CN2602031Y - Device for preparing high absorbance phycoerythrobilin using pink columnar mastoid alga - Google Patents
Device for preparing high absorbance phycoerythrobilin using pink columnar mastoid alga Download PDFInfo
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- CN2602031Y CN2602031Y CN 03236857 CN03236857U CN2602031Y CN 2602031 Y CN2602031 Y CN 2602031Y CN 03236857 CN03236857 CN 03236857 CN 03236857 U CN03236857 U CN 03236857U CN 2602031 Y CN2602031 Y CN 2602031Y
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Abstract
The utility model discloses a device which uses a galaxaura with pink column shape to produce a rhodophyll with high absorbance, and comprises a first culture groove, a second culture groove, a collector, a grinder, a mixer and a precipitator; the utility model is characterized in that the galaxaura with pink column shape for the culture liquid contains three generation alternative including sexual reproduction, asexual reproduction and vegetative reproduction among mature using the galaxaura living history with pink column shape, the carpospores filamentous form phase does not contain any gum; under condition of controlled light, temperature and nutrition condition, the filamentous form phase continues and furthermore is used as raw material to extract the rhodophyll with high absorbance ratio. Through high weight ratio of the rhodophyll contained in unit weight, effect of lowering unit cost and reducing purifying step of rhodophyll has been reached.
Description
Technical field
The utility model relates to the device that has high absorbance (OD) ratio phycoerythrobilin with pink columnar galaxaura (Galaxauraoblongata) production.Utilize syngenesis, the monogony that pink columnar galaxaura has in the life history and nourish and generate three digenesis and carpospores filament stage do not contain under the feature of various glue classes, under light, temperature and the nutritional condition of control, continue its filament stage, and be material with it further, extraction high absorbance ratio phycoerythrobilin.
Background technology
No matter with regard to phycoerythrobilin, algocyan or other natural protein pigment, usually has practical security, heat, potential of hydrogen etc. are also had the stability of certain degree, can be applicable on food and the makeup, the while also can be used as the fluorochrome that the antibody sign is used on the immunology and is applied in clinical diagnosis and the cellular biochemical research.
Existing market has been developed and used algocyan (phycocyanin) and phycoerythrobilin (phycoerythrin), wherein, the raw material sources of algocyan production mostly are and can give the blue-green algae that a large amount of breedings are cultivated, as spirulina (Spirulina) and patina Microcystis aeruginosa (Microcystis), and it is cultivated and production equipment is enjoyed patent more.
Phycoerythrobilin is then because of the shortage of raw material, or be unfavorable for pigment production because of former material, and exist output to reach expensive defective less, demand to the edible red pigments of the annual tens thousand of pounds in the whole world, must develop the red pigments that a kind of natural, safe, stable special fluorescence of tool again disengages, after estimating mass production, price will be descended, and more can enlarge its range of application and the market supply.
Algae haematochrome albumen major part is the large-scale thallus of separating from red algae at present, and as laver (Porphyra) or rosetangle algae (Ceramium) etc., small part then extracts from the Porphyridium cruentum (Porphyridium) that can control cultivation in a large number.Its subject matter is:
Though 1, the laver of wild a large amount of red algae resource and cultivation is arranged as the phycoerythrobilin raw material at present, but, most red algaes enrich colloid (agar glue, carrageeman, Furcellaria gum) because of containing, cause the collection of pigment to go out difficult, especially dried algae raw material is all the more so, the raw material of adding wild algae kind all has seasonal variation on product and output, by manpower is difficult for grasping.
2, at present Porphyridium cruentum be incubated at collecting cell the time, significant difficulty is also arranged, because the collection of unicellular frond one to be the power consumption and operation consuming time, it is very big to influence production cost, and frustule secreted solubility Polysaccharides when cultivating, except the collection of block cell, also have influence on the extraction of pigment.
In order to solve the above problems, United States Patent (USP) 5,358,858 have proposed a kind of method of producing phycoerythrobilin by flagelliform nostoc (Bangiaat ropurpurea) and narrow leaf laver (Porphyraangusta) filament, utilize flagelliform nostoc and the thread sporophyte stage of narrow leaf laver in the life history not to contain under the feature of various glue classes, utilize under light, temperature and the nutritional condition of control, continue its filament stage, and be raw material with it further, the extraction phycoerythrobilin, its step is as follows:
1, the cultivation of filament and breeding, from sophisticated flagelliform nostoc of field acquisition or narrow leaf laver gametophyte, it is clean to clean frond with sterilization seawater and writing brush, the back input of drying in the shade slightly contains in SWM-III (as shown in Table 1) culture dish of improveing, the culture dish inner bottom part is covered with cover glass, after treating that spore discharges and is attached to cover glass, at room temperature, the illumination of 1000-4000lux and every day 10-16 hour irradiation incubator in germinate and grow up, grow at last and be the highly branched thread sporophyte of difference.
The composition of table one SWM-III
NaNO
38.5g/100ml get 2ml
Na
2HPO
40.595g/100ml get 2ml
Na
2EDTA 0.5g/100ml gets 2ml
0.01625g/100ml get 2ml
FeCl
3(or FeCl
37H
2O) 0.02885g/100ml gets 2ml
*
1PI-metal gets 2ml
*
2S-3Vitamins gets 2ml
Soilextract gets 50ml
Tris (10cc/l) gets 500mg
Livere xtract gets 10mg
Seawater pH=7,51 liters
(when transferring pH: transfer to pH≤7 with dense HCl earlier, the NaOH with proper concn transfers to pH7,5 again, produces white precipitate in the time of so can avoiding sterilizing)
* wherein
1PI-metal is:
H
3BO
3 12.368g
MnCl
2 1.385g
ZnCl
2 0.109g
2 liters of distilled water
CoCl
2·6H
2O 4.479mg
CuCl
2·2H
2O 0.034mg
* wherein
2S-3Vitamins is:
ThiaminHCl 0.5g
Capantothenate 0.1g
Nicotinicacid 0.1g
P-aminobenzoicacid 10mg
Biotin 1mg
2 liters of distilled water
Inositol 5g
Folicacid 2mg
Thymine 3mg
B
12 1mg
(all Stock solution all is stored in the brown bottle, stored refrigerated)
2, move to the triangular flask that contains nutrient solution after filament scrapes, under above-mentioned growing environment condition, cultivate, breed the agglomerated masses of filament.Smash the agglomerated masses of filament, the measuring bottle that moves to bigger growth continues to develop into more agglomerated masses, and the therefore volume of enlarged culturing gradually, and (300 milliliters of cleaned airs of per minute) a large amount of breedings of in the irradiation culture tank of comparatively large vol, should inflating are cultivated.Screen cloth by the 100-400 mesh filters, and the nutrient solution of filtering is recyclable to be re-used, and follow-up cultivation be there is no obstruction.
3, after the frond results, can be dry rapidly because of filar structure through vacuum or warm wind, after pulverizing again, fully stir extraction, centrifugal with water or phosphate solution, can get clarifying dark red algae protein solution; Concentrating and purifying of phycochrome can be removed the partial impurities albumen precipitation by 20% ammoniumsulphate soln, is settled out chromoprotein through the 60-65% ammoniumsulphate soln again in solution, is OD
565/ OD
280The thick red pigments of=1.4-1.6 is food grade, makeup available pigment.
4, sedimentary albumen is further analysed purifying (gelfiltration) by the glue filtering layer, use Sephadex G200 resin to separate, the first road program can get OD than being the phycoerythrobilin product of 3.3-3.7, repeat once more to get final product OD than being the phycoerythrobilin of 5.1-5.2, show that through the result of colloid electrophoresis method (SDS) electrophoresis gained purity is about 99% again, can be used as immunity inspection reagent.
Though above-mentioned method can avoid traditional laver, rosetangle algae extraction pigment method to need the complicated formality with heating and separation gel value, or one-celled plants extraction process such as Porphyridium cruentum, because its volume is little, the problem that causes the difficulty of collecting and its excretory Polysaccharides to influence the extraction of pigment, its major defect is:
But the dark red algae protein solution that flagelliform nostoc and narrow leaf conchocelis of porphyra directly form, its OD
565/ OD
280Have only 1.4-1.6, still need carry out concentrating and purifying of phycochrome, obtain the phycoerythrobilin of high OD value, cause processing procedure complexity and cost higher.Therefore we need develop the filament of other algae kind, the phycoerythrobilin that makes its first dark red algae protein solution that forms just have high OD value.
In above-mentioned background technology, the dark red algae protein solution that tradition utilizes flagelliform nostoc and narrow leaf conchocelis of porphyra directly to form, the problem that its absorbance ratio is on the low side.The utility model people creates the device that utilizes pink columnar galaxaura to produce phycoerythrobilin, avoids said circumstances to produce.
Summary of the invention
Main purpose of the present utility model provides the device that the pink columnar galaxaura of a kind of usefulness is produced the high absorbance phycoerythrobilin, contains the high weight ratio of phycoerythrobilin by its unit weight, reaches the purpose that reduces unit cost.
Another purpose of the present utility model provides the device that the pink columnar galaxaura of a kind of usefulness is produced the high absorbance phycoerythrobilin, can extract by it to have high absorbance ratio phycoerythrobilin, reaches the purpose that reduces the phycoerythrobilin purification step.
The purpose of this utility model is achieved in that a kind of device that utilizes pink columnar galaxaura to produce the high absorbance phycoerythrobilin, it is to use the filament of the carpospores that has syngenesis, monogony the life history and nourish and generate three heterogamous algae kinds to produce the device of the phycoerythrobilin of high absorbance ratio, and it is characterized in that: it includes first culture tank, second culture tank, collector, mill, agitator and settling vessel;
First culture tank with nutrient solution is cultivated pink columnar galaxaura and is contained the sporangial gametophyte of mellow fruit, and obtains carpospores;
This second culture tank closes on this first culture tank, and illumination, light with 500-6000lux are more than 10: 14, and the environment under 15-30 ℃, cultivates this carpospores and grows into filament; The collector of collecting this filament places this second culture tank; The mill that grinds this filament closes on this collector;
This agitator closes on this mill, and this filament after adding acid-base buffer and grinding obtains a dark chromoprotein solution;
This settling vessel closes on this agitator, in order to go out phycoerythrobilin from this dark color chromoprotein solution precipitation.
The phycoerythrobilin that this pink columnar galaxaura extracts from its carpospores filament, the chromatography spectrogram of the 565nm that is drawn through the liquid chromatography (LC) instrument.This nutrient solution is the SWM-III nutrient solution.This SWM-III nutrient solution is for removing organic SWM-III nutrient solution.The method of cultivating this carpospores filament is for revolving floating culture method.The environment of this cultivation is 20 ℃, and the illumination of 2000lux and light are 12: 12.The collector of collecting this filament is a screen cloth.This screen cloth is the screen cloth of 20-400 mesh.This mill more includes moisture eliminator.This moisture eliminator is a vacuum drier.This moisture eliminator is warm wind moisture eliminator.The settling vessel that precipitates this phycoerythrobilin is the salt analysis apparatus.Be provided with the ammoniumsulphate soln of 60-65% in this salt analysis apparatus.It also includes purifier, and this purifier is a glue filtering layer analysis apparatus.This glue filtering layer analysis apparatus is for using the glue filtering layer analysis apparatus of Sephadex G200.It also includes purifier, and this purifier is a ultrafiltration unit.
Describe in detail below in conjunction with preferred embodiment and accompanying drawing.
Description of drawings
Fig. 1 is that the phycoerythrobilin UV that utilizes high-effect liquid chromatography (LC) instrument (HPLC) to draw absorbs and fluorescent emission chromatography spectrogram;
Fig. 2 is the flagelliform nostoc synoptic diagram of the life history;
Fig. 3 is the narrow leaf laver synoptic diagram of the life history;
Fig. 4 is the sea synoptic diagram of the life history;
Fig. 5-Fig. 7 is the 280nm, the 565nm that are drawn through high-effect liquid chromatography (LC) instrument of pure matter phycoerythrobilin that traditional flagelliform nostoc extracts, the chromatography spectrogram of 615nm.
Fig. 8-Figure 10 is the 280nm, the 565nm that are drawn through high-effect liquid chromatography (LC) instrument of pure matter phycoerythrobilin that traditional narrow leaf laver extracts, the chromatography spectrogram of 615nm.
Figure 11-Figure 13 is the 280nm, the 565nm that are drawn through high-effect liquid chromatography (LC) instrument of pure matter phycoerythrobilin that pink columnar galaxaura of the present utility model extracts, the chromatography spectrogram of 615nm.
Figure 14-Figure 18 is that the utility model utilizes pink columnar galaxaura to produce the device and the using method synoptic diagram thereof of high absorbance phycoerythrobilin.
Embodiment
Following preferred embodiment only is explanation the utility model, can also implement at the embodiment of other algae kind widely, and scope of the present utility model is not subjected to the qualification of following preferred embodiment.
As everyone knows, phycobiliprotein is from the intravital a kind of water soluble fluorescence proteinochrome of algae.Because of it has special photoluminescent property, mainly applied to widely on the fluorescent reagent of wandering cells instrument at present.In the various phycobiliprotein, phycoerythrobilin is wherein a kind of, also is the highest a kind of of fluorescence intensity in the natural goods, therefore has many fluorescence detection devices to adopt this type of pigment.
Fig. 1 UV that high-effect liquid chromatography (LC) instrument (HPLC) draws for phycoerythrobilin utilizes absorbs and fluorescent emission chromatography spectrogram, and its chromatography condition is as described below:
HPLC column：HYDROCELL DEAE NP10
Column size：50*4.6mm
Buffer A：10mMK-PBS pH6.0
Buffer B：10mMk-PBS，0.5M NaCl pH6.0
Gradient：0％Buffer B→12min→50％Buffer B
Detection：565nm
Flow rate：1ml/min
And high-effect liquid chromatography (LC) instrument mainly is made up of the high degree of accuracy high pressure pump housing, separator tube, detecting instrument and register.
Consult shown in Figure 2, viewing head is delivered vegetables the life history, syngenesis is fertilized after 4 with the sperm 3 that antheridium 2 (being male mating partner 1) discharges for producing archicarp 6 (being female mating partner 5), can develop into carpospores 8 at carpospore cyst 7, after carpospores 8 maturations of the inside, promptly can shed, become thread sporophyte 9, under side waist shape chromatoplast b effect, grow into sporozoite body 10 and shell sporozoite capsule 11, it is emitted shell sporozoite 12 and further grows into the young 13 at unit cell formation position 15, single-row frond 14 is under starlike chromatoplast a effect, form unit cell 16, unit cell 16 further forms upright body (mating partner) 18, multiple row frond 17 is grown into male mating partner 1.
Monogony then is to become to sprout into the young 13, single-row frond 14 and small-sized frond by monospore 16 direct developments, by the time can produce monospore 16 again in the time of certain, monospore 16 is sprouted into single-row frond 14 and small-sized frond again, so circulation is until under the suitable envrionment conditions, single-row frond 14 and the monospore 16 that small-sized frond produced just can be sprouted into large-scale frond 17.Simultaneously, single-row frond 14 and small-sized frond also can be transformed into large-scale frond 17, enter syngenesis more from generation to generation.
Consult shown in Figure 3ly, observe narrow leaf laver in the life history, because its flagelliform nostoc cardinal principle life history with Fig. 2 is identical, so do not describe in detail.We can learn that generally be syngenesis and two digenesis of monogony the life history of above-mentioned two kinds of algae, conventional art is exactly to utilize the thread sporophyte stage not contain the feature of various glue classes, under light, temperature and the nutritional condition of control, continue its filament stage, and be raw material with it further, the extraction phycoerythrobilin.
Consult shown in Figure 4ly, observe sea (Nemalion) life history, because its flagelliform nostoc cardinal principle life history with Fig. 2 is identical, so do not describe in detail.The life history of algae is except being with syngenesis and two digenesis of monogony, the part algae also has more one and nourishes and generates from generation to generation in addition, promptly produce tetrasporic sporophyte (being tetrasporophyte), its process forms carposporophyte (carposporephyte) for the archicarp after fertilization, carpospores (carpospore), tetrasporophyte (tetrasporophyte), tetrasporangium (tetrasporangium), be tetraspore (tetraspore) at last, wherein carpospores all can sprout into filament with tetraspore, but the characteristic difference of these two kinds of filaments.
