CN101979521A - Complex enzyme preparation special for antibiotic dregs - Google Patents
Complex enzyme preparation special for antibiotic dregs Download PDFInfo
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- CN101979521A CN101979521A CN2010102913266A CN201010291326A CN101979521A CN 101979521 A CN101979521 A CN 101979521A CN 2010102913266 A CN2010102913266 A CN 2010102913266A CN 201010291326 A CN201010291326 A CN 201010291326A CN 101979521 A CN101979521 A CN 101979521A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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Abstract
The invention relates to a complex enzyme preparation special for antibiotic dregs, which is characterized by consisting of the following components in percentage by weight: 15 to 20 percent of xylanase, 25 to 45 percent of pectinase, 10 to 15 percent of protease, 10 to 15 percent of amylase and 20 to 25 percent of purified water. The complex enzyme preparation special for the antibiotic dregs can effectively reduce the water content of the antibiotic dregs, reduce the viscosity of materials, and can effectively degrade proteins, starch polysaccharides, non-starch polysaccharides and the like in the antibiotic dregs to reduce the viscosity; nutrient substances in the antibiotic dregs after degradation can be easily used for fermenting methane; and the problems of surface pollution and air pollution caused by cassava waste residues are greatly solved, and the double effects of recycling the waste residues and saving energy and reducing emission are achieved.
Description
Technical field
The present invention relates to microbiotic dregs of a decoction complex enzyme preparation special and application method
Technical background
The microbiotic dregs of a decoction are the filter residue of microbiotic bulk drug fermented product, and its main component is: biological mediums such as corn steep liquor, peptone, starch, soya-bean oil, soybean cake powder.Moisture content reaches 90% in the antibiotic dregs of a decoction owing to having separated, and the solid dregs of a decoction are evenly dispersed in the liquid with the form of suspension, mostly adopt fermentation vat to stack or buried way processing all the time, not only wasted the effectively utilization of nutritive substance in the microbiotic dregs of a decoction, and because the effect of the microorganism species of medical chemistry preparation in the depleted microbiotic dregs of a decoction and complexity has stayed secular toxic hidden danger to the mankind.Because national environmental protection department efforts of investigation and prosecution strengthens at present, each production of antibiotics enterprise generally adopts the quadruple effect evaporation, and energy consumption is very big, has brought very big pressure to enterprise, and this method does not meet energy-saving and cost-reducing that country proposes, the target of low-carbon economy yet.Show according to web material: there are 81 families in the enterprise of the microbiotic of China's production at present bulk drug, produce about 1.4 hundred million tons of the microbiotic dregs of a decoction per year, annual each microbiotic production of raw medicine enterprise all will spend the environment protection treating (needing land acquisition to deposit and pay to relevant department effluent charge, the cleaning expense of certain number) etc. that great amount of manpower, financial resources are used for the microbiotic dregs of a decoction.
Because producing microbiotic in the past generally adopts be added with medical emetic Zinc Sulphate Heptahydrate in follow-up filtration procedure, this several years rising trends of the price of Zinc Sulphate Heptahydrate are very fast, zinciferous dregs of a decoction heavy metal content exceeds standard, the dregs of a decoction of oven dry can not be used for feed and chemical fertilizer, so present production of antibiotics enterprise does not generally add the Zinc Sulphate Heptahydrate example in process of production.As: CN1093681A (producing the method and apparatus of zinc sulfate with the erythromycin waste residue) patented method that on October 19th, 1994 was announced by State Intellectual Property Office, this method is to need earlier the cold of dregs of a decoction nature to be shone to leather hard, again by a kind of special roasting kiln with coal or other fuel to the dregs of a decoction heat, heating flame, therefrom reclaim zinc oxide and produce the way of zinc sulfate with sulfuric acid reaction.Though this invention has solved the resource utilization of the dregs of a decoction and used, because the energy consumption that produces is big in process of production, cost is high, run into can't produce again in rainy season or winter, and residual sour waste residue, waste liquid after filtering have polluted environment again.Because no obvious social and economic benefit do not obtain promotion and application for many years always.And for example on May 11st, 2005, State Intellectual Property Office announced: by CN200310108527 (treatment process that the contains zinc antibiotic bacterium slag) patent of Zhejiang Polytechnical University's invention, the method of this patent be utilize hydrochloric acid, sulfuric acid or acetic acid and water with the dregs of a decoction dilution of alkalescence, transfer to acidity, then with the metallic zinc in the chelating ion exchange resin absorption dregs of a