CN2906602Y - High-throughput gene and protein analyzer - Google Patents
High-throughput gene and protein analyzer Download PDFInfo
- Publication number
- CN2906602Y CN2906602Y CN 200620079036 CN200620079036U CN2906602Y CN 2906602 Y CN2906602 Y CN 2906602Y CN 200620079036 CN200620079036 CN 200620079036 CN 200620079036 U CN200620079036 U CN 200620079036U CN 2906602 Y CN2906602 Y CN 2906602Y
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- filter set
- polarizer
- exciting light
- light filter
- semi
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a gene biotechnology area, in particular to an analyzer for high-throughput gene and protein. The utility model overcomes the inaccurate test result which exits in the existing technology which has the disadvantages of the incapability for analyzing the gene structure and the incapability for analyzing and testing the protein of the pathogenic microorganisms. The utility model is composed of an excited optical filter group, an excitation light source, a photomultiplier and a sample disc, and also comprises a launch optical filter unit, a semi-lens reflex, an inspire optical polarizer, a launch light polarizer and a liquid fiber; the semi-lens reflex is tipsily arranged on the top of the measurement of the sample disc, and the inspire optical polarizer is vertically arranged at the vertical direction of the sample disc, and the luminous direction of the excitation light source is towards the excited optical filter group, and the liquid fiber is arranged between the excited optical filter group and the inspire optical polarizer; the top of the semi-lens reflex is provided with a launch optical polarizer, a launch optical filter group and a photomultiplier.
Description
Affiliated technical field:
The utility model relates to the gene biological technical field, is specifically related to a kind of high flux gene, protein analyzer.
Background technology:
High flux gene, protein analyzer adopt the method for fluorescence polarization, can carry out Genotyping fast and detect and genetic analysis, and the analysis of protein of pathogenic microorganism detects.Existing fluorescence detector on the market can detect the fluorescence intensity level of different wave length, can be applied to the fluorescent reagent box and detect, and its major function is the genetic test of pathogenic microorganism.But the weak point that fluorescence detector exists is: fluorescence intensity detects the interference that is subjected to fluorescence background makes the result be easy to generate false positive, causes testing result inaccurate; Can only be used for genetic test on the function, can not analyze gene structure, the analysis of protein that can not satisfy the pathogenic microorganism that generally uses clinically detects, thereby has limited its application in clinical detection.
Summary of the invention:
The utility model will provide a kind of high flux gene, protein analyzer, and is inaccurate to overcome the testing result that prior art exists, and can not analyze the shortcoming that can not detect the analysis of protein of pathogenic microorganism to gene structure.
For overcoming the deficiency that prior art exists, the technical solution of the utility model is: a kind of high flux gene, protein analyzer, comprise exciting light filter set 1, excitation source 2, photomultiplier 3 and sample disc 5, its special character is: also comprise emission light filter set 4, reflection semi-permeable mirror 6, the exciting light polarizer 7, emission light polarization plate 8 and liquid fiber 9, the top, measurement position of described sample disc 5 is provided with the reflection semi-permeable mirror 6 that is obliquely installed, be vertically installed with the exciting light polarizer 7 on its reflecting surface direction, the light emission direction of excitation source 2 is provided with liquid fiber 9 towards exciting light filter set 1 between the exciting light filter set 1 and the exciting light polarizer 7; Be disposed with emission light polarization plate 8, emission light filter set 4 and photomultiplier 3 directly over the described reflection semi-permeable mirror 6.
Above-mentioned exciting light filter set 1 is rotatable discoid.Be convenient to adjust selection.
But above-mentioned emission light filter set 4 is the tabular of horizontal anomalous movement.
Above-mentioned excitation source 2 is selected the halogen tungsten lamp of wavelength at 340-700nm for use.
Compared with prior art, the utility model has the advantages that:
1, clinical detection is widely used: the utility model not only can detect gene signal, more remedied the deficiency of fluorescence detector, avoided interference, can analyze, also be applicable to that pathogenic microorganism detects and genetic analysis simultaneously dna structure to tested signal.
2, it is highly sensitive, good measuring accuracy: the principle of this equipment utilization fluorescence polarization, with photomultiplier (PMT), detect the polarized light intensity of parallel direction light intensity and vertical direction, thereby calculate the polarization value (mP of polarization value unit) of sample.Wherein photomultiplier can amplify signal, improves detection sensitivity, and the optical filtering of multiple optical filter can improve the precision of detection.
3, operating automation degree height: computer control, can analyze the gene target spot, owing in testing process, do not need to wash operations such as plate, replacing sample, use the utility model to simplify the step of genetic analysis greatly, simple to operate, detect fast, but 384 samples of continuous detecting once.
Description of drawings:
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is the structural representation of exciting light filter set;
Fig. 3 is the structural representation of emission light filter set.
Description of reference numerals is as follows:
1-exciting light filter set, the 2-excitation source, the 3-photomultiplier, 4-emission light filter set, the 5-sample disc, 6-reflects semi-permeable mirror, the 7-exciting light polarizer, 8-reflected light polaroid, 9-liquid fiber.
Embodiment:
Below in conjunction with drawings and Examples the utility model is elaborated.
Referring to Fig. 1～Fig. 3, a kind of high flux gene, protein analyzer comprise exciting light filter set 1, excitation source 2, photomultiplier 3, emission light filter set 4, sample disc 5, reflection semi-permeable mirror 6, the exciting light polarizer 7, emission light polarization plate 8 and liquid fiber 9.The detecting position top of said sample disc 5 is provided with the reflection semi-permeable mirror 6 that is obliquely installed, be vertically installed with the exciting light polarizer 7 on its reflecting surface direction, said excitation source 2 is the halogen tungsten lamp of wavelength at 340-700nm, its light emission direction is towards discoid exciting light filter set 1, this exciting light filter set 1 can be rotated in vertical direction, is provided with liquid fiber 9 between the exciting light filter set 1 and the exciting light polarizer 7.Be disposed with emission light polarization plate 8, emission light filter set 4 and photomultiplier 3 directly over the said reflection semi-permeable mirror 6, but said emission light filter set 4 is the tabular of horizontal anomalous movement.
The principle of fluorescence polarization of utilizing of the present utility model is carried out work.Halogen tungsten lamp becomes the light of specific wavelength behind an optical filter on the exciting light filter set 1 as the light of the light source generation of wave spectrum continuously.This Shu Guangjing liquid fiber 9 transmits again through 7 of the exciting light polarizers inclined to one side (polarization direction is perpendicular to exciting light), and the light of filtration is refracted on the sample in the sample disc 5 the emission light that fluorescent dye is excited and sends different wave length in the sample through reflecting semi-permeable mirror 6.Radiative a part of penetration semi-permeable mirror 6, send into photomultiplier 3 behind the optical filter in emission light filter set 4 again after (polarization direction is parallel to emission light) partially through 8 of light polarization plates of emission, obtain the polarization value (mP of polarization value unit) that device software behind the polarized light intensity of parallel direction light intensity and vertical direction can calculate sample automatically according to following formula.
The inversely proportional relation of fluorophor molecular motion speed in the size of fluorescence polarization numerical value and the sample, and the inversely proportional relation of molecular motion speed and bulk of molecule.What material to be detected can be special in the experiment combines with fluorescent tag molecule, when containing this detection material in the sample, fluorescently-labeled molecule combines with material to be detected, the fluorescence polarization value height of the fluorescent tag molecule of its fluorescence polarization value that demonstrates meeting specific ionization.Therefore by to the reading of final detected fluorescence polarization value, can judge whether contain material to be detected and the corresponding gene information of this detection material in institute's test sample.
Claims (4)
1, a kind of high flux gene, protein analyzer, comprise exciting light filter set (1), excitation source (2), photomultiplier (3) and sample disc (5), it is characterized in that: also comprise emission light filter set (4), reflection semi-permeable mirror (6), the exciting light polarizer (7), emission light polarization plate (8) and liquid fiber (9), the top, measurement position of described sample disc (5) is provided with the reflection semi-permeable mirror (6) that is obliquely installed, be vertically installed with the exciting light polarizer (7) on its reflecting surface direction, the light emission direction of excitation source (2) is provided with liquid fiber (9) towards exciting light filter set (1) between the exciting light filter set (1) and the exciting light polarizer (7); Be disposed with emission light polarization plate (8), emission light filter set (4) and photomultiplier (3) directly over the described reflection semi-permeable mirror (6).
2, high flux gene according to claim 1, protein analyzer is characterized in that: described exciting light filter set (1) is for rotatable discoid.
3, high flux gene according to claim 1 and 2, protein analyzer is characterized in that: described emission light filter set (4) but be the tabular of horizontal anomalous movement.
4, high flux gene according to claim 3, protein analyzer is characterized in that: described excitation source (2) is selected the halogen tungsten lamp of wavelength at 340-700nm for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620079036 CN2906602Y (en) | 2006-05-24 | 2006-05-24 | High-throughput gene and protein analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620079036 CN2906602Y (en) | 2006-05-24 | 2006-05-24 | High-throughput gene and protein analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2906602Y true CN2906602Y (en) | 2007-05-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620079036 Expired - Fee Related CN2906602Y (en) | 2006-05-24 | 2006-05-24 | High-throughput gene and protein analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2906602Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102410473A (en) * | 2011-08-26 | 2012-04-11 | 深圳市锦瑞电子有限公司 | Light source, protein analyzer containing same and protein analytical method |
-
2006
- 2006-05-24 CN CN 200620079036 patent/CN2906602Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102410473A (en) * | 2011-08-26 | 2012-04-11 | 深圳市锦瑞电子有限公司 | Light source, protein analyzer containing same and protein analytical method |
| CN102410473B (en) * | 2011-08-26 | 2013-09-25 | 深圳市锦瑞电子有限公司 | Light source, protein analyzer containing same and protein analytical method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070530 Termination date: 20150524 |
|
| EXPY | Termination of patent right or utility model |