CN208255066U - A kind of visualization test strips detecting free amino acid in food liquid or drug - Google Patents
A kind of visualization test strips detecting free amino acid in food liquid or drug Download PDFInfo
- Publication number
- CN208255066U CN208255066U CN201820776173.6U CN201820776173U CN208255066U CN 208255066 U CN208255066 U CN 208255066U CN 201820776173 U CN201820776173 U CN 201820776173U CN 208255066 U CN208255066 U CN 208255066U
- Authority
- CN
- China
- Prior art keywords
- test strips
- amino acid
- drug
- free amino
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 title claims abstract description 20
- 239000003814 drug Substances 0.000 title claims abstract description 14
- 235000013305 food Nutrition 0.000 title claims abstract description 14
- 229940079593 drug Drugs 0.000 title claims abstract description 12
- 238000012800 visualization Methods 0.000 title claims abstract description 7
- 238000001514 detection method Methods 0.000 claims abstract description 15
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 abstract description 9
- 235000013336 milk Nutrition 0.000 abstract description 5
- 239000008267 milk Substances 0.000 abstract description 5
- 210000004080 milk Anatomy 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 235000012907 honey Nutrition 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 235000001014 amino acid Nutrition 0.000 description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of visualization test strips detecting free amino acid in food liquid or drug, are related to test strips.For test strips, the test strips use filter paper material, and the width 1 of test strips is 1cm, and length 2 is 5cm, and test strips contain ninhydrin.The test strips colour developing color depends on the acid-base property of test substance.It can be using the method for the samples amino acid contents such as above-mentioned test strips half-quantitative detection milk, honey, Kangfuxin Liquid.Test strips have the advantages that easy to operate, high sensitivity, it is at low cost, be suitble to a variety of food liquids or drug to use, test strips detect easy, quick, intuitive, applied widely, at low cost, the easy popularization and use of method of free amino acid.
Description
Technical field
The utility model relates to test strips, more particularly, to can be used for amino acid in the samples such as milk, fruit juice, Kangfuxin Liquid
The visualization test strips of free amino acid in a kind of detection food liquid or drug of half-quantitative detection.
Background technique
Amino acid is living matter important in organism, is the basic unit of constitutive enzyme and protein.As small molecule,
Amino acid plays particularly important effect to the activity and its physiological function of large biological molecule；As ligand, it can be with a variety of gold
Belong to ion coordination, plays pharmacological function.Therefore, the content for quickly and easily understanding free amino acid in food or drug has weight
The meaning wanted.Currently, the detection method of amino acid content is mainly instrumental method, need to complete analysis detection by hardware
Purpose, and food and medicine common in life, its amino acid content can substantially be understood by not needing instrument there is no one
Horizontal conveniently detection method.
Summary of the invention
The purpose of the utility model is to provide the actual samples that can be used for liquid, and heat treatment can develop the color after contact, face
The height of the depth represented amino acid content of color can substantially be understood by comparing the standard coloration test paper of various concentration gradient
The amino acid content of institute's sample is horizontal, has many advantages, such as a kind of at low cost, easy to operate, high sensitivity detection food liquid
Or in drug free amino acid visualization test strips.
The utility model is test strips, and the test strips use filter paper material, and the area of test strips is 1cm × 5cm, test paper
Item contains ninhydrin, and the molecular structural formula of the ninhydrin is as follows:
The test strips colour developing color depends on the acid-base property of test substance.
It is described can be with the free aminoacid content in half-quantitative detection liquid matrix (including food and drug).
Following methods preparation can be used in the utility model:
(1) the filter paper item of certain area is prepared；
(2) 0.2% ninhydrin ethanol reagent is prepared；
(3) filter paper item is immersed in ninhydrin ethanol reagent, it is dry, it is spare.
The utility model can with the free aminoacid content in half-quantitative detection liquid matrix (including food and drug),
Another purpose of the utility model is to provide a kind of above-mentioned test strips half-quantitative detection milk of application, honey, health
The method of the samples amino acid contents such as multiple new liquid, comprising the following steps:
(1) liquid sample is added dropwise in the test strips, heat treatment is carried out in certain temperature range to develop the color；
(2) colorimetric is carried out with standard coloration test strips after test strips colour developing, substantially may determine that institute's sample amino acid contains
Amount is horizontal.
The test strips of the utility model have easy to operate, high sensitivity, it is at low cost, be suitble to a variety of food liquids or medicine
Product use the advantages of, the utility model test strips detect free amino acid method it is easy, it is quick, intuitive, applied widely, at
This low, easy popularization and use.
Detailed description of the invention
Fig. 1 is the structure composition figure of the utility model embodiment.
Specific embodiment
The utility model is further described below with reference to specific embodiment.
Referring to Fig. 1, the utility model embodiment is test strips, and the test strips use filter paper material, the width 1 of test strips
For 1cm, length 2 is 5cm, and test strips contain ninhydrin.
The test strips colour developing color depends on the acid-base property of test substance.
It is described can be with the free aminoacid content in half-quantitative detection liquid matrix (including food and drug).
Embodiment 1 detects the preparation of the visual test strips of free amino acid in food liquid or drug
1) prepare ninhydrin reagent；
2) prepare the test strips of 1cm × 5cm area, the thickness of test strips can be 0.2cm.
3) filter paper item is immersed in ninhydrin reagent, it is dry, it is spare.
Substep narration in detail below:
1. the preparation of ninhydrin reagent: weighing 0.2g ninhydrin (analysis is pure), be dissolved in 20mL ethyl alcohol, with water constant volume
To 100mL.
2. the preparation of test strips: using scissors by filter paper be cut into area be 1cm × 5cm filter paper item it is several.
3. prepared by test strips: the filter paper item in step 2 is immersed in the ninhydrin reagent in step 1, dry, sealing,
Shading storage.
The preparation of 2 standard coloration test paper of embodiment
1) the filter paper item of 1 step 3) of Example preparation is several；
2) the alanine standard solution of series of concentrations gradient is prepared；
3) above-mentioned steps 1 are successively taken) in each 30 μ L of standard solution, be added drop-wise to 1 step 3) of embodiment preparation filter paper
In item, 90 DEG C of baking oven 15min are placed in, so far, complete the preparation of standard coloration test paper.
3 sample detection embodiment of embodiment
It takes the sample drops such as isometric milk, fruit juice, Kangfuxin Liquid to be added in visualization test strips, is heated in baking oven
(if not having oven conditions, can optionally be carried out using modes such as vapor heating, heating time depends on heating method, with
Develop the color entirely as the judgment criteria for terminating heating), every kind of sample, three times in parallel.Test paper and standard coloration test paper after colour developing
Comparison can determine that the sample amino acid content is horizontal.
Color mechanism of the utility model based on a-amino acid and ninhydrin, the semidefinite applied to amino acid in liquid matrix
Amount detects, and free aminoacid content is higher in matrix, and colour developing degree is deeper.It is above-mentioned that the utility model additionally provides a kind of application
The method for visualizing free amino acid in the samples such as test strips detection milk, honey, Kangfuxin Liquid.It is provided by the utility model
Test strips have the characteristics that easy to operate, high sensitivity, quickly detection, at low cost, the screening of suitable great amount of samples and scene prison
Control.
Claims (1)
1. the visualization test strips of free amino acid in a kind of detection food liquid or drug, it is characterised in that be test strips, institute
Test strips are stated using filter paper material, the width of test strips is 1cm, and length 5cm, test strips are containing ninhydrin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820776173.6U CN208255066U (en) | 2018-05-23 | 2018-05-23 | A kind of visualization test strips detecting free amino acid in food liquid or drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820776173.6U CN208255066U (en) | 2018-05-23 | 2018-05-23 | A kind of visualization test strips detecting free amino acid in food liquid or drug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208255066U true CN208255066U (en) | 2018-12-18 |
Family
ID=66481195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820776173.6U Expired - Fee Related CN208255066U (en) | 2018-05-23 | 2018-05-23 | A kind of visualization test strips detecting free amino acid in food liquid or drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208255066U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110967305A (en) * | 2019-11-25 | 2020-04-07 | 重庆工商大学 | Test paper box for rapidly detecting compost maturity and using method thereof |
-
2018
- 2018-05-23 CN CN201820776173.6U patent/CN208255066U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110967305A (en) * | 2019-11-25 | 2020-04-07 | 重庆工商大学 | Test paper box for rapidly detecting compost maturity and using method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181218 |