decoction, with heavy dose of acid or hydrochlorate the zinc in the resin is broken away from again, finish separating of zinc and the dregs of a decoction, use with the resource utilization that realizes the dregs of a decoction.But because this method has been utilized a large amount of acid supplements and water resources, the pollution of discharging is not sayed and is spoken.The more important thing is that heavy dose of acid supplement and expensive ion exchange resin (owing to the restriction of loading capacity and regenerant consumption, being mainly used in the recovery of rare noble metal) are used for waste dregs generated application, production cost is well imagined.So the resource utilization of the microbiotic dregs of a decoction, innoxious application are still problem anxious to be solved at present.And for example relate to a kind of method of utilizing erythromycin waste residue to reclaim zinc and produce Yeast protein with the erythromycin waste residue after the dezincify in the patent of invention 200610005585, this method of this method is to utilize reactor to measure the content of zinc in the wet erythromycin waste residue, again extraction agent is put in the aforesaid reaction vessel, in reactor, material is carried out solid-liquid separation, solution after the separation is hydrolyzed or electrolysis, directly get zinc powder after the electrolysis, the dregs of a decoction after will separating at last are used for protein fodder maybe with after this dregs of a decoction sterilization, the inoculation yeast bacterial classification ferments, and produces Yeast protein.Because this method adopts reactor that zinc is separated, after the zinc extraction, erythromycin waste residue still is in the suspension state, also needs the quadruple effect evaporation liquid evaporation could be gone out.Fundamentally do not solve the high problem of erythromycin waste residue moisture content.
The difficult analysis of causes of microbiotic dregs of a decoction dehydration: the moisture 85%-90% of the microbiotic dregs of a decoction, dry-matter 10%-15% is a kind of flowability suspension preferably.Solid matter is evenly dispersed in the solution.Macromolecular substance content height such as protein and xylan in this suspension, be connected with covalent linkage, hydrogen bond, ehter bond and ester bond respectively between these polymers, they and formations such as albumen, xylan and pectin have certain degree of hardness and elastic cell walls, thereby present stable chemical structure.By above reason as can be known: 1. because materials such as albumen, starch granules, xylan overlapped blocked the necessary physical channel of draining; The material uniform distribution of 2. various formation plant cell walls had not only blocked the draining space but also because of itself having certain toughness, and effect such as surface tension, increased resistance of water drainage; 3. the capillary action between each solid matter, particularly fiber and interfibrous capillary action also increase the resistance of draining; 4. most of colloidalmaterials such as pectin substance are hydrophilic micelle, and positive charge is attracting each other in fiber and half fiber surface negative charge and the water, and these molecules or interionic reactive force strengthen the solid-liquid separation difficulty; 5. because the effect that albumen has very strong absorption and increases viscosity, so volume change often takes place being subjected to external force to do the time spent, cause small draining duct to become more tiny or stop up, be to increase one of difficulty that the microbiotic dregs of a decoction fully dewater.
Summary of the invention
The objective of the invention is from reducing the water content of the microbiotic dregs of a decoction in essence, reach solid-liquid separation, the dried slag of solid is produced perfect compound feed, liquid is owing to contain rich nutrient substances, the fermentative production that can be used for biogas, the present invention is for providing a kind of microbiotic dregs of a decoction complex enzyme preparation special and application method.
The concrete prescription of the present invention is as follows, the composition that content is weight percentage:
Zytase 15-20%, polygalacturonase 25-35%, proteolytic enzyme 10-25%, amylase 10-15%.Pure water 20-25%
Preparation method of the present invention is: with dhdps enzyme respectively through pre-treatment, micro-filtrate membrane filtration, it is composite to enter automatic mixing machine more in proportion, by smart membrane filtration, quantitative filling, becomes product then.
Microbiotic dregs of a decoction complex enzyme preparation special of the present invention, above-mentioned zytase is made of following component in percentage by weight:
Zytase 80%, beta-glucanase 20%.
Microbiotic dregs of a decoction complex enzyme preparation special of the present invention, above-mentioned polygalacturonase is made of following component in percentage by weight:
Pectin lyase 45%, Rohapect MPE 55%.
Microbiotic dregs of a decoction complex enzyme preparation special of the present invention, above-mentioned proteolytic enzyme is made of following component in percentage by weight:
Neutral protease 25%, papoid 75%.
Microbiotic dregs of a decoction complex enzyme preparation special of the present invention, above-mentioned amylase are middle temperature α-Dian Fenmei.
