CN2841667Y - The cell nutsche filter - Google Patents
The cell nutsche filter Download PDFInfo
- Publication number
- CN2841667Y CN2841667Y CNU2005200215974U CN200520021597U CN2841667Y CN 2841667 Y CN2841667 Y CN 2841667Y CN U2005200215974 U CNU2005200215974 U CN U2005200215974U CN 200520021597 U CN200520021597 U CN 200520021597U CN 2841667 Y CN2841667 Y CN 2841667Y
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- CN
- China
- Prior art keywords
- cell
- nutsche filter
- cylinder
- commissioned
- train
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The cell nutsche filter.In the present former foster process of being commissioned to train of cell, basic skills with proteopepsis enzymolysis dispersion tissue when cell is the vitro culture zooblast, but after digestion and repeatedly in the digestive process, it is thorough inadequately to draw supernatant, it is not easily separated to have left over part cell and tissue block, cause the waste of part cell or cell dissociation overlong time and vigor descends, so that formerly be commissioned to train that success ratio reduces when supporting.The cell nutsche filter, its composition comprises: cylinder 1, the tube end of described cylinder 1, have metal screen 2.This product is used for chorista piece and cell or cell mass.
Description
Technical field: the utility model relates to a kind of cell nutsche filter.
Background technology: in the present former foster process of being commissioned to train of cell, basic skills with proteopepsis enzymolysis dispersion tissue when cell is the vitro culture zooblast, but after digestion and repeatedly in the digestive process, it is thorough inadequately to draw supernatant, it is not easily separated to have left over part cell and tissue block, cause the waste of part cell or cell dissociation overlong time and vigor descends, so that formerly be commissioned to train that success ratio reduces when supporting.
Summary of the invention: the purpose of this utility model provides a kind of cell nutsche filter that is used for chorista piece and cell or cell mass.
Above-mentioned purpose realizes by following technical scheme:
This technical scheme has following beneficial effect:
The cell nutsche filter, its composition comprises: cylinder, the tube end of described cylinder, have metal screen.
Above-mentioned cell nutsche filter, described cylinder adopts the polymer transparent tube.
Above-mentioned cell nutsche filter, described cylinder are contained in centrifuge tube or the culture tube, and described centrifuge tube is a standard 50ml centrifuge tube.
Beneficial effect compared with prior art:
1. the utility model can make cell and tissue block after digestion or repeatedly in the digestive process, abundant chorista piece and the cell that digests, making the cell quantity that digests improve and can in time end digests, obviously improve cell viability, strengthen the cell attachment rate, make former being commissioned to train form power and be improved, and can make the cell mass mechanical separation become individual cells; The carrying out that helps other experiment is as cell electron microscopic examination, flow cytometer inspection etc.
2. the utlity model has acidproof, high voltage bearing effect, can be through high pressure and chemical process sterilization.
Description of drawings: accompanying drawing 1 is the structural representation of this product.
Accompanying drawing 2 is structural representations of centrifuge tube.
Accompanying drawing 3 is this product structure synoptic diagram of culture dish special use.
Accompanying drawing 4 is structural representations of culture dish.
Embodiment:
Embodiment 1:
The cell nutsche filter, its composition comprises: cylinder 1, the tube end of described cylinder 1, have metal screen 2.
Embodiment 2:
Above-mentioned cell nutsche filter, described cylinder 1 adopts the polymer transparence.
Embodiment 3:
Above-mentioned cell nutsche filter, described cylinder 1 are contained in centrifuge tube 3 or the culture tube 4, and described centrifuge tube 3 is a standard 50ml centrifuge tube.
Cylinder of the present utility model has two kinds, and a kind of is the special use of 50ml centrifuge tube, is applicable to the digestion of the tissue block that a small amount of tissue block and fibrous tissue are few, the isolated cell agglomerate; Another kind is the culture dish special use, is applicable to the digestion of the tissue block that a large amount of tissue block and fibrous tissue are many, the isolated cell agglomerate.The drum diameter of 50ml centrifuge tube special use is 1.6cm, the high 10.5cm of being; The drum diameter of culture dish special use is 3cm, the high 1cm of being.
Working process is described:
Above-mentioned cell nutsche filter, 50ml centrifuge tube special use: earlier cylinder is put into centrifuge tube, then tissue block or cell mass are put in the cylinder 1, digestion and suction pipe piping and druming by enzyme, can cell be separated fully with tissue block by repeating digestion, the cell suspension that contains pancreatin after the filtration should in time be ended digestion, and the cell after the piping and druming can make cell mass be separated into individual cells by filter membrane.
Above-mentioned cell nutsche filter, culture dish special use: earlier cylinder is put into culture dish, then tissue block or cell mass are put in the cylinder 1, digestion and suction pipe piping and druming by enzyme, can cell be separated fully with tissue block by repeating digestion, the cell suspension that contains pancreatin after the filtration should in time be ended digestion.
Claims (3)
1. cell nutsche filter, its composition comprises: cylinder is characterized in that: the tube end of described cylinder, have metal screen.
2. cell nutsche filter according to claim 1 is characterized in that: described cylinder adopts the polymer transparent tube.
3. cell nutsche filter according to claim 1 is characterized in that: described cylinder is contained in centrifuge tube or the culture tube, and described centrifuge tube is a standard 50ml centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2005200215974U CN2841667Y (en) | 2005-09-09 | 2005-09-09 | The cell nutsche filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2005200215974U CN2841667Y (en) | 2005-09-09 | 2005-09-09 | The cell nutsche filter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2841667Y true CN2841667Y (en) | 2006-11-29 |
Family
ID=37445557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2005200215974U Expired - Fee Related CN2841667Y (en) | 2005-09-09 | 2005-09-09 | The cell nutsche filter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2841667Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102893A (en) * | 2017-12-29 | 2018-06-01 | 天津普瑞赛尔生物科技有限公司 | A kind of processing equipment of stem cell extraction |
| CN108251305A (en) * | 2018-04-13 | 2018-07-06 | 常州市武进人民医院 | Primitive cell culture tissue tabernacle cage |
| CN108823062A (en) * | 2015-08-31 | 2018-11-16 | 爱平世股份有限公司 | Cell mass divider |
-
2005
- 2005-09-09 CN CNU2005200215974U patent/CN2841667Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823062A (en) * | 2015-08-31 | 2018-11-16 | 爱平世股份有限公司 | Cell mass divider |
| CN108823062B (en) * | 2015-08-31 | 2019-12-27 | 爱平世股份有限公司 | Cell mass divider |
| CN108102893A (en) * | 2017-12-29 | 2018-06-01 | 天津普瑞赛尔生物科技有限公司 | A kind of processing equipment of stem cell extraction |
| CN108251305A (en) * | 2018-04-13 | 2018-07-06 | 常州市武进人民医院 | Primitive cell culture tissue tabernacle cage |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |