CN2859501Y - Excretion collecting device for root system of plant - Google Patents
Excretion collecting device for root system of plant Download PDFInfo
- Publication number
- CN2859501Y CN2859501Y CN 200520023038 CN200520023038U CN2859501Y CN 2859501 Y CN2859501 Y CN 2859501Y CN 200520023038 CN200520023038 CN 200520023038 CN 200520023038 U CN200520023038 U CN 200520023038U CN 2859501 Y CN2859501 Y CN 2859501Y
- Authority
- CN
- China
- Prior art keywords
- root
- gathering
- retaining
- vacuum pump
- buffer container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The utility model is about a home position root exudate gathering unit, the whole said device include: Root department growth directional guide part, circulating fluid eluation portion, vavuum pump filter, and buffer container. Above-mentioned root department growth direction guide part comprises of root shielding cylinder,soil layer space,looped type root shielding screen mesh, soil shielding teflon membrane,basal manure layer space and root shielding nylon net; Circulating fluid wash eluation portion comprises of fluid separating wall outside of circulating fluid wash eluation portion and circulating fluid pipe with valve, Circulate fluid may be aseptic distilled water, simple calcium solution, plant growth nutrient solution or organic solution; Portion of vacuum pump filter and buffer container are comprised by mini vacuum pump (0.1MPa) and buffer container ( with 3 ports ); Among which , size of root shielding nylon net mesh is smaller than 30 mum , may prevent root department from going through, and will not have an influence on free flow of water,solute and gas.
Description
Technical field
The utility model belongs to the gathering-device of root exudations.
Background technology
The solution collecting method is adopted in the collection of root secretion at present mostly, or solution and substrate culture (silica sand or other matrix) mixed collection method.For example, 1964, the root secretion extraction element of Clayton design was that eluate is root secretion after handling with the exposed aerial root system of the method drip washing of spraying.Nineteen eighty-two, the root exudates continuously trapping system of Tang and Young design.1994, Shepherd and Davies designed the natural extraction element of root exudates under the sand culture condition.2000, Jia Liming designed recirculated water root exudates gathering-device on forefathers' basis.2003, Liao Jipei etc. designed a kind of new type of continuous root secretion gathering-device (seeing Chinese patent CN2581964Y and CN1482443A for details).The gathering-device of above-mentioned root exudates all has a weak point: be not under edaphic condition, can not original position collect, influenced the root exudates dna purity.
Summary of the invention
The purpose of this utility model provides a kind of not disturbance root system normal growth, does not destroy the rhizosphere soil body, and is simple in structure, with low cost, can carry out the device of original position collection in real time, fast to root exudations under the earth culture condition.
For achieving the above object, the utility model adopts following technical scheme:
The utility model secretions from plant roots gathering-device, comprising container, the root growth directional guide part that is arranged on container central authorities, the recirculated water wash-out part that links to each other with container, be installed in recirculated water wash-out suction filtration and buffer container partly, and the vacuum pump that links to each other with buffer container with suction filtration.
Wherein said root growth directional guide partly comprises root shielding cylinder, the ring-like retaining root screen cloth of arranging successively to the periphery from the center, keeps off native poly tetrafluoroethylene, retaining root nylon wire is formed, be the soil horizon space between root shielding cylinder and the ring-like retaining root screen cloth wherein, keeping off between native poly tetrafluoroethylene and the retaining root nylon wire is the hypothallus space.
Wherein said recirculated water wash-out comprises that partly being close to retaining root nylon wire forms the water-resisting wall of outside and the circulating water pipe of band valve, and circulation fluid is sterile distilled water, simple calcium solution, plant growth nutrient fluid or organic solution.
The maximum pressure 0.1Mpa of wherein said vacuum pump.
Wherein said buffer container has three ports, and one of them port is communicated with circulating water pipe, and another port is communicated with water-resisting wall, and the 3rd port is communicated with vacuum pump.
The mesh of wherein said retaining root nylon wire is less than 30 μ m.
