CN1248442A - An anticancer marine organism extract - Google Patents
An anticancer marine organism extract Download PDFInfo
- Publication number
- CN1248442A CN1248442A CN98110478A CN98110478A CN1248442A CN 1248442 A CN1248442 A CN 1248442A CN 98110478 A CN98110478 A CN 98110478A CN 98110478 A CN98110478 A CN 98110478A CN 1248442 A CN1248442 A CN 1248442A
- Authority
- CN
- China
- Prior art keywords
- extract
- anticancer
- marine organism
- water
- marine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001093 anti-cancer Effects 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 241000628923 Anadara sativa Species 0.000 claims abstract description 4
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 4
- 241001339782 Scapharca broughtonii Species 0.000 claims description 3
- 238000000502 dialysis Methods 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 11
- 238000002360 preparation method Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 241000180579 Arca Species 0.000 abstract description 2
- 102000003886 Glycoproteins Human genes 0.000 abstract description 2
- 108090000288 Glycoproteins Proteins 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 238000002512 chemotherapy Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 238000011275 oncology therapy Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- MWWSFMDVAYGXBV-RUELKSSGSA-N Doxorubicin hydrochloride Chemical compound Cl.O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 MWWSFMDVAYGXBV-RUELKSSGSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000002256 antimetabolite Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000011254 conventional chemotherapy Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229960002918 doxorubicin hydrochloride Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 201000005296 lung carcinoma Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
An anticancer marine organism extract is characterized in that the extract is obtained by soaking crushed marine organisms in water and hydrolyzing at low temperature. The marine organism is Arca subcrenata or Arca inflata Reeve. The invention has the advantages of no damage to glycoprotein and other active substances, simple and feasible process, abundant and easily available domestic medicine sources, low cost, no environmental pollution, obvious curative effect of the preparation in anticancer clinical application and capability of improving the immunocompetence of patients.
Description
The present invention relates to a kind of medicine, particularly relate to the anti-cancer active matter that a kind of water is extracted by marine organisms.
Cancer has become the important killer of human health, and treatment for cancer is not had specific medicament as yet.Current treatment method for cancer mainly is excision, radiation and chemotherapy.The most medicine that chemotherapy is used is synthetic, all has than strong cytotoxicity or antimetabolism.Some biological anticancer preparations also appear in 1980's, for example interferon (IFN), glug cell etc., though these preparations all have certain curative effect at anticancer aspect, but they are at medicine source side face, be subjected to the limitation of many biological engineering conditions in extraction, cultivation, amplification, the preparation difficulty is big, the cost height, cost an arm and a leg, the patient is difficult to bear.
The purpose of this invention is to provide the anticancer marine organism extract of a kind of energy, it can remedy the above-mentioned deficiency of existing cancer therapy drug.
Extract of the present invention is characterized in that it is the extract that chippy marine organisms obtain through low temperature hydrolysis that is soaked in water.
Produce the technology of extract of the present invention, it is characterized in that marine organisms are cleaned, rub, are soaked in water, through low temperature hydrolysis is centrifugal remove slag after, again with supernatant dialysis, last concentrated frozen drying.
Advantage of the present invention is that water at low temperature is carried and do not destroyed required glycoprotein isoreactivity material, technology simple possible; The medicine source is abundant at home, is easy to get, and cost is low; Do not cause environmental pollution; This preparation is evident in efficacy in anticancer application clinically, and can improve patient's immunocompetence.
Below by embodiment the present invention is described
Get the meat of for example two shellfish Scapharca subcrenatas of marine organisms or Scapharca broughtonii, with pulverizer it is rubbed, granularity is about 0.5cm, ratio in Carnis Arca inflata meat and water 1: 1-4 for example adds water at 1: 2, make its mix homogeneously, form the grain slurry gradually, subsequently in low temperature 0-4 ℃ refrigerator at a slow speed (for example 60 change/hour) stirred 1-3 hour, make its abundant hydrolysis; Remove slag in centrifuge then, get supernatant after centrifugal, carry out secondary centrifuging, the liquid temperature control is about 20 minutes at 1-4 ℃ between institute lasts.Get supernatant and dialyse, in 24 hours, change redistilled water 1-3 time of bag filter periphery by bag filter.Extracting solution after the dialysis carries out lyophilization with freezer dryer and promptly obtains powdered extract of the present invention.The marine organism extract of making by this technology can keep biological activity.Can select for use sodium chloride normal saline or glucose to make solvent during use, prepare in the ratio of every milliliter of 10mg freezing dry powder.Conventional injection need go bacterium in the source of reducing phlegm and internal heat, and carries out aseptic embedding then.This product can be deposited under 1-10 ℃.
The marine organisms that the present invention is used, Scapharca subcrenata or Scapharca broughtonii requirement used in the present embodiment are fresh, shell fully, and decontamination rinsing three times was soaked 10 minutes again, drenched to do, and reuse distilled water drip washing three times is drenched dried standby.
Made the cancer therapy drug sensitive experiment with medicine of the present invention, in the In vitro culture drug sensitive experiment of 60 routine human cancer cells, demonstrating has damage, regression and downright bad effect to cancerous cell, has stronger kill capability, total effective rate reaches 55.4%, kill rate to lung carcinoma cell, breast cancer cell, stomach cancer cell reaches 94%, 68% and 53.1% respectively.Show that in correlated experiment product of the present invention is better than conventional chemotherapy medicines such as cyclophosphamide, doxorubicin hydrochloride significantly.