Physics and chemistry and biological property at the microbiotic dregs of a decoction, the process characteristic of combination antibiotic manufacturing enterprise, utilize the water content of method degraded macromole viscous substance (as pectin, albumen, xylan) the reduction microbiotic dregs of a decoction of compound bio-enzyme, from carrying out solid-liquid separation in essence, solid is used for complete feed, and liquid is used for the production of biogas.Compound bio-enzyme action condition gentleness has fundamentally been removed the high problem of dregs of a decoction water content, avoids a large amount of dregs of a decoction buried, or enters rivers, farmland, and waste residue is turned waste into wealth, and also is important measures of comprehensive regulation environmental pollution.
The present invention has following distinguishing feature from the principle of various enzymes:
1, enough zytase: the xylan in can hydrolysis material, can destroy the skein cell wall construction that its chain-like structure and hemicellulose thereof constitute, solved the xylan water-swelling and weave in, reduce the water content of material.
2, enough polygalacturonase: by polygalacturonase macromole pectin is degraded, generate low-molecular-weight galacturonic acid, solved the physical channel and stopped up in the material that causes and comprise large quantity of moisture.
3, an amount of proteolytic enzyme: vegetable-protein itself has certain viscosity in water, reduces material viscosity by protolysate, reduces surface tension, reduces resistance of water drainage.
4, an amount of amylase: collaborative zytase and polygalacturonase acting in conjunction can be degraded to monose with the macromolecules starch particle in residual starch, thereby reduce the viscosity height that causes because of starch granules.
Embodiment:
1, the technological process of production of the present invention: the composition that qualified after testing raw material is weight percentage by following content enters every dog-house: zytase 20%, polygalacturonase 25%, proteolytic enzyme 15%, amylase 15%, pure water 25%.The preparation method is: through micro-filtrate membrane filtration (the microfiltration membrane aperture is 0.8 μ m), it is composite to enter automatic mixing machine more in proportion, by smart membrane filtration (smart filter membrane aperture is 0.45 μ m), quantitative filling, becomes product then with dhdps enzyme.
2, using method of the present invention: the bright microbiotic dregs of a decoction add prozyme and stir, ratio in 5/1000ths to ten thousand/(V/V) is added, at 30-60 ℃, enzymolysis 5-25h under the condition of nature pH value, after the hydrolysis liquid feed liquid is extracted out, the fermentative production that is used for biogas, solid slag becomes perfect compound feed 45 ℃ of oven dry.
3, the processing condition of auxiliary agent of the present invention:
Operative temperature: 30-60 ℃ of (or natural temperature) pH value: 4.0-7.0 (or natural pH)
Action time: 5-25h addition: 100mL-5000mL/ ton material
4, to use the lab scale situation as follows in the present invention, and ordinary method is organized in contrast and compared experiment, result such as following table:
The laboratory simulation Production Flow Chart is carried out, and gets the bright microbiotic dregs of a decoction (4kg), adds complex enzyme preparation special, under natural temperature, and solid-liquid separation behind the 5-25h, the solid oven dry, to be checked, do blank contrast under the similarity condition, experimental data is as follows:
5, experiment conclusion:
1) using the present invention can turn waste into wealth the microbiotic dregs of a decoction, prevents that the dregs of a decoction from entering the pollution of river to the face of land and air, reaches the energy-saving and emission-reduction of country's promotion, the standard of low-carbon economy.
2) use the present invention and can reduce the wastewater discharge of microbiotic dregs of a decoction manufacturing enterprise 90%.
3) use the cycle that the present invention can reduce the business processes microbiotic dregs of a decoction greatly, improve the comprehensive utilization of enterprise waste residue.
4) use the fermentative production that the present invention can be directly used in isolated liquid biogas.
5) use the present invention and the dried dregs of a decoction can be used for perfect compound feed.
Concrete facility example
Embodiment 1: the present invention specifically fills a prescription as follows, the composition that content is weight percentage: zytase 20%, polygalacturonase 25%, proteolytic enzyme 15%, amylase 15%, pure water 25%.
The preparation method is: with dhdps enzyme respectively through pre-treatment, micro-filtrate membrane filtration, it is composite to enter automatic mixing machine more in proportion, by smart membrane filtration, quantitative filling, becomes product then.
Embodiment 2: the present invention specifically fills a prescription as follows, the composition that content is weight percentage: zytase 15%, polygalacturonase 35%, proteolytic enzyme 10%, amylase 15%, pure water 25%.The preparation method is identical with example 1.
Embodiment 3: the present invention specifically fills a prescription as follows, the composition that content is weight percentage: zytase 15%, polygalacturonase 30%, proteolytic enzyme 25%, amylase 10%, pure water 20%.The preparation method is identical with example 1.
Claims (5)
1. microbiotic dregs of a decoction complex enzyme preparation special is characterized in that it is the prozyme that the one-tenth by following weight per-cent is grouped into:
Zytase 15-20%
Polygalacturonase 25-35%,
Proteolytic enzyme 10-25%,
Amylase 10-15%.
Pure water 20-25%
2. microbiotic dregs of a decoction complex enzyme preparation special as claimed in claim 1 is characterized in that above-mentioned zytase is made of following component in percentage by weight:
Zytase 80%,
Beta-glucanase 20%.
3. microbiotic dregs of a decoction complex enzyme preparation special as claimed in claim 1 is characterized in that above-mentioned polygalacturonase is made of following component in percentage by weight:
Pectin lyase 45%,
Rohapect MPE 55%.
4. microbiotic dregs of a decoction complex enzyme preparation special as claimed in claim 1 is characterized in that above-mentioned proteolytic enzyme is made of following component in percentage by weight:
Neutral protease 25%
Papoid 75%
5. microbiotic dregs of a decoction complex enzyme preparation special as claimed in claim 1 is characterized in that above-mentioned amylase is middle temperature α-Dian Fenmei.
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| CN2010102913266A CN101979521A (en) | 2010-09-26 | 2010-09-26 | Complex enzyme preparation special for antibiotic dregs |
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| CN2010102913266A CN101979521A (en) | 2010-09-26 | 2010-09-26 | Complex enzyme preparation special for antibiotic dregs |
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| CN101979521A true CN101979521A (en) | 2011-02-23 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865815A (en) * | 2014-02-25 | 2014-06-18 | 北京观澜科技有限公司 | Method for preparing biological humic acid bacteria agent from biological organic enzymolysis-coupled conversion antibiotic dregs |
| CN104328141A (en) * | 2014-10-30 | 2015-02-04 | 宁夏乙征生物工程有限公司 | Method for treating antibiotic residues by enzymic method |
| CN106267676A (en) * | 2016-09-26 | 2017-01-04 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs xylanase enzyme solution |
| CN106362346A (en) * | 2016-09-26 | 2017-02-01 | 齐鲁工业大学 | Method for enzymolysis of antibiotic waste mycelium through mannase |
| CN106465789A (en) * | 2016-09-26 | 2017-03-01 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs pectinase enzymatic hydrolysis method |
| CN106492397A (en) * | 2016-09-26 | 2017-03-15 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs saccharifying enzyme enzyme solution |
| CN106492396A (en) * | 2016-09-26 | 2017-03-15 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs laccase enzyme solution |
| CN113749178A (en) * | 2020-06-02 | 2021-12-07 | 杭州俊丰生物工程有限公司 | Enzyme treatment method of beta-lactam antibiotic and intermediate bacterial dregs thereof |
-
2010
- 2010-09-26 CN CN2010102913266A patent/CN101979521A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865815A (en) * | 2014-02-25 | 2014-06-18 | 北京观澜科技有限公司 | Method for preparing biological humic acid bacteria agent from biological organic enzymolysis-coupled conversion antibiotic dregs |
| CN103865815B (en) * | 2014-02-25 | 2015-04-22 | 北京观澜科技有限公司 | Method for preparing biological humic acid bacteria agent from biological organic enzymolysis-coupled conversion antibiotic dregs |
| CN104328141A (en) * | 2014-10-30 | 2015-02-04 | 宁夏乙征生物工程有限公司 | Method for treating antibiotic residues by enzymic method |
| CN104328141B (en) * | 2014-10-30 | 2017-10-31 | 宁夏乙征生物工程有限公司 | A kind of method for being enzymatically treated the antibiotic dregs of a decoction |
| CN106267676A (en) * | 2016-09-26 | 2017-01-04 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs xylanase enzyme solution |
| CN106362346A (en) * | 2016-09-26 | 2017-02-01 | 齐鲁工业大学 | Method for enzymolysis of antibiotic waste mycelium through mannase |
| CN106465789A (en) * | 2016-09-26 | 2017-03-01 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs pectinase enzymatic hydrolysis method |
| CN106492397A (en) * | 2016-09-26 | 2017-03-15 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs saccharifying enzyme enzyme solution |
| CN106492396A (en) * | 2016-09-26 | 2017-03-15 | 齐鲁工业大学 | A kind of antibiotic bacterium dregs laccase enzyme solution |
| CN113749178A (en) * | 2020-06-02 | 2021-12-07 | 杭州俊丰生物工程有限公司 | Enzyme treatment method of beta-lactam antibiotic and intermediate bacterial dregs thereof |
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Application publication date: 20110223 |