edge frame 26 of chamber 22 also is provided with the sealing flange 28 of edge of a knife shape, and is identical with the sealing flange of container bottom 12a.Two screwed holes 30 and 32 lead in the chamber 22, and first screwed hole 30 is used for connecting valve 34, controls the influx of above-mentioned propagulum/liquid mixture with this valve.And second screwed hole 32 is connected with air reducing valve 36, and its purposes will be understood vide infra. Valve 34 and 36 is in Fig. 1 and Fig. 4 explanation.

The top 12b of container is slidably mounted on the garden post 4.Simultaneously, it has just constituted support 37(Fig. 1) lower cross member.Support 37 comprises two root posts 38, and column 38 fixedly is connected on the lower end of support 37.The piston rod 42 that is installed in the cylinder 44 on the cross member 6 is received upper cross member 40(Fig. 1) on, by driving cylinder 44(as shown in Figure 1), support 37 can raise or reduce, thereby, the top 12b of container just near the bottom 12a of container shown in Figure 6, will further specify below.

Fig. 4 is the diagrammatic top view of container top 12b, and is identical with the sketch of container bottom 12a.

Phase I is cultivated matrix 46 and bracket flange 48, as shown in Figure 5.Another view of this cultivation matrix is drawn at Fig. 6, will be illustrated hereinafter.

Fig. 6 illustrates the container 12 of two parts formation up and down, and during sealing, promptly container is in the operating position, and by driving above-mentioned cylinder 44,12b moves down with container top, and aims at the flange 48 of cultivating matrix 46, like this with regard in the chamber 14 under inserting.Sealing flange 16 and 28 purposes are just clear at this; Because flange 48 is quite sharp keen, they are pressed into the surface of nylon flange 48 a little, just can produce good sealing effectiveness.

Referring again to Fig. 6, sprinkler 50 generally comprises the porous of sintering, average pore size is 35 microns a metal parts, its purposes provides a minimum air bubble energy steady flow by above-mentioned propagulum/liquid mixture, with this, guarantee that the brood body in liquefied mixture evenly distributes, and will be described hereinafter.

Generally speaking, the preferred methods of one embodiment of the present of invention is as follows:

Close through sterilization and remain on the container 12 of sterilization under the atmosphere, by driven plunger it is closed and seals, the inlet valve 34 of container top 12b is opened, with certain amount aseptic vegetable material and liquefied mixture are pumped into container 12, container is bordering on is full of, close scavenge port 19, open air valve 36 simultaneously, now liquid mixture is full of the body of cultivation up and down of entire container 12, and vegetable material is cultivated matrix 46 ' be fixed on indoor.

Under 2-3 atmospheric pressure, aseptic small air bubble is by sprinkler 50 ejections, and rise reposefully, fully mix the vegetable material in the screen cloth 46 top liquid, mix approximately after 20 seconds, close air valve 36, open bleed valve, allow liquid discharge from container 12 at leisure, the vegetable material that stays is evenly distributed in to be cultivated on the matrix.Then, by chamber 12b on the drive unit rising container, open container 12, under aseptic condition, remove and cultivate matrix 46 and its support 48, and be placed on the top of culture vessel, fully contacting with the medium base plate, this structure helps tissue growth and develops into plantlet.Then, by device plantlet is transplanted in the transplanting dish, the implantation technique that is adopted is described hereinafter.

Referring again to accompanying drawing, Fig. 7 and Fig. 8 are the structural representations of cultivation matrix of the present invention.The most handy sterilizable material is made, and as the plastics of nylon and so on, it comprises and is essentially square-section and open-topped little culturing room 102 of arranging as grid, the wall of little culturing room is an entity, and shrink downwards, therefore, little culturing room is an inversion pyramid.Per two adjacent walls of per two adjacent little culturing room 102 converge at the upper surface of cultivating body, and form sharp keen edge, as Fig. 8 represents.The upper surface of cultivating matrix is provided with a plain-straight-face flange 104, is assemblied on the tissue culture vessel, and in the time of hereinafter will further discussing, will be clear.

In Fig. 9 and 10, show the structure of the little culturing room in the culture vessel 106 in the embodiment of the present invention.The top, chamber on the end 110 of culture vessel 106, is provided with supporting member or base plate 112 as grate by top cover 108 sealings, about 1 centimetre high, places nylon screen 114 thereon.As mentioned above, this nylon screen allows medium to pass through, but mesh is small enough to tissue culture is retained on the screen cloth 114 in each little culturing room 102, and the size of mesh can adopt the 250-4000 micron, is preferably the 500-700 micron.

In cited embodiment, screen cloth 114 is independently members, but also can become an integral body to screen cloth 114 with the medium system, for example, screen cloth 114 is compressed on the bottom surface of cultivating matrix.

Referring now to Figure 11-14,, these figure illustrate the transplantation device structure and the operation of an optimum implementation of the present invention.

Referring now to Figure 11 and 12,, transplantation device comprises support 180, be installed in the travelling carriage 201 on the support 180, one or more phase I cultivate matrix 202 and are installed on the travelling carriage 201, edge along travelling carriage 201, a belt cycle carrier is set, or one can support transplanting dish 204 or one row be equipped with fertilizer the bed 203.

Above travelling carriage 201 and conveyer or bed 203, a mobile bridge shape stand 205 is arranged, configuration is with the carrier 206 of a plurality of probes 207 on stand 205, and probe extends vertically downward from carrier, and moving of stand provided by pneumatic system, and Figure 11 and 12 does not draw.Carrier 206 can move around between the top position of platform 201 and conveyer or bed 203 along predetermined X-axis, and also can move up and down along giving fixed Z axle, and can be away from travelling carriage 201 and conveyer or bed 203.

In this embodiment, cellular cultivation matrix 202, each is divided into the culturing room of 256 open types, shown in plane, arranges by 16 * 16.Each transplanting dish 204.Or be similar to the arrangement of fertilizer bed, generally have 256 planting locations, alignment area by 16 * 16.The length of side of cultivating each culturing room of matrix 202 is about 5mm, and therefore, the planting location in the transplanting dish is with about 20 millimeters spacing arrangement.

Therefore, can know that from cultivate matrix 202 growing plants is being transplanted to transplanting dish 204, plant just must be moved 4 times distance on X, Y both direction.Use probe 207 systems to finish and extract plant, and they are transplanted to the fixed position of coiling in 204 from cultivating matrix 202.

Probe 207 is arranged line upon line with lattice-shaped, and the distance between axles that makes progress each other is the same with the center distance of the planting location of wanting in the transplanting dish 204 basically.Therefore, probe is by grid-like arrangement distance between axles about 20 millimeters of spacing on X, Y direction.

The row who expresses 4 row's probes 207 in Figure 11 establishes the position.Yet in fact, representational embodiment is a probe 207 with 4 * 4 arrangement.

Shown in Figure 13, cultivate matrix 202 to one and overlay on the part card of transplanting dish 204, in fact do not produce this situation, only be for trasplanting method is described here.256 cell labels cultivating matrix 202 are that " 1 " arrives " 16 ", divide 16 groups, every group of 16 culturing room.16 planting locations in transplanting dish 204 and corresponding 16 positions of probe 207 are by position 201 expressions of 16 cells of cross-hatching.

By platform 201 move and row's probe along the displacement of X axis, make 16 culturing room " 1 " perpendicular alignmnet under each of 16 probes 207.Being extracted in the plant (will explain hereinafter) in 16 culturing room " 1 ", is that each probe 207 is aimed at 16 planting locations in the transplanting dish 204 respectively vertically downward.

Realize probe carrier 206 along X axis suitably move and conveyer or bed 203 along Y-axis to suitably move and can accomplish.Then, with plant planting at the transplanting dish or transplant in the fertilizer of frame 204, will be hereinafter in advance with explanation.Carrier 206 with probe 207 is moved back into the top position of cultivating matrix 202 on the platform 201 again along X axis then, moves 16 probes 207 and 16 culturing room " 2 " perpendicular alignmnet of arranging.