Another experiment shows that extract of the present invention is all demonstrating higher immune level aspect characteristic immunity and the nonspecific immunity.
Claims (3)
1, the anticancer marine organism extract of a kind of energy is characterized in that it is the extract that chippy marine organisms obtain through low temperature hydrolysis that is soaked in water.
2, implement the used technology of claim 1, it is characterized in that marine organisms are cleaned, rub, are soaked in water, after low temperature hydrolysis, centrifugal removing slag, again with the supernatant dialysis, last concentrated frozen drying.
3, extract as claimed in claim 1 is characterized in that described marine organisms are Scapharca subcrenata or Scapharca broughtonii.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98110478A CN1089241C (en) | 1998-09-18 | 1998-09-18 | Anticancer extract of Arca subcrenata or Arca inflata Reeve |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98110478A CN1089241C (en) | 1998-09-18 | 1998-09-18 | Anticancer extract of Arca subcrenata or Arca inflata Reeve |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1248442A true CN1248442A (en) | 2000-03-29 |
| CN1089241C CN1089241C (en) | 2002-08-21 |
Family
ID=5220526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98110478A Expired - Fee Related CN1089241C (en) | 1998-09-18 | 1998-09-18 | Anticancer extract of Arca subcrenata or Arca inflata Reeve |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1089241C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371347C (en) * | 2003-09-22 | 2008-02-27 | 于荣敏 | Active material of polypeptide proteins in bloody clam, preparation method and usage |
| CN101416986B (en) * | 2008-12-10 | 2011-09-21 | 青岛海生肿瘤医院 | Arca extract enteric-coated capsules and manufacture method thereof |
| CN102716153A (en) * | 2012-06-27 | 2012-10-10 | 陈顺泰 | Compound drug for treating hyperplasia of mammary glands and method for producing same |
| CN103254303A (en) * | 2013-04-01 | 2013-08-21 | 于荣敏 | Preparation method and application of antineoplastic active polypeptide component in Arca subcrenata Lischke |
| CN103254302A (en) * | 2013-04-01 | 2013-08-21 | 于荣敏 | Method for separation of antitumor polypeptide compound from Arca subcrenata Lischke and application |
| CN107569445A (en) * | 2017-10-20 | 2018-01-12 | 烟台宇诚企业管理咨询有限公司 | A kind of Haircare composition based on marine organism extract and preparation method thereof |
| CN108497120A (en) * | 2018-05-29 | 2018-09-07 | 广州聚禅现代农业研究院有限公司 | A kind of clearing heat and promoting diuresis tea and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1132646A (en) * | 1995-10-19 | 1996-10-09 | 黄国能 | Oyster-spirulina capsule and its prepn process |
| CN1062125C (en) * | 1995-10-26 | 2001-02-21 | 中山大学 | Tooth-paste and gargle containing water solutable component of pearl |
-
1998
- 1998-09-18 CN CN98110478A patent/CN1089241C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371347C (en) * | 2003-09-22 | 2008-02-27 | 于荣敏 | Active material of polypeptide proteins in bloody clam, preparation method and usage |
| CN101416986B (en) * | 2008-12-10 | 2011-09-21 | 青岛海生肿瘤医院 | Arca extract enteric-coated capsules and manufacture method thereof |
| CN102716153A (en) * | 2012-06-27 | 2012-10-10 | 陈顺泰 | Compound drug for treating hyperplasia of mammary glands and method for producing same |
| CN102716153B (en) * | 2012-06-27 | 2014-10-08 | 陈顺泰 | Compound drug for treating hyperplasia of mammary glands and method for producing same |
| CN103254303A (en) * | 2013-04-01 | 2013-08-21 | 于荣敏 | Preparation method and application of antineoplastic active polypeptide component in Arca subcrenata Lischke |
| CN103254302A (en) * | 2013-04-01 | 2013-08-21 | 于荣敏 | Method for separation of antitumor polypeptide compound from Arca subcrenata Lischke and application |
| CN107569445A (en) * | 2017-10-20 | 2018-01-12 | 烟台宇诚企业管理咨询有限公司 | A kind of Haircare composition based on marine organism extract and preparation method thereof |
| CN108497120A (en) * | 2018-05-29 | 2018-09-07 | 广州聚禅现代农业研究院有限公司 | A kind of clearing heat and promoting diuresis tea and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1089241C (en) | 2002-08-21 |
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Legal Events
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| C49 | Reinstatement of patent right or utility model | ||
| C56 | Change in the name or address of the patentee |
Owner name: QINGDAO GUOTAI( GROUP ) CO., LTD. Free format text: FORMER NAME: LIN HUAYING |
|
| CP03 | Change of name, title or address |
Address after: 7, four stream Road, Sifang District, Qingdao, Shandong Province, zip code: 266031 Co-patentee after: Lin Huaying Patentee after: Qingdao Guotai (Group) Co.,Ltd. Address before: Shandong province Qingdao sea Riyin Biological Products Co. Ltd., zip code: 266003 Patentee before: Lin Huaying |
|
| RR01 | Reinstatement of patent right | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20020821 Termination date: 20091019 |