According to the technical solution of the utility model contrast prior art, have following advantage: when collecting root exudates the not disturbance rhizosphere soil body, do not injure the root system normal growth; The quantity of root exudates is more in the unit volume collection liquid; Material is common, making, easy to operate; The experiment favorable reproducibility; The energy real-time collecting; The actual conditions that root exudates that the background interference of soil or plant residue degradation product collects simultaneously can reflect the root system secretion have more truly been reduced to a great extent.
Description of drawings
Fig. 1 is the utility model secretions from plant roots gathering-device structural representation;
Fig. 2 is a column type framework A structural drawing;
Fig. 3 is built-in retaining root cylindrical structure figure;
Fig. 4 is annular retaining root mesh structure figure;
Fig. 5 is a column type framework B structural drawing;
Fig. 6 is the water-resisting wall structural drawing;
Fig. 7 is an organic glass container part structural drawing.
Embodiment
Root exudations original position gathering-device is by root growth directional guide part, and recirculated water wash-out part and decompression, buffer container part (as Fig. 1) are formed.Wherein, the root growth directional guide partly is that this device is crucial, is respectively that promptly the space that forms, ring-like retaining root screen cloth 3 (being connected retaining root right cylinder), the native poly tetrafluoroethylene 4 of retaining, hypothallus space 5, retaining root nylon wire 6 (less than 30 μ m) form with keeping off native poly tetrafluoroethylene to separate naturally by retaining root right cylinder for retaining root right cylinder 1 (being positioned at the device bosom), soil horizon space 2-from center to periphery.Retaining root right cylinder can be by stopping that the inert material that root system passes makes.But the soil that the soil horizon interior volume can any growing plant of splendid attire.It is to have certain degree of hardness, root system not to pass that ring-like retaining root screen cloth requires, and its active component does not exert an influence to root growth, and do not influence soil nutrient, freely the passing through of moisture, its material can be nylon grenadine, nylon taffeta, microporous barrier or woven wire etc.The thickness requirement of poly tetrafluoroethylene is less than 10 μ m, and the assurance root system can be worn out.The inert base little to the root secretion adsorbability, that composition is single (as the silica sand that cleans up) is equipped with in the hypothallus space requirement.Nylon wire requires: root secretion is passed through continuously, stop root system of plant to penetrate again and enter soil layer; Root secretion is adsorbed less, has certain toughness; Its active component crop root growth is had no adverse effect and price suitable.Recirculated water wash-out part is respectively that water-resisting wall 7, circulating water pipe 8 and inner circulation solution are formed from center to periphery.That water-resisting wall requires is waterproof, by the little inert material of root secretion absorption is formed.Circulating water pipe can be that internal diameter is silicone tube or the teflon tube of 1-10mm.Circulation solution can be sterile distilled water, 0.2-0.5mM calcium solution etc.Vacuum pump suction filtration, buffer container part are by minipump 9 (maximum pressure is 0.1Mpa), and buffer container 10 (its volume is 5 liters greater than the recirculated water volume) is formed.Wherein to have three adjustable port be respectively inlet 14 (upper end is connected with water-resisting wall), liquid outlet 12 (lower end is communicated with circulating water pipe), vacuum pump bleeding point 13 (left upper end is communicated with vacuum pump) to buffer container.
The method of the above-mentioned whole device of manufacturing process can be divided into three steps:
1), the design of root growth directional guiding device: making column type framework A as shown in Figure 2 (highly: 30cm earlier; Internal diameter: d=20cm), material can be stainless steel material, is used to support poly tetrafluoroethylene.Built-in retaining root right cylinder 1 is to be made by cement and sand composite material, as shown in Figure 3 (highly: 20cm; Internal diameter: d=10cm), and highly locate to inlay annular retaining root screen cloth 3 (material: stainless steel, as Fig. 4) at retaining root right cylinder side 15cm, the upper end of annular retaining root screen cloth links with retaining root right cylinder, lower end and the binding of poly tetrafluoroethylene 4 inside surfaces.Be used to support the column type framework B of nylon wire, as shown in Figure 5 (highly: 20cm; Internal diameter: d=25cm), material also is a stainless steel material, and the right cylinder bottom surface is airtight.
