The abo blood group test card
Technical field:
The utility model belongs to biomedicine field, is a kind of blood type test card that detects abo blood group.
Background technology:
The technology that detects at present A, B, O blood group has multiple, comprises red blood cell salt solution coagulation, neutralization test, absorption test, separates separating test, mixed agglutination reaction, collaborative congealing reaction, radiommunoassay, enzyme linked immunosorbent assay (ELISA) etc.Wherein red blood cell salt solution coagulation is most widely used general.Red blood cell salt solution coagulation is by the blood group antigens on known IgM type antibody test fresh red blood cell surface and makes red blood cell condense judged result.Red blood cell salt solution coagulation has been widely used in the clinical blood transfusion check, is the most conventional blood group authenticate technology, mainly comprises slide method, test tube method etc.In order to use more convenient or to be used for specific area, have improving one's methods of a lot of patents.Kit has increased to the equal positive detection antibody of all abo blood groups in contrast, has increased the parts of being convenient to saving result and preventing the former body pollution of blood, and preserves the blood group card with aluminium pool bag and drying agent.Disclosed blood type test card of the utility model and kit have made things convenient for the layman to detect blood group, can effectively control the effect phase of kit, are convenient to carry out easily result's preservation and can prevent the bloodborne pathogens pollution, are convenient to preserve and transportation.
Summary of the invention:
The purpose of this utility model is a kind of blood type test card of preparation.
Be the scheme that realizes that the purpose of this utility model is taked: the abo blood group test card comprises: the blood group card; The transparent non-setting adhesive film; Drying agent and aluminium pool bag.The blood group card is made of carrier part and dry antibody membrane, and carrier part is made of mark district, detection zone and recording areas; Distinguish corresponding 3 grooves, the antibody membrane of 3 kinds of dryings is arranged in the groove, be respectively anti-A, anti-B and panagglutinin.The transparent non-setting adhesive film is used to cover and detects blood group card mark district and the detection zone that finishes, and reserves part in blood group card upper end and be used to paste archives.Blood group card, transparent non-setting adhesive film and the drying agent aluminium pool bag of packing into constitutes a detection kit.
The utility model prepares a kind of blood type test card and kit comprises as lower member:
1, blood group card
The blood group card is made up of the flat carrier and the dry antibody membrane (comprising anti-A, anti-B and anti-H) of band indenture.Totally 3 of the indentures of carrier are labeled as anti-A, anti-B and contrast respectively.Dry antibody membrane is arranged in indenture, and anti-A, anti-B and panagglutinin are corresponding with anti-A, anti-B and contrast respectively.
2, transparent non-setting adhesive film
Transparent non-setting adhesive film and blood group card are with wide, and test finishes, and after room temperature was done bath, the transparent non-setting adhesive film was overlying on the surveyed area of blood group card, is used for secluding air, have increased the time that the result preserves, and have prevented the bloodborne pathogens pollution operating personnel and the personnel that consult the files simultaneously.Transparent non-setting adhesive is reserved part in blood group card upper end and the blood group card directly can be sticked on the archival paper.
3, drying agent
Decide the consumption of drying agent according to the quantity of the blood group card of packing in aluminium pool bag, drying agent be a bag shape.
4, aluminium pool bag
Decide the size of aluminium pool bag according to the quantity of the blood group card of packing in the aluminium bag.
New novel advantage and the range of application of this practicality:
The utility model is compared with the blood group card that detected abo blood group in the past has following advantage: (1) result is accurate, can effectively control the effect phase of kit, has avoided the false negative that lost efficacy and cause because of antibody with the anti-H of contrast; (2 have made things convenient for the layman to detect blood group, how to judge positive findings with the indication of contrast panagglutinin; (3) transparent non-setting adhesive is reserved in blood group card upper part, the result is preserved and files conveniently; (4) transparent non-setting adhesive has prevented the bloodborne pathogens pollution operating personnel and the personnel that consult the files.(5) room temperature preservation, single part of mensuration, can carry.
The utility model belongs to biomedical sector, and the abo blood group that is mainly used in blood detects.Applying unit of the present utility model is mainly hospital, army, department of public security organs, sportsman's drug-testing department etc., and the individual also can carry out the blood group self check.
Description of drawings:
The structural representation of Fig. 1, blood group card;
The use figure of Fig. 2, transparent non-setting adhesive film.
The blood group card is made up of flat carrier 1 and dry antibody membrane (comprising anti-A8, anti-B and anti-panagglutinin).Carrier is divided into mark district 2, detection zone 3 and recording areas 4.Anti-A is arranged on the tab area, and anti-B and contrasting marking have indenture 5,6 and 7 in the indenture district 3, and recording areas 4 is used to write down person under inspection and tester's dwelling and testing result.
The mark district is arranged on the blood group card flat carrier, detect the difference and the record head of district, transparent non-setting adhesive film and blood group card are with wide.After blood group detected and finishes, room temperature was dried or hair dryer dries up.The transparent non-setting adhesive film covers the mark district 2 and the detection zone 3 of blood group card, and the top redundance directly sticks on the archival paper.
Embodiment:
The preparation of blood type test card and kit and use:
Present embodiment is divided into 5 parts: (1) blood group card flat carrier structure; (2) the dry antibody membrane of preparation; (3) assembling kit; (4) detection and result judge
(1) blood group card flat carrier structure;
Blood group card flat carrier is the scraps of paper of the covering glued membrane of 75 * 75mm, and mark district literal is positioned at carrier upper end 5mm, and detection zone is the indenture of 3 diameter 25mm, and mark is distinguished the anti-A of literal respectively, and anti-B and contrast are corresponding.Recording areas is positioned at the detection zone below, is used to write down name, detects the date, testing result and tester.
(2) the dry antibody membrane of preparation:
Corresponding to the anti-A of literal, anti-B and contrast drip the anti-A of 100ul, anti-B and panagglutinin, lyophilization in blood group card flat carrier detection zone indenture.
(3) assembling kit:
Blood group snaps fits into the aluminium pool bag of 85 * 65mm, reinstalls the packed silica-gel desiccant of 2 grams, and capper seals.
(4) detection and result judge:
Flow process 1: get 5% red cell suspension 1-2 and drip, drip respectively, in B and the contrast indenture,, tilt up and down, note not allowing liquid overflow with toothpick mixing gently in A.Observations within 2 minutes.
Flow process 2: water intaking 1-2 drips, and drips respectively in A, in B and the contrast indenture, with toothpick mixing gently, drips the 3-5ul whole blood more respectively, tilts up and down, notes not allowing liquid overflow.Observations within 2 minutes.
The result judges: occur aggegation positive (+) in the indenture, aggegation negative () do not occur, the judgement of blood group sees the following form.
| The A type | Type B | The O type | The AB type |
Anti-A | + | - | - | + |
Anti-B | - | + | - | + |
Contrast | + | + | + | + |