CN85106076B - pollen wall breaking technology - Google Patents
pollen wall breaking technology Download PDFInfo
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- CN85106076B CN85106076B CN85106076A CN85106076A CN85106076B CN 85106076 B CN85106076 B CN 85106076B CN 85106076 A CN85106076 A CN 85106076A CN 85106076 A CN85106076 A CN 85106076A CN 85106076 B CN85106076 B CN 85106076B
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- pollen
- water
- freezing
- wall
- pure
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- Cosmetics (AREA)
- Jellies, Jams, And Syrups (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The wall breaking technology of pollen, by pollen is soaked, freezing, decompression and homogenized destroy pollen wall, separate by centrifuge then, so that the pollens nutrition thing separates with pollen wall, make pure pollens nutrition thing Normal juice or make again the crystalline powder of pure pollens nutrition thing Normal juice through lyophilization.This Normal juice or crystalline powder are easy to be absorbed by human body, can be widely used in the fields such as food, daily cosmetics and medicine.
Description
The present invention is a kind of wall breaking technology about pollen, uses this technology can not contained the pure pollens nutrition material of pollen wall.
Pollen is the abundant material of a kind of natural, nutrition.These nutriments are present among the encirclement of pollen wall, therefore, fully absorb the nutrition of pollen.Just must destroy pollen wall.Before the present invention made, reported wall breaking technology had enzymatic isolation method and mechanical crushing method.Enzymatic isolation method is little to sporopollenin effect the firmest in the pollen wall, thereby broken wall efficient is lower, and simultaneously, enzymatic isolation method also might be brought mould in the product into; The operating temperature of mechanical crushing method is generally all higher, active material in the destructible pollen, thereby reduction nutritive value, and when mechanical crushing method is broken to pollen wall below 5 microns, just be difficult for the fragment of pollens nutrition material with wall separated, the fragment of these pollen walls causes that easily the human allergy reacts after mixing pollen product.
Task of the present invention provides a kind of new pollen wall breaking technology.The broken wall efficient height of this technology, its each link is physical process, and all carries out at a lower temperature, does not destroy active material in the pollen, and the pollens nutrition material is separated with pollen wall, makes pure pollens nutrition thing Normal juice or its crystalline powder.
Technical essential of the present invention is: pollen is soaked in water.Then through freezing, through reduced pressure treatment, at last by homogenizer homogenate, the weakness of pollen wall is destroyed again, form some breach, reach pollen broken wall and not broken wall, the pollens nutrition material just is released in the water by the breach on the pollen wall, through centrifugation, nutriment is separated with pollen wall, make pure pollens nutrition thing Normal juice, if necessary, can be made into again the crystalline powder of pure pollens nutrition material through lyophilization.
Further specify implementation step of the present invention below in conjunction with example:
The first step: dried Bee Pollen raw material is removed impurity.
Second step: the dried flower powder after the removal of impurities is ground to form single pollen grain.
The 3rd step: the ratio with every gram dried flower powder 1.5-6.0 ml water is soaked pollen, 24 hours duration.For reaching better immersion effect, soak water and preferably adopt deionized water.
The 4th step: the freezing processing of the pollen liquid after will soaking, temperature are 0 ℃～-30 ℃, 24 hours duration.
The 5th step: the pollen after freezing is put in the decompressor immediately, is decompressed to below-700 millimetress of mercury, keeps 15-20 minute.
The 6th step: use electric homogenizer homogenate, the homogenizer rotating speed is 2000-4000 rev/min.For guaranteeing the unlikely too high active material in the pollen that destroys of temperature of pollen liquid.Should note cooling during homogenate.Place frozen water such as the container that will hold pollen liquid, the temperature of general pollen liquid does not surpass 10 ℃, homogenate 3-5 minute.
The 7th step: with centrifuge the pollen wall in the Pollen homogenate is separated with nutriment, namely make the Normal juice of pure pollens nutrition material.
The 8th step: be storage, traffic requirements, also the Normal juice of pure pollens nutrition material can be made the crystalline powder of pure pollens nutrition material through lyophilization.
For further improving rate of breaking pollen wall, the process in above-mentioned the 4th, the 5th step can repeatedly repeat, and namely after reduced pressure treatment, carries out freezing processing again, reduced pressure treatment, and then carry out homogenate.Generally through freezing, reduced pressure treatment once, the sporoderm-broken rate of pollen can reach more than 95%, repeat freezing, reduced pressure treatment, then sporoderm-broken rate is higher.
It is pointed out that those parameters that occur in the above-mentioned example, the ratio of water and pollen during such as immersion, soak time, the pressure of freezing temperature, time, reduced pressure treatment, retention time etc. should not be understood as limitation of the present invention, and only are as an example selected.In practical operation, as long as adopted technological treatment of the present invention, namely pollen through soak, freezing, decompression, homogenized, no matter whether the control of parameter exceeds the above-mentioned parameter scope, all can reach the purpose that makes pollen broken wall.
Wall breaking technology of the present invention, technology is simple, need not special installation, and all operations all carries out at a lower temperature, does not only affect general nutriment in the pollen, and is conducive to preserve all active materials in the pollen.Especially the broken wall efficient height of this technology, and the pollens nutrition material is separated with pollen wall.
After adopting pollen wall breaking technology of the present invention, can make Normal juice or its crystalline powder of the pure pollens nutrition material of no pollen wall, this nutrition Normal juice crystalline powder is easy to human body and directly absorbs, and can supply the industries such as food, daily cosmetics, medicine to make various high-quality pollen products.
Claims (4)
1, a kind of wall breaking technology of pollen, earlier the removal of impurities of raw material pollen, grind to form single pollen grain, then through processing such as freezing grade, the invention is characterized in: the pollen of wearing into single pollen grain according to following order be soaked in water, freezing, decompression, homogenized, destroying pollen wall and the pollens nutrition thing is released in the water, and then separate through centrifuge, extract nutrients Normal juice in the pure pollen.
2, according to the pollen wall breaking technology of claim 1, it is characterized in that: said immersion water is deionized water, the ratio of pollen and water is every gram dried flower powder 1.5-6.0 ml water, soak time is 24 hours, said freezing temperature is 0 ℃～-30 ℃, 24 hours duration, said decompression is for to reduce to below 700 millimetress of mercury from normal pressure, kept 15-20 minute, during said homogenized, the homogenizer rotating speed is 2000-4000 rev/min, lowers the temperature with frozen water at the pollen liquid external container, keep pollen liquid temperature when homogenate not to be higher than 10 ℃, homogenate 3-5 minute.
3, according to the pollen wall breaking technology of claim 1, it is characterized in that: said freezing, reduced pressure treatment can repeat for several times.
4, according to the pollen wall breaking technology of claim 1 or 2 or 3, it is characterized in that: the pure pollens nutrition thing Normal juice that extracts is made the crystalline powder of pure pollens nutrition material through lyophilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85106076A CN85106076B (en) | 1985-08-09 | 1985-08-09 | pollen wall breaking technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85106076A CN85106076B (en) | 1985-08-09 | 1985-08-09 | pollen wall breaking technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85106076A CN85106076A (en) | 1987-02-25 |
| CN85106076B true CN85106076B (en) | 1987-12-09 |
Family
ID=4794867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN85106076A Expired CN85106076B (en) | 1985-08-09 | 1985-08-09 | pollen wall breaking technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN85106076B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055380C (en) * | 1995-04-24 | 2000-08-16 | 大连理工大学 | Wet bead grinding pollen-breaking technique |
| CN102229973B (en) * | 2011-06-15 | 2013-05-01 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN102793095A (en) * | 2012-08-21 | 2012-11-28 | 中国科学院西北高原生物研究所 | Rape bee pollen homogenizing wall-breaking method |
| CN104273409B (en) * | 2013-07-05 | 2017-07-28 | 中国农业科学院农产品加工研究所 | A kind of method for handling pollen |
-
1985
- 1985-08-09 CN CN85106076A patent/CN85106076B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN85106076A (en) | 1987-02-25 |
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| CF01 | Termination of patent right due to non-payment of annual fee |