CN2893708Y - Terramycin collaurum method detection test paper - Google Patents
Terramycin collaurum method detection test paper Download PDFInfo
- Publication number
- CN2893708Y CN2893708Y CN 200620019378 CN200620019378U CN2893708Y CN 2893708 Y CN2893708 Y CN 2893708Y CN 200620019378 CN200620019378 CN 200620019378 CN 200620019378 U CN200620019378 U CN 200620019378U CN 2893708 Y CN2893708 Y CN 2893708Y
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- terramycin
- adsorptive pads
- test paper
- monoclonal antibody
- terromycin
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Abstract
A terramycin colloidal colloidal gold diagnostic strips, the utility model relates to a material in testing substances by reactions of marked antibodies and the antigen immune. The present utility model is made by fixing the terromycin coupled with the antibody, the anti-rat IgG antibody, the terromycin single antibody marked with the colloidal gold and other reagents on the cellulose nitrate film, adopting the principle of the chromatography type one-step competition method to detect the residual quantity of terromycin in the samples. The operation process of the present utility model takes not more than 20 minutes and the sensitivity is 2ppb, which is convenient and economical and can fulfill the rapid detection requirements of residual terromycin in samples of milk, serum, fodder, urine, meats, and tissue extractions.
Description
Affiliated technical field
The utility model belongs to labelled antibody and antigen immune and reacts the test paper of testing test substance.
Background technology
Terramycin (Oxytetracyclin) belongs to Cyclomycin family antibiotic, has broad spectrum antibiotic activity, it is cheap, therefore controls some animality disease, promotes growing of animal as the livestock and poultry feed adjuvant, is widely used in animal meat products production.Because its medicament residue of being caused of abuse has potential hazard to health, so its residual strictness control of limiting the quantity of and being subjected to the national Ministry of Agriculture in the animal flesh goods, terramycin meat products residual limiting the quantity of be 0.1mg/kg.At present, the method of measuring terramycin mainly contains methods such as high performance liquid chromatography (HPLC) and enzyme linked immunological, the HPLC method requires to have special instrument and equipment and professional and technical personnel, and sample screening amount less, need the pre-treatment of sample, therefore it is long that it detects cost height, detection time, these effects limit the HPLC method to the carrying out of terramycin test item, can not satisfy the needs that present food security detects the terramycin residual quantity fully.And the main dependence on import of ELISA reagent, it is higher to detect cost.
Summary of the invention
It is easy that the purpose of this utility model provides using method, time saving and energy saving saving money, and highly sensitive terramycin detects test paper.
The utility model Veritying test paper of oxytetracycline gel:
Comprise the nitrocellulose filter 6 that is bonded on the liner plate 7, it is characterized in that along nitrocellulose filter 6 vertical surfaces from bottom to top compartment of terrain set gold mark terramycin monoclonal antibody 2, terramycin coupled antigen 3 and anti-mouse IgG antibody 4 successively, constitute composite bed.
Connect with last adsorptive pads 5 in described nitrocellulose filter 6 upper ends; Lower end or be connected with following adsorptive pads 1 by gold mark terramycin monoclonal antibody 2, or directly connect with following adsorptive pads 1.
Described adsorptive pads 1 down, gold are marked terramycin monoclonal antibody 2 and are gone up adsorptive pads 5 surfaces and post self-adhesive paper.
Described test paper, is with as reference with anti-mouse IgG antibody 4 as detecting band with terramycin coupled antigen 3.
Gold mark terramycin monoclonal antibody and terramycin coupled antigen are the setting of lowest detection standard volume.
The utility model principle: the terramycin molecule in the sample liquid is moved to the film upper end by the effect of nitrocellulose filter capillary siphoning, and at gold mark monoclonal antibody place, the terramycin molecule combines with gold mark monoclonal antibody and forms compound; When the terramycin molecule was excessive in the sample liquid, compound floated to two anti-reference bands and is captured, and it is constant to detect the band color; When the terramycin molecule did not reach examination criteria, gold mark monoclonal antibody floated to detect to be with and combines with the terramycin coupled antigen, detects the band colour developing.Therefore, detect the band color along with terramycin molecule content size variation in the sample liquid.
The utility model whole operation process need only 20 minutes, and easy, quick, economy, sensitivity can reach 1ppb, samples such as milk, serum, feed, urine, meat and tissue extract are carried out the terramycin residual quantity detect, and can satisfy the requirement of fast detecting.
Description of drawings
Fig. 1 is the utility model front view.
Fig. 2 is that adsorptive pads is marked the side view that the terramycin monoclonal antibody partly overlaps with gold under the utility model.
Fig. 3 is the side view of adsorptive pads and cellulose nitrate film cascade under the utility model.
Embodiment
One, test paper
Comprise among the figure that following adsorptive pads 1, gold are marked terramycin monoclonal antibody 2, terramycin coupled antigen 3, anti-mouse IgG antibody 4, gone up adsorptive pads 5, nitrocellulose filter 6, plastics lining board 7.With gold mark terramycin monoclonal antibody 2, terramycin coupled antigen 3, sheep anti-mouse igg antibody 4 at interval 4mm spread in proper order base plate 7 middle parts nitrocellulose filter 6 on, form composite beds with nitrocellulose filter 6 surfaces.
