CN2608501Y - Expansion bed absorption separator - Google Patents
Expansion bed absorption separator Download PDFInfo
- Publication number
- CN2608501Y CN2608501Y CN 03230239 CN03230239U CN2608501Y CN 2608501 Y CN2608501 Y CN 2608501Y CN 03230239 CN03230239 CN 03230239 CN 03230239 U CN03230239 U CN 03230239U CN 2608501 Y CN2608501 Y CN 2608501Y
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- China
- Prior art keywords
- liquid
- grid distributor
- bed
- protein separation
- separator
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- Expired - Fee Related
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- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A protein separated purification device can directly capture the aim production from the fermented liquid and cell plasm, collect the solid and liquid separating, concentrating and initial purification together. The device is characterized in that a fluid bed is provided processing the liquid containing suspended grains, the device has the advantages of fixed bed strongpoint, flowing to approach the piston flow, low return mixed extent, high separated efficiency, improved receiving rate, reduced investment costs and short operation time.
Description
Affiliated technical field
The utility model relates to a kind of protein separation equipment, relates in particular to a kind ofly can directly catch target product from fermented liquid or cell homogenates liquid, integrates solid-liquid separation, concentrates and the equipment of preliminary purification.
Background technology
At present, conventional liquid phase adsorption post is mainly taked the fixed bed mode, and promptly feed liquid all from the velocity distribution device on the post top chromatography media layer of flowing through, flows out from the post bottom, and fluid is plug flow basically in medium layer, and back-mixing is little, and post is imitated high.Contain the particle feed liquid but can't handle, because the space between this mode medium is little, particle is trapped between medium, results in blockage, and bed pressure drop increases, and absorption can't be carried out.Though fluidized-bed can directly adsorb and contain the particulate feed liquid, there is serious back-mixing problem.
Summary of the invention
In order to overcome the existing separating device deficiency of separate targets product from fermented liquid or cell homogenates effectively, the utility model provides a kind of protein separation equipment.It can directly catch target product from fermented liquid or cell homogenates liquid, collect solid-liquid separation, concentrate and preliminary purification in an operation.
The technical scheme that its technical problem that solves the utility model adopts is: it mainly comprises the glass column that adsorption medium is housed, tuner, cover plate up and down, compositions such as liquid exit hole and feed inlet and outlet.Its main parts are tuners, and it comprises interior external stainless-steel cover pipe, fluid distributor, sealed silicon cushion rubber and screen cloth up and down.
Accompanying drawing 1 is the structural representation of Expanded Bed Adsorption separator.
Accompanying drawing 2 is one of Expanded Bed Adsorption separator embodiment.
Accompanying drawing 3 is an Expanded Bed Adsorption separator bottom grid distributor synoptic diagram.
Accompanying drawing 4 is an Expanded Bed Adsorption separator bottom grid distributor side-view.
Describe preferred forms of the present utility model and embodiment in detail below in conjunction with accompanying drawing.
The utility model is on the basis of fluidized-bed and fixed bed, adopts special fluid distributor, the Expanded Bed Adsorption matrix imported and exported, and tuner is made, and tuner can rely on hydraulic set to move up and down according to different situations.The Expanded Bed Adsorption separator is mainly by glass column 5, upper and lower cover plate 4,12, and tuner 6 and adsorbing base 10 are formed.Tuner by interior external stainless-steel cover pipe 1,2, seals with silica gel circle 7, last fluid distributor 8, and stainless steel mesh 9, upper cover plate 4 and liquid exit hole 3 are formed.Up and down two sealing silica gel circles are all arranged, system sealing when guaranteeing that tuner moves up and down in last fluid distributor and the upper cover plate.Because the Expanded Bed Adsorption separator pressure-drop is very little, therefore its entrance effect is fairly obvious, for guaranteeing that fluid passes through bed with the form of approximate plug flow, so in the entry design of Expanded Bed Adsorption separator a fluid separator 11, the velocity flow profile that can guarantee the cross section is even, reduces because the channel that the uneven distribution of feed liquid causes and other are irregular mobile as far as possible.It allows the interior solid shape particulate of feed liquid to be added with screen cloth on the fluid distributor, passes through as somatic cells or cell debris, and less medium is then had crown_interception.A fluid distributor of being furnished with screen cloth also has been installed in the exit, upper end.In the expanding bed operating process, tuner can be regulated as required, generally is adjusted to be positioned at the bed top just to reduce dead volume.Composite titanium dioxide macroporous copolymer (GET) is housed as Expanded Bed Adsorption matrix in the Expanded Bed Adsorption separator.
The utility model compared with prior art, in the protein separation process, can be directly from zymotic fluid or cell homogenates liquid Middle target acquisition product, the collection Separation of Solid and Liquid concentrates with preliminary purification in an operation, has reduced operating procedure, has shortened behaviour Do the time, saved production cost, owing to adopted the composite titanium dioxide macroporous copolymer as the Matrices for Expanded Bed Adsorption sample flow The whole bed body that evenly distributes, target product absorption evenly penetrate littlely, and rate of recovery height, thereby reduced cost is similar to fixing Bed adsorptivity chromatography. Can be widely used in genetic engineering, enzyme engineering, cell engineering, microbial fermentation engineering and protein engineering etc. The separation and purification process of downstream product in.
Claims (2)
1. protein separation equipment, it mainly comprises cylinder, post import grid distributor and column outlet grid distributor three parts that can rely on hydraulic set to move up and down constitute, post import grid distributor is the porous plate of particular design, solid particulate passes through in these apertures permission treatment solutions, during shut-down operation, can not allow absorbent particles fall.
2. according to the pure equipment of claim 1 described protein separation, it is characterized in that above-mentioned cylinder inner stuffing can adopt composite titanium dioxide macroporous copolymer (GET), as the adsorbing base of expanding bed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03230239 CN2608501Y (en) | 2003-04-11 | 2003-04-11 | Expansion bed absorption separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03230239 CN2608501Y (en) | 2003-04-11 | 2003-04-11 | Expansion bed absorption separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2608501Y true CN2608501Y (en) | 2004-03-31 |
Family
ID=34165234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03230239 Expired - Fee Related CN2608501Y (en) | 2003-04-11 | 2003-04-11 | Expansion bed absorption separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2608501Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690854B (en) * | 2009-09-22 | 2011-08-31 | 周鑫 | Novel expanded bed |
| CN103463834A (en) * | 2013-09-25 | 2013-12-25 | 浙江师范大学 | Constraint bed adsorption separation technology and method |
| CN104710505A (en) * | 2015-03-13 | 2015-06-17 | 中国科学院兰州化学物理研究所 | Expanded bed adsorption system for protein separation and recovery |
-
2003
- 2003-04-11 CN CN 03230239 patent/CN2608501Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690854B (en) * | 2009-09-22 | 2011-08-31 | 周鑫 | Novel expanded bed |
| CN103463834A (en) * | 2013-09-25 | 2013-12-25 | 浙江师范大学 | Constraint bed adsorption separation technology and method |
| CN103463834B (en) * | 2013-09-25 | 2016-03-23 | 浙江师范大学 | A kind of restraining bed adsorption separating method |
| CN104710505A (en) * | 2015-03-13 | 2015-06-17 | 中国科学院兰州化学物理研究所 | Expanded bed adsorption system for protein separation and recovery |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |