CN2917269Y - Experiment shuttle box with electric shock function - Google Patents

Experiment shuttle box with electric shock function Download PDF

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Publication number
CN2917269Y
CN2917269Y CN 200620018965 CN200620018965U CN2917269Y CN 2917269 Y CN2917269 Y CN 2917269Y CN 200620018965 CN200620018965 CN 200620018965 CN 200620018965 U CN200620018965 U CN 200620018965U CN 2917269 Y CN2917269 Y CN 2917269Y
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China
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shuttle box
rat
electric shock
experiment
transformer
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Expired - Fee Related
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CN 200620018965
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Chinese (zh)
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隋南
鞠平
杨彤
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Institute of Psychology of CAS
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Institute of Psychology of CAS
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Abstract

The utility model relates to a program-controlled training and detecting system of conditioned escaping and reflex which comprises: a mouse-crossing conjoined box separated by a clapboard in the middle, the clapboard is provided with a gate for mouse-crossing, the top of the mouse-crossing box is a top board made of transparent material, the boards at the two sides are provided with signal lamps and the bottom are provided with a shock plate which is made of metal column; the PC machine is an installed image collecting card, the video camera is mounted above the top board of the mouse-crossing box, the PC machine is electronically connected with the video camera as well as a displayer, a circuit controlling machine and controls a transformer, the shock plate the signal lamp. The utility model has the advantages of: convenient installment, training and detecting of more than one animal at the same time. The utility model can automatically complete the whole process of conditioned escaping and reflex of mouse with convenient operation and is labor-saving. The utility model can also detect and collect data with video camera with accurate and specified records and is increased with parameters of reflection period.

Description

A kind of experiment shuttle box with electric shock function
Technical field
The utility model relates to a kind of experimental provision that is used to detect the escape behavior that animal brought out by the signal or the electric shock of prompting electric shock, particularly relates to a kind of experiment shuttle box with electric shock function.
Background technology
The conditionity withdrawal reflex behavior model of animal has embodied study and the respond of animal to specific escape signal, is animal model commonly used in the research of neuropharmacology and Physiological Psychology, is widely used in medical science, in the experimental study in psychology field.In the experiment of training continuously, utilize shuttle box to carry out more.
Existing shuttle box is two chests placed side by side, and two casees sizes identical with interior (each case box plate is that row's metal is leant on, and on the sidewall signal lamp is arranged) communicate with a wicket between two the casees.When beginning training, the rat side that is placed in one is opened that side signal lamp of place, if after a period of time, rat still rests on puts into side, then gives certain intensity electric shock, and electric shock can make rat escape to offside.Rat enters the rear flank, can begin to begin to train from the offside case next time, and step is similar, and general one takes turns the repeatedly training that need carry out continuously.Wherein electric shock and optical signal can be by controls manually, and existing abroad device can use optoelectronic induction technology for detection rat position in shuttle box, and whole training process is controlled by computer program, but computer programming and application are western language, operation inconvenience and cost is higher, domestic do not see as yet produce the similar device report.Compare with manual training of early stage dependence experimenter,, can better control experiment condition by computer program control training detection down, more detailed and write down the performance of animal in experimentation accurately, and make things convenient for data preparation and statistics.
Summary of the invention
The purpose of this utility model is: provide a kind of can be in detail and write down the performance of animal in experimentation exactly, and can make things convenient for the programme control conditioned withdrawal reflex of data preparation and statistics to train and detection system, this system relies on shooting and image analysis technology to distinguish the movement locus of laboratory animal (rat), and trigger modulating signal and electric shock in the experimental box between in due course according to its movement locus, write down the motion conditions of rat in the whole experiment simultaneously, under the situation that parameters is set, automatically finish the training of conditionity reflection task, and the many index of behavior performance in the reflection animal withdrawal reflex experimentation is provided.
The purpose of this utility model is achieved in that
Programme control conditioned withdrawal reflex training and detection system (as Fig. 1) that the utility model provides comprising: PC 1, video frequency pick-up head 3 and rat shuttle box 2; It is characterized in that: also comprise image collection card 4, display 5, circuit controller 6 and circuit transformer 7; Wherein said rat shuttle box 2 is a middle conjuncted case that separates with dividing plate, have the door 10 that shuttles back and forth for rat on the dividing plate, the top of rat shuttle box 2 is the top board 9 that transparent material is made, difference signalization lamp 8 on the biside plate, and the bottom is provided with the electric-shocking plate of making of metal column 11; Described PC 1 is for installing image pick-up card 4, video frequency pick-up head 3 is arranged on the top of rat shuttle box top board 9, PC 1 is electrically connected with video frequency pick-up head 3, and PC 1 also is electrically connected with display 5, circuit controller 6 respectively, and transformer 7 is electrically connected and is subjected to it to control with electric-shocking plate 11, signal lamp 8.
In above-mentioned technical scheme, described image pick-up card 4 is SDK-2000 type image pick-up card or similar computer image pick-up card.
In above-mentioned technical scheme, described circuit controller 6 is the DYKZ-10 power supply switch controller.
In above-mentioned technical scheme, described transformer 7 adopts DH1719A type single channel current regulator power supply, and this power supply is a kind of with the constant voltage (CV) of 31/2 digital panel meter demonstration and the high-precision power of the automatic conversion of constant current (CC).
In above-mentioned technical scheme, described electric-shocking plate 11 is made metal cylinder for adopting iron or stainless steel, is arranged in parallel or netted arrangement is installed in the bottom of rat shuttle box 2, goes back serial resistance in rat shuttle box 2 outsides and is electrically connected with transformer 7.
In sum, the rat motor information that this device can utilize camera to obtain triggers signal lamp and electric-shocking plate in the shuttle box, finishes automatically the every motion index of animal at training or test phase trained and exported to the conditionity withdrawal reflex of animal.
Advantage of the present utility model is:
Programme control conditioned withdrawal reflex training of the present utility model and detection system are made up of PC, image collection card, video frequency pick-up head and circuit controller etc., by computer program whole training process is controlled, and computer programming and application are Chinese, compare with manual training of early stage dependence experimenter, detect by the training under the computer program control, system features in convenient, can better control experiment condition again, more detailed and write down the performance of animal in experimentation accurately, and make things convenient for data preparation and statistics, and system cost is low.
The method of utilizing this system that rat is trained and detects can be finished the whole training process of rat conditionity withdrawal reflex automatically under programme-control, easy to operate, saves manpower; Utilize camera monitoring and image data, write down detailed accurately, and parameter such as increase when reacting; The device simple installation can carry out many animal trainings simultaneously; Also can finish simultaneously the passive withdrawal reflex training of rat.
Description of drawings
Fig. 1 is that programme control conditioned withdrawal reflex training of the present utility model and detection system are formed schematic diagram
Fig. 2 is a rat shuttle box structural representation of the present utility model
The drawing explanation:
PC-1 rat shuttle box-2 video frequency pick-up head-3
Image collection card-4 display-5 circuit controller-6
Circuit transformer-7 signal lamp-8 top board-9
Door-10 electric-shocking plates-11
Embodiment
Embodiment 1
With reference to figure 2, make a rat shuttle box 2, this rat shuttle box 2 is a conjuncted chest that separates with the pavilion plate, two the casees size is identical with interior, dividing plate has the door 1 that shuttles back and forth for rat, rat shuttle box 2 tops are the top board 9 that transparent poly (methyl methacrylate) plate is made, and sidewall is opaque, this rat shuttle box 2 wide 45cm, long 90cm, high 45cm; One signal lamp 8 is arranged on two lateral walls of rat shuttle box 2, box plate is row's metal column, the electric-shocking plate 11 made of iron prop or stainless steel cylinder for example, metal column is arranged in parallel or netted arrangement is installed in the bottom of rat shuttle box 2, can also be in the outside of rat shuttle box 2, serial resistance is installed in the circuit between signal lamp 8 and the transformer 7.
The hardware environment of present embodiment system requires: 1, PC 1 is at least Pentium (Pentium) 800MHz or CPU at the same level, 32M internal memory at least; 2, hard drive space 30M at least; Provide if 3 softwares are the forms with CD, calculator preferably uses at least 40 times of speed or above CD drive;
Performance requirement: gather monitor window size 320 * 240, gather monitor window color 24Bit, image pick-up card uses 640*480 resolution.Image pick-up card 4 and behavioral experiment software also are installed on the PC 1, and link to each other with shuttle box 2 with transformer 7 with circuit controller 6, therefore PC can pass through mounted image pick-up card 4 on the one hand, behavioral experiment software forms data with this movable information and handles and store, can follow on the other hand according to preset condition and rat movable information at that time, immediately, trigger the modulating signal 8 and the electric-shocking plate 11 of shuttle box 2, start or the termination experiment by control to circuit controller 6 and transformer 7.
Video frequency pick-up head 3 is the WV-BP140 type, and its output video is black and white or colour.This video frequency pick-up head 3 is electrically connected with PC 1, can see through the motion (can monitor the rat that has density bullet under white rat under the non-white background or the white background) of rat in the rat shuttle box 2 top detection cases, its movement locus of track record, and movable information reached PC 1, installed image pick-up card 4 on the PC 1, behavioral experiment software, and be electrically connected with shuttle box 2 with circuit controller 6 and transformer 7.
Image pick-up card 4 is SDK-2000 (day quick exploitation-2000 type), and this image pick-up card 4 is supported Video ForWindows, and the image of dynamic acquisition is supported 24Bit 320 * 240 resolution.Systems soft ware environmental requirement: Windows95, Windwos97, Windows98, Windows2000, Windows Xp.
Display 5 adopts the TCL MF708T type CRT monitor of buying on the market, and this display 5 links to each other with PC, shows the instant image of rat motor and operation interface is provided.
Circuit controller 6 is the DYKZ-10 power supply switch controller, links to each other with PC with telephone wire for being installed by this circuit controller 6 of the gauge tap that expands of computer software control, and is connected to transformer 7.
Transformer 7 adopts DH1719A type single channel current regulator power supply, and this power supply is a kind of with the constant voltage (CV) of 31/2 digital panel meter demonstration and the high-precision power of the automatic conversion of constant current (CC).Can select to show output voltage and output current by the switch on the panel.
With reference to Fig. 1, make programme control conditioned withdrawal reflex training and detection system, modulating signal 8, electric-shocking plate 11, circuit controller 6 and the transformer 7 of the above-mentioned rat shuttle box made from reference to Fig. 22 is electrically connected, and is subjected to the control of PC 1; Video frequency pick-up head 3 is arranged on the top of rat shuttle box top board 9, and PC 1 is electrically connected with video frequency pick-up head 3, and PC 1 also is electrically connected and is subjected to it to control with display 5.
Because rat shuttle box 2 tops are transparent poly (methyl methacrylate) plate, the conditionity withdrawal reflex training of experimental rat is promptly carried out in this case, camera 3 is equipped with in rat shuttle box 2 tops (top, laboratory), this camera is connected in PC1, the motion that can see through rat in the rat shuttle box 2 top detection cases (can be monitored white rat under the non-white background, the rat that perhaps has density bullet under the white background), its movement locus of track record, and movable information reached PC, installed image pick-up card 4 on the PC, behavioral experiment software, and link to each other with rat shuttle box 2 with transformer 7 with circuit controller 6, therefore PC can pass through mounted image pick-up card 4 on the one hand, behavioral experiment software forms data with this movable information and handles and store, can follow on the other hand according to preset condition and rat movable information at that time, immediately, trigger the modulating signal and the electric shock of shuttle box, start or the termination experiment by control to circuit controller 6 and transformer 7.Display 5 shows the instant image of rat motor and operation interface is provided.
In programme control conditioned withdrawal reflex training and detection system that embodiment 1 makes, conditionity withdrawal reflex training of the rat that experimentizes and the method that detects place ground, laboratory with rat shuttle box 2 earlier:
A rat conditionity withdrawal reflex training method
Before the experiment beginning, regulate indoor light, making it to be fit to the interior animal of camera monitoring shuttle box moves, in the parameter that each time training is set on the PC, comprise promptly in each training that the side case that enters rat shuttle box 2 from rat opens to electric-shocking plate 11 and gives in the time T b=10s of electric shock, the every wheel group of the number of times 30 of training, rat numbering situation continuously to beginning the time T a=30 ± 5s to modulating signal, the each training signal lamp on the menu that program provided; The voltage strength that wherein shocks by electricity is a 20v voltage;
When beginning training, the experimenter places a side of rat shuttle box 2 with animal, and PC 1 can detect rat immediately and begin training by video frequency pick-up head 3; PC 1 is monitoring rat after the homonymy time of staying stops overtime 30s (Ta), even that sidewall signal lamp of place rat shuttle box 2 is opened, if open after during 10s (Tb) at lamp, rat still rests on that side of putting into, then give 20v electric shock for this side electric-shocking plate 11 by circuit controller 6 output electric pulses, electric shock can make rat escape to the offside of rat shuttle box 2.From signal lamp open to rat escape to the offside of rat shuttle box 2 for training once, rat enter rat shuttle box 2 to the rear flank, the offside case from rat shuttle box 2 under PC 1 monitoring and control begins to train next time, step is similar.If animal then is designated as once mistake, and begins to train from offside training interval (before the homonymy signal lamp is opened) to run offside next time, carry out 30 training back systems continuously and stop training and warning automatically.
The horizontal movement track of rat in the PC 1 record whole experiment, and provide the numerical value of multinomial reflection rat task performance through Data Management Analysis, with the form output of txt or excel, wherein display shows that the output data comprise:
A, escape time: the back animal occurs at modulating signal and putting into the time that side stops, Tc<Tb;
B, escape speed: the back animal occurs in the speed of putting into side shifting or distance at modulating signal;
When c, reaction: occur to the time that animal begins displacement from modulating signal;
D, hide the time: animal occurs behind time T b at modulating signal and still rest on when putting into side, shock by electricity to animal, putting into the time that side stops for electric shock back animal, resting on the total time of putting into side with animal after modulating signal occurs is T, then hides time=T-Tb;
E, hide number of times: the number of times that takes place at whole training process avoidance behaviour.(after the modulating signal appearance, in limiting time, not escaping number of times) to offside;
The movement velocity of f, training interval animal (time, distance);
G, interval the number of times that shuttles back and forth: one takes turns in the training, and animal runs the number of times of offside in training interval (before the homonymy signal lamp is opened).
The passive escape training of B rat
During parameter during the experiment beginning is provided with, signal lamp at every turn training is opened to base plate be made as 0s for the time (Tb) of electric shock, make modulating signal no longer have suggesting effect to electric shock, surplus step is the same.

Claims (6)

1. the experiment shuttle box with electric shock function comprises: PC (1), video frequency pick-up head (3) and rat shuttle box (2); It is characterized in that: also comprise image collection card (4), display (5), circuit controller (6) and circuit transformer (7); Wherein said rat shuttle box (2) is a middle conjuncted case that separates with dividing plate, have the door (10) that shuttles back and forth for rat on the dividing plate, the top of rat shuttle box (2) is the top board (9) that transparent material is made, difference signalization lamp (8) on the biside plate, the bottom is provided with the electric-shocking plate of making of metal column (11); Described PC (1) is equipped with image pick-up card (4), video frequency pick-up head (3) is arranged on the top of rat shuttle box top board (9), PC (1) is electrically connected with video frequency pick-up head (3), PC (1) also is electrically connected with display (5), circuit controller (6) respectively, and transformer (7) is electrically connected and is subjected to it to control with electric-shocking plate (11), signal lamp (8).
2. according to the described experiment shuttle box with electric shock function of claim 1, it is characterized in that: described image pick-up card (4) is a SDK-2000 type image pick-up card.
3. according to the described experiment shuttle box with electric shock function of claim 1, it is characterized in that: described circuit controller (6) is the DYKZ-10 power supply switch controller.
4. according to the described experiment shuttle box of claim 1 with electric shock function, it is characterized in that: described transformer (7) adopts DH1719A type single channel current regulator power supply, and this power supply is a kind of with the constant voltage of 31/2 digital panel meter demonstration and the power supply of the automatic conversion of constant current.
5. according to the described experiment shuttle box with electric shock function of claim 1, it is characterized in that: described electric-shocking plate (11) is for adopting stainless steel metal cylinder, is arranged in parallel or netted arrangement is installed in the bottom of rat shuttle box (2).
6. according to the described experiment shuttle box of claim 1, it is characterized in that: also be included in serial resistance between the circuit of the signal lamp (8) of rat shuttle box (2) and transformer (7) with electric shock function.
CN 200620018965 2006-04-04 2006-04-04 Experiment shuttle box with electric shock function Expired - Fee Related CN2917269Y (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388814A (en) * 2011-09-01 2012-03-28 上海移数信息科技有限公司 Intelligent complex shuttle control analysis system
CN102499109A (en) * 2011-10-20 2012-06-20 上海移数信息科技有限公司 High-grade intelligent scene fear analysis system
CN102511406A (en) * 2011-10-20 2012-06-27 上海移数信息科技有限公司 High-grade full-automatic intelligent complicated bar rotating instrument
CN104523232A (en) * 2014-12-26 2015-04-22 中国科学院深圳先进技术研究院 Detecting system for small animal fear behaviors
CN105454091A (en) * 2016-01-14 2016-04-06 中国科学院合肥物质科学研究院 Experimental device for researching behavioral characteristics of bee under electrical stimulation
CN106137170A (en) * 2016-08-10 2016-11-23 深圳先进技术研究院 A kind of intelligent foot shock system based on biofeedback
CN109350083A (en) * 2018-11-20 2019-02-19 遵义医学院 A kind of preference case of changeable colour
CN114223574A (en) * 2021-12-16 2022-03-25 中国科学院深圳先进技术研究院 Animal selective behavior output detection device and experimental method

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388814A (en) * 2011-09-01 2012-03-28 上海移数信息科技有限公司 Intelligent complex shuttle control analysis system
CN102388814B (en) * 2011-09-01 2014-10-08 上海移数信息科技有限公司 intelligent complex shuttle control analysis system
CN102499109A (en) * 2011-10-20 2012-06-20 上海移数信息科技有限公司 High-grade intelligent scene fear analysis system
CN102511406A (en) * 2011-10-20 2012-06-27 上海移数信息科技有限公司 High-grade full-automatic intelligent complicated bar rotating instrument
CN104523232A (en) * 2014-12-26 2015-04-22 中国科学院深圳先进技术研究院 Detecting system for small animal fear behaviors
CN104523232B (en) * 2014-12-26 2018-07-06 中国科学院深圳先进技术研究院 A kind of meiofauna fear behavioral value system
CN105454091A (en) * 2016-01-14 2016-04-06 中国科学院合肥物质科学研究院 Experimental device for researching behavioral characteristics of bee under electrical stimulation
CN106137170A (en) * 2016-08-10 2016-11-23 深圳先进技术研究院 A kind of intelligent foot shock system based on biofeedback
CN109350083A (en) * 2018-11-20 2019-02-19 遵义医学院 A kind of preference case of changeable colour
CN109350083B (en) * 2018-11-20 2023-11-03 遵义医学院 Color-changeable preference box
CN114223574A (en) * 2021-12-16 2022-03-25 中国科学院深圳先进技术研究院 Animal selective behavior output detection device and experimental method

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C17 Cessation of patent right
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Granted publication date: 20070704

Termination date: 20110404