2) recirculated water wash-out part: make splendid attire circulation fluid organic glass container part earlier (highly: 30cm; Internal diameter: d=30cm is as Fig. 7), the right cylinder bottom surface is airtight, and stays an aperture (internal diameter is d=1cm) at right cylinder one side height 25cm place.Water-resisting wall is for having certain plastic rubber material, and it highly is 22cm (as Fig. 6).Circulating water pipe can be that internal diameter is silicone tube or the hard teflon tube of 1-10mm.
3) vacuum pump suction filtration, buffer container part: by small-sized circulation ability of swimming vacuum pump (specification a: SHZ-D (III), 100 watts of power), buffer container is that rectangular build (specification: make by 20 * 15 * 15cm) pmma material, two controlled ports are arranged at its top, being positioned at RC (14) liquid outlet is connected with water-resisting wall, guarantee that aqueous solution can circulate, another opening links to each other with vacuum pump; One controlled port (as shown in Figure 1) is arranged at its bottom.(11) T-valve of its downside is used for root secretion and flows out and circulation fluid recycle.
The device operating process is as follows:
1) root growth directional guide: comprise for the pre-service that tries soil, sow, grow seedlings and daily management.To sterilize, be divided into two parts earlier for examination soil, A part does not add any fertilizer, and B part adds NPK compound fertilizer (improve the available nutrient of soil more than at least 50%, help the root system directional guide).By dividing plate, A part soil is placed directly over the retaining root right cylinder; B part soil is added the cylindrical side of retaining root respectively.Meanwhile, outside soil layer, add the matrix after cleaning, sterilizing respectively, and guarantee the integrality of poly tetrafluoroethylene.After soil and matrix have been loaded, sow in soil, every basin plantation seed 10-20 grain is because of the individual size of crop is adjusted.After waiting to emerge, keep Suitable Density, treat that plant strain growth is used for the collection of root secretion after a period of time.Experimental result shows that this directional guide measure can make Root Distribution more than 60% in hypothallus, guarantees to collect the root secretion of q.s.
2) cleaning of root exudates collection system: before root secretion is collected, add sterile distilled water and follow the ring water pipe and enter, and be full of space beyond the water-resisting wall, cover the water-resisting wall top then, enter into hypothallus, make the moisture state that reaches capacity.Stop supplies moisture left standstill 30 minutes then.Restart vacuum pump, suction filtration 5-10 minute, the aqueous solution of hypothallus entered buffer container and discharges, this process that circulates about 3 times, can remove the degradation product of soil, matrix or root system organic substance, make whole collection system " cleaning ", be convenient to the collection of root exudates.
3) collection of root exudates: after whole root exudates collection system is cleaned and finished,, keep plant root after secretion a period of time, add sterile distilled water again and make the hypothallus water saturation, left standstill 30 minutes according to the different experiments processing requirements.Start vacuum pump, suction filtration 5-10 minute, the aqueous solution of hypothallus entered buffer container, take out then, and refrigeration (4 ℃), to be measured.With the said process triplicate, obtained aqueous solution is root secretion and collects liquid.
Enforcement should also be noted that following item according to the technical solution of the utility model:
1) in the dress basin process, prevents the poly tetrafluoroethylene breakage.
2) density of crop is according to individuality is big or small, particularly the size of root biomass is decided.
3) the root secretion acquisition time is decided because of the Different Crop characteristic.
4) the reduced vacuum suction filtration can not surpass 0.1Mpa, otherwise influential to root growth.
5) thickness of poly tetrafluoroethylene should be moderate, is generally 5-10 μ m.
6) this device also can be used for collecting water-insoluble root exudates, and promptly circulation fluid can be changed to other organic solvent.
Claims (6)
1, a kind of secretions from plant roots gathering-device, it is characterized in that comprising container, be arranged on container central authorities root growth directional guide part, the recirculated water wash-out part that links to each other with container, be installed in recirculated water wash-out suction filtration and buffer container (10) partly, and the vacuum pump (9) that links to each other with buffer container with suction filtration.
2, secretions from plant roots gathering-device according to claim 1, it is characterized in that described root growth directional guide partly comprises retaining root right cylinder (1), the ring-like retaining root screen cloth (3) of arranging successively to the periphery from the center, keeps off native poly tetrafluoroethylene (4), retaining root nylon wire is formed (6), wherein keeping off between root right cylinder (1) and the ring-like retaining root screen cloth (3) is soil horizon space (2), keeps off between native poly tetrafluoroethylene (4) and the retaining root nylon wire (6) being hypothallus space (5).
3, secretions from plant roots gathering-device according to claim 2, it is characterized in that described recirculated water wash-out comprises that partly being close to retaining root nylon wire forms the water-resisting wall (7) of (6) outside and the circulating water pipe (8) of band valve, circulation fluid is sterile distilled water, simple calcium solution, plant growth nutrient fluid or organic solution.
4, secretions from plant roots gathering-device according to claim 3 is characterized in that the maximum pressure 0.1Mpa of described vacuum pump (9).
5, secretions from plant roots gathering-device according to claim 4, it is characterized in that described buffer container (10) has three ports, one of them port is communicated with circulating water pipe (8), and another port is communicated with water-resisting wall (7), and the 3rd port is communicated with vacuum pump (9).
6, according to the arbitrary described root system gathering-device of claim 1-5, the mesh that it is characterized in that described retaining root nylon wire is less than 30 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520023038 CN2859501Y (en) | 2005-06-20 | 2005-06-20 | Excretion collecting device for root system of plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520023038 CN2859501Y (en) | 2005-06-20 | 2005-06-20 | Excretion collecting device for root system of plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2859501Y true CN2859501Y (en) | 2007-01-17 |
Family
ID=37612360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520023038 Expired - Fee Related CN2859501Y (en) | 2005-06-20 | 2005-06-20 | Excretion collecting device for root system of plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2859501Y (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766100B (en) * | 2009-12-25 | 2011-07-20 | 山东省农业科学院土壤肥料研究所 | Device for researching plant root exudate allelopathy and application method thereof |
| CN102175487A (en) * | 2011-01-11 | 2011-09-07 | 南京大学 | In-situ collection device for plant root secretions and application thereof |
| CN101598638B (en) * | 2009-06-19 | 2011-09-14 | 沈阳大学 | Device for collecting root exudate in situ |
| CN103293035A (en) * | 2012-03-05 | 2013-09-11 | 华中农业大学 | Root box test device suitable for growing of plants |
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN104006988A (en) * | 2014-05-26 | 2014-08-27 | 上海大学 | Conical surface percolation type device and method for collecting root exudates of soil cultivation plants |
| CN104115689A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院东北地理与农业生态研究所 | Live crop root compartment cultivation device and using method |
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN106982628A (en) * | 2017-04-01 | 2017-07-28 | 内蒙古农业大学 | A kind of interior growth core system and method for determining desert steppe root biomass |
| CN107036873A (en) * | 2017-05-09 | 2017-08-11 | 黑龙江大学 | A kind of device and method of in-situ extraction arbuscular mycorrhiza secretions from plant roots |
| CN107646411A (en) * | 2017-10-17 | 2018-02-02 | 沈阳大学 | A kind of plant root growth experimental provision |
| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
-
2005
- 2005-06-20 CN CN 200520023038 patent/CN2859501Y/en not_active Expired - Fee Related
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101598638B (en) * | 2009-06-19 | 2011-09-14 | 沈阳大学 | Device for collecting root exudate in situ |
| CN101766100B (en) * | 2009-12-25 | 2011-07-20 | 山东省农业科学院土壤肥料研究所 | Device for researching plant root exudate allelopathy and application method thereof |
| CN102175487A (en) * | 2011-01-11 | 2011-09-07 | 南京大学 | In-situ collection device for plant root secretions and application thereof |
| CN102175487B (en) * | 2011-01-11 | 2013-01-02 | 南京大学 | In-situ collection device for plant root secretions and application thereof |
| CN103293035A (en) * | 2012-03-05 | 2013-09-11 | 华中农业大学 | Root box test device suitable for growing of plants |
| CN103293035B (en) * | 2012-03-05 | 2016-03-02 | 华中农业大学 | A kind of root case test unit being applicable to planting plants |
| CN103776662B (en) * | 2014-01-22 | 2016-01-27 | 福建农林大学 | A kind of xerophyte root exudates gathering-device and collection method thereof |
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN104006988A (en) * | 2014-05-26 | 2014-08-27 | 上海大学 | Conical surface percolation type device and method for collecting root exudates of soil cultivation plants |
| CN104115689B (en) * | 2014-07-04 | 2015-12-09 | 中国科学院东北地理与农业生态研究所 | A kind of live body crop root locellus culture apparatus and using method |
| CN104115689A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院东北地理与农业生态研究所 | Live crop root compartment cultivation device and using method |
| CN105115780B (en) * | 2015-08-24 | 2018-09-28 | 成都中医药大学 | Plant rhizosphere soil and root rhizosphere secreta collection device and collection method |
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN105973632B (en) * | 2016-05-05 | 2019-01-25 | 中国农业科学院烟草研究所 | Secretions from plant roots generation and collection device and its collection method |
| CN106982628A (en) * | 2017-04-01 | 2017-07-28 | 内蒙古农业大学 | A kind of interior growth core system and method for determining desert steppe root biomass |
| CN107036873A (en) * | 2017-05-09 | 2017-08-11 | 黑龙江大学 | A kind of device and method of in-situ extraction arbuscular mycorrhiza secretions from plant roots |
| CN107646411A (en) * | 2017-10-17 | 2018-02-02 | 沈阳大学 | A kind of plant root growth experimental provision |
| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN108519250B (en) * | 2018-04-18 | 2024-02-27 | 中国科学院水生生物研究所 | Device for collecting submerged plant root secretions in situ and use method |
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN109406244B (en) * | 2018-12-29 | 2023-10-10 | 中国科学院南京地理与湖泊研究所 | Aquatic plant root system secretion normal position collection device |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2859501Y (en) | Excretion collecting device for root system of plant | |
| CN105684868B (en) | A kind of hydroponic plant wall method for planting and device | |
| CN100387961C (en) | Rock growing plant root secretion and circulation liquid collecting device and its collecting method | |
| CN106336253A (en) | Production process for producing organic fertilizer through concentrating biogas slurry by using multistage membrane separation technology | |
| CN102636373A (en) | Multi-channel plant root exudate collection system and collection method thereof | |
| CN105115780A (en) | Plant rhizosphere soil and rhizosphere exudate collecting device and method | |
| CN204085951U (en) | A kind of sandy culture collects the device of secretions from plant roots | |
| CN203563477U (en) | Closed type pipeline substrate culturing device | |
| CN106769283A (en) | Experiment Root of Wetland Plants secretion continuously trapping system and continuous collection method | |
| CN100359312C (en) | Plant root excretion continuous collection method and apparatus | |
| CN113261449A (en) | Planting and cultivating device for Chinese redbud saplings and implementation method of planting and cultivating device | |
| CN203388036U (en) | Large-scale water culture soilless culture device | |
| CN209017671U (en) | A kind of prawn ecological cultivation device | |
| CN207802768U (en) | A kind of liquid manure trickle irrigation retracting device | |
| JP2013170952A (en) | Apparatus and method for removing contaminant by using cocoon | |
| CN106134686A (en) | The method that Herba Cistanches is efficiently parasitic | |
| KR200486448Y1 (en) | An apparatus for separating nutrient from hydroponic waste solution | |
| CN211407313U (en) | Australia freshwater lobster breeding water treatment system | |
| CN211532240U (en) | Water planting vegetable seedling conveyer | |
| CN204634758U (en) | A kind of fresh water seed semi-aerobic landfill system | |
| CN100522839C (en) | Silver carp, bighead carp, grass carp, cladophora and daphnia hyaline cooperated algae control method | |
| CN209513361U (en) | A kind of water planting collection device of secretions from plant roots | |
| CN211909807U (en) | Get convenient gardens of material and use device of growing seedlings | |
| KR101428553B1 (en) | Method for purifying veterinary antibiotics in water using biochar derived from burcucumber(Sicyos angulatus L.) | |
| CN209376479U (en) | A kind of fish and vegetable symbiotic cultivation equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070117 Termination date: 20100620 |