CN109568254A - A kind of Plancenta Histolysate's injection and preparation method thereof - Google Patents
A kind of Plancenta Histolysate's injection and preparation method thereof Download PDFInfo
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- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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Abstract
The invention discloses a kind of Plancenta Histolysate's injections and preparation method thereof, present invention application placenta tissue carries out separating twice by frozen dried again and obtains placenta tissue dry powder, avoiding placenta tissue in conventional heating drying drying process and being easy to happen to go bad leads to toxin, the growth problems of bacterium, in conjunction with acidolysis, centrifuge separation etc., sour water solution biostimulin has been made, relative to conventional multigelation, filtering etc., purification efficiency is saved, further adsorbed by protein adsorption column, elution, purer active peptide is obtained, improve the purity of active peptide.The preparation step is simple, is easy to commercial introduction, application value with higher.Moreover, it adsorbed, eluted through protein adsorption column, the content of the active material in gained Plancenta Histolysate is higher, and it is more convenient to use in medical treatment, and Plancenta Histolysate's injection that various concentration is made respectively, more flexible convenience can be dispensed.
Description
Technical field
The present invention relates to Plancenta Histolysates, and in particular, to a kind of Plancenta Histolysate's injection and preparation method thereof.
Background technique
Biostimulin is the placenta by healthy parent after inspection and quarantine, by special separation and Extraction means, by tire
Active ingredients in disk are manufactured after extracting to have medical, health care, beauty, the injection preparation of health and other effects.Due to
Contain more than 8000 kinds of primitive life substances in placenta, there are more than the 270 kinds of original enzyme materials of the mankind that can be named at present, so right
Extraction process has very high requirement.Production technology includes polymolecular separating and filtering technology, nanometer purification technique, carbon dioxide skill
Art, under the guarantee of above-mentioned technical conditions, the molecular weight of most crucial ingredient can reach the molecular weight of micromolecule polypeptide, thus completely
It is absorbed by the human body.
Pure placental preparations are sci-tech products, have wrinkle reduction, antifatigue, healing in terms of beauty through scientific validation
The various functions such as wound, eliminate chloasma and various color spots, dark sore, these have all been confirmed by scientific experiment.Its activity
Effect enhances histiocytic metabolic function, the defective mode of body is equilibrated to normal point, to make body immune
Ability is continuously improved, and delays senescence.This science beauty curing is better than the beauty method promoted currently on the market, is to be worth China
The new method that beauty circle personage tries out can also become 21 century international green beauty new trend.
And the extracting method of conventional Plancenta Histolysate is more using drying combination to tissue fluid generally after soda acid decomposition
Secondary freeze thawing is filtered for multiple times or the multiple means such as ultrafiltration, supercritical extract, to extract the active peptides in biostimulin, still,
In the process, the decline that frequently can lead to effective active polypeptide inactivation or active constituent content, causes precious placenta raw material
Waste.Therefore, active peptides in biostimulin can effectively be extracted by how providing one kind, and reduce toxin, bacterium taste
Raw, the method for improving the content of active material in Plancenta Histolysate is current urgent problem to be solved.
Summary of the invention
The object of the present invention is to provide a kind of Plancenta Histolysate's injections and preparation method thereof, and this method can be mentioned effectively
Active peptides in biostimulin are taken, and reduce toxin, bacteria breed, improve the content of active material in Plancenta Histolysate
It is current urgent problem to be solved with the concentration in preparation.
To achieve the goals above, the present invention provides a kind of preparation methods of Plancenta Histolysate's injection, including with
Lower step: (1) choosing the placenta of health, and cleaning removes watery blood and dirt, rinsed, obtained pretreated in sodium chloride solution
Placenta tissue；(2) pretreated placenta tissue is once crushed, is then carried out at freeze-drying using Vacuum Freezing & Drying Technology
Reason, then separating twice is carried out, obtain placenta tissue dry powder；(3) placenta tissue dry powder is mixed with water for injection, salt is then added
Acid solution, the pH value for adjusting mixed liquor is 2.6~3.6, obtains sour mixed liquor；(4) heating of sour mixed liquor is hydrolyzed, then into
Row centrifuge separation, obtains sour water solution biostimulin；(5) sour water solution biostimulin protein adsorption column is passed through to be adsorbed, wash
De- step obtains purifying biostimulin；(6) pH to 6.7-7.3 is adjusted to purifying biostimulin sodium hydroxide, and gone out
Bacterium.
In the above-mentioned technical solutions, the present invention is frozen using pretreated placenta tissue by Vacuum Freezing & Drying Technology
Dry-cure carries out separating twice again and obtains placenta tissue dry powder, avoids placenta tissue in conventional heating drying drying process and is easy
The rotten growth problems for leading to toxin, bacterium occur, in conjunction with acidolysis, sour water solution biostimulin has been made in centrifuge separation etc.,
Relative to conventional multigelation, filtering etc., purification efficiency has been saved, further adsorbed, eluted by protein adsorption column,
Purer active peptide has been obtained, the purity of active peptide is improved.Preparation step of the present invention is simple, is easy to commercial introduction, have compared with
High application value.Moreover, which is adsorbed through protein adsorption column, is eluted, in gained Plancenta Histolysate
Active material content it is higher, can through be dispensed respectively be made various concentration Plancenta Histolysate's injection, dispense it is more flexible,
It is more convenient to use in medical treatment.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
A kind of preparation method of Plancenta Histolysate's injection, comprising the following steps:
(1) placenta of health is chosen, cleaning removes watery blood and dirt, rinsed, pre-processed in sodium chloride solution
Placenta tissue；
(2) pretreated placenta tissue is once crushed, is then carried out at freeze-drying using Vacuum Freezing & Drying Technology
Reason, then separating twice is carried out, obtain placenta tissue dry powder；
(3) placenta tissue dry powder is mixed with water for injection, hydrochloric acid solution is then added, the pH value for adjusting mixed liquor is
2.6~3.6, obtain sour mixed liquor；
(4) heating of sour mixed liquor is hydrolyzed, then be centrifuged, obtain sour water solution biostimulin；
(5) sour water solution biostimulin is passed through the step of protein adsorption column is adsorbed, eluted, obtains purifying placenta group
Knit liquid；
(6) pH to 6.7-7.3 is adjusted to purifying biostimulin sodium hydroxide, and sterilized.
In the above-mentioned technical solutions, the present invention is frozen using pretreated placenta tissue by Vacuum Freezing & Drying Technology
Dry-cure carries out separating twice again and obtains placenta tissue dry powder, avoids placenta tissue in conventional heating drying drying process and is easy
The rotten growth problems for leading to toxin, bacterium occur, in conjunction with acidolysis, sour water solution biostimulin has been made in centrifuge separation etc.,
Relative to conventional multigelation, filtering etc., purification efficiency has been saved, further adsorbed, eluted by protein adsorption column,
Purer active peptide has been obtained, the purity of active peptide is improved.Preparation step of the present invention is simple, is easy to commercial introduction, have compared with
High application value.Moreover, which is adsorbed through protein adsorption column, is eluted, in gained Plancenta Histolysate
Active material content it is higher, can through be dispensed respectively be made various concentration Plancenta Histolysate's injection, dispense it is more flexible,
It is more convenient to use in medical treatment.
It further include repeating to be added by centrifugation obtained in step (4) in a kind of preferred embodiment of the present invention
Acid-hydrolyzed step is carried out in sour mixed liquor.In this way, placenta tissue can be improved further to complete human placenta is not extracted
Utilization rate.
In a kind of preferred embodiment of the present invention, the weight ratio of placenta tissue dry powder and water for injection in step (3)
It is 1: 8-15.In this way, the hydrolysis efficiency of placenta tissue can be improved, and in subsequent centrifugal separation processes, by extracting solution and group
Fragment is knitted to efficiently separate.
In a kind of preferred embodiment of the present invention, the molar concentration of hydrochloric acid solution is 1~2mol/L in rapid (3), this
Sample is conducive to effective control to pH.
In a kind of preferred embodiment of the present invention, the condition of frozen dried includes: that will crush placenta tissue to be put into very
In vacuum freecing-dry machine, close hermatic door, open vacuum pump switch and freeze-dryer switch, be evacuated to 18kPa~
28kPa, while starting refrigeration, the condenser temperature of vacuum freeze drier is down to -45 DEG C~-60 DEG C in 2~4 hours, is protected
Hold the time 2~5 hours；Product is slowly heated by the heating system under partition, delivery article is needed for the sublimation process
Heat will crush placenta tissue in 10~25 hours and be warming up to 0~15 DEG C, make to crush placenta tissue drying, obtain placenta group
Knit dry powder.
In a kind of preferred embodiment of the present invention, the hydrolysising condition in the step (4) includes that temperature is 35~45
DEG C, and temperature is kept to hydrolyze 40~50min.In the process, can make to crush placenta tissue rapid draing and dried
The generation of bacterium and toxin is prevented in journey, also, keeps the activity of the active material in placenta tissue.
In a kind of preferred embodiment of the present invention, the filler in the protein adsorption column includes that Sephadex series is solidifying
Glue filter packing, Superdex series gel filtration filler, Sephacryl series gel filtration filler, Sepharose series are solidifying
Glue filter packing, Superose series gel filtration filler, Sepharose pedestal ion-exchange packing, Source pedestal ion
One of filler, Capto pedestal ion-exchange packing and Sephadex pedestal ion-exchange packing are exchanged, these are commercially available
Product repeat no more.In this way, being adsorbed, being eluted, activated protein or polypeptide can be efficiently separated, and during elution, passes through color
Spectral analysis method carries out centralized collection to elution absorption peak, improves the concentration of active material in collection liquid, be conducive to later period preparation
Packing or concentration proportion.
And for the eluent in elution process, can be selected in a wider range, for example, eluent be pH 1.0~
11.0 buffer solution, including following one or more: maleate buffer, malonic acid buffer, citrate buffer solution, cream
Acid buffer, formic acid buffer, succinic acid buffer solution, acetate buffer, malonic acid buffer, phosphate buffer, piperazine are slow
Fliud flushing, L-Histidine buffer, Tris buffer, Triethanolamine buffer, diethanolamine buffer, ethanol amine buffer and
Diamine Propane buffer.These, can be according to the explanation of absorption packing material, and the selection being directed to, details are not described herein.
The step of pH is adjusted in a kind of preferred embodiment of the present invention, in step (6) further includes when purifying placenta group
The sodium hydroxide solution that molar concentration is 0.5mol/L is added when knitting liquid pH value less than 6.7, in Xiang Chunhua biostimulin, makes it
PH value is 6.7-7.3,
It is 1.0mol/ that molar concentration is added when purifying biostimulin's pH value greater than 7.3, in Xiang Chunhua biostimulin
The hydrochloric acid of L makes its pH value 6.7-7.3.In this way, the pH of preparation obtained and the pH of human body are close, can directly support direct
Ejection preparation can also add physiological saline etc. and be adjusted to the concentration of preparation.
In a kind of preferred embodiment of the present invention, sterilizing methods can be pasteurization, dry heating method, film filtering
One of method and incubation at low pH value are a variety of, these are conventional technical means, in embodiment later, in order to save reality
The time is tested, is illustrated only with a kind of embodiment.
The present invention also provides a kind of Plancenta Histolysate's injections being prepared according to previously described preparation method.
In the above-mentioned technical solutions, the present invention is frozen using pretreated placenta tissue by Vacuum Freezing & Drying Technology
Dry-cure carries out separating twice again and obtains placenta tissue dry powder, avoids placenta tissue in conventional heating drying drying process and is easy
The rotten growth problems for leading to toxin, bacterium occur, in conjunction with acidolysis, sour water solution biostimulin has been made in centrifuge separation etc.,
Relative to conventional multigelation, filtering etc., purification efficiency has been saved, further adsorbed, eluted by protein adsorption column,
Purer active peptide has been obtained, the purity of active peptide is improved.Preparation step of the present invention is simple, is easy to commercial introduction, have compared with
High application value.Moreover, which is adsorbed through protein adsorption column, is eluted, in gained Plancenta Histolysate
Active material content it is higher, can through be dispensed respectively be made various concentration Plancenta Histolysate's injection, dispense it is more flexible,
It is more convenient to use in medical treatment.
The present invention will be described in detail by way of examples below.Protein in following Plancenta Histolysate's injections
The application BCA method of content measures protein content, i.e., first draws standard curve with standard protein solution and absorbance value, then
The protein concentration in solution to be measured is calculated with the absorbance value of solution to be measured.This is ordinary skill in the art means,
This is repeated no more.
Embodiment 1
A kind of preparation method of Plancenta Histolysate's injection, comprising the following steps:
(1) placenta for collecting healthy puerpera's term birth, after the virus examinations such as hepatitis B, hepatitis, AIDS, syphilis, sieve
Selecting virus examination result is negative placenta as raw material, and spare, watery blood and dirt are removed in cleaning, are floated in sodium chloride solution
It washes, obtains pretreated placenta tissue；
(2) pretreated placenta tissue is once crushed, is then carried out at freeze-drying using Vacuum Freezing & Drying Technology
Reason, the condition of frozen dried include: that will crush placenta tissue to be put into vacuum freeze drier, close hermatic door, open vacuum
Switch pump and freeze-dryer switch, are evacuated to 18kPa, while starting refrigeration, by vacuum freeze drier in 2 hours
Condenser temperature is down to -45 DEG C, the retention time 5 hours；Product is slowly heated by the heating system under partition, delivery article exists
Heat needed for sublimation process will crush placenta tissue in 10 hours and be warming up to 0 DEG C, make to crush placenta tissue drying, then
Separating twice is carried out, placenta tissue dry powder is obtained；
(3) placenta tissue dry powder is mixed with water for injection with weight ratio for 1: 8,1mol/L hydrochloric acid solution is then added,
The pH value for adjusting mixed liquor is 2.6, obtains sour mixed liquor；
(4) 40min is hydrolyzed in 35 DEG C for sour mixed liquor, then be centrifugated with 15000rpm, obtain sour water solution tire
Disk tissue fluid；Centrifugation, which repeats to be added in sour mixed liquor, carries out sour water solution；
(5) pH value of sour water solution biostimulin is adjusted to 4.0, and column is filled using SP-Sepharose HP, chromatographs column volume
40mL.Pillar first is balanced with the formic acid buffer of pH 4.0, balances 10 column volumes, 4.0 formic acid of pH for being 0 to 4M with concentration
Buffer carries out gradient elution, collects each peak on map, obtains purifying biostimulin；
(6) pH to 6.7 is adjusted to purifying biostimulin sodium hydroxide, and biostimulin is quantified with every bottle of 1ml
It is sub-packed in the ampoule bottle of 1.5ml, was then built in whole sterilizing cabinet in 2 hours, carry out sterilization treatment, obtain biostimulin
Injection, wherein sterilization treatment temperature is 114 DEG C, and sterilization time is 30 minutes.
The pH of biostimulin's injection obtained is 6.7, protein content 28mg/mL.
Embodiment 2
A kind of preparation method of Plancenta Histolysate's injection, comprising the following steps:
(1) placenta for collecting healthy puerpera's term birth, after the virus examinations such as hepatitis B, hepatitis, AIDS, syphilis, sieve
Selecting virus examination result is negative placenta as raw material, and spare, watery blood and dirt are removed in cleaning, are floated in sodium chloride solution
It washes, obtains pretreated placenta tissue；
(2) pretreated placenta tissue is once crushed, is then carried out at freeze-drying using Vacuum Freezing & Drying Technology
Reason, the condition of frozen dried include: that will crush placenta tissue to be put into vacuum freeze drier, close hermatic door, open vacuum
Switch pump and freeze-dryer switch, are evacuated to 28kPa, while starting refrigeration, by vacuum freeze drier in 4 hours
Condenser temperature is down to -60 DEG C, the retention time 2 hours；Product is slowly heated by the heating system under partition, delivery article exists
Heat needed for sublimation process will crush placenta tissue in 25 hours and be warming up to 15 DEG C, make to crush placenta tissue drying, then
Separating twice is carried out, placenta tissue dry powder is obtained；
(3) placenta tissue dry powder is mixed with water for injection with weight ratio for 1: 15,2mol/L hydrochloric acid solution is then added,
The pH value for adjusting mixed liquor is 3.6, obtains sour mixed liquor；
(4) 50min is hydrolyzed in 45 DEG C for sour mixed liquor, then be centrifugated with 15000rpm, obtain sour water solution tire
Disk tissue fluid；Centrifugation, which repeats to be added in sour mixed liquor, carries out sour water solution；
(5) pH value of sour water solution biostimulin is adjusted to 7.8, and column is filled using Q agarose, chromatographs column volume 50mL, first
Balance pillar with the phosphate buffer of pH 7.8, balance 10 column volumes, with concentration be 0 to 2M 7.8 phosphate buffer of pH into
Row gradient elution collects each peak on map, obtains purifying biostimulin；
(6) pH to 7.3 is adjusted to purifying biostimulin sodium hydroxide, and biostimulin is quantified with every bottle of 1ml
It is sub-packed in the ampoule bottle of 1.5ml, was then built in whole sterilizing cabinet in 2 hours, carry out sterilization treatment, obtain biostimulin
Injection, wherein sterilization treatment temperature is 114 DEG C, and sterilization time is 30 minutes.
The pH of biostimulin's injection obtained is 7.3, protein content 32mg/mL.
Embodiment 3
A kind of preparation method of Plancenta Histolysate's injection, comprising the following steps:
(1) placenta for collecting healthy puerpera's term birth, after the virus examinations such as hepatitis B, hepatitis, AIDS, syphilis, sieve
Selecting virus examination result is negative placenta as raw material, and spare, watery blood and dirt are removed in cleaning, are floated in sodium chloride solution
It washes, obtains pretreated placenta tissue；
(2) pretreated placenta tissue is once crushed, is then carried out at freeze-drying using Vacuum Freezing & Drying Technology
Reason, the condition of frozen dried include: that will crush placenta tissue to be put into vacuum freeze drier, close hermatic door, open vacuum
Switch pump and freeze-dryer switch, are evacuated to 23kPa, while starting refrigeration, by vacuum freeze drier in 3 hours
Condenser temperature is down to 50 DEG C, the retention time 3.5 hours；Product is slowly heated by the heating system under partition, delivery article
The heat needed for sublimation process will crush placenta tissue in 18 hours and be warming up to 8 DEG C, make to crush placenta tissue drying,
Separating twice is carried out again, obtains placenta tissue dry powder；
(3) placenta tissue dry powder is mixed with water for injection with weight ratio for 1: 12, it is molten that 1.5mol/L hydrochloric acid is then added
Liquid, the pH value for adjusting mixed liquor is 3, obtains sour mixed liquor；
(4) 45min is hydrolyzed in 40 DEG C for sour mixed liquor, then be centrifugated with 15000rpm, obtain sour water solution tire
Disk tissue fluid；Centrifugation, which repeats to be added in sour mixed liquor, carries out sour water solution；
(5) pH value of sour water solution biostimulin is adjusted to 8.0, and column is filled using DEAE-Sepharose FF, chromatographs cylinder
Product 40mL.Pillar first is balanced with the phosphate buffer of pH 8.0, balances 10 column volumes, 8.0 phosphorus of pH for being 0 to 3M with concentration
Acid buffer carries out gradient elution, collects each peak on map, obtains purifying biostimulin；
(6) pH to 7 is adjusted to purifying biostimulin sodium hydroxide, and biostimulin is quantitatively divided with every bottle of 1ml
It in ampoule bottle loaded on 1.5ml, was then built in whole sterilizing cabinet in 2 hours, carries out sterilization treatment, obtain biostimulin's note
Agent is penetrated, wherein sterilization treatment temperature is 114 DEG C, and sterilization time is 30 minutes.
The pH of biostimulin's injection obtained is 7, protein content 25mg/mL.
Embodiment 4
Preparation method is in the same manner as in Example 3, the difference is that step (5) carries out as follows:
The pH value of sour water solution biostimulin is adjusted to 10.0, column is filled using Q-Sepharose FF.First with pH's 10.0
Phosphate buffer balances pillar, balances 10 column volumes, carries out linear ladder with 10.0 phosphate buffer of pH that concentration is 0 to 2M
Degree elution collects each peak according to map, obtains purifying biostimulin.
The pH of biostimulin's injection obtained is 7, protein content 36mg/mL.
Embodiment 5
Preparation method is same as Example 3, unlike, step (5) is according to following progress:
The pH value of sour water solution biostimulin is adjusted to 2.0, column is filled using S-SOURCE 30, chromatographs column volume 40mL.First
Pillar is balanced with the maleate buffer of pH 2.0, balances 10 column volumes, it is slow with 2.0 maleic acid of pH that concentration is 0 to 10M
Fliud flushing carries out gradient elution, collects each peak on map, obtains purifying biostimulin.
The pH of biostimulin's injection obtained is 7, protein content 24g/L.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of preparation method of Plancenta Histolysate's injection, which comprises the following steps:
(1) placenta of health is chosen, cleaning removes watery blood and dirt, rinsed in sodium chloride solution, obtain pretreated tire
Disk tissue；
(2) pretreated placenta tissue is once crushed, frozen dried is then carried out using Vacuum Freezing & Drying Technology, then
Separating twice is carried out, placenta tissue dry powder is obtained；
(3) placenta tissue dry powder is mixed with water for injection, hydrochloric acid solution is then added, adjust mixed liquor pH value be 2.6~
3.6, obtain sour mixed liquor；
(4) heating of sour mixed liquor is hydrolyzed, then be centrifuged, obtain sour water solution biostimulin；
(5) sour water solution biostimulin is passed through the step of protein adsorption column is adsorbed, eluted, obtains purifying placenta tissue
Liquid；
(6) pH to 6.7-7.3 is adjusted to purifying biostimulin sodium hydroxide, and sterilized.
2. preparation method according to claim 1, wherein further include repeating to add by centrifugation obtained in step (4)
Enter and carries out acid-hydrolyzed step in sour mixed liquor.
3. preparation method according to claim 1 or 2, wherein the weight of placenta tissue dry powder and water for injection in step (3)
Amount is than being 1: 8-15.
4. preparation method according to claim 3, wherein the molar concentration of hydrochloric acid solution is 1~2mol/L in rapid (3).
5. preparation method according to claim 1, wherein the condition of frozen dried includes: that will crush placenta tissue to be put into
In vacuum freeze drier, close hermatic door, open vacuum pump switch and freeze-dryer switch, be evacuated to 18kPa~
28kPa, while starting refrigeration, the condenser temperature of vacuum freeze drier is down to -45 DEG C~-60 DEG C in 2~4 hours, is protected
Hold the time 2~5 hours；Product is slowly heated by the heating system under partition, delivery article is needed for the sublimation process
Heat will crush placenta tissue in 10~25 hours and be warming up to 0~15 DEG C, make to crush placenta tissue drying, obtain placenta group
Knit dry powder.
6. preparation method according to claim 1 or 2, wherein the hydrolysising condition in the step (4) includes that temperature is 35
~45 DEG C, and temperature is kept to hydrolyze 40~50min.
7. according to right to have 1 or 2 described in preparation method, wherein the filler in the protein adsorption column includes Sephadex
Serial gel filtration filler, Superdex series gel filtration filler, Sephacryl series gel filtration filler, Sepharose
Serial gel filtration filler, Superose series gel filtration filler, Sepharose pedestal ion-exchange packing, Source base
One of frame ion-exchange packing, Capto pedestal ion-exchange packing and Sephadex pedestal ion-exchange packing.
8. according to right to have 1 or 2 described in preparation method, wherein the step of pH is adjusted in step (6) further includes when purifying tire
The sodium hydroxide solution that molar concentration is 0.5mol/L is added when disk tissue fluid pH value is less than 6.7, in Xiang Chunhua biostimulin,
Make its pH value 6.7-7.3,
It is 1.0mol/L's that molar concentration is added when purifying biostimulin's pH value greater than 7.3, in Xiang Chunhua biostimulin
Hydrochloric acid makes its pH value 6.7-7.3.
9. according to right to have 1 or 2 described in preparation method, wherein sterilizing methods be pasteurization, dry heating method, film mistake
One of filter method and incubation at low pH value are a variety of.
10. Plancenta Histolysate's injection that -9 described in any item preparation methods are prepared according to claim 1.
Priority Applications (1)
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| CN115708798A (en) * | 2023-01-10 | 2023-02-24 | 昆明时光肌生物技术有限公司 | One-step hydrolysis preparation method of sheep placenta extract |
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