CN104710505A - Expanded bed adsorption system for protein separation and recovery - Google Patents
Expanded bed adsorption system for protein separation and recovery Download PDFInfo
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- CN104710505A CN104710505A CN201510110650.6A CN201510110650A CN104710505A CN 104710505 A CN104710505 A CN 104710505A CN 201510110650 A CN201510110650 A CN 201510110650A CN 104710505 A CN104710505 A CN 104710505A
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Abstract
The invention discloses an expanded bed adsorption system for protein separation and recovery, aims to separate and recover protein in starch processing separated juice generated when potatoes or other agricultural products are processed into starch, and belongs to the field of chemical engineering equipment. A four-way ball valve is utilized for controlling the feeding switchover, between the bottom and the top of a chromatographic column, of various liquid materials, so that such processes in protein separation and recovery technologies as material filling and loading in the expanded bed adsorption system, balancing, feeding, cleaning, elution and regeneration can be realized, the defect in the prior art that pipelines need to be dismantled or utilizing more valves to achieve the flow path switchover of the liquid materials in an operation process can be overcome, that is, the structure and the operation of the expanded bed adsorption system are simplified, and the efficiency is improved; according to an expanded bed adsorption technology, the three steps of particle separation, clarification and concentration are all operated in one unit, so that particles in a liquid raw material need not be separated in advance, and the protein recovery technology is simplified.
Description
Technical field
The invention belongs to technical field of chemical, relate to a kind of protein separation and reclaim Expanded Bed Adsorption system, be mainly used in the Separation and Recovery that starch processing that the processings of farm products such as potato, sweet potato, cassava, rice, corn, Chinese sorghum, wheat, pea become starch to produce is separated protein in juice, also can be used for the separation of microorganism fluid fermentation production of protein matter.
Background technology
The processings of farm products such as potato, sweet potato, cassava, rice, corn, Chinese sorghum, wheat, pea become the starch processing produced during starch to be separated the nutritive substances such as juice rich in proteins, and any discharge being separated juice constitutes significant damage to the environmental pollution of processing enterprise location and agricultural production security.If be separated juice to these to adopt the process of routine biochemistry method, have that investment is large, running cost is high, envrionment temperature is too low, treatment unit not easily starts and the difficult problem such as cannot throughout the year to operate, and directly carries out biochemical treatment and result also in the wasting of resources.If the water protein recovery be separated in juice is carried out the recycling that biochemical treatment can realize starch factory processing byproduct more later, also wastewater treatment difficulty can be reduced.
In order to obtain not only nutrition but also food grade (pharmaceutical grade) native protein of safety from starch factory process water, some new separation purification method of Recent study, as ethanol precipitation, carboxymethyl cellulose complexometry, Expanded Bed Adsorption method and membrane separation process etc.Wherein, Expanded Bed Adsorption and membrane separation technique are the most possible methods realizing being separated from starch processing industrialization recovery natural radioactivity albumen juice.But due in the middle of the process water of starch factory discharge except protein, also containing the abundant polysaccharide such as B starch, fine-fibered, pectin, therefore direct membrane sepn can cause the blocking of fenestra.Carrying out liquid state fermentation by microorganism is also a kind of important method of producing protein.Except protein also has the nutrient raw material of a large amount of cell granulations or cell debris and supply microorganism in liquid state fermentation secondary fermentation liquid, therefore direct membrane sepn also can cause the blocking of fenestra.
Expanded Bed Adsorption (Expand Bed Adsorption) technology is a kind of biochemical industry separating and purifying technology occurred in recent years.Solid-liquid separation, fractionation by adsorption, concentrate etc. is integrated into a process by it, and simplifying purifying process, effectively improve product recovery rate, is a biochemical industry new technology had a extensive future.But, up to the present, not yet have and become the starch processing produced during starch to be separated the Expanded Bed Adsorption system of the Separation and Recovery of protein in juice for processings of farm products such as potatos.
Summary of the invention
The object of the invention is the feature reclaiming the separation of protein problem and microorganism fluid fermented protein in the middle of the process water for starch factory discharge, provide a kind of protein separation to reclaim Expanded Bed Adsorption system.
Protein separation recovery Expanded Bed Adsorption system of the present invention, comprises stock liquid basin, level pad basin, elutriant basin, filler regenerated liquid basin, target protein liquid basin, waste liquor storage tank, Expanded Bed Adsorption device and detecting and controlling system.Wherein stock liquid basin, equilibrating buffer basin are connected with the bottom of Expanded Bed Adsorption device by four way ball valve and pipeline; Filler elutriant basin, regenerated liquid basin are connected with the top of Expanded Bed Adsorption device by four way ball valve and pipeline; Target protein liquid basin, waste liquor storage tank are connected with the bottom of Expanded Bed Adsorption device by four way ball valve and pipeline.Described Expanded Bed Adsorption device comprises chromatography column and adsorption bed, and the outer top of chromatography column is provided with X-over to realize bottom chromatography column and the switching of its top feed.Described detecting and controlling system comprises and is arranged on stock liquid basin, level pad basin, elutriant basin, under meter on filler regenerated liquid basin export pipeline, be arranged on the protein concn detector on target protein liquid basin intake pipeline, and the operation interface system be connected by data line with protein concn detector.
Accordingly, in stock liquid the process for separating and recovering of protein and the working process of above-mentioned Expanded Bed Adsorption system as follows:
1) filler loads: by protein adsorption filler with after tap water cleaning, the chromatography column that wet method loads, and post bed height is 50 ~ 70% of chromatography column height;
2) chromatography column balance: the valve opening level pad basin, pumps into pipeline by the solution in level pad basin; Regulate four way ball valve to make control damping fluid from the bottom feed of chromatography column, top is flowed out.The rotating speed of control pump, makes the post bed height of chromatography column expand;
3) charging: stock liquid is pumped into pipeline, regulates four way ball valve to make stock liquid from the bottom feed of chromatography column.Chromatography column bed is in swelling state;
4) clean: regulate four way ball valve, make the solution in damping fluid basin enter chromatography column from bottom, until the solution of chromatography column reaches balance again;
5) wash-out: cleaned rear standing for some time, the filler natural subsidence in chromatography column, post bed height recovers.Switching four way ball valve makes solution in elutriant basin from chromatography column its top feed, bottom discharge.The target protein liquid target approach protein liquid basin eluted, the monitoring software that protein liquid concentration detector carries there will be protein peak, and protein solution flows out wash-out completely and can stop;
6) regenerate: regulate four way ball valve, make filler regenerated liquid from from chromatography column its top feed, clean the filler in chromatography column, make protein adsorption filler activator so that the recycling of Expanded Bed Adsorption system.
For the ease of controlling, the export pipeline of stock liquid basin, equilibrating buffer basin, filler elutriant basin and regenerated liquid basin is separately installed with valve and pump; At target protein liquid basin, the intake pipeline of waste liquor storage tank is separately installed with valve.
Described detecting and controlling system also comprises and is arranged on target protein liquid basin, the pH meter on intake pipeline, and conductivity measuring instrument, carries out on-line monitoring to the pH value of liquid in pipeline and ionic strength.
The X-over on described chromatography column top can adopt swivel joint, can change the stream of chromatography column and various liquid easily by rotating, and realizes bottom chromatography column and freely the switching of its top feed, while adjustable segmented column bed height.
Hinge structure of the present invention has the following advantages:
1. adopt four way ball valve to control the flow direction of various liquid material, to realize bottom chromatography column and freely the switching of its top feed, avoid in prior art the defect needing in operation to remove pipeline or use more valve to switch to the stream realizing liquid material, simplify structure and the operation of Expanded Bed Adsorption system, improve efficiency;
2. expanding bed technology has advantage particle separation, clarification and concentrated three steps being focused on a unit operation, therefore, does not need the particle in stock liquid to be separated in advance, thus simplifies the recovery process of protein liquid;
3, ExPANDED BED ADSORPTION TECHNIQUE is adopted, only have protein to be optionally adsorbed onto on filler, other materials such as starch, fine-fibered, pectin etc. are not then adsorbed through chromatography column, the protein solution ultrafiltration and concentration eluted, can not cause ultra-filtration membrane hole plug, ultrafiltration can be carried out smoothly.
Accompanying drawing explanation
The structural representation of Fig. 1 Expanded Bed Adsorption system of the present invention.
Embodiment
With reference to Fig. 1, a kind of protein separation recovery Expanded Bed Adsorption system, comprises stock liquid basin 1, level pad basin 2, elutriant basin 3, filler regenerated liquid basin 4, target protein liquid basin 14, waste liquor storage tank 15, Expanded Bed Adsorption device and detecting and controlling system.
Wherein stock liquid basin 1, equilibrating buffer basin 2 are connected with the bottom of Expanded Bed Adsorption device by four way ball valve 8 and pipeline; Filler elutriant basin 3, regenerated liquid basin 4 are connected with the top of Expanded Bed Adsorption device by four way ball valve 8 and pipeline; Target protein liquid basin 14, waste liquor storage tank 15 are connected with the bottom of Expanded Bed Adsorption device by four way ball valve 8 and pipeline.During operation, according to technique needs, by adjustment four way ball valve to control the flow direction of various liquid material, to realize bottom chromatography column and freely the switching of its top feed, thus to realize in the process for separating and recovering of protein the technological processs such as expanding bed system filler loading, balance, charging, cleaning, wash-out and regeneration.
Expanded Bed Adsorption device comprises chromatography column 17 and adsorption bed, the top of chromatography column 17 is provided with swivel joint 9(X-over), can to change easily bottom chromatography column and freely the switching of its top feed by rotating, realize the direction of the stream of chromatography column and various liquid, simultaneously the height of adjustable segmented column bed.Chromatography column 17 is for loading protein adsorption filler 16.
Detecting and controlling system comprises and is arranged on stock liquid basin 1, level pad basin 2, elutriant basin 3, under meter 7 on filler regenerated liquid basin 4 export pipeline, be arranged on the protein concn detector 11 on target protein liquid basin 14 intake pipeline, pH meter 12 and conductivity measuring instrument 13 and the operation interface system 10 be connected by data line, can realize protein concn, to pH value and ionic strength on-line monitoring to chromatographic column effluent liquid by the software of operation interface system 10.
Claims (5)
1. a protein separation reclaims and uses Expanded Bed Adsorption system, it is characterized in that: comprise stock liquid basin (1), level pad basin (2), elutriant basin (3), filler regenerated liquid basin (4), target protein liquid basin (14), waste liquor storage tank (15), Expanded Bed Adsorption device and detecting and controlling system; Wherein stock liquid basin (1), equilibrating buffer basin (2) are connected with the bottom of Expanded Bed Adsorption device by four way ball valve (8) and pipeline; Filler elutriant basin (3), regenerated liquid basin (4) are connected with the top of Expanded Bed Adsorption device by four way ball valve (8) and pipeline; Target protein liquid basin (14), waste liquor storage tank (15) are connected with the bottom of Expanded Bed Adsorption device by four way ball valve (8) and pipeline; Described Expanded Bed Adsorption device comprises chromatography column (18) and adsorption bed, and the top of chromatography column is provided with X-over (9) to realize the switching with its top feed bottom chromatography column; Described detecting and controlling system comprises and is arranged on stock liquid basin (1), level pad basin (2), elutriant basin (3), under meter (7) on filler regenerated liquid basin (4) export pipeline, be arranged on the protein concn detector (11) on target protein liquid basin (14) intake pipeline, and the operation interface system (10) be connected by data line with protein concn detector (11).
2. protein separation reclaims and uses Expanded Bed Adsorption system as claimed in claim 1, it is characterized in that: the export pipeline of described stock liquid basin (1), equilibrating buffer basin (2), filler elutriant basin (3) regenerated liquid basin (4) is separately installed with valve (5) and pump (6).
3. protein separation reclaims and uses Expanded Bed Adsorption system as claimed in claim 1, it is characterized in that: at target protein liquid basin (14), the intake pipeline of waste liquor storage tank (15) is separately installed with valve (5).
4. protein separation reclaims and uses Expanded Bed Adsorption system as claimed in claim 1, it is characterized in that: described detecting and controlling system also comprises and is arranged on pH meter (12) on target protein liquid basin (14) intake pipeline and conductivity measuring instrument (13).
5. protein separation reclaims and uses Expanded Bed Adsorption system as claimed in claim 1, it is characterized in that: the X-over (9) on described chromatography column top is swivel joint.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510110650.6A CN104710505A (en) | 2015-03-13 | 2015-03-13 | Expanded bed adsorption system for protein separation and recovery |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510110650.6A CN104710505A (en) | 2015-03-13 | 2015-03-13 | Expanded bed adsorption system for protein separation and recovery |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117398719A (en) * | 2023-12-15 | 2024-01-16 | 江苏尤里卡生物科技有限公司 | Protein separation device for collecting urine and control system thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2608501Y (en) * | 2003-04-11 | 2004-03-31 | 上海华震科技有限公司 | Expansion bed absorption separator |
| CN101838304A (en) * | 2010-04-20 | 2010-09-22 | 成都英德生物工程有限公司 | Method for absorbing and separating human prothrombin complex by utilizing expansion bed |
| CN102898516A (en) * | 2012-10-26 | 2013-01-30 | 浙江大学 | Method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology |
-
2015
- 2015-03-13 CN CN201510110650.6A patent/CN104710505A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2608501Y (en) * | 2003-04-11 | 2004-03-31 | 上海华震科技有限公司 | Expansion bed absorption separator |
| CN101838304A (en) * | 2010-04-20 | 2010-09-22 | 成都英德生物工程有限公司 | Method for absorbing and separating human prothrombin complex by utilizing expansion bed |
| CN102898516A (en) * | 2012-10-26 | 2013-01-30 | 浙江大学 | Method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology |
Non-Patent Citations (2)
| Title |
|---|
| KNUT O. STRÆTKVERN ETC.: "Expanded bed adsorption for recovery of patatin from crude potato juice", 《BIOSEPARATION》 * |
| 曾凡逵，刘刚: "马铃薯蛋白的分离及氨基酸组成分析", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117398719A (en) * | 2023-12-15 | 2024-01-16 | 江苏尤里卡生物科技有限公司 | Protein separation device for collecting urine and control system thereof |
| CN117398719B (en) * | 2023-12-15 | 2024-03-01 | 江苏尤里卡生物科技有限公司 | Protein separation device for collecting urine and control system thereof |
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Application publication date: 20150617 |