Following adsorptive pads 1 and solid phase gold are marked monoclonal antibody 2 surfaces and are gone up adsorptive pads 5 surfaces and post colored self-adhesive paper.
The antibody titer of terramycin monoclonal antibody is 15000; Gold mark terramycin monoclonal antibody and terramycin coupled antigen are provided with for standard volume 1ppb.
Wherein,, be with as reference as detecting band with terramycin coupled antigen 3 with anti-mouse IgG antibody 4.
Film bar flat appearance, the material adhesion-tight, width 2.5mm, the liquid speed of dividing a word with a hyphen at the end of a line is not less than 5mm/min.
Fig. 2 is pasted on base plate 7 by gold mark terramycin monoclonal antibody 2 one ends with following adsorptive pads 1, and marks terramycin monoclonal antibody 2 parts overlapping with the gold on nitrocellulose filter 6 surfaces; Last adsorptive pads 5 is pasted on base plate 7 and is with 4 one ends by reference, and overlaps with nitrocellulose filter 6 one ends.
Fig. 3 is pasted on base plate 7 by gold mark terramycin monoclonal antibody 2 one ends with following adsorptive pads 1, and overlaps with nitrocellulose filter 6 parts; Last adsorptive pads 5 is pasted same Fig. 2.
This test paper is single solid bar formula structure and can installs plastics protection intermediate plate as required additional.
Two, test paper uses
(1) specimen preparation
Serum: extract animal blood to be checked, centrifugal or leave standstill after get transparent supernatant and use; If serum has excessive haemolysis, serum is experimentized with behind one times of the distilled water diluting again, otherwise the analoids redness is too dark, influences test result.
Urine: directly test with urine, as muddiness, the dilution in 1: 1 of first centrifuging and taking supernatant or distilled water.
Feed: get the feed adding 10ml dilution to be measured that 1g one grinds, therebetween every firmly concussion of 2min; During 10min, leave standstill 1min after shaking all and get supernatant 100 μ l, analyze with test paper after doing 10 times of dilutions with dilution.
(2) operation and detection
Test paper arrow end is immersed in the sample to be tested, does not surpass adsorptive pads down.Immerse the 30sec taking-up and set level, 10min reads the result.
Negative:
Two bands all develop the color, and detect and be with color to be deeper than with reference to the band color, do not contain terramycin in the interpret sample.
Two bands all develop the color, and detect the band color with identical or approaching with reference to the band color, and the terramycin content of interpret sample is lower than 1ppb (1ng/ml).
Positive:
Two bands all develop the color, and are shallower than with reference to the band color but detect the band color, illustrate that terramycin content surpasses 1ppb (1ng/ml) in the test sample.
Detect band and do not develop the color, with reference to the band colour developing, the terramycin content in the interpret sample is higher than 5ppb (5ng/ml).
Testing result is as shown in the table:
| The detection band＞ | Detect band ≈ | The detection band＜ | Detection is with 0 | |
| With reference to the band colour developing | Do not contain | 1＜ | ＞1 | ＞5 |
Last table is the unit of detection limit with ppb or ng/ml all.Detect band＞, ≈,＜, the 0th, and relatively with reference to the band color.
Claims (3)
1, Veritying test paper of oxytetracycline gel, comprise the nitrocellulose filter (6) that is bonded on the liner plate (7), it is characterized in that constituting composite bed along vertical surface compartment of terrain set gold mark terramycin monoclonal antibody (2), terramycin coupled antigen (3), the anti-mouse IgG antibody (4) successively from bottom to top of nitrocellulose filter (6).
2, Veritying test paper of oxytetracycline gel according to claim 1 is characterized in that connecting with last adsorptive pads (5) in nitrocellulose filter (6) upper end; Lower end or be connected with following adsorptive pads (1) by gold mark terramycin monoclonal antibody (2), or directly connect with following adsorptive pads (1).
3, Veritying test paper of oxytetracycline gel according to claim 1 is characterized in that at down adsorptive pads (1), gold mark terramycin monoclonal antibody (2) and goes up adsorptive pads (5) surface and post self-adhesive paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620019378 CN2893708Y (en) | 2006-03-23 | 2006-03-23 | Terramycin collaurum method detection test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620019378 CN2893708Y (en) | 2006-03-23 | 2006-03-23 | Terramycin collaurum method detection test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2893708Y true CN2893708Y (en) | 2007-04-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620019378 Expired - Fee Related CN2893708Y (en) | 2006-03-23 | 2006-03-23 | Terramycin collaurum method detection test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2893708Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1818658B (en) * | 2006-03-09 | 2010-05-12 | 云南大学 | Veritying test paper of oxytetracycline gel and production thereof |
| CN102043057A (en) * | 2010-11-11 | 2011-05-04 | 哈尔滨工业大学 | Test strip for testing oxytetracycline residue in meat and aquatic products and preparation method thereof |
-
2006
- 2006-03-23 CN CN 200620019378 patent/CN2893708Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1818658B (en) * | 2006-03-09 | 2010-05-12 | 云南大学 | Veritying test paper of oxytetracycline gel and production thereof |
| CN102043057A (en) * | 2010-11-11 | 2011-05-04 | 哈尔滨工业大学 | Test strip for testing oxytetracycline residue in meat and aquatic products and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070425 |