CN106916748A - Chrysophyceae and its cultural method - Google Patents
Chrysophyceae and its cultural method Download PDFInfo
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Abstract
The present invention relates to a kind of chrysophyceae (Poterioochromonas malhamensis), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center with preserving number CGMCC No.11620.The invention further relates to a kind of cultural method of chrysophyceae, including the nutrient source that bran grain and alternatively nutritive salt and vitamin grow as chrysophyceae is gone to add the aqueous system for cultivating chrysophyceae cereal.The cereal is selected from one or more in the following group：Paddy rice, wheat, millet, black rice, buckwheat, oat, myotonin and sorghum, preferably paddy rice and wheat.The nutritive salt and vitamin include NaNO3、KH2PO4、K2HPO4, vitamin B6And vitamin B12.The chrysophyceae algae strain that the present invention is separate is easy to cultivate and grow rapidly.Cultural method used by the present invention is simple, cultivates raw materials used cheap, and toxigenic capacity is greatly lowered, while this method is built upon under nonsterile conditionses, is well suited for large-scale culture application.
Description
Technical field
The application is related to microalgae industry application field, in particular to one plant of new separate chrysophyceae, i.e., Poterioochromonas malhamensis CMBB-01 plants, and its cultural method.
Background technology
Chrysophyceae is also known as golden brown alga, and majority is distributed in fresh water, is also distributed in the seawater.Containing abundant nutrition in the cell of chrysophyceae, wherein chrysolaminaran is the ergastic substances of its cell, and it has extensive BA, has certain preventive and therapeutic effect to gout, high fat of blood, early metaphase kidney failure.Meanwhile, chrysophyceae or a kind of good fish food have been widely used in aquaculture.Additionally, there are some researches show chrysophyceae can be effectively swallowed including the various Bloom-causing Algals including microcystic aeruginosa, therefore also have potential use in body eutrophication improvement.
Traditional chrysophyceae autotrophy cultural method relies primarily on the photosynthesis of chrysophyceae, and chrysophyceae growth is very slow.Additionally, the chrysophyceae species being currently known and algae strain are also unsuitable for heavy industrialization culture.Shortage is easy to the chrysophyceae algae strain of culture and corresponding cultural method is development algae industry institute problem demanding prompt solution.
In view of chrysophyceae the fields such as medicine, environmental protection, cultivation huge economic value, it is necessary to one kind is easy to growth, it is adaptable to the chrysophyceae of industrialization culture, and the method for cultivating it.
The content of the invention
The present invention relates to a kind of new chrysophyceae algae strain being separated to from natural environment, identified Classification And Nomenclature is P.malhamensis CMBB-01 plants.Research shows that chrysophyceae algae strain being capable of fast-growth, it is easy to large-scale culture.
On the one hand, the present invention relates to a kind of CMBB-01 plants of chrysophyceae P.malhamensis, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center with preserving number CGMCC No.11620.
On the other hand, the present invention relates to a kind of method for cultivating chrysophyceae of the invention, including the nutrient source that bran grain grow as chrysophyceae is gