CN85108903A - Method for producing a novel anti-inflammatory agent - Google Patents
Method for producing a novel anti-inflammatory agent Download PDFInfo
- Publication number
- CN85108903A CN85108903A CN85108903.8A CN85108903A CN85108903A CN 85108903 A CN85108903 A CN 85108903A CN 85108903 A CN85108903 A CN 85108903A CN 85108903 A CN85108903 A CN 85108903A
- Authority
- CN
- China
- Prior art keywords
- milligrams
- bisabolol
- chamazulene
- opopanax
- flower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- VHHHONWQHHHLTI-UHFFFAOYSA-N hexachloroethane Chemical compound ClC(Cl)(Cl)C(Cl)(Cl)Cl VHHHONWQHHHLTI-UHFFFAOYSA-N 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 150000004001 inositols Chemical class 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- CLEXYFLHGFJONT-UHFFFAOYSA-N jervine Natural products C1C=C2CC(O)CCC2(C)C(C(=O)C2=C3C)C1C2CCC13OC2CC(C)CNC2C1C CLEXYFLHGFJONT-UHFFFAOYSA-N 0.000 description 1
- QRXOCOSLDOPPKH-UHFFFAOYSA-N jervine sulfate Natural products CC1CNC2C(C1)OC3(CCC4=C(C3C)C(=O)C5C4CC=C6CC(O)CCC56C)C2C QRXOCOSLDOPPKH-UHFFFAOYSA-N 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- UQDUPQYQJKYHQI-UHFFFAOYSA-N methyl laurate Chemical group CCCCCCCCCCCC(=O)OC UQDUPQYQJKYHQI-UHFFFAOYSA-N 0.000 description 1
- 239000003110 molding sand Substances 0.000 description 1
- NZMAJUHVSZBJHL-UHFFFAOYSA-N n,n-dibutylformamide Chemical compound CCCCN(C=O)CCCC NZMAJUHVSZBJHL-UHFFFAOYSA-N 0.000 description 1
- 150000002790 naphthalenes Chemical class 0.000 description 1
- 235000013842 nitrous oxide Nutrition 0.000 description 1
- 230000000422 nocturnal effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- MSYUMXXVCSLXMR-UHFFFAOYSA-N phenylmercury;hydrate Chemical compound O.[Hg]C1=CC=CC=C1 MSYUMXXVCSLXMR-UHFFFAOYSA-N 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 229910052895 riebeckite Inorganic materials 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229940084560 sanguinarine Drugs 0.000 description 1
- YZRQUTZNTDAYPJ-UHFFFAOYSA-N sanguinarine pseudobase Natural products C1=C2OCOC2=CC2=C3N(C)C(O)C4=C(OCO5)C5=CC=C4C3=CC=C21 YZRQUTZNTDAYPJ-UHFFFAOYSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 230000002048 spasmolytic effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000036561 sun exposure Effects 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000004577 thatch Substances 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 150000003544 thiamines Chemical class 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- -1 tribromaniline Chemical compound 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A method of producing a novel anti-inflammatory active agent: the novel agent is extracted from a novel tetraploid chamomile rich in bisabolol; it contains at least 30 mg% chamazulene and 100 mg% of alpha-bisabolol, and the content of other red bisabolol is less than 50 mg%.
Description
At pure (spring Huang) chrysanthemum (Chamomilla recatita(L.) Rauschert and Matricaria Chamomilla(L.) synonym) medicament and the cosmetic agent of making on the fresh idea preparation basis, because they have antiinflammatory and spasmolytic activity, so have many uses.In this respect, these two kinds of active substances of (-)-α-bisabolol and chamazulene have played important function.Therefore, from the agent that (spring Huang) Flos Matricariae chamomillae prepares, should make the content of these two kinds of materials higher as far as possible.
Now found a kind of use tetraploid Flos Matricariae chamomillae, produced the method for new agent with advantageous feature.
The object of the invention is for a kind of new agent with anti-inflammatory activity is provided.This new dose has been improved the content of (-)-α-bisabolol and chamazulene with it and has been celebrated.Its chamazulene content is at least 30 milligrams of %, and (-)-α-bisabolol content is at least 100 milligrams of %, and other opopanax body burden then is lower than 50 milligrams of %.In literary composition of the present invention, " other opopanax body " refers to (-)-α-bisabolol oxide A and B and opopanax oxidation of ketones thing A.
The present invention relates to the listed problem of theme, or rather, relate to result of study listed in the claim.
According to this invention, employing (-)-α-bisabolol is that the tetraploid Flos Matricariae chamomillae of the Main Ingredients and Appearance of its quintessence oil produces this new dose.
Select such plant from these (spring Huang) Flos Matricariae chamomillaes: they are (dry under 40 ℃ the temperature) in its dry flower, the minimum content of its chamazulene is 100 milligrams of %, (-)-α-bisabolol minimum content is 200 milligrams of %, and other opopanax body burden is no more than 50 milligrams of %(results flower when the tubular flower that 30-70% is arranged on the head inflorescence is open).To the plant of selecting, also can further select and breed (as to can vegetative allogamy kind cultivating its elite stand, referring to example).
The tetraploid Flos Matricariae chamomillae kind that is suitable for use as research material is: Bodegold(the Federal Republic of Germany), Pohorelicky(Czech), Zloty lan(Poland), BK-2 kind (Hungary).Can consult following document: M.Chladek and the 13rd phase of V.Kosova " Pharmazie ", 712-713 page or leaf (1958) about the description of these kinds; W.Czabajska, " DissPoznan " (1963); W.Poethke and P.Bulin, " Phaim.Zhalle " the 108th phase, 813-823 page or leaf (1969); I.Sarkany " Herb.Hungar " the 4th phase (1), 125-169 page or leaf (nineteen sixty-five).As for other situation,, be suitable for too with the tetraploid Chamomile of starting for the start observed result (seeing below) of (-)-α-bisabolol content of Chamomile of diploid.
But, be the diploid Flos Matricariae chamomillae of Main Ingredients and Appearance of quintessence oil from (-)-α-bisabolol, also can obtain tetraploid Flos Matricariae chamomillae material.Can carry out tetraploidization (genome-mutation) to this quasidiploid Flos Matricariae chamomillae.The tetraploid plant that obtains is in this way selected.In the tetraploid plant that chooses, select such plant again: the chamazulene minimum content should be 100 milligrams of % in its dry flower (40 ℃ dry down); (-)-α-bisabolol minimum content should be 200 milligrams of %; Other opopanax body burden is no more than 50 milligrams of %(results when the tubular flower that 30-70% is arranged on the head inflorescence is open).Then, also can do further to select and breeding according to the step of being introduced in the example.
Diploid (spring Huang) Flos Matricariae chamomillae beginning material can obtain from following Flos Matricariae chamomillae: originate from " Argentinian Flos Matricariae chamomillae " and (consult L.Z.Padula, R.V.D.Rondina, J.D.Coussio: the quantitative assay of quintessence oil, Germany's (spring Huang) Flos Matricariae chamomillae of cultivating in Argentina, azulene among the Matricaria Chamomilla and chamazulene total content, " Planta med " the 30th phase, 273-280 page or leaf, 1976); Also can from any other chrysanthemum, obtain, contain (-)-α-bisabolol (generally accounting for quintessence oil more than 5%) of suitable concentration in its quintessence oil.Following document has been done introduction to suitable diploid (spring Huang) Flos Matricariae chamomillae: H.Schilcher, and the quality of Matricariae aetheroleum and Flos Chrysanthemi is estimated recent developments " Planta med ", the 23rd phase, 132-144 page or leaf, 1973 years; Motl, O., M.Felklova, V.Lukes, M.Jasicova:(spring Huang) the gas chromatography analysis and the 310th phase of chemical type " Arch.Pharm " thereof of Matricariae aetheroleum, 210-215 page or leaf, 1977 years; Franz, Ch., J.Hoelzl, A.Voemel: preliminary description " Acta Hort " the 73rd phase of some colony of Flos Matricariae chamomillae and the morphology of kind and chemical feature, 109-114 page or leaf, 1978).
Another suitable Flos Matricariae chamomillae beginning material is diploid (spring Huang) Flos Matricariae chamomillae, and kind is the DEGUMILL(Deutsche Bundespatent, the 2nd, 402, No. 802).In this case, gather in the crops when having the tubular flower of 30-70% open on the Flos Matricariae chamomillae head inflorescence, and during dried, air themperature is no more than 50 ℃, selects to obtain a kind of tetraploid Flos Matricariae chamomillae, its chamazulene content can be 150 milligrams of % down to going out also.Bisabolol content only is 200 milligrams of % less, if go to select and breeding according to present this method more later on, so above-mentioned opopanax alcohol number and azulene value can also further increase.
Flos Matricariae chamomillae quintessence oil, its Main Ingredients and Appearance generally have farnesene, cochlear enol (Spathulenol), chamazulene, four kinds of opopanax body ((-)-α-bisabolols; Bisabolol oxide A; Bisabolol oxide B; Perhaps in the diploid individual plant, also can contain opopanax oxidation of ketones thing; And mix kind and the tetraploid individuality can contain several opopanax bodies simultaneously in colony) in a kind of, and spiral shell ether.Generally speaking, the material of being mentioned lumps together the quintessence oil that has just constituted 70-80%.Therefore, Main Ingredients and Appearance is the diploid Flos Matricariae chamomillae beginning material (it is over half to surpass above-mentioned one-tenth deal) of (-)-α-bisabolol, and is especially suitable.If (-)-α-bisabolol in diploid (spring Huang) Flos Matricariae chamomillae accounts for 40% of quintessence oil at least, or accounts for 90% of opopanax body at least, such diploid (spring Huang) Flos Matricariae chamomillae can be used as only Flos Matricariae chamomillae beginning material.
Especially can adopt diploid and tetraploid Flos Matricariae chamomillae colony, chamazulene content generally is at least 100 milligrams of % in its hybrid medicine, it is through after 40 ℃ of dried that (-)-α-bisabolol content is at least this spice of 50-100 milligram %(, based on the dry composition of flower).In other words,, obtain seed from the Flos Matricariae chamomillae colony of the above-mentioned type if serve as the beginning material with the diploid Flos Matricariae chamomillae, and carry out the tetraploid processing (needn't consider and/or not about the data of the bisabolol content of parental plant also can).If, so then can directly carry out following selection and breeding to the seed that is obtained as stated above with tetraploid Flos Matricariae chamomillae beginning material.
Suitable diploid and tetraploid Flos Matricariae chamomillae beginning material can be selected by the test individual plant.In general, in the colony that starts, should test 1000-10000 individual plant, so that find out the individual plant that some meet above-mentioned standard, their (-)-α-bisabolol content is higher, with this as suitable Flos Matricariae chamomillae beginning material.Then from individual plant, obtain seed, and it is carried out the tetraploid processing through selecting with conventional method.After the tetraploid processing, tetraploid plant is branched away from last liploid plant.In the tetraploid plant of gained, through suitable breeding, their flower should contain the chamazulene of 100 milligrams of % and (-)-α-bisabolol of 200 milligrams of % at least after dried under 40 ℃ the temperature, and other opopanax body burden should be lower than 50 milligrams of %.Such plant is all chosen.All tubular flowers on head inflorescence have the open growth period results flower of 30-70% also can further to select and breed, to improve following properties: the florescence unanimity, the base portion branch is even, flower portion compactness (being more suitable in mechanical harvest like this), head inflorescence is big, the stability of spice strengthens, and particularly the fragrance of flowers is strong.
Just can therefrom select such individual plant as stated above if adopt the tetraploid Flos Matricariae chamomillae at the very start as the beginning material, in its dry flower (40 ℃ dry down), should contain the chamazulene of 100 milligrams of % and (-)-α-bisabolol of 200 milligrams of % at least, contain other opopanax body that is lower than 50 milligrams of %.These flowers also should be gathered in the crops at trophophase, and all tubular flowers have 30-70% open on the head inflorescence at this moment.In this occasion, also can carry out some selection and breeding later on, in the hope of further being improved (as mentioned above) to these plant.
If in order to chamazulene and (-)-α-bisabolol content is the method on basis, selection obtains such plant (that is: the plant that meets this invention standard or cultivate out according to this invention), their chamazulene content reaches 200mg% at least, preferably reach 250mg%, (-)-α-bisabolol content is always at 300mg%, preferably reach other opopanax body burden of 400mg%(always below 50mg%), this is valuable especially so.
The tetraploid processing can be passed through chemical agent, the X-ray, and gamma-radiation, or ultraviolet are handled some part (seed, root, branch point, twig, axillalry bud) of diploid Flos Matricariae chamomillae plant, or it is organized and also can double processing to Anther Culture and reach tetraploidization; Can also pass through Flos Matricariae chamomillae plant or its some part, or their in vitro tissue, carry out high temperature even K cryogenic treatment, obtain the tetraploidization of plant.
The tetraploidization of chemicals.The chemicals that is suitable for the tetraploid processing has following several: colchicine, acenaphthene, alkaloid, as atropine (atropina), jervine, nicotinamide, and Sanguinarine, benzene derivative is got (two) benzene and phenanthrene, naphthalene and naphthalene derivatives, diphenylamines, tribromaniline, paracide, methylnaphthoquinone, first (base) naphthalene hydroquinone, salicylic acid and related substance thereof, hexachlorethane, dexoxyn (hydrochlorate); Alkyl-alkali carbamate, as isopropyl-sodium carbamate, ethyl phenylcarbamate and cacodylate (as: sodium salt), the material of convallarin one class, as: convallarin, convallatoxin and convallamarin, different growth hormone, Jamison's (phenyl mercury pyrocatechol); Organomercurial compound, as: ethyl mercury phosphate, ethylmercuric chloride, phenyl mercuric hydroxide and phenyl mercury dinaphthyl methane disulfonate, chloroform, laughing gas (N
2O) and composition thereof.Also can use oil cake, compost, cattle manure.
When adopting above-mentioned substance to handle, temperature range is 0-35 ℃.Wherein 12-30 ℃ better, 15-25 ℃ is best.Chemical treatment method is to handle seed, the stem apex of branch, root (as the root of the tip of a root or seedling), ovary, the tangent plane of leaf or stem, merismatic cell suspension, and callus culture thing.Even chemicals can be expelled to basal part of stem or axillalry bud position.When using chemicals, usually with aqueous solution, the form of the acid solution of the alcoholic solution (alcohol content generally is lower than 5%) of appropriateness or appropriateness is carried out.The pH value of the acid solution of appropriateness is between 5.5-6.5.With the lower aliphatic acid of this class of acetic acid, carry out acidization.The alcoholic solution that uses, also suitably acidify.Chemicals concentration in these solution can be between 0.01-0.5%, and is better between the 0.02-0.2%, best between the 0.05-0.1%, can use gas (for example: pressure is the 1-10 crust) under certain pressure.Processing time can be between 1-36 hour, and 2-12 hour better, and 4-6 hour best.
Be preferably in and obtain valid density in the pilot study, handle time of contact.
For example, handle, can obtain highly beneficial result with colchicine.Temperature can be between 0-35 ℃ during processing, and 12-30 ℃ better, 15-25 ℃ of the best.The method that adopts is: the seed of the material diploid Flos Matricariae chamomillae that will begin, and swelling in the colchicine solution of 0.01-0.2%, colchicine solution concentration is that 0.02-0.1% is better, 0.05% the best; Perhaps soak and sent out well-developed diploid Flos Matricariae chamomillae seedling (bottom has cotyledon) bud, Seedling order in five-seven days with colchicine solution.Solution concentration can be 0.01-0.2%, and 0.02-0.1% is better, and 0.05% is best.During a kind of method, the relative air humidity of ambient atmosphere must be up to 100% after use.The colchicine processing time can be preferably between 4-10 hour between 3-36 hour.If use seedling, it is just enough that the processing time generally reaches 10 hours.If use seed, processing time to require to reach 36 hours.
After the chemicals processing, expansible seed, the other parts of seedling or plant should be washed by water repeatedly.Sow expansible seed.Can be in the plant growing dish with root or other parts or the treated plantlet of transplant of seedling, can grow up to plant (as: can be placed in the greenhouse and grow, 18 ℃-25 ℃ of day temperatures, be 10-16 ℃ night) through the seed handled like this or seedling.Selecting those pollen sizes is 1.5 times of beginning materials.Or somatic chromosome reading is 36 plant.If the other parts (ground or under ground portion) to plant are handled with chemicals, only to the longer twig of plant from handling, root or flower or seed carry out the tetraploid inspection so later on.If bud or axil are handled with chemicals, so after only to the bright branch, the flower that grow out from above-mentioned axil or bud or be more preferably the seed that forms thus, carry out chromosome counting.
Can measure the size and the chromosome reading of pollen by method used in " example 3 ".
Carry out tetraploidization with radiation treatment.This processing is being carried out on the seed or on the tip of a root.Temperature range is 0-35 ℃, and 10-30 ℃ better, and 15-25 ℃ best.Total radiation: the 5-50Krad(kilorad).Use gamma-radiation or X-ray better.Suitable ultraviolet, its wavelength can be between 400-300nm, and 350nm is better.
To the further processing method of the some parts of the plant of radiation or plant, identical with the processing method of using the plant after chemicals is handled.
Use high temperature and K cryogenic treatment.
Suitable high temperature is between 33-50 ℃, and 42-45 ℃ better.Adopt these Temperature Treatment expansible seed, seedling, twig and separate living tissue.Processing time: 1-48 hour, 12-24 hour better.
Suitable low temperature is between 0-5 ℃, and 0.5-4 ℃ better, and 2 ℃ best.Adopt this Temperature Treatment expansible seed, seedling, twig and separate living tissue.Processing time: 1-100 days, 20-40 days better.
The further processing of the plant of treated mistake or plant part, the plant method after chemicals is handled is identical with using.
Anther Culture (produce monosome group plant from flower pesticide, double with chemicals or ray subsequently).
Gather the tubular flower that is in closure state from the plant that blooms, at this moment flower pesticide is in period before the first pollen mitosis.With micromanipulator with flower pesticide taking out on the bud, and it is received in (the seeing Table 1) culture dish that contains the Nitsch-Nitsch culture medium.Culture dish is placed in the culturing room of 16 hours irradiations every day (the photophase temperature: 28 ℃, temperature is 20 ℃ in the dark), about four are after date, and flower pesticide begins to split, and plantlet begins growth.As the parent is diploid, and then plantlet is a monoploid, is tetraploid as the parent, and then plantlet is a dihaploid.After root forms, it is planted into flowerpot, soil adopts garden soil, then is placed on it is bloomed again.Though the plant of these monoploid or dihaploid is sterile, available chemicals as colchicine, are handled stem apex, root or stem, just make its polyploidization with the generation plant of isozygotying, and can further breed by seed then.Further handle with use chemicals processing (as: colchicine processing) after the method for plant identical.
Table 1
Nitsch-Nitsch culture medium (" science " 1969)
Mg/litre
KNO
3950
NH
4NO
3720
MgSO
4×7H
2O 185
CaCl
2166
KH
2PO
468
MnSO
4×4H
2O 25
H
3BO
310
ZnSO
4×7H
2O 10
Na
2MoO
4×2H
2O 0.25
CuSO
4×5H
2O 0.025
5 milliliters of following solution: in 1000 ml solns, contain the disodium EDTA of 7.45 grams, contain the FeSO of 5.57 grams
4* 7H
2O
Inositol 100
Glycine 2
Nicotinic acid 5
Pyridoxin hydrochloric acid 0.5
Thiamine hcl 0.5
Folic acid 0.5
Biotin 0.05
Sucrose 20g
Nutrition base (agar) 8g
Acetic acid 0.1
The pH value of culture medium is transferred to 5.5
Tetraploid Flos Matricariae chamomillae plant as the preamble introduction, select by pollen size or Chromosome number, and select such plant thus again, its chamazulene minimum content is 100mg%, the bisabolol minimum content is 200mg%, other opopanax body (especially bisabolol oxide) content will be lower than 50mg%(based on dry flower, with reference to " example 1 ").
Take the cephaloid that all have been bloomed,, be placed in have illumination in 14 hours every day at least the greenhouse of (day temperature is 18-24 ℃, and nocturnal temperature is 12-14 ℃) withered until it with the plant of selecting like this.After spending for four weeks, the head inflorescence collection that maybe will disintegrate that all are withered is to reclaim seed.Be under 20-35 ℃ the condition, it to be carried out dried in air themperature, like this, just can have obtained the breeding seed of this type Flos Matricariae chamomillae of being discussed.
Also have some standards below, it is used to can be this system of selection:
A) bloom approximately at the same time.
B) the base portion branch is even, flower portion compactness (10cm, preferably 5cm).
C) head inflorescence is big, and the about 30mm(of its external diameter can be between 20-40mm) 25-35mm is better.
If reach standard a), b) and/or c), can make flower and spice high yield, and be more suitable for using mechanical harvest.
If, so in kind carry out as the beginning material with known tetraploid Flos Matricariae chamomillae, if necessary, select once more and breed as above-mentioned selection.
In order to obtain such Flos Matricariae chamomillae, its chamazulene content is at least 100mg%, (-)-α-bisabolol content is at least 200mg%(based on exsiccant fresh idea), and flower yield height, be more suitable for using mechanical harvest, we recommend following method: to the plant (as mentioned above) that chooses, transplant a cutting asexual propagation 3-5 more than generation, the above-mentioned standard of feeling the pulse with three fingers simulataneously also can be by additional standard a) to c) select.
To be undertaken cloneization by the plant that these Standard Selection are come out.From each clone plant, reclaim seed; Again by above-mentioned chamazulene and bisabolol or according to additional standard a) to c) minimum content selects the plant of being obtained.Obtain seed in the plant of selecting thus.
According to above-mentioned standard: the chamazulene minimum content is that 100mg% and (-)-α-bisabolol minimum content are that other opopanax body of 200mg%(is lower than 50mg%) and also can be according to additional standard a) to c), sow and select, reclaim seed subsequently, such process should repeat 3-5 time.
Also have the program of another sowing, selection (can carry out cloning) and recovery seed, the seed that obtains at last is exactly the seed that is used for breeding Flos Matricariae chamomillae of this invention defined.
Can be propagated from cuttings by following method:
In order to be propagated from cuttings, original plant (maternal plant clone) must be placed under the condition of short-day, generate the brachyplast of no bud, this does not have in the winter time in the greenhouse of any additional optical photograph and carries out, or carry out in the controlled environment chamber, illumination every day 6-10 hour, 8 hours are better, temperature is between 10-15 ℃, and 12 ℃ better.Leaf, twig, especially brachyplast (side shoot) are transplanted a cutting, and are suitable for cloning, perhaps or rather, are fit to breeding.Such cutting is (about 100% relative air humidity) in saturated atmosphere, and between temperature 12-18 ℃, 15 ℃ better, and light application time is between 12-16 hour, and better under such condition, they can be taken root in 14 hours.
Cultivation matrix can be used peat and husky mixture, and ratio is 1: 1, although also available pure quartz sand, asbestos, clay, water, hybrid particles, peat, clay, water hybrid particles and similar substance thereof.
Can have in order to the soil of sowing: garden soil, heavy loam in the humus type; Loam or humus molding sand loam.Can be in the greenhouse, so the open air is sowed.Rudiment of plant and growth temperature can be between 12-24 ℃, and 18-20 ℃ better.If sow out of doors, sowing season, (JIUYUE, October) was better in autumn, also can be in spring (March, April).This sowing time is suitable for all in question cultivation areas (as the Northern Hemisphere, the moderate area of the temperature in the subtropical climate area).
It is particularly advantageous using following process conditions: at the chamazulene minimum content is 100mg%, (-)-α-bisabolol minimum content is 200mg%, other opopanax body (especially bisabolol oxide) content is lower than all numerical value of 50mg%(based on exsiccant head inflorescence) tetraploid Flos Matricariae chamomillae plant in, should select such plant, bloom approximately at the same time; The base portion branch is even, and the layer of blooming is narrow, is about 10cm; The beginning locusta is big, and outer dia is about 30mm.
The plant that is selected breeds by broadcasting the method for inserting, and selects 3-5 more than generation.The above-mentioned standard of feeling the pulse with three fingers simulataneously is selected.The plant of selecting is carried out asexual propagation (cloneization), then put and make it withered together, reclaim seed.To the plant that from these seeds, obtains, select successively by above-mentioned standard.Sowing then selects to reclaim seed, and such process repeats 3-5 time.
According to the present invention, be used for producing the Flos Matricariae chamomillae of agent having ahtiphlogistic activity, belong to the cultigen of pure Flos Matricariae chamomillae, its vegetable formal name is Flos Matricariae chamomillae Matrhcaria Chamomilla(L.) (with Chamomilla recutita(L.) synonym, Rauschert).Take from the material of this Flos Chrysanthemi, carry out dried being no more than under 70 ℃ the temperature, flower need be gathered in the crops at growing period, has this moment the tubular flower of 30-100% open on the head inflorescence.This material contains the Flos Matricariae chamomillae of 30mg% and (-) of 100mg%-α-bisabolol at least, and the content of other opopanax body is lower than 50mg%.
If further grow at flower, be that whole tubiflorous 100% on the head inflorescence all opened (as: blooming the Sheng phase), the results Flos Chrysanthemi, and (perhaps) results after the flower dried time temperature be higher than 40 ℃, the content of (-) so-α-bisabolol and Flos Matricariae chamomillae can reduce.Because dried temperature height, and postpone between (perhaps) results, can greatly make the degraded of azulene and bisabolol.
Press the Flos Chrysanthemi (gathering in the crops as stated above) of the technology acquisition of this invention defined, in drying baker, temperature is under 40 ℃ of conditions after the dried, the chamazulene that can contain 100-200mg%, (-) of 200-450mg%-α-bisabolol, other opopanax body that only contains a small amount of 5-50mg%, numerical value is based on dry (that is: based on the absolute dry weight of spending).This absolute dry weight is to determine like this: in the drying baker, the single sample of Flos Chrysanthemi is placed on 105 ℃ of dryings down, until its constant weight (72-96 hour).At the activity substance content of 35-50 ℃ of temperature range inner drying flower, be on the dry weight based on the flowers of determining under being 105 ℃ in temperature.
If the Flos Matricariae chamomillae head inflorescence is gathered in the crops at growing period, can obtain especially effectively preparation (Flos Matricariae chamomillae) and these two kinds of content of active substance maximums of (-)-α-bisabolol), trophophase is meant when the tubular flower that 30-70% is arranged on the head inflorescence is open, 40-60% better, that is to say, be generally 50%, dry temperature is no more than 50 ℃, can be between 35-50 ℃, and 40 ℃ are better.For example: be exsiccant flower (flower has 40-60% tubular flower results in open period on fresh idea) under 40 ℃ the condition at dry the temperature inside the box, at least contain 100mg%, it also may be the chamazulene of 100-150mg%, at least contain 200mg%, also may be (-)-α-bisabolol of 200-300mg%, only contain other opopanax body of a spot of 5-50mg%.Numerical value is based on dry (that is: based on the absolute dry weight of spending).This absolute dry weight is determined by a kind of like this method: single only Flos Matricariae chamomillae sample is placed in the drying baker, and 105 ℃ dry down, until its constant weight (time is between 72-96 hour).The activity substance content of the flower of handling at 35-50 ℃ of temperature range inner drying is based on being on the dry weight of 105 ℃ of flowers of determining down in temperature.
The dried of Flos Matricariae chamomillae can be undertaken by air manually is provided, and also can dry in the shade, even can dry in the sun, and will guarantee heat but dry importantly under the sun, is no more than the required degree of bone dry.Should guarantee constant weight by Weight control in accordance with regulations.Dried can adopt the mode of nature, also can adopt the mode (as: adopting the warm air mode to carry out) of artificial treatment.Under the situation of no solar irradiation, carry out natural drying and handle, active matter quality is the highest.This moment, temperature should be between 40-60 ℃, and 40-50 ℃ better.Should carry out dried as early as possible after the results, preferably carry out at once.Every layer is difficult for too thickly during dried, and thickness can be between 5-20cm, and 10cm is better.Also can carry out dried in breezy shed, air themperature is between 20-30 ℃.Air themperature during dried generally should not be higher than 60 ℃.35-50 ℃ comparatively desirable.
According to this invention, the active matter quality of agent, especially chamazulene and these two kinds of main active substances content of (-)-α-bisabolol, the growth stage that flower is reached when depending on results also depends on the dried situation that spend the results back.The dried temperature is higher, and the degree of decomposition of active substance is just bigger, that is: active matter quality is just low more in the dry flower.Same reason, in the dried process, directly rayed has disadvantageous effect, should avoid as far as possible.
The trophophase of spending can recently be determined according to the open percentage of the tubular flower on certain time (this paper middle finger harvest time) each head inflorescence.Like this, the tubular flower opening reaches following percentage ratio on head inflorescence, as 30-50%, 30-70%, 40-60%, 60-100%, during 90-100%, all can gather in the crops flower, according to the Flos Matricariae chamomillae of this invention use, the particular growth phase that its flower reached, can determine its activity substance content, when all tubiflorous 40-60% were open, activity substance content was the highest, when open tubular flower was below or above this percentage ratio, activity substance content was all lower.Therefore, preferably this each individual plant of Flos Matricariae chamomillae should stop to bloom simultaneously, that is to say, the seed overwhelming majority that major part is sowed is emerged at one time, plant is in the identical florescence, as: on its head inflorescence of most plant, there is 40-60% open simultaneously in all tubular flowers.
Therefore, at whole flowers of can gathering of the most in good time phase; For this reason, the Flos Matricariae chamomillae that is used to produce preparation of the present invention also is particularly suitable for mechanical harvesting.
The dependency of inflorescence between residing time when following table has illustrated chamazulene and (-)-α-bisabolol content and results, and they and reclaim preparation of the present invention the dependency (each occasion, other opopanax body burden is no more than 50%) between the baking temperature commonly used.
The trophophase of flower during the baking temperature results
Have on 30 each head inflorescence on (air themperature) each head inflorescence and have 70
Open～100% tubular flower of～70% tubular flower is open
Active substance in the active substance dry products in the dry products
Content content
Be not higher than 50 ℃ of chamazulenes: 100mg% chamazulene at least: 40mg% at least
(-)-α-opopanax (-)-α-opopanax
Alcohol: 200mg% alcohol at least: 120mg% at least
Active substance in the active substance alcoholic extract in the alcoholic extract
Content content
Be not higher than 50 ℃ of chamazulenes: 5.0mg% chamazulene at least: 2.0mg% at least
(-)-α-opopanax (-)-α-opopanax
Alcohol: 10.0mg% alcohol at least: 6.0mg% at least
Active substance in the active substance dry products in the dry products
Content content
50～70 ℃ of chamazulenes: 50mg% chamazulene at least: 30mg% at least
(-)-α-opopanax (-)-α-opopanax
Alcohol: 150mg% alcohol at least: 100mg% at least
Active substance in the active substance alcoholic extract in the alcoholic extract
Content content
50～70 ℃ of chamazulenes: 2.5mg% chamazulene at least: 1.5mg% at least
(-)-α-opopanax (-)-α-opopanax
Alcohol: 7.5mg% alcohol at least: 10.0mg% at least
As can be seen from the table, if be used for producing the Flos Matricariae chamomillae that meets this invention preparation, its flower is results when 30～100% tubular flower is open on cephaloid, and reach 70 ℃ to gathering in the crops the back air themperature of flower when carrying out dried, so just can obtain so exsiccant material: its chamazulene content is 30mg% at least, (-)-α-bisabolol content always is at least 100mg%, and other opopanax body burden then always is lower than 50mg%.
In alcoholic extract, other opopanax body burden always is lower than 10mg%.
By the listed condition of last table with in the quintessence oil for preparing in the dry, chamazulene content at least always 3.5%, (-)-α-bisabolol at least always 10%, other opopanax body burden always is lower than 10%.
Be used for producing the Flos Matricariae chamomillae that meets this invention preparation, harvest time in evening in having, consistent production height, the compactness of blooming, head inflorescence is big, and this plant is particularly suitable for using mechanical harvest.In addition, at one time in the new tetraploid Flos Matricariae chamomillae seed of sowing, its plant is generally determined to stop at one time blooming, this also simplifies to a great extent and helps results.And this new Flos Matricariae chamomillae output height.This preparation for preparing from the Flos Matricariae chamomillae that meets this invention, be rich in bisabolol and female medicine azulene has anti-inflammatory activity, and it is used in diet, cosmetics and the Folium Camelliae sinensis industry.Therefore, in the prepared dry matter of defined terms in this invention, can obtain the Flos Matricariae chamomillae abstract.The proposition method can be passed through with ethanol or aqueous alcohol mixture, also can be by extracting with supercritical gas.Fresh Flos Chrysanthemi or freezing Flos Chrysanthemi provide and freeze flower, also can be used to prepare abstract.Especially alcoholic extract.In addition, Elecampane oil, (-)-α-bisabolol, chamazulene, and other Flos Matricariae chamomillae composition also can obtain from dry matter.
The alcoholic extract that meets the dry matter of " claim 1 " like this in ethanol spice extract, contains the chamazulene of 1.5mg% at least, and it is better to contain 5.0mg%; At least contain (-)-α-bisabolol of 5.0mg%, it is better to contain 10.0mg%.Other opopanax body burden should be lower than 8mg%.This class abstract prepares with usual way.
Can come to use mixing machinery in the leaching process, as: trough type mixing machine, percolation filter, or other suitable instrument for extracting, the main temperature range of using in the leaching process should be between 10～50 ℃.Needn't cool off.
Suitable solvent is straight chain aliphatic or side chain aliphatic particularly, monohydroxy or polyhydroxy-alcohol, and they contain 1-6 carbon atom; Belong to these as methanol, ethanol, 2-propanol, butanols, glycerol, a class material of isopropylidene glycerol; Also can adopt the mixture of these solvents and water.
Also can use the mixture of these solvents.Minimum quantity of solvent is 2: 1 with the ratio of dry matter.In general, should put 2～20 parts solvent in 1 part of dry matter.And it is better in 1 part of dry matter if put 3～10 parts solvent.
The quintessence oil that is obtained from the dry matter that meets " claim 1 " contains 3.5% chamazulene at least, and 5% is better; At least contain (-)-α-bisabolol of 10%, 15% is better; The content of other opopanax body should be lower than 10%.
The preparation quintessence oil can be placed on dry matter and be heated to boiling in the water, and ascorbic acid (as: as salt especially sodium salt) will be arranged in the water.PH value: 4-6 is if 5-5.5 is better.PH value is adjusted in the acid of available hydrochloric acid one class.For example: calculate by weight, the ascorbic acid (if putting) of the water of 10-50 part and 0.1-1 part can be used to boil 1 part dry matter.Need boil and boil 2～8 hours.
Rock with low aliphatic liquid hydrocarbon (as: petroleum ether (Bp35-60 ℃), pentane, naphthalene (support) dimethyl, naphthalane), the water distillation that is obtained is extracted repeatedly.With water drying (as: employing sodium sulfate), removing organic solvent carefully (as: can be undertaken by distillation in rotary evaporator, or preferably distill under 50～60 ℃ the temperature at 40～70 ℃), if utilize high boiling solvent to distill in a vacuum.
Use conventional spectrophotography, measure the chamazulene in the Flos Matricariae chamomillae abstract.
Use the conventional gas chromatography, measure (-)-α-bisabolol and other composition in the Matricariae aetheroleum.
Adnexa A has done detailed introduction to analysis determining method.
The active substance chamazulene is not to appear in the middle of the Flos Chrysanthemi with its original form, but exist with the form of Sesquiterpene matricin.Matricin shows with the similar pharmacological action of chamazulene.Chamazulene can form at once from the incipient stage of matricin, as: in when heating (as: steam distillation or at preserved material time).Therefore, the standard operation of Flos Matricariae chamomillae is not pointed out the content of matricin, and be meant the content of the chamazulene that forms by matricin.
If the flower of described tetraploid Flos Matricariae chamomillae, on head inflorescence, there is the tubular flower standard-sized sheet of 30-100% to put this growing period results, air themperature during simultaneously to these dried of spending reaches 70 ℃, so such preparation that obtains, (-)-α-bisabolol that can contain chamazulene and the 100mg% of 30mg% at least contains other opopanax body that is lower than 50mg%.By the quintessence oil of moisture distillation preparation, contain 3.5% chamazulene and (-)-α-bisabolol of 10% at least in such agent, contain other opopanax body that is lower than 10mg%.Simultaneously,, contain (-)-α-bisabolol of chamazulene and the 5.0mg% of 1.5mg% at least, contain other opopanax body that is lower than 10mg% with the pure abstract of lower alcohol extraction.
If the flower of described tetraploid Flos Matricariae chamomillae, on each head inflorescence, there is 30～70% tubular flower standard-sized sheet to put this growing period results, simultaneously, air themperature during to these dried of spending is not higher than 50 ℃, so such preparation that obtains, as for (-)-α-bisabolol of chamazulene that can contain 100mg% and 200mg%, contain other opopanax body that is lower than 50mg%.The quintessence oil of preparation in any way in this dose contains 3.5% chamazulene and (-)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%.Alcohol extract simultaneously contains (-)-α-bisabolol of chamazulene and the 10.0mg% of 5.0mg% at least, contains other opopanax body that is lower than 10.0mg%.
If the flower of described tetraploid Flos Matricariae chamomillae, all open these growing period results of tubular flower of 30～70% are arranged on an annual head inflorescence, air themperature is in 50～70 ℃ of scopes during simultaneously to these dried of spending, so, the preparation of Huo Deing like this, (-)-α-bisabolol that can contain chamazulene and the 150mg% of 50mg% at least contains other opopanax body that is lower than 50mg%.Any quintessence oil of preparation from this agent contains 3.5% chamazulene and (-)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%.Pure abstract contains the Flos Matricariae chamomillae of 2.5mg% and (-) of 7.5mg%-α-bisabolol at least simultaneously, contains other opopanax body that is lower than 10.0mg%.
If flower is that all open these growing period results of tubular flower of 70～100% are arranged on each head inflorescence, air themperature is not higher than 50 ℃ when simultaneously these flowers being carried out dried, so, the preparation of Huo Deing like this, (-)-α-bisabolol that can contain chamazulene and the 120mg% of 40mg% at least contains other opopanax body that is lower than 50mg%.The quintessence oil of preparation in any way in this dose contains 3.5% chamazulene and (-)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%.Simultaneously, pure abstract (-)-α-bisabolol of containing chamazulene and the 6.0mg% of 2.0mg% at least contains other opopanax body that is lower than 10.0mg%.Simultaneously, pure abstract contains (-)-α-bisabolol of chamazulene and the 6.0mg% of 2.0mg% at least, contains other opopanax body that is lower than 10.0mg%.
If the flower of described tetraploid Flos Matricariae chamomillae, all open these growing period results of tubular flower of 70～100% are arranged on each head inflorescence, air themperature during simultaneously to these dried of spending is between 50-70 ℃, the preparation of Huo Deing like this, at least (-)-α-bisabolol that contains chamazulene and the 100mg% of 30mg% contains other opopanax body that is lower than 50mg%.The quintessence oil of preparation arbitrarily in this agent contains 3.5% chamazulene and (-)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%.The alcohol abstract contains (-)-α-bisabolol of chamazulene and the 10mg% of 1.5mg% at least, contains other opopanax body that is lower than 10.0mg%.
Example 1
From the beginning material is the preparation that reclaims anti-inflammatory activity the tetraploid Flos Matricariae chamomillae:
Can broadcast in the ground in common mode obtaining Flos Matricariae chamomillae by described the sort of mode above.When plant grows, head inflorescence is in trophophase, and at this moment about 50% tubular flower is open, and available Flos Matricariae chamomillae picking machine is gathered in the crops these head inflorescences.Adjust the comb distance of picking machine, make the machine comb only open 50% head inflorescence to those tubular flowers and pluck.Before further handling, should transport to a shady and cool place to the flower after the results as early as possible, scatter thinly.Then, head inflorescence is placed in the sieve to remove cane and to be dried to constant weight.The loss in weight about 80%.Can be in the cool, flower is placed on carries out dried on the shelf, bed thickness is about 10cm, and dry place ventilates and will get well.
Can finish dried after 2 to 3 days.Exsiccant material is sieved, and to remove the chip of cane fragment and flower, it is bundled then to pack.
Analyze
Quintessence oil: 960mg%
Chamazulene: 162mg%
(-)-α-bisabolol: 330mg%
If carry out dried on fixed belt drying device, and the air themperature of artificial heating is between 50～70 ℃, and dry run needs 4-5 hour so.Exsiccant material is sieved, and to remove the chip of cane fragment and flower, it is bundled then to pack.
The preparation of Huo Deing in this way, its analytical data is as follows:
Quintessence oil: 870mg%
Chamazulene: 94mg%
(-)-α-bisabolol: 197mg%
Used tetraploid Flos Matricariae chamomillae beginning material obtains by following method:
By tetraploid Flos Matricariae chamomillae kind (seeing " Herba Hungar " the 4th phase (1) 125-169 page or leaf (1965)) the 10000 strain individual plantses of being described by I.Sarkany being carried out the variability test, found that about per 1, in 000 individual plant, just there is strain chamazulene content in its quintessence oil to calculate by weight) up to 20%(; Calculate by weight up to 50%(at (-)-α-bisabolol content), the content then very low (calculate by weight, be lower than 5%) of other opopanax body (particularly (-)-α-opopanax oxidation of ketones thing).These individual plants are elected, are followed these steps to do:
Step 1:
In the filial generation of selected tetraploid Flos Matricariae chamomillae (as mentioned above), selected the individual plant that meets following condition:
A) bloom in about same time
B) its part branch is even, flower portion compactness, about 10cm, preferably 5cm.
C) head inflorescence is big, the about 30mm of external diameter, and 25～35mm is better.
D) meet or exceed minimum chamazulene content 150mg%, this standard of bisabolol content 300mg%.Other opopanax body (especially bisabolol oxide) will be lower than all numerical value of 50mg%(significantly all based at 40 ℃ of dry capitulums; During results, all tubiflorous 0-70% are open on every strain head inflorescence).
Clone to these plants is handled, in order to reach this purpose, earlier plant (wanting the clone maternal plant) is pruned weak point, make its branch be about 15cm, then being exposed to the every day irradiation is 8-10 hour, under the temperature 12-14 ℃ of condition so that short its regrows (short lateral bud).To lack lateral bud and cut, implant in the mixture of peat and sand.Be about 100% at relative air humidity, air themperature is 15 ℃, 14 hours sun exposure, and under such condition, taking root of cutting needs to continue 7-14 days.
Except that above-mentioned traditional cloneization (cottage propagation), but also the isolated culture of the meristem of available plant is bred (that is: so-called separate living tissue breeding).The various piece of Flos Matricariae chamomillae plant all is suitable for breeding, but better with stem apex or axillalry bud.
Plant is used H
2O
2After washing, the superclean bench under aseptic condition takes out stem apex from the axil bud; It is received on the murashige and Skoog culture medium in the test tube (seeing " Physiol, Plant " 15 phases, 473-497 page or leaf, 1962).Then test tube is placed in the growth cabinet, accepting the every day light application time is 12～18 hours, is (fluorescent light), light intensity: 500-10 well with 16 hours, and 000lux is better with 1000-3000lux.Temperature is 15～30 ℃; With 22～27 ℃ better.
When explant grows fine, it is changed on the above-mentioned culture medium, but wherein contain the cytokinin concentration of higher concentration
*(as: the 6-isopentennyladenine that 30mg/ rises), and a spot of auxin (heteroauxing that 0-0.3mg/ rises).Like this, axillalry bud has just grown tall, and produces indefinite organ and more axillalry bud.Then, they can be taken out and cultivate again by mentioned above.
For a large amount of breedings, can be with explant through three successive transfer culture or the third generation, when its after growth on above-mentioned first culture medium and forming blade, be transferred to and contain 10mg/ and rises heteroauxing or indolebutyric acid, or on the culture medium of the α-Nai Yisuan of 0.1-0.3mg/ liter.Plantlet is taken root, and approximately through 4 week, it is planted in the flowerpot that sterilization garden soil (120 ° steam treated 12 hours) is housed, and puts into greenhouse cultivation (condition is bred with normal cutting propagation) later on again.
*The basic element of cell division is to promote fissional phytohormone.
Murashige and Skoog culture medium:
Mg/ rises the mg/ liter
NH
4NO
3400 heteroauxings 2.0
Ca(NO
3)
24H
2O 144 kinetins 0.1
KNO
380 thiamines 0.1
KH
2PO
412.5 nicotinic acid 0.5
MgSO
47H
2O 72 pyridoxins 0.5
KCl 65 glycine 2.0
NaFe-EDTA 25 inositols 100
H
3BO
31.6 casein hydrolysis 1000
MnSO
44H
2O 6.5 sucrose 2%
ZnSO
47H
2O 2.7 pure agar powders 1%
KI 0.75
Step 2:
Plant by " step 1 " gained under the condition of separating in the greenhouse, stops to bloom simultaneously.These plants are to be placed on size in the flowerpot of 11cm, and the soil of cultivation garden soil, day temperature are 18～24 ℃, and night, temperature was 12～14 ℃.Have 14 hours irradiation every day at least, replenish (200 watts/meter with light in the winter time
2).Supply water as required.
In all individual plant of selecting, the withered head inflorescence that maybe will disintegrate is gathered in the crops, to reclaim seed, harvest time continued for 4 weeks.Carry out dried in breezy shed, air themperature is between 20 °～30 ℃.(sieve in 5 * 0.4mm), reuse lifting separator purifies seed in slotted screen seed then.
Step 3:
Take out the seed sample that is obtained in " step 2 " lining randomly, therefrom obtain about 2000 plant (ecological condition is the same with " step 2 "), (A) to (D) standard to its " step 1 " lining is selected, and will select individual plant and be for further processing by " step 2 ".
Step 4:
Some seeds that obtained in " step 3 " in autumn kind different local at two.
The area I):
450m MSL，48.5° N/11.5°E
Total rainfall per year is 750mm.
The weather of moistening gentleness,
January-10-0 ℃
July+10-20 ℃
The area II):
200m MSL，42° N/1°E，
Total rainfall per year is 400mm
Etesian climate,
0-+10 ℃ of January
July+20-+30 ℃
The MSL=mean sea level, the north=sea level
° N=north latitude (the no number of degrees)
° E=east longitude (the no number of degrees)
In two areas, all be to sow at JIUYUE end or the beginning of October.
Growing crop is thus carried out field experiment, check and measure the concordance of the growth of (having classified), the size of flower and harvest time thereof.In addition, randomization has been analyzed activity substance content in the flower.The individual plant of those parameters of mentioning meeting in " step 1 " chooses once more.According to final stage in " step 2 ", from these individual plants, reclaim seed.
Step 5:
Step 4 gained seed again with same order, is repeated to do step 3 and 4.
Step 6:
From in that " seed that obtains the step 5 (its ecological condition as " step 2 " as described in) lining has obtained about 1500 individual plants and has selected by the same standard of " step 1 ".From wherein selecting 34 individual plants again, obtain their clones by " step 1 ", 10 strains in each clone are planted in the open air; Random alignment, separately sowing, spacing be 40 * 30cm(in regional I referring to " step 4 ") soil is yellow soil, PH is 7.0, the time of planting is at the beginning of June.Gather in the crops first seed mid-July.Afterwards, prune plant, it is rebloomed.At the bottom of mid-August to August, gather in the crops second batch of seed.
According to " step 2 ", this material has been carried out the seed recovery.
The seed that is obtained in " step 6 " is exactly by meeting the Flos Matricariae chamomillae breeding seed that process obtained of this invention regulation.
The flower of the plant that cultivates from these seeds (sowing of JIUYUE or October, next year the beginning of June results) should be plucked when the tubular flower of 30-70% is open, then dried (in drying baker, the time is 72 hours, and temperature is 40 ℃) immediately; Such flower can contain the chamazulene of 150mg%, (-) of 300mg%-α-bisabolol at least, contains other opopanax body that is at most 50mg%, and above parameter all calculates by the weight of dried floral (that is: dry matter).
Example 2
From the beginning material is to reclaim the new agent with anti-inflammatory activity the tetraploid Flos Matricariae chamomillae:
By the Flos Matricariae chamomillae of hereinafter being introduced that mode obtained, down on the farm with the general fashion kind.When plant grows, when head inflorescence had reached open this growth stage of about 50% tubular flower, available Chamomile picking machine was gathered in the crops these head inflorescences.Adjust the comb distance of picking machine, make the machine comb only open 50% head inflorescence to those tubular flowers and take off.Before doing further processing, should transport to a shady and cool place as soon as possible the flower after the results, scatter thinly.Then, in diagrid, remove cane head inflorescence is dried to constant weight.The loss in weight about 80%.Can be in the cool, flower is placed on carries out dried on the shelf.The about 10cm of bed thickness, dry place ventilates and will get well.
2～3 days can finish dried.Exsiccant material is sieved, remove the chip of stem fragment and flower, it is bundled then to pack.
Analyze
Quintessence oil: 940mg%
Chamazulene: 132mg%
(-)-α-bisabolol: 243mg%
If dried is carried out on fixed belt drying device, the air themperature of artificial heating will be between 50～70 ℃, and dry run needs 4-5 hour.Exsiccant material is sieved, remove the chip of stem fragment and flower, then being bundled into bundle.
The analytical data of the agent of Huo Deing is as follows in this way:
Quintessence oil: 775mg%
Chamazulene: 65mg%
(-)-α-bisabolol: 163mg%
Used tetraploid chrysanthemum beginning material obtains in the following manner:
To tetraploid Flos Matricariae chamomillae kind (seeing " Herba Hungar " 1965 4(1) P125-569 that describes by I.Sarkany) about 10, the test of individual plant variability is carried out in 000 strain, the strain chamazulene content in its quintessence oil that just has in per 100 individual plants that found that is calculated by weight up to 20%(), in its quintessence oil, (-)-α-bisabolol content is up to 50%, content is then very low (calculates by weight for other opopanax body (particularly (-)-α-opopanax oxidation of ketones thing), be lower than 5%), these individual plants are elected, are followed these steps to do:
Step 1:
In the tetraploid Flos Matricariae chamomillae progeny plant of electing in a manner described, select such individual plant strain: its minimum chamazulene content reaches or surpasses 100mg%, the minimum content of (-)-α-bisabolol meets or exceeds 200mg%, and other opopanax body burden then is lower than especially bisabolol oxide of 50mg%().These numerical value calculate according to following condition: temperature was about 40 ℃ when head inflorescence was dry; Locate such stage during results, all tubular flowers have 30～70% opening approximately on each head inflorescence.
Step 2:
On the plant that from " step 1 ", is obtained, take all open fresh ideas.These plant are put into the greenhouse, under the condition of separating, stop together blooming.These plant are put into size to be planted for the flowerpot of 11cm is foster.Flowerpot should be piled garden soil.Day temperature is 18～24 ℃, night: 12～14 ℃.Has 14 hours irradiation every day at least.If in the winter time, assist to reach (200 watts/meter with light
2).Water is provided as required.
In all individual plants of selecting, the head inflorescence that maybe will disintegrate of will withering is gathered in the crops, to reclaim seed.Harvest time continued for 4 week.Carry out dried in breezy shed, air themperature is between 20～30 ℃, and (sieve in 5 * 0.4mm), reuse lifting separator purifies seed at the flute profile sieve seed then.
The seed that is obtained in " step 2 " is exactly by meeting the Flos Matricariae chamomillae breeding seed that process obtained of this invention regulation.The flower of the plant that plants from these seeds (sowing of JIUYUE or October, next year the beginning of June results), when 30～70% tubular flowers are open, pluck, dried (in drying baker, carry out the time: 72 hours, temperature: 40 ℃) immediately then; Such flower can contain (-)-α-bisabolol of chamazulene and the 200mg% of 100mg% at least, contains other opopanax body that is no more than 50mg%.Above parameter is all calculated by the weight of dried floral.
Example 3
From parent material is to reclaim the preparation with anti-inflammatory activity the diploid Flos Matricariae chamomillae:
By the following Flos Matricariae chamomillae of introducing that mode obtained, with the conventional method kind in the field.
When plant grows, when head inflorescence had reached open this growth stage of about 50% tubular flower, available Flos Matricariae chamomillae picking machine was gathered in the crops these head inflorescences.Adjust the comb distance of picking machine, make the machine comb only open 50% flower to those tubular flowers and pluck and adopt.Before doing further processing, should transport to a shady and cool place to the flower after the results as early as possible, scatter thinly.Then in diagrid, remove cane, head inflorescence is dried to constant weight.The loss in weight about 80%.Can in the cool flower be placed on the shelf, carry out dried.Bed thickness is about 10cm, and dry place ventilates and will get well.
Can finish dried after 2 to 3 days.Remove the fragment and the flower chip of stem, it is bundled then dry matter to be packed.
Analyze
Quintessence oil: 910mg%
Flos Matricariae chamomillae: 117mg%
(-)-α-bisabolol: 252mg%
Used diploid chrysanthemum beginning material following manner obtains:
To Franz, Ch., J.Hoelzl, the described rudimentary bisabolol of A.Voemel, but do not have bisabolol and diploid Flos Matricariae chamomillae and (see " Acta Horti-Culturae ", 1978,73 phases, the 109-144 page or leaf) the individual plant variability test of carrying out, found that in all individual plants, nearly 20% individual plant contains 20% chamazulene (calculating by weight) approximately in its quintessence oil, and (or) containing (-)-α-bisabolol (calculating by weight) up to 50%, their other opopanax body burden is very low.Select these individual plants, then it doubled by following method:
With the Flos Matricariae chamomillae seed of electing like this, be placed on the filter paper of the 0.05% moisture colchicine solution that infiltrated, it was expanded 6 hours, indoor temperature is 20 ℃.Then it is taken down from filter paper, water washes repeatedly.It is planted in the seed pan (places in the greenhouse).Soil adopts charcoal mud-Sha mixed soil, and ratio is 1: 1.Temperature: 18-20 ℃, relative air humidity: about 60%.Illumination every day 14 hours is subsidized with artificial lighting.
Observe the plant that is germinateing, observe the flowering phase always.By comparing and measuring the size of pollen and seed, reach the result who measures polyploidization by the meter Chromosome number.(F
1The first generation filial generation of-filial generation=) from handling with colchicine, assert that tetraploid plant seed development was come out.
Can double to handle by following method:
Get and physically well develop 5-7 days Seedling chrysanthemum Seedlings order, that on the filter paper that has soaked water, germinate.Be placed in 0.05% colchicine solution, solution should not have cotyledon, and the time is 4-6 hour, and indoor temperature is 20 ℃.The processing of being careful of very thin seminal root.Dried bad in order to prevent, ambient atmosphere should have 100% relative air humidity.After the processing, the seedling water is washed repeatedly, be transplanted in the cultivation case.With the same quadrat method of handling seed, further handle these seedling.With thunder thatch (Leitz) binocular research microscope, the mirror of use micrometer eyepiece and micrometer is measured the size of female pollen.
Measure Chromosome number with the tip of a root:
The plantlet of cultivating from the greenhouse or broadcast in the transplanting strain is got fresh long end of its 1-2cm, puts into 0.002 molar oxyquinoline solution, soaks 5 hours; Soaked 15 minutes at the HCl that puts into 1N afterwards.During check, get the tip of a root of about 1 millimeters long,, then immerse and carry out microscopic in the oil with 2% orcein-acetic acid dyeing.If cell is in the mitosis state, it is 4 times (4 just can determine somatic chromosome set
n=36).
Such plant approximately is about 30 greater than the 50%(of diploid parent material as its pollen diameter, rather than 20 μ m); Its somatic chromosome group is two times that diploid sends out, that is: the corresponding number of 36(liploid plant is 18), they are exactly that tetraploid is selected these plant.The seed of nearly 0.1-0.5%, seedling can carry out tetraploidization by the way more precisely, and develops into the complete plant strain that can bloom.The plant of these acquisitions then can come it is handled by the step 1 and 2 in " example 2 ".
The Flos Chrysanthemi of Huo Deing can contain the chamazulene of 100mg%, (-) of 200mg%-α-bisabolol at least like this, (results are that the tubular flower of 30-70% is open period to contain other opopanax body of being no more than 50mg%, carry out dried with drying baker, the time is 72 hours, and temperature is 40 ℃).
Implement 4
From parent material is to reclaim the preparation with anti-inflammatory activity the diploid Flos Matricariae chamomillae:
By the Flos Matricariae chamomillae of introducing below that mode obtained, with the conventional method kind in the field.
When plant grows, head inflorescence has reached when open this growth stage of 50% tubular flower is arranged approximately, and available Flos Matricariae chamomillae picking machine is gathered in the crops these head inflorescences.Adjust the comb distance of picking machine, make the machine comb only open 50% flower to those tubular flowers and pluck and adopt.Before doing further processing, should transport to a shady and cool place to the flower after the results as early as possible, scatter thinly.Then, in diagrid, remove cane, head inflorescence is dried to constant weight.The loss in weight about 80%.Can be in the cool, flower is placed on the shelf handles.Bed thickness is about 10cm.Dry place ventilates and will get well.
After 2～3 days, can finish dried.Dry matter is sieved, remove the fragment of stem and the chip of flower, it is bundled then to pack.
Analyze
Quintessence oil: 1020mg%
Chamazulene: 173mg%
(-)-α-bisabolol: 418mg%
Used diploid sends out chrysanthemum beginning material and obtains in the following manner:
To by Franz, Ch., J.Hoelzl, the rudimentary bisabolol that A.Voemel introduces, (do not see " Acta and there is bisabolol diploid Flos Matricariae chamomillae, Horti-Culturae " the 73rd phase, the 109th～114 page (1978) carry out the test of individual plant variability, found that in all individual plants, nearly 20% individual plant in its quintessence oil, contain approximately 20% chamazulene (calculating by weight) and (or) contain (-)-α-bisabolol (calculating by weight) up to 50%, the content of other opopanax body (especially (-)-α-opopanax oxidation of ketones thing) is very low.Select such individual plant, then, make its tetraploidization fully by method in " example 3 " lining.Afterwards, select the individual plant of tetraploidization.
Nearly 0.1～0.5% seed, seedling can be by tetraploidization more precisely, and develops into plant complete, that can bloom.The plant of Huo Deing like this can be cooked to 6 by the step 1 in the example 1.
The Flos Chrysanthemi of Huo Deing like this can contain the chamazulene of 150mg%, (-) of 300mg%-α-bisabolol at least; Contain other opopanax body of 50mg% at the most (results periods is to have the tubular flower of 30-70% open, carries out dried with drying baker, and the dried time is 72 hours, and temperature is 40 ℃).
Other characteristic is the same with the characteristic of the Flos Matricariae chamomillae of being obtained by example 1.
Example 5
The example of preparation matricariano abstract:
Get the dry matter 400g that meets " example 1 ", (calculate by weight: 40% ethanol), dry matter was extracted 3 hours in the slot type blender, mixer speed is 30 rev/mins with 2100 gram aquiferous ethanol.Then push this material, and abstract is filtered.Abstract contains the chamazulene of 3.7mg%, (-) of 10.2mg%-α-bisabolol.
Used Flos Chrysanthemi, results are to have the tubular flower of 30-70% open on the head inflorescence period.Temperature must not be higher than 50 ℃ when dry, obtains dry matter like this, contains chamazulene 105mg%, contains (-)-α-bisabolol 12mg%.
Example 6
Preparation quintessence oil example:
Get one 5 liters round-bottomed flask, put into 3.6 premium on currency, the sodium ascorbate of 2 grams, the dry matter (dried is to carry out under the condition of no sunlight, and temperature is 50 ℃) in 200 grams " example 1 "; HCl with 1N adjusts to 5.0 with pH value.After adding zeolite, the material in the bottle is heated to boiling temperature, after about 3 hours, just can collect about 1.2 liters distillation.
When distillation was finished, the petroleum ether with 100ml rocked 3 times with the extractive distillation thing.Then distillation is carried out dried above anhydrous sodium sulfate.Anhydrous solution is filtered; Then with rotary evaporator, solvent is distilled.Quintessence oil output is 1.44 grams, wherein contains 3.6% chamazulene, 10.2% (-)-α-bisabolol.
Adnexa A
Reclaim quintessence oil
Parent material is the Flos Matricariae chamomillae dry, and the Flos Matricariae chamomillae type is to produce antiinflammatory to provide according to the present invention.Only adopt that those have 30-70%, preferably the open head inflorescence of 40-60% tubular flower prepares quintessence oil.Carry out dried in drying baker, the time is 72 hours, and temperature is 40 ℃.
Adopt the method for hereinafter being introduced, dry matter is carried out steam distillation, to reclaim the quintessence oil of Flos Chrysanthemi:
In the round-bottomed bottle of some liters, amplify the dried floral of the no fine debris of 2.0 grams, add the deionized water of 250ml again.Then to the dried floral of these no fine debris, (in order to a small amount of quintessence oil is carried out the steam reflux of quantitative assay) carries out cohobation in a Ke Laiwen device, and the time is 2 hours.Each pentane of 1 milliliter of employing of analyzing is as receiver media.Reflux rate is 40 ± 4 droplets/minute.After distillation is finished, will be dissolved in quintessence oil in the pentane and dewater fully and pour in the test tube, and with residual quintessence oil in the pentane decontaminating apparatus.In order to remove the water that left behind, can in solvent, add the dry Na of a spatula point
2SO
4; Then, by a glass nutsch filter, its filter cell size is D
3Or D
4, filtrate solvent is gone in the flask on limit, garden.In 40 ℃ water-bath, boil off pentane, and dry in exsiccator, measure the quintessence oil amount by measuring gravimetric method.
The chamazulene and the bisabolol content (quintessence oil of about 20mg) of the quintessence oil that subsequently, can determine by this way to be obtained.
The spectrophotometry chamazulene
Measure solution: all, by above-mentioned way doing from 2 grams
The quintessence oil that obtains in the dry material (approximately
20mg), be dissolved in the n-of 25ml
In ethane or the cyclohexane solution.
Gauge: filter photometer and (can adopt Eppendorf
Type).
Wavelength: 578nm
Cuvette (Cuvette): 1cm
The extinction ratio of Flos Matricariae chamomillae
（Ig/100ml;1cm）：20.8
Compensation liquid: n-hexane or cyclohexane extraction
Finding chamazulene content
（mg/100g）：120 E
578
If do not have filter plotometer when measuring, available spectrophotometer is measured.
Gauge: spectrophotometer (can be selected for use
Zeiss PM QⅡ
Type or PM Q III type)
Wavelength: 605nm
Cuvette (Cuvette): 1cm
The extinction ratio of Flos Matricariae chamomillae
（Ig/100ml;
1cm）：24.5
Compensation liquid: n-hexane or cyclohexane extraction
Finding chamazulene amount
（mg/100g）：102 E605
Subsequently, can in dosage solution, measure the content of bisabolol.
Gas Chromatographic Determination bisabolol and other opopanax body
Gas chromatograph: Hewlett Packard type
5750，Erba
Fractovap 2350 types or
Similar instrument.
Detector: flame ionisation detector
Carrier gas: helium
Post: 1/8 inch; 200cm: steel column
Column packing: 3% the cyanogen silicon of silanization on diatom goes out
Rubber " XE60 " " Chromosorb
WAW HP " 125 to
150 μ m are as carrier gas species.
Temperature:
Detector: 320 ℃
Injection site: 220 ℃
Column temperature: 85～220 ℃
Temperature program(me): 4 °/minute
Sample solution: adopt and measure chamazulene with measuring solution.
With reference to solution: in the cyclohexane solution of 25ml, should
The standard opopanax that divides the 1.5mg that terminates an agreement
Alcohol.
Injection volume: the sample solution of 5 μ l, with the ginseng of 5 μ l
According to solution.
Estimate: estimate by the reference peak value.
Every 100g drying: the 10 * part (reference) of weighing
Bisabolol in spending (mg) *
Content mg peak value (sample)
Peak value (object of reference)
Other opopanax body, also can under following condition, use gas Chromatographic Determination:
Instrument: Packard, 7721 types,
800 series, or Erba
Fractovap series 2350
Post: glass column, diameter 3m/2mm;
Diameter 2m/2mm
Column packing: 3% on kieselguhr the methylbenzene of silanization
Base (gathering) silicone rubber " OVI ";
" Gaochrom Q " 125 to
150 μ m are as carrier gas species
Carrier gas: 30ml/ divides N2
Temperature program(me): 80 to 180 ℃,
2.5(3) ℃/minute
Injection portion
Detector temperature: 200 ℃
Detector: flame ionisation detector
Injection volume: be about at 1: 50 o'clock in ratio, have approximately
The quintessence oil dilution of 2 μ l.
Part evaluation can be used interior mark, and a part can not used interior mark.The suitable interior mark that is used for thinking poorly of chamazulene or does not have a quintessence oil of chamazulene is the lauric acid methyl ether, or hexadecane.If the chamazulene content of quintessence oil is higher than 5%, so just adopt chamazulene to make interior mark, then measure content (wavelength 578nm) with photometric mode.
Claims (6)
1, from Flos Matricariae chamomillae, reclaims the method for agent having ahtiphlogistic activity, it is characterized in that selecting from the tetraploid Flos Matricariae chamomillae such plant: their flower is after dried under 40 ℃ of temperature, should contain the chamazulene of 100 milligrams of % and (one)-α-bisabolol of 200 milligrams of % at least, the content of other opopanax body then should be lower than 50 milligrams of %.The Main Ingredients and Appearance of quintessence oil should be measured (one)-α-bisabolol.The plant of Huo Deing in this way also can be through further selecting, after the propagation steps, be in growth stage at it, results flower when promptly having the tubular flower of 30-100% open on each head inflorescence.And it can not be surpassed under 70 ℃ the condition at maximum temperature carry out dried, to obtain a kind of dry matter.The chamazulene content of this dry matter is at least 30 milligrams of %, and (one)-α-bisabolol content is at least 100 milligrams of %, and other opopanax body burden should be lower than 50 milligrams of %.Then, at random adopt to contain hydrodistillation a kind of quintessence oil of preparation from the dry matter that obtains with said method.Quintessence oil contains 3.5% chamazulene, (one)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%, or at random adopts lower alcohol extraction, from the dry matter that obtains with said method, prepares a kind of pure Flos Matricariae chamomillae abstract.Abstract contains the chamazulene of 1.5 milligrams of %, (one)-α-bisabolol of 5.0 milligrams of % at least, contains other opopanax body that is lower than 10 milligrams of %.
2, method according to claim 1 is characterized in that the tetraploid Flos Matricariae chamomillae is to obtain from the diploid Flos Matricariae chamomillae that a kind of quintessence oil Main Ingredients and Appearance is (-)-α-bisabolol, method is by chemical drugs, and temperature is 0-35 ℃; Gamma-radiation, the X-ray, ultraviolet, temperature is 0-35 ℃; 33-50 ℃ high temperature and 0-5 ℃ K cryogenic treatment doubles the diploid Flos Matricariae chamomillae.
3, method according to claim 1 is characterized in that flower should be in its growing period results, and at this moment, the tubular flower of the 30-70% that has on each head inflorescence should be opened.Flower needs dried, and the air themperature during processing must not be higher than 50 ℃.The dry matter of Huo Deing in this way, should contain the chamazulene of 100 milligrams of %, (-)-α-bisabolol of 200 milligrams of % at least, contain other opopanax body that is lower than 50 milligrams of %, the quintessence oil that from this dry matter, at random prepares, at least contain 3.5% chamazulene, 10% (-)-α-bisabolol contains and is lower than other opopanax body of 10%; The pure abstract that from this dry matter, prepares, its chamazulene content is at least 5.0 milligrams of %, and (-)-α-bisabolol content is at least 10.0 milligrams of %, and other opopanax body burden then is lower than 10.0 milligrams of %.
4, method according to claim 1, it is characterized in that flower is in its growing period results, at this moment, there is the tubular flower of 30-70% to open in each head inflorescence, flower needs dried, the air themperature during processing between 50-70 ℃, the dry matter of Huo Deing like this, at least contain the chamazulene of 50 milligrams of %, (-)-α-bisabolol of 150 milligrams of %, contain other opopanax body that is lower than 50 milligrams of %.The quintessence oil that at random prepares from this dry matter contains 3.5% chamazulene, (-)-α-bisabolol of 10% at least, contains to be lower than other opopanax body of 10%; The pure abstract for preparing from this dry matter contains the chamazulene of 2.5 milligrams of %, (-)-α-bisabolol of 7.5 milligrams of % at least, contains other opopanax body that is lower than 10.0 milligrams of %.
5, method according to claim 1 is characterized in that flower in its growing period results, at this moment, has the tubular flower of 70-100% to open on each head inflorescence.Flower need carry out dried, temperature must not be higher than 50 ℃ during processing, the dry matter of Huo Deing like this, at least the chamazulene that contains 40 milligrams of %, 120 milligrams (-)-α-bisabolol, contain other opopanax body that is lower than 50 milligrams of %, the quintessence oil that from this dry matter, at random prepares, at least contain 3.5% chamazulene, (-) α-bisabolol of 10%, contain and be lower than other opopanax body of 10%, the pure abstract that from this dry matter, prepares, at least the chamazulene that contains 2.0 milligrams of %, 6.0 (-)-α-bisabolol of milligram % contains other opopanax body that is lower than 10.0 milligrams of %.
6, require one or more described methods according to aforesaid right, it is characterized in that flower in its growing period results, at this moment, has the tubular flower of 70-100% to open on each head inflorescence.Flower needs to carry out dried under air themperature is 50-70 ℃ condition, the dry matter of Huo Deing like this, at least contain 30 milligrams chamazulene, (-)-α-bisabolol of 100 milligrams of %, contain other opopanax body that is lower than 50 milligrams of %, the quintessence oil that from this dry matter, at random prepares, at least contain 3.5% chamazulene, (-)-α-bisabolol of 10%, contain and be lower than other opopanax body of 10%; The pure abstract for preparing from this dry matter contains the chamazulene of 1.5 milligrams of %, (-)-α-bisabolol of 10 milligrams of % at least, contains other opopanax body that is lower than 10.0 milligrams of %.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3446217 | 1984-12-19 | ||
| DE19843446217 DE3446217C2 (en) | 1984-12-19 | 1984-12-19 | Process for the preparation of a new antiphlogistic agent |
| DEP3446217.1 | 1984-12-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85108903A true CN85108903A (en) | 1986-06-10 |
| CN1034631C CN1034631C (en) | 1997-04-23 |
Family
ID=6253138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN85108903A Expired - Lifetime CN1034631C (en) | 1984-12-19 | 1985-12-18 | Process for producing a new agent having ahtiphlogistic activity |
Country Status (7)
| Country | Link |
|---|---|
| CN (1) | CN1034631C (en) |
| BG (1) | BG50925A3 (en) |
| DD (1) | DD240135B5 (en) |
| DE (1) | DE3446217C2 (en) |
| FI (1) | FI81007C (en) |
| PT (1) | PT81701B (en) |
| YU (1) | YU45993B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2402802C3 (en) * | 1974-01-22 | 1979-06-13 | Deutsche Gold- Und Silber-Scheideanstalt Vormals Roessler, 6000 Frankfurt | Obtaining a chamomile drug with a high content of chamazulene and bisabolol |
| DE3423207C3 (en) * | 1983-06-29 | 1996-09-26 | Asta Medica Ag | Process for producing a new type of chamomile (name Manzana) |
| DE3542756C3 (en) | 1984-12-19 | 1997-09-04 | Asta Medica Ag | Process for the preparation of a new tetraploid and bisabolol-rich chamomile with improved properties |
| CN1003201B (en) * | 1985-06-12 | 1989-02-01 | 三菱雷机株式会社 | Switching device for timing switch |
-
1984
- 1984-12-19 DE DE19843446217 patent/DE3446217C2/en not_active Expired - Lifetime
-
1985
- 1985-12-10 BG BG72675A patent/BG50925A3/en unknown
- 1985-12-17 YU YU197085A patent/YU45993B/en unknown
- 1985-12-17 DD DD28455085A patent/DD240135B5/en active IP Right Maintenance
- 1985-12-18 PT PT8170185A patent/PT81701B/en unknown
- 1985-12-18 FI FI855061A patent/FI81007C/en not_active IP Right Cessation
- 1985-12-18 CN CN85108903A patent/CN1034631C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE3446217A1 (en) | 1986-07-10 |
| FI81007C (en) | 1990-09-10 |
| FI855061A0 (en) | 1985-12-18 |
| CN1034631C (en) | 1997-04-23 |
| BG50925A3 (en) | 1992-12-30 |
| FI81007B (en) | 1990-05-31 |
| YU197085A (en) | 1988-06-30 |
| DD240135B5 (en) | 1994-04-21 |
| FI855061A (en) | 1986-06-20 |
| DE3446217C2 (en) | 1994-05-19 |
| PT81701B (en) | 1988-02-17 |
| PT81701A (en) | 1986-01-02 |
| YU45993B (en) | 1992-12-21 |
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