The utility model is exactly to utilize to have the pink columnar galaxaura from generation to generation of nourishing and generating, its carpospores filament stage does not contain the feature of various glue classes, under light, temperature and the nutritional condition of control, continue its filament stage, and be raw material with it further, extract phycoerythrobilin with high OD value.
Consult Figure 14-shown in Figure 180, device of the present utility model and using method thereof are as follows:
1, the cultivation of filament and breeding, sophisticated pink columnar galaxaura is contained the sporangial gametophyte of mellow fruit, it is clean to clean frond with sterilization seawater and writing brush, after drying in the shade slightly, drop into and do not contain in first incubator 100 of organic SWM-III nutrient solution 102, first incubator, 100 inner bottom parts are covered with sheet glass 103, after treating that carpospores 101 (carpospore) discharges and is attached to sheet glass 103, under 15-30 ℃, the illumination of 500-60001ux, light are more than 10: 14, and every days 10 above irradiation first incubator 100 in the growth of germinateing, growth at last is the highly branched filament of difference, as shown in figure 14.
2, after filament 201 scrapes, move to the large-scale culture tank 200 that contains nutrient solution, under above-mentioned isometric growth envrionment conditions, cultivate, can breed the loose little agglomerated masses of filament, and the therefore volume of enlarged culturing gradually, in the irradiation culture tank of comparatively large vol, the device 202 of should inflating is inflated, and this little agglomerated masses is suspended in (is called and revolves floating the cultivation) a large amount of breeding cultivations in the nutrient solution 204.Screen cloth 203 by the 20-400 mesh filters, and the nutrient solution of filtering is recyclable to be re-used, and follow-up cultivation be there is no obstruction, as shown in figure 15.
3, after frond 303 results, because of filar structure can be dry rapidly through vacuum or warm wind device 301, again after automatic grinding mill 302 is pulverized, get the powder of 2.4 grammes per square metres, add 70 milliliters, potassium phosphate solution or other acid-base buffer 304 of 10mm, fully stir with agitator 303, to keep the pH value of solution value between 5-10, fully stir, again with use whizzer 305 under 6000rpm, 10min, 4 ℃ the state, can get clarifying dark red algae protein solution 306.Concentrating and purifying of phycochrome can be removed partial impurities albumen precipitation 308 by 20% ammoniumsulphate soln 307, is settled out chromoprotein through 60%-65% ammoniumsulphate soln 309 again in solution, and it is OD
565/ OD
280=2.66 thick red pigments 310, it is food grade or makeup available pigment.Above-mentioned clarifying dark red algae protein solution, also can grind with the wet frond of direct results, add the phosphoric acid potassium solution after, get with the whizzer centrifuging and taking again, and concentrating with purifying of phycochrome can be undertaken by ultra-filtration method (ultrafiltration), as Figure 16-shown in Figure 180.
4. sedimentary albumen is further analysed purifying (gel filtration) by the glue filtering layer, use 120 centimeters long Sephadex G200 resin to separate, the first road program can get OD than being phycoerythrobilin product more than 4.5, repeat once more, get final product to such an extent that the OD ratio is 5.3 above phycoerythrobilin, again through colloid electrophoresis method (SDS) electrophoresis, the result of gained shows that purity is about 99%, can be used as immunity inspection reagent.
Consult Fig. 5-shown in Figure 7,280nm, the 565nm that the pure matter phycoerythrobilin that extracts from flagelliform nostoc for tradition is drawn through high-effect liquid chromatography (LC) instrument (HPLC), the chromatography spectrogram of 615nm,
Consult Fig. 8-shown in Figure 10,280nm, the 565nm that the pure matter phycoerythrobilin that extracts from narrow leaf laver for tradition is drawn through high-effect liquid chromatography (LC) instrument, the chromatography spectrogram of 615nm.In fact, the phycoerythrobilin that from different algae kinds, is extracted, it draws also difference to some extent of chromatography spectrogram through high-effect liquid chromatography (LC) instrument, just as the mankind's finger print.Therefore, we can tell phycoerythrobilin by this characteristic and by which kind of algae kind are produced.
