CN1058393C - Extraction method of scorpion toxin crystal - Google Patents
Extraction method of scorpion toxin crystal Download PDFInfo
- Publication number
- CN1058393C CN1058393C CN97119704A CN97119704A CN1058393C CN 1058393 C CN1058393 C CN 1058393C CN 97119704 A CN97119704 A CN 97119704A CN 97119704 A CN97119704 A CN 97119704A CN 1058393 C CN1058393 C CN 1058393C
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- CN
- China
- Prior art keywords
- scorpion venom
- scorpio
- scorpion
- venom
- sterilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a method for extracting crystal scorpion venom. Scorpion venom is extracted, separated from impurities, dried and packaged at the critical temperature of 0 DEG C, so that the activity of scorpion venom enzyme is effectively retained, and scorpion venom protein is not damaged. The scorpion venom has strong toxicity and can be directly used for medical preparation, and the application range is wide.
Description
The present invention relates to a kind of extraction method of scorpion toxin.
At present extraction method of scorpion toxin mainly contains two kinds, and the one, to extract at normal temperatures, roguing, drying, its weak point is that virulence is low, poisonous protein is perishable.The 2nd, to carry out under subzero 10 ℃～30 ℃ conditions of low temperature, its weak point is that poisonous protein is destroyed when freezing, and virulence is low, and enzymatic activity is relatively poor.
Purpose of the present invention is exactly the deficiency that overcomes above-mentioned prior art, and a kind of extraction method of scorpion toxin crystal that can effectively keep the activity of scorpion venom enzyme, not destroy poisonous protein is provided.
The inventive method is: prepare (1), will put into aseptic extraction chamber through the Scorpio after sterilization, sterilization, the dust removal process, and the temperature of extraction chamber is 18～22 ℃; (2) carry poison, adopt the electric pulse device that Scorpio is carried out electric pulse stimulation, Scorpio is discharged venom in the body, fall in the container below the extraction chamber, this vessel temp is 0 ℃; (3) roguing, the method for employing centrifugalize is removed impurity and the dust in the scorpion venom under 0 ℃ of condition; (4) drying is put into hothouse with scorpion venom, adopts chemical method to carry out dried, and making scorpion venom dry is white crystals shape body, and baking temperature is 0 ℃; (5) sterilization encapsulation.
The used Scorpio of the present invention mainly is meant adult Scorpio.The temperature of extraction chamber is selected between 18～22 ℃, mainly is to consider that the Scorpio of growing up has vigor most, toxin expelling rate height under 18～22 ℃ of conditions.After scorpion venom was discharged, processed and encapsulation stored under 0 ℃ of condition of critical temperature all the time, and this is because we find in practice for many years, and the poisonous protein of scorpion venom can catabolism in the time of 0 ℃, enzyme active best, and virulence is the strongest.Consider in the practical operation that temperature control has certain error, scorpion venom is in whole extraction, roguing, drying and the process that encapsulates, and temperature should be controlled in 0 ℃ ± 0.5 ℃ the scope.The chemical seasoning method that the present invention adopts is to put into chemical drier in hothouse, and the moisture content in the scorpion venom is adsorbed to fall, and making scorpion venom dry is white crystals shape body.
Find that through test for many years the frequency of used electric pulse device is per minute 85～95 times, effect was best when voltage was 22～28 volts, both can improve the toxin expelling rate of Scorpio, can significantly reduce the dead and wounded quantity of Scorpio again.Scorpio after the inventive method is extracted is cultured 2～4 Zhou Houke and carries poison once more.
The inventive method is put forward malicious method and compared with original, has effectively improved the quality of the scorpion venom of putting forward, and makes that its poisonous protein is not destroyed, enzymatic activity is good, virulence is strong, can be directly used in pharmacy, and is of many uses.
Claims (1)
1, a kind of extraction method of scorpion toxin crystal is characterized in that: prepare (1), will put into aseptic extraction chamber through the Scorpio after sterilization, sterilization, the dust removal process, and the temperature of extraction chamber is 18～22 ℃; (2) carry poison, adopt the electric pulse device that Scorpio is carried out electric pulse stimulation, Scorpio is discharged venom in the body, fall in the container below the extraction chamber, this vessel temp is 0 ℃; The frequency of used electric pulse device is that per minute 85～95 times, voltage are 22～28 volts; (3) roguing, the method for employing centrifugalize is removed impurity and the dust in the scorpion venom under 0 ℃ of condition; (4) drying is put into hothouse with scorpion venom, adopts chemical method to be carried out at dry processing, and making scorpion venom dry is white crystals shape body, and baking temperature is 0 ℃; (5) sterilization encapsulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97119704A CN1058393C (en) | 1997-09-26 | 1997-09-26 | Extraction method of scorpion toxin crystal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97119704A CN1058393C (en) | 1997-09-26 | 1997-09-26 | Extraction method of scorpion toxin crystal |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1176106A CN1176106A (en) | 1998-03-18 |
| CN1058393C true CN1058393C (en) | 2000-11-15 |
Family
ID=5175511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97119704A Expired - Fee Related CN1058393C (en) | 1997-09-26 | 1997-09-26 | Extraction method of scorpion toxin crystal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1058393C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080992C (en) * | 1998-06-12 | 2002-03-20 | 程满温 | Process for extracting scorpion toxin |
| CN102451194A (en) * | 2010-10-25 | 2012-05-16 | 马震 | Extraction method for scorpion venom |
| CN102308775B (en) * | 2011-07-12 | 2013-11-13 | 徐建明 | Scorpion venom extractor |
| CN103285039A (en) * | 2013-06-16 | 2013-09-11 | 杨文川 | Biotoxin living body heating and extracting method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2031605U (en) * | 1988-05-19 | 1989-02-01 | 杨大庆 | Extractor for continual extraction of scorpion's poison |
-
1997
- 1997-09-26 CN CN97119704A patent/CN1058393C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2031605U (en) * | 1988-05-19 | 1989-02-01 | 杨大庆 | Extractor for continual extraction of scorpion's poison |
Non-Patent Citations (1)
| Title |
|---|
| 《特产研究》1992,(2) 1992.1.1 翟永功等，动物毒素的采集加工 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1176106A (en) | 1998-03-18 |
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| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |