CN102410473A - Light source, protein analyzer containing same and protein analytical method - Google Patents
Light source, protein analyzer containing same and protein analytical method Download PDFInfo
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- CN102410473A CN102410473A CN201110249406XA CN201110249406A CN102410473A CN 102410473 A CN102410473 A CN 102410473A CN 201110249406X A CN201110249406X A CN 201110249406XA CN 201110249406 A CN201110249406 A CN 201110249406A CN 102410473 A CN102410473 A CN 102410473A
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Abstract
The invention provides a light source which comprises a tube body, an LED (light emitting diode) and a lens, wherein the tube body is provided with an open end, the LED is accommodated in the tube body, and the lens is fixed on the open end and is arranged in the light emergent direction of the LED; the light source adopts the LED as a light-emitting source, the LED emits parallel lights, the parallel lights can be focused by the lens so as to form a light beam with better stability, and the light source is small in size. The invention also provides a protein analyzer containing the light source and a protein analytical method.
Description
[technical field]
The present invention relates to a kind of fluid analyte meter, particularly relate to a kind of light source, contain the protein analyzer and the analysis of protein method of this light source.
[background technology]
Protein analyzer is used for gene, granular size to protein etc. and analyzes.Present protein analyzer generally adopts iodate silicon wafer bulb, halogen tungsten lamp as the light source of measuring light emission.For example, Chinese patent CN200620079036 discloses a kind of protein analyzer.Said protein analyzer comprises exciting light filter set, halogen tungsten lamp, photomultiplier, emission light filter set, sample disc, reflection pellicle mirror, the exciting light polarizer, emission light polarization plate and liquid fiber.The detecting position top of sample disc is tiltedly installed with the reflection pellicle mirror, is vertically installed with the exciting light polarizer on its reflecting surface direction.The light emission direction of halogen tungsten lamp is towards discoid exciting light filter set, and said exciting light filter set can be rotated in vertical direction, between the exciting light filter set and the exciting light polarizer, is provided with liquid fiber.Be disposed with emission light polarization plate, emission light filter set and photomultiplier directly over the reflection pellicle mirror, but said emission light filter set is the tabular of horizontal anomalous movement.
Halogen tungsten lamp is as the light source of continuous wave spectrum, and the light of generation becomes the light of specific wavelength behind an optical filter on the exciting light filter set.This Shu Guangjing liquid fiber transmission plays (polarization direction is perpendicular to exciting light) partially through the exciting light polarizer again, and the light of filtration is refracted on the sample in the sample disc emission light that fluorescent dye is excited and sends different wave length in the sample through the reflection pellicle mirror.Radiative a part of penetration pellicle mirror;, the emission light polarization plate sends into photomultiplier behind the optical filter in emission light filter set again after playing partially (polarization direction is parallel to emission light); Device software calculates the polarization value (mP of polarization value unit) of sample after obtaining the polarized light intensity of parallel direction light intensity and vertical direction, thereby the gene of testing protein is analyzed.
Yet the light source of above-mentioned protein analyzer is a halogen tungsten lamp, and its emitted light beams is unstable, and volume is bigger.
[summary of the invention]
In view of above-mentioned condition, be necessary to provide the light source that a kind of volume is less and light beam is stable.
A kind of light source comprises
Body has an openend;
LED is contained in the said body; And
Lens are fixed in said openend and are positioned at said LED light direction.
Further, said lens are convex lens.
Further, the focal length of said lens is 20 centimetres.
Simultaneously, the present invention also provides a kind of protein analyzer and analysis of protein method that contains above-mentioned light source.
A kind of protein analyzer comprises:
Above-mentioned light source; And
First grating is arranged at the beam emissions direction of said light source.
Further, said protein analyzer also comprises with said first grating and is provided with, is positioned at second grating of more said first grating of beam emissions direction of said light source away from said light source position at interval.
Further, the spacing of said first grating and said second grating is 115 millimeters.
Further, the grating diameter of said first grating and second grating is 2.5 millimeters.
A kind of analysis of protein method comprises the steps:
A branch of collimated light beam is provided;
Said collimated light beam is focused on;
Collimated light beam after focusing on is carried out wavelength to be filtered; And
Collimated light beam after filtering is shone on the transparent vessel that testing protein liquid is housed.
Further, said analysis of protein method comprises that also the collimated light beam after filtering is carried out wavelength once more to be filtered.
Further, said analysis of protein method also comprises the concentration height of judging said testing protein liquid according to said collimated light beam through the scattered light intensity behind the said transparent vessel.
The light source of above-mentioned protein analyzer adopts LED to make illuminating source, and LED launches directional light, after focusing on through lens, form stability light beam preferably, and the light source volume is less.
[description of drawings]
Fig. 1 is the sketch map of the protein analyzer of this embodiment;
Fig. 2 is the flow chart of the analysis of protein method of this embodiment.
[specific embodiment]
For the ease of understanding the present invention, will more comprehensively describe the present invention with reference to relevant drawings below.Provided preferred embodiment of the present invention in the accompanying drawing.But the present invention can realize with many different forms, be not limited to embodiment described herein.On the contrary, provide the purpose of these embodiment be make the understanding of disclosure of the present invention comprehensively thorough more.
Need to prove, when element is called as " being fixed in " another element, it can be directly on another element or also can have element placed in the middle.When an element is considered to " connection " another element, it can be to be directly connected to another element or possibly to have element placed in the middle simultaneously.Term as used herein " vertical ", " level ", " left side ", " right side " and similar statement are just for illustrative purposes.
Only if definition is arranged in addition, the employed all technology of this paper are identical with the implication that belongs to those skilled in the art's common sense of the present invention with scientific terminology.Among this paper in specification of the present invention employed term be not intended to be restriction the present invention just in order to describe the purpose of concrete embodiment.Term as used herein " and/or " comprise one or more relevant Listed Items arbitrarily with all combinations.
See also Fig. 1, the protein analyzer 100 of this embodiment comprises light source 110, first grating 120, second grating 130 and transparent vessel 140.
Light source 110 comprises body 111, LED 112, lens 113 and power governor 114.Body 111 is roughly cylindrical shape, has an openend (figure is mark not).LED 112 is contained in the body 111.LED can be one, also can be a plurality of.Lens 113 are fixed in openend, and are positioned at LED 112 light directions.Lens 113 can be the set of lenses of convex lens, convex lens and concave and convex lenses composition etc.Specifically in this embodiment, lens 113 are convex lens.The focal length of lens 113 is 20 centimetres.Power governor 114 is contained in the body 111 and with LED 112 and is electrically connected, and is used to regulate the luminous power of LED 112.Specifically in this embodiment, the power of light source 110 is 1 milliwatt.
Need to prove that power governor 114 also can omit, at this moment, the power of light source 110 is firm power.
First grating 120 is arranged at the beam emissions direction of light source 110.The beam emissions direction that second grating 130 and first grating 120 were provided with, were positioned at light source 110 at interval is than the position of first grating 120 away from light source.Specifically in this embodiment, the spacing of first grating 120 and second grating 130 is 115 millimeters.The grating diameter of first grating 120 and second grating 130 is 2.5 millimeters.
Be appreciated that second grating 130 also can omit, at this moment, the light beam that light source 110 sends filters metapedes through first grating and gets final product to satisfy test condition.
Transparent vessel 140 can be light transmission glass etc. preferably.Transparent vessel 140 is loaded with testing protein liquid, and testing protein liquid is the molecule liquid reagent mixing material in protein and the reaction tank to be tested, and antigen-antibody forms colloid through reaction.
The light source 110 of above-mentioned protein analyzer 100 adopts LED 112 to make illuminating source, and LED 112 launches directional light, after focusing on through lens 113, form stability light beam preferably, and light source 110 volumes is less.
And the light source 110 of above-mentioned protein analyzer 100 and first grating 120 and second grating 130 can be formed the Laser emission and the detection optical system of a sealing.Light source 110 can get up LED 112, lens 113, power governor 114 encapsulation through semiconductor packaging process with body 111.
Protein analyzer 100 also comprises mechanism for testing (figure does not show).The transmitting terminal of mechanism for testing is provided with orthogonal twin shaft, and twin shaft is fixed in mechanism for testing with light source 110, to install, to dismantle light source 110 easily, is prone to the purpose that dress is prone to accent thereby reach.
See also Fig. 2, the present invention also provides a kind of analysis of protein method, comprises step S201～S206.
Step S201 provides a collimated light beam.Specifically in this embodiment, the LED 112 of the light source 110 of protein analyzer 100 launches a collimated light beam.
Need to prove that " collimated light beam " in the present patent application is meant light direction light beam preferably, is to be equivalent to disperse light source, is not meant the completely parallel light beam in the ideal.
Step S202 focuses on collimated light beam.Specifically in this embodiment, the LED 112 of the light source 110 of protein analyzer 100 launches a collimated light beam, converges through behind the lens 113.
Step S203 carries out wavelength with the collimated light beam after focusing on and filters.Specifically in this embodiment, after light source 110 emitted light beams of protein analyzer 100 see through first grating 120, carry out wavelength and filter.
Step S204 carries out wavelength once more with the collimated light beam after filtering and filters.Specifically in this embodiment, after light source 110 emitted light beams of protein analyzer 100 see through second grating 130, carry out wavelength once more and filter.
Step S205 shines the collimated light beam after filtering on the transparent vessel that protein liquid to be measured is housed.Specifically in this embodiment, the light beam irradiates that sees through second grating 130 is to the transparent vessel that is loaded with testing protein liquid 140.
Step S206 is according to the concentration height of collimated light beam through the judgement of the scattered light intensity behind transparent vessel testing protein liquid.Be molecule liquid reagent hybrid reaction in protein and the reaction tank, antigen-antibody forms colloid through reaction, according to the Tyndall effect, when light beam passes through coarse disperse system, because particle greater than the incident light wavelength, mainly reflects; When light beam passes through colloidal solution, because colloid diameter less than visible wavelength, scattering takes place mainly; When light beam passes through molecular solution, because solution very evenly, scattered light is offset because of mutual interference fully.According to this principle, vertically to inject in the transparent vessel 140 through the light beam of second grating 130, the difference of colloid concentration is with the difference of corresponding scattered light intensity in the liquid of transparent vessel 140, and concentration is high more, and scattered light is strong more.
Be appreciated that above-mentioned steps S204 also can omit.
The above embodiment has only expressed several kinds of embodiments of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art under the prerequisite that does not break away from the present invention's design, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Claims (10)
1. a light source is characterized in that, comprises
Body has an openend;
LED is contained in the said body; And
Lens are fixed in said openend and are positioned at said LED light direction.
2. light source as claimed in claim 1 is characterized in that, said lens are convex lens.
3. light source as claimed in claim 2 is characterized in that, the focal length of said lens is 20 centimetres.
4. a protein analyzer is characterized in that, comprising:
Like each described light source of claim 1～3; And
First grating is arranged at the beam emissions direction of said light source.
5. protein analyzer as claimed in claim 4 is characterized in that, said protein analyzer also comprises with said first grating and is provided with, is positioned at second grating of more said first grating of beam emissions direction of said light source away from said light source position at interval.
6. protein analyzer as claimed in claim 5 is characterized in that, the spacing of said first grating and said second grating is 115 millimeters.
7. protein analyzer as claimed in claim 5 is characterized in that, the grating diameter of said first grating and second grating is 2.5 millimeters.
8. an analysis of protein method comprises the steps:
A branch of collimated light beam is provided;
Said collimated light beam is focused on;
Collimated light beam after focusing on is carried out wavelength to be filtered; And
Collimated light beam after filtering is shone on the transparent vessel that testing protein liquid is housed.
9. analysis of protein method as claimed in claim 8 is characterized in that, said analysis of protein method comprises that also the collimated light beam after filtering is carried out wavelength once more to be filtered.
10. analysis of protein method as claimed in claim 8 is characterized in that, said analysis of protein method also comprises the concentration height of judging said testing protein liquid according to said collimated light beam through the scattered light intensity behind the said transparent vessel.
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| CN 201110249406 CN102410473B (en) | 2011-08-26 | 2011-08-26 | Light source, protein analyzer containing same and protein analytical method |
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| CN 201110249406 CN102410473B (en) | 2011-08-26 | 2011-08-26 | Light source, protein analyzer containing same and protein analytical method |
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| CN102410473A true CN102410473A (en) | 2012-04-11 |
| CN102410473B CN102410473B (en) | 2013-09-25 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2906602Y (en) * | 2006-05-24 | 2007-05-30 | 陕西阳光生物工程股份有限公司 | High-throughput gene and protein analyzer |
| CN101000298A (en) * | 2006-12-22 | 2007-07-18 | 吉林大学 | Investigator based on optical fibre raster |
| CN101419156A (en) * | 2007-10-23 | 2009-04-29 | 深圳迈瑞生物医疗电子股份有限公司 | Spectrophotometric detecting method and apparatus , and detecting system |
| CN201354960Y (en) * | 2009-01-21 | 2009-12-02 | 简文进 | Light emitting diode lamp cap structure |
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2011
- 2011-08-26 CN CN 201110249406 patent/CN102410473B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2906602Y (en) * | 2006-05-24 | 2007-05-30 | 陕西阳光生物工程股份有限公司 | High-throughput gene and protein analyzer |
| CN101000298A (en) * | 2006-12-22 | 2007-07-18 | 吉林大学 | Investigator based on optical fibre raster |
| CN101419156A (en) * | 2007-10-23 | 2009-04-29 | 深圳迈瑞生物医疗电子股份有限公司 | Spectrophotometric detecting method and apparatus , and detecting system |
| CN201354960Y (en) * | 2009-01-21 | 2009-12-02 | 简文进 | Light emitting diode lamp cap structure |
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| CN102410473B (en) | 2013-09-25 |
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Address after: Nanshan District Taoyuan street 518000 Guangdong city of Shenzhen Province Liu Xian Da Dao Nan Shan Yungu Innovation Industrial Park 1183 building 6 floor B landscape Patentee after: SHENZHEN GENRUI BIOTECHNOLOGY CO., LTD. Address before: 518067, C, building 6, Shekou seven industrial road, Shekou, Guangdong, Nanshan District, Shenzhen Patentee before: Shenzhen Genius Electronics Co., Ltd. |
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Application publication date: 20120411 Assignee: Kangtai medical system (Qinhuangdao) Limited by Share Ltd Assignor: SHENZHEN GENRUI BIOTECHNOLOGY CO., LTD. Contract record no.: 2019440020001 Denomination of invention: Light source, protein analyzer containing same and protein analytical method Granted publication date: 20130925 License type: Common License Record date: 20190109 |
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Address after: 518000 B, 6th floor, Shanshui building, Nanshan cloud Valley Innovation Industrial Park, 1183 Liuxian Avenue, Taoyuan Street, Nanshan District, Shenzhen City, Guangdong Province Patentee after: Shenzhen Jinrui Biotechnology Co.,Ltd. Address before: 518000 B, 6th floor, Shanshui building, Nanshan cloud Valley Innovation Industrial Park, 1183 Liuxian Avenue, Taoyuan Street, Nanshan District, Shenzhen City, Guangdong Province Patentee before: GENRUI BIOTECH Inc. |