Consulting Figure 11-shown in Figure 13, is the 280nm, the 565nm that are drawn through high-effect liquid chromatography (LC) instrument of pure matter phycoerythrobilin that the utility model takes out from pink columnar galaxaura, the chromatography spectrogram of 615nm.
Table two is the comparison sheet of traditional flagelliform nostoc, narrow leaf laver and pink columnar galaxaura extraction phycoerythrobilin of the present utility model.
First unit's of classifying as algae weight (gram) contains the ratio of phycoerythrobilin weight (milligram), representative can output under identical algae weight phycoerythrobilin, we can know and learn: pink columnar galaxaura of the present utility model, its ratio that contains phycoerythrobilin is for the highest.
Second and third classifies the absorbance ratio (OD that extracts phycoerythrobilin from the algae kind as
565/ OD
280) and (OD
615/ OD
565), the former represents the ratio of phycoerythrobilin and other lipidated protein, the latter represents the purity ratio of algocyan and phycoerythrobilin, (OD
565/ OD
280) the high more expression phycoerythrobilin of ratio purity is high more, its value added is high more, has food or cosmetic colors utility value more, purifying is made in also favourable more rear end, because of the immunity inspection that can be applicable to medical treatment with on the reagent; And can absorb the fluorescence that phycoerythrobilin sends because of algocyan, cause conduct fluorescence color when food or cosmetic colors purposes not good, therefore (OD
615/ OD
565) low more expression algocyan content is few more, and is better on the fluorescence color effect.Similarly, we can obviously find out: pink columnar galaxaura of the present utility model, it extracts phycoerythrobilin and has the highest absorbance ratio.Therefore, utilize pink columnar galaxaura filament of the present utility model to extract phycoerythrobilin, it has the advantage of high phycoerythrobilin content and high absorbance ratio than the filament of conventional hair dish and narrow leaf laver.
The plain analysis of components of each algae pink colour of table two
| The algae kind | Ba | Pa | Go |
| RPE mg/g algae powder | 53.5 | 38.89 | 57.74 |
| Extinction ratio A 565/A 280 | 1.40 | 1.54 | 2.66 |
| Extinction ratio A 615/A 565 | 0.19 | 0.53 | 0.14 |
Wherein
RPE: a kind of phycoerythrin, possess hydrophilic property and water with form the stable aqueous solution, its maximum UV absorbing wavelength and fluorescent emission wavelength respectively are 566nm and 575nm.
Ba: conventional hair dish (Bangia atropurpurea)
Pa: traditional narrow leaf laver (Porphyra angusta)
Go: pink columnar galaxaura of the present utility model (Galaxaura oblongata)
The utility model is to describe by enumerating a preferred embodiment; but the utility model is not limited to the embodiment that enumerated; apparently; other does not break away under the spirit that the utility model discloses; the equivalence of being finished changes or modifies, and all should be included within the protection domain of the present utility model.
In sum, embodiment of the present utility model can reach desired effect, and its disclosed concrete structure not only be not seen in like product again, also be not disclosed in application before, have novelty, creativeness and practicality.
Claims (16)
1, a kind of device that utilizes pink columnar galaxaura to produce the high absorbance phycoerythrobilin, it is characterized in that: it includes first culture tank, second culture tank, collector, mill, agitator and settling vessel;
First culture tank with nutrient solution is cultivated pink columnar galaxaura and is contained the sporangial gametophyte of mellow fruit, and obtains carpospores;
This second culture tank closes on this first culture tank, and illumination, light with 500-6000lux are more than 10: 14, and the environment under 15-30 ℃, cultivates this carpospores and grows into filament; The collector of collecting this filament places this second culture tank; The mill that grinds this filament closes on this collector;
This agitator closes on this mill, and this filament after adding acid-base buffer and grinding obtains a dark chromoprotein solution;
This settling vessel closes on this agitator, in order to go out phycoerythrobilin from this dark color chromoprotein solution precipitation.
2, device according to claim 1 is characterized in that: the phycoerythrobilin that this pink columnar galaxaura extracts from its carpospores filament, the chromatography spectrogram of the 565nm that is drawn through the liquid chromatography (LC) instrument.
3, device according to claim 1 is characterized in that: this nutrient solution is the SWM-III nutrient solution.
4, device according to claim 3 is characterized in that: this SWM-III nutrient solution is for removing organic SWM-III nutrient solution.
5, device according to claim 1 is characterized in that: the method for cultivating this carpospores filament is for revolving floating culture method.
6, device according to claim 1 is characterized in that: the environment of this cultivation is 20 ℃, and the illumination of 2000lux and light are 12: 12.
7, device according to claim 1 is characterized in that: the collector of collecting this filament is a screen cloth.
8, device according to claim 7 is characterized in that: this screen cloth is the screen cloth of 20-400 mesh.
9, device according to claim 1 is characterized in that: this mill more includes moisture eliminator.
10, device according to claim 9 is characterized in that: this moisture eliminator is a vacuum drier.
11, device according to claim 9 is characterized in that: this moisture eliminator is warm wind moisture eliminator.
12, according to the described device of claim 1, it is characterized in that: the settling vessel that precipitates this phycoerythrobilin is the salt analysis apparatus.
13, according to the described device of claim 12, it is characterized in that: the ammoniumsulphate soln that is provided with 60-65% in this salt analysis apparatus.
14, device according to claim 1 is characterized in that: it also includes purifier, and this purifier is a glue filtering layer analysis apparatus.
15, according to the described device of claim 14, it is characterized in that: this glue filtering layer analysis apparatus is for using the glue filtering layer analysis apparatus of SephadexG200.
16, according to the described device of claim 1, it is characterized in that: it also includes purifier, and this purifier is a ultrafiltration unit.
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03236857 CN2602031Y (en) | 2003-01-27 | 2003-01-27 | Device for preparing high absorbance phycoerythrobilin using pink columnar mastoid alga |
| AT04703331T ATE406381T1 (en) | 2003-01-27 | 2004-01-20 | METHOD FOR PRODUCING PHYCOERYTHRIN WITH HIGH OPTICAL DENSITY |
| BR0406984-6A BRPI0406984A (en) | 2003-01-27 | 2004-01-20 | Process for the production and use of high optical density phycoerythrin |
| CA002514648A CA2514648A1 (en) | 2003-01-27 | 2004-01-20 | Process and device for preparing phycoerythrin with high od ratio |
| DE602004016117T DE602004016117D1 (en) | 2003-01-27 | 2004-01-20 | PROCESS FOR THE PREPARATION OF HIGH OPTICAL DENSITY PHYCOERYTHRINE |
| EP04703331A EP1591518B1 (en) | 2003-01-27 | 2004-01-20 | Process for preparing phycoerythrin with high optical density |
| PCT/CN2004/000075 WO2004067695A1 (en) | 2003-01-27 | 2004-01-20 | Process and device for preparing phycoerythrin with high od ratio |
| NZ541689A NZ541689A (en) | 2003-01-27 | 2004-01-20 | Process and device for preparing phycoerythrin with high OD ratio |
| KR1020057013884A KR100792608B1 (en) | 2003-01-27 | 2004-01-20 | Process and device for preparing phycoerythrin with high od ratio |
| AU2004207600A AU2004207600A1 (en) | 2003-01-27 | 2004-01-20 | Process and device for preparing phycoerythrin with high OD ratio |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03236857 CN2602031Y (en) | 2003-01-27 | 2003-01-27 | Device for preparing high absorbance phycoerythrobilin using pink columnar mastoid alga |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2602031Y true CN2602031Y (en) | 2004-02-04 |
Family
ID=34167039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03236857 Expired - Lifetime CN2602031Y (en) | 2003-01-27 | 2003-01-27 | Device for preparing high absorbance phycoerythrobilin using pink columnar mastoid alga |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2602031Y (en) |
-
2003
- 2003-01-27 CN CN 03236857 patent/CN2602031Y/en not_active Expired - Lifetime
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