Then, plant is carried by carrier 206 and probe 207, and with 16 relevant positions " 2 ", with plant planting at the transplanting dish or transplant in the fertilizer of frame 204.Like this, the suitable of platform 201 moved, and move and the carrier 206 of conveyer 203 have just been finished along the displacement of X-axis.Equally, the plant planting in per 16 culturing room's groups of 3 to 16 is arrived in " 16 " in the relevant position " 3 " of transplanting dish or transplanting frame 204.

In this way, the plant transplanting from whole 256 culturing room of transplanting dish 202 is in 4 double-lengths wide transplanting dish or row's frame 204.Suitably moving of platform 201, the mobile and carrier 206 of conveyer or bed 203 all is automatically to carry out along the displacement of X axis.And can any mode easily realize.For example, mobile available by little processing or computer-controlled electric device or the realization of electro-pneumatic device.In addition, hand gear that also can a kind of simple form is realized suitably moving of platform 201, conveyer or bed 203 move and carrier 206 along the displacement of X axis.

Referring now to Figure 14,, Figure 14 shows the platform 201 of an optimum implementation, platen 210 with band square groove 211 supports cellular cultivation matrix 212, this cultivation matrix be divided into 16 * 16 as the open type culturing room that grid is arranged, the bottom of these culturing room is made of general gauze or screen cloth 213.For example, cultivating matrix 212 and screen cloth 213 can be a kind of plastics rolled-up stock, and a chamber in these culturing room is with 214 expressions.

The structure of these culturing room such as above-mentioned relevant Fig. 5-7, and contain a large amount of growth mediums and micropropagation plant or seedling.Configuration carrier 206 above platen 210 is provided with the probe 207 that a row hangs on carrier 206.As mentioned above, the spacing that requires in this arrangement setting with lattice-shaped and the transplanting dish is consistent.

For example, probe 207 is with 4 * 4 grid-like arrangement, and wherein, every probe 207 is arranged on the top of cultivating per the 4th corresponding culturing room 214 in the matrix 212.There is shown a probe 207 analysing and observe.

Below platen 210, a fixed head 215 is set, on this plate,, the air nozzle 216 of grid-like arrangement is set with interval corresponding to probe 207.When carrier 206 is positioned at platen 210 tops, as shown in figure 14, every probe 207 perpendicular alignmnet above corresponding nozzle 216.Conduit system 217 in nozzle 216 and the plate 215 is connected, and by this system, compressed air is added on each nozzle 216 simultaneously.To be illustrated hereinafter.Platen 210 makes the 4th culturing room 214 perpendicular alignmnet nozzle 216 and probe 207, mode as described in Figure 13 respectively along X and Y both direction.

When adjustment platen 210 makes culturing room 214 with nozzle 216 perpendicular alignmnets, as shown in figure 14, the end of each culturing room 214, is tightly near nozzle 216, thus, upwards spray compressed air by nozzle 216, the plantlet in the culturing room 214 is lifted from the awl wall bottom of each culturing room.

Each probe 207 has a frustum chamber 218 that makes progress at its lower end or the place of chewing.Therefore, when carrier 206 axially continues to reduce downwards along Z, the chamber 218 of probe, as showing, will be positioned at the top of culturing room 214 tightly, and only for a probe, with dashed lines 218 in Figure 14 ' expression.

The tubulose prolongation 220 of probe 207 is made of chamber 218 separately, and in chamber 218 upper ends air vent 219 is set.The extension 220 of tubulose is sealed above air vent 219 by upper end fixing pogo stick or push rod 221.As shown in the figure, by the generation effect between the upper end of extension 220 and the piston head 223 on the push rod 221 of spiral back-moving spring 222.

Therefore, when probe 207 reduces, when the lower surface of chamber 218 touches the upper surface of cellular cultivation matrix 212, and a blast injection of ejection is to the lower surface of corresponding culturing room 214; Plantlet in each little culturing room will be pushed out culturing room 214 up, transfer to each chamber 218, and the air that chamber 218 is discharged is discharged by exhaust outlet 219.Most growth medium will oppositely be wedged in the frustum of chamber 218, and like this, the plant of various kinds size is left with in chamber 218 separately.

Then, carrier 206 axially raises along Z, and moves to the top position of transplanting dish or frame 204 along X axis.In such operation, plant still remains in separately the chamber 218, and is oppositely to wedge in the frustum of chamber 218.

The main body 224 of each probe 207 also is a tubulose, and is connected with conduit system 225 on the carrier 206.Compressed air is added conduit system 225 with on the push rod head 223 that acts on each probe, down promote push rod, as dotted line 221 ' expression.Thus, the plant in the chamber 218 is separately sprayed in the fertilizer of transplanting dish or frame 214.Yet, before this, reduce carrier 206, the lower end of chamber 218 is imbedded in the fertilizer, or be inserted in the recess of doing in advance in transplanting dish or the frame 204 inherent fertilizer, so that plant is sprayed plantation by push rod.

Carrier 206 moves to transplanting and coils 204 top positions between position shown in Figure 14, vice versa.Moving of the rising of probe and reduction platen 210 and transplanting dish 204 all is under the controlled condition of control device, realizes with electric device or electro-pneumatic device.As mentioned above, for example, adopt microprocessor or calculator, operate and mobile process.Equally, adopt electro-pneumatic device complete operation under the correct control program of calculator or microprocessor, compressed air sprays the plant in the culturing room 214 by nozzle 216, and the plant in the culturing room 218 is injected on the transplanting dish.In addition, above-mentioned various functions also can realize by manual operation electric device and control valve for fluids.

According to another embodiment of the invention, use spring 222 in order to get rid of, suction is added to conduit system 225, in correct time, improve push rod 221.

Water is injected each probe,, the plant in the chamber 218 can be gone out by the water of ejection.

Referring now to Figure 15 A-19,, this figure has illustrated the structure of transplantation device of the present invention and another embodiment of operation.Transplantation device comprises a support 300, and the bridge shape platform assembly 302 that an X-Y moves is installed on this support.On travelling bridge shape platform assembly 302, carrier 306 is set, the pneumatic system that many probes that extend vertically downward 307 is arranged and supply gas on carrier 306.In Figure 15 A and 15B, do not draw.

In this embodiment of the present invention, carrier 306 along give fixed X and Y-axis to, between the position above fixed station 308 and conveyer or the bed 310, move back and forth, and also can move along the predetermined axial high and low of Z, also can be away from fixed station 308 and conveyer or bed 310.

Generally speaking, the main distinction between the embodiment of the embodiment of Figure 11 to 14 and Figure 15 A to 19 is as follows: in the embodiment of Figure 11 to 14, transplanting is to realize along two axial displacements by cultivating foster matrix and transplanting dish and carrier.And in the embodiment of Figure 15 A to 19, do not need move to cultivate matrix and transplanting dish, all finish transplanting X, Y and Z-direction mobile by carrier.Except that above-mentioned, the structure of the transplantation device of Figure 11-14 and the description of operation also are applicable to the description of the embodiment of Figure 15 A-19, will not repeat at this.For simplicity's sake, identical reference number is as the identical parts of expression.

Can see that from Figure 15 A and 15B mechanical device and electrical installation also are installed on the support 300, for example, comprise compressor 320, vavuum pump 322 and control circuit 324, and the operator controls the control panel 326 of transplanting.

Referring to Figure 17, except being provided with the nozzle 216 in each culturing room 214, platform 308 is all similar in relevant various aspects with the platform 210 among Figure 14, and has above described.Therefore, as pointing out above, adopt same reference number to represent the component parts of platen 308.As everybody knows, for desirable nozzle mode of operation is provided, the arm that is connected with each nozzle can be set suitably.

Carrier 306 is set above platen 308, on carrier 306, the sagging probe 307 of a row is set.As mentioned above, these probes are as the arrangement of grid, and the spacing that requires with the transplanting dish is identical.

In the embodiment of Figure 15 A-19 was described, platen 308 can not move, and would rather carrier 136 be arranged on the X-Y plane, so that make all the 4th culturing room 214 and every probe 307 perpendicular alignmnets.Provide desired position by proper handling x-Y travelling bridge shape platform 302.

When carrier 306 was in the appropriate location, the culturing room 214 that probe 307 perpendicular alignmnets are selected opened the nozzle below these culturing room, and ejection compressed air boosts indoor 214 plant by the following awl wall of each culturing room.

Each probe 307 in its lower end or the place of chewing a frustum chamber 318 is up arranged therefore, when carrier 306 is axially fallen along Z, the chamber 318 of probe, as shown, will be near culturing room 214, only with regard to certain nozzle, dotted line is represented among Figure 17 318 '.

The tubulose extension 320 of probe 307 constitutes chamber 318 respectively, and in chamber 318 upper ends suction hole 319 is arranged.Suction hole 319 communicates with suction chamber 373 in being arranged on part carrier 306, and suction chamber 373 is connected with vacuum pipeline 375, is connected with vavuum pump 322 at last (Figure 15 A).

Do the time spent when spirality back-moving spring 322 produces in the upper end of extension 320 with between the piston head on the push rod 321 323, promptly above suction hole 319, tubulose extension 320 is sealed, as shown in the figure by the push rod 321 that is fixed on the upper end.When the lower end that probe 307 is reduced to chamber 318 gets close to the upper surface of cellular cultivation matrix 212, blast injection to the lower surface of corresponding culturing room 214, is evacuated chamber 318 through suction hole 319.As a result, the plant in each culturing room will upwards be pushed out culturing room 214, and be transplanted in separately the chamber 318.

Then, carrier 306 axially raises along Z, moves to the top of transplanting dish or frame 204 along X-Y plane.In this operation, it is in the wedged frustum wall that plant is retained in separately the chamber 318 respectively.

The main body 324 of every probe 307 also is a tubulose, and is connected with compressed air arm 326 in carrier 306 through conduit 325, through suitable conduit 327, is connected with compressor 320 (Figure 15 A) again.

Compressed air is added to compressed air arm 326, act on every probe push rod 321 323 on, and down promote push rod 321, as with dashed lines 321 ' expression.Thus, the plants in the chamber separately 318 are sprayed in the fertilizer of transplanting dish or frame 204.Before this, reduce carrier 306, contact with the lower surface of chamber 318, imbed in the fertilizer be inserted in the transplanting dish or frame 204 in the fertilizer nest in so that the plantation of the jet-action realization plant by push rod.

Carrier 306 moves to transplanting from the position of Figure 17 and coils certain position, 204 tops, and vice versa.Probe increases and reduces, by electric device, and pneumatic means or electro-pneumatic device, the requirement that should control and the control device that is provided with.For example, control with microprocessor or calculator compressed air is added on the corresponding nozzle 216.

The microprocessor preferably that is suitable for this respect application is 2800 types by the control technology company manufacturing of U.S. Westboro Massachusets01581.Travelling bridge shape platform 302 generally comprises X travelling bridge shape platform, model MI1224 and y travelling bridge shape platform, model MI80606, they are by Linear Industries, Lintech Division of 3440 San Fernando Road, Los Angeles, California 90065 makes.Adopt the bar shaped bearing to make the rising or the reduction of probe 307.For example, model THKIM13 is by THK America of 1715 EIK Grove Village Lllnois, 60007, U.S.A makes and model MBD25/25 piston, and by Soltam of P.O.B371, Haifa Israel makes.

Equally, provide compressed air, plantlet is sprayed from culturing room 214, be injected in the transplantation device, and form vacuum through suction hole 319 by the plant of conduit 325 with chamber 318 by nozzle 216.This operating process is that the pneumatic or electro-pneumatic device of calculator or microprocessor programme-control is in accordance with regulations finished.In addition, above-mentioned various operations also can realize by manual operation electric device and control valve for fluids.

Referring to Figure 18, it has described another embodiment of Figure 17 device.Wherein probe is improved, the water of ejection can be sprayed on the plant of plantation.Unless certain illustrated is arranged, otherwise the same parts of Figure 18 and Figure 17 device all adopt same reference number.

In the device of Figure 18, the extension 320 of probe 307, each all is connected with the water inlet 381 of water supply conduit 383.Each all has the passage 385 of water push rod 321, and passage 385 connected surfaces portal 387 and push rod 321 ends outlet 389 on the 381 1 side push rods of hole.Can know, when push rod 321 is in its extended position, promptly during the injection plant in the planting process, by hole 381 admission passages 385, discharge by outlet 387 at last from the water of pipeline 383.

Therefore, if supply water by pipeline 383, when plant when chamber 318 spray by water, this plant can obtain a certain amount of water, thereby knows, timing adds entry, can suitably be controlled by calculator.In addition, the extension tight engagement of push rod 321 and probe 320 is so that play a valve, extension by push rod 321 provides the needed water yield, like this, inlet 381 and 387 just can be controlled the water yield, also with regard to the water yield of the supplying duct 383 that do not need to computerized control.

Referring now to Figure 19,, that its is represented is the operational block figure of the embodiment of the present invention of Figure 15 A-17 description.Operation is in zero-bit from carrier, for simplicity, considers some positions of being failure to actuate.

When the operator uses control panel 326(Figure 15 A) when given one " beginning " instructs, vacuum system reaches suitable vacuum, compressed air gas source is transferred to suitable pressure, carrier 306 moves to the initial load position, be purposes of simplicity of explanation, can think to be arranged on the position of Figure 13 culturing room " 1 " at the probe on the carrier 306 307.

When this starting position, probe axially reduces along Z, opens the nozzle 216 under the culturing room, so that plantlet is sprayed in the probe chamber 318, then, again along the Z probe that axially raises, carrier moves to the implant site of transplanting dish top along X-Y plane.

When reaching implant site, probe reduces along the Z axle, removes vacuum, forces push rod downward by compressed air, and plantlet is sprayed.After this, carrier moves to another position of cultivating the matrix top along X-Y plane.For example, probe is placed on the culturing room top of label " 2 ", and push rod is retracted, and forms vacuum again, and following plantlet just inserts in the sound end, so back and forth back and forth till the plantlet in the cultivation matrix has been transplanted.

The professional and technical personnel understands that the present invention is not subjected to above-mentioned description and each illustrated restriction, and scope of the present invention is determined by following claim.

Claims (20)

1, the system of a plant growth and growth comprises:

But a cellular cultivation matrix of phase I comprises the support bottom surface of a seepage and the parietal layer structure of an independent culturing room of device first row on above-mentioned bottom surface, and each culturing room is spaced apart by first spacing culturing room adjacent with first row,

The second stage that the independent plantlet of second row vitellarium is housed is cultivated supporter, separates by independent cultivation region and second being arranged contiguous vitellarium greater than second spacing of first spacing,

A kind of being used to provide on cellular cultivation matrix of above-mentioned phase I with evenly the distribute device of propagulum of medium,

A kind of row from first transplanted single plantlet in the culturing room, and they is transplanted to second stage cultivates the corresponding mechanical device of vitellarium separately on the supporter.

2, the step of a plant growth and growth method is:

A cellular cultivation matrix of phase I is set, the parietal layer structure that but this matrix comprises the support bottom surface of a seepage and independent culturing room is arranged in body plan first above the support bottom surface, little culturing room adjacent among above-mentioned these the independent little culturing room and first row is separated by first spacing

Setting is cultivated supporter by the second stage that the independent plantlet of second row vitellarium is constituted, by second spacing, vitellarium adjacent among the above-mentioned independent vitellarium and second row separated greater than first spacing,

Together with medium the plant breeding body is evenly distributed on the cellular cultivation matrix of phase I,

Plantlet with being grown in the first row culturing room shifts out in independent culturing room by mechanical way, they is transplanted to second stage cultivates corresponding vitellarium separately on the supporter.

3, on a kind of supporter and screen cloth that is installed in the airtight container that upper and lower chamber constitutes, the method for the propagulum that evenly distributes, the step that comprises is:

The liquid mixture that will contain the Plant Tissue Breeding brood body is sent into upper chamber, and feeding bubble stream mixes the brood body in the liquid mixture of screen cloth top in liquid mixture, and forms a kind of basic suspension uniformly,

Allow that above-mentioned basic suspension uniformly is discharged to the bottom chamber of said vesse by above-mentioned supporter and screen cloth, thereby on supporter and screen cloth, make the suspension brood body deposition that evenly distributes.

4,, it is characterized in that providing a kind of even distribution step according to the said method of claim 2:

The place settles above-mentioned cellular cultivation matrix of phase I in the middle of the chamber of the upper and lower part of container,

The liquid mixture that will contain the Plant Tissue Breeding brood body is sent into upper chamber,

In said liquid mixture, feed bubble and flow stirring and mixing, the brood body in the liquid of the basic top of cellular cultivation of phase I, and form a kind of uniform basically suspension,

Allow that basic suspension uniformly is discharged to container bottom cavity by cellular cultivation matrix of phase I, distributes thereby on phase I cultivation matrix the suspension brood body is produced evenly.

5,, it is characterized in that being used to provide equally distributed device and comprise according to the said device of claim 1:

A kind of container that can seal that is divided into upper and lower chamber by phase I plant culture body, upper chamber comprises that an introducing contains the controlled import of the liquid mixture of Plant Tissue Breeding brood body, a lower chamber controlled Pai Kou of configuration and the air intlet that leads to the bubble distributor that can control.

6, a kind of culturing room that is used to cultivate the Plant Tissue Breeding brood body comprises as the regularly arranged real wall culturing room of grid, its size with the plantlet that can keep being cultivated in each culturing room be in each other substantially evenly, fixing position.

7,, it is characterized in that the inverted frustum that is shaped as of culturing room according to the said culturing room that is used for cultivating the Plant Tissue Breeding brood body of claim 6.

8, comprise according to claim 6 or 7 said a kind of culturing room that are used for cultivating the plant tissue brood body: in the culturing room bottom screen cloth and support component are arranged, size of mesh with can allow medium by and can hold back tissue propagation's body from the teeth outwards.

9, cultivate according to the said plant species of claim 1 and development system is characterized in that cellular matrix of phase I comprises the real wall culturing room that picture grid is regularly arranged, its size can be so that the plantlet of cultivating in each culturing room can be in the position of evenly fixing each other.

10, cultivate and development system according to the said plant species of claim 9, it is characterized in that the inverted frustum that is shaped as of each culturing room.

11, cultivate and development system according to claim 9 or 10 said plant species, but the liquid supporter bottom surface that it is characterized in that above-mentioned seepage comprises the screen cloth and the support component of culturing room bottom, size of mesh passes through can allow medium, also can hold back the tissue culture propagating body from the teeth outwards simultaneously.

12, cultivate and development system according to the said plant species of claim 1, the width about 5 that it is characterized in that culturing room is to about 12mm.

13, a kind of germ-free plant tissue culturing equipment comprises:

The container that can seal that inner bottom surface is arranged,

Be installed in the container and the screen cloth and the supporter parts that on inner bottom surface, can promote,

Medium is filled in the container, and it is highly consistent with the height of screen cloth and supporter,

The tissue culture body be supported on the surface of screen cloth and supporter and and medium contact,

On the bottom surface of the culturing room of cultivation plant tissue brood body screen cloth and support component are set, it constitutes the real wall culturing room of the upper and lower perforation of arranging as grid, and its size is in evenly fixing position each other with the plantlet that keeps being cultivated in each culturing room.

14, be used for the cultivation body in the open type culturing room of first row's grid-like arrangement is transplanted in the device of each relevant position of second row's grid-like arrangement, centre-to-centre spacing between each position in second row's grid-like arrangement is greater than the centre-to-centre spacing of the first row culturing room, and this device comprises:

Carrier with a plurality of probes, its probe are pressed the regularly arranged centre-to-centre spacing of grid the centre-to-centre spacing with second kind of arrangement position are consistent basically,

Make carrier and first, second row culturing room produce the device of relative displacement, probe at first aimed at the first row culturing room, and then aimed at second row,

Be used for extracting the culture in first each culturing room of row and remaining on each probe, till aiming at the second row relevant position, put into the device of second kind of row's relevant position at last.

15, from cellular cultivation matrix of phase I, by the device of in the regularly arranged culturing room of grid plantlet being transplanted to each planting location of second stage cultivation supporter, wherein the centre-to-centre spacing of the kind seated position of second grid is greater than the centre-to-centre spacing of the culturing room of first order, ratio equals one times substantially, and this device comprises:

Carrier with a plurality of probes, consistent with second kind of arrangement position basically by the distance of the probe core in the grid-like arrangement,

Be used for being implemented in the device of relative displacement between carrier and first, second kind arrangement, at first make probe alignment first row's culturing room, and then aim at second row's relevant position,

Be used for realizing extracting the culture in first each culturing room of row, before probe is aimed at second kind of row's relevant position, culture is retained on the probe, put into the device on second relevant position of arranging at last, a plurality of probes by stationary arrangement take out plantlet in a plurality of culturing room simultaneously, be transplanted to then in the position of desired grid, the length of this position and the spacing on the cross direction are all cultivated the spacing of the culturing room in the matrix greater than the phase I.

16, according to claim 1,5 and 9 to 11 said a kind of plant cultivation and development systems of being used for, it is characterized in that comprising with the device of mechanical lifting and transplanting:

Transplant plantlet in being used for cultivating culturing room the matrix and cultivate device in the corresponding plantation of supporter to second stage from the phase I, wherein culturing room is regularly arranged by first kind of lattice-shaped, centre-to-centre spacing between the planting location of second kind of trellis arrangement is greater than the centre-to-centre spacing between the first row culturing room, ratio be substantially equal to a multiple

This device comprises:

The carrier that a plurality of arrangement probes are housed, its probe are pressed centre-to-centre spacing that trellis arranges basically with the centre-to-centre spacing unanimity of second kind of arrangement position,

Be used between carrier and the first, the second kind of arrangement, realizing the device of relative displacement, at first make the culturing room of probe alignment first order, and then aim at second row's relevant position,

Be used for realizing extracting the device of the culture in first each culturing room of row, before probe was aimed at second row's relevant position, culture was retained in the probe, puts at last on second row's the relevant position.

17, utilize tubular probe, on primary importance, the culture in the culturing room of upper and lower perforation is transplanted to the device of the second place,

Be used for continuous realization probe and constitute primary importance or the device of the second place between produce the device of displacement, make mouth of the culturing room of probe alignment primary importance, and then with second place aligning,

When probe with the mouth of the culturing room of primary importance on time, with the pressure injection of compressed air or other fluid device, the culture of culturing room is ejected in the probe to culturing room,

When the probe alignment second place, compressed air or other fluid pressure are ejected on the probe, again the culture on the probe is ejected in the second place.

18, it is characterized in that being used for realizing that the device of relative displacement comprises according to any said equipment of claim 14 to 17:

Be used for making upper carrier at least along the device of the first and second vertical base parameter displacements,

Be used for making one of first and second kinds of above-mentioned arrangements to be displaced to the device of the first and second above-mentioned vertical coordinate axles at least along one the 3rd vertical coordinate.

19, it is characterized in that being used for realizing that the device of relative displacement comprises according to any said device of claim 14 to 17:

Be used for making the device of above-mentioned carrier along the first, the second and the 3rd vertical base parameter displacement.

20,, it is characterized in that being used for realizing the device that discharges culture being discharged the device that provides liquid to discharge according to any said device of claim 14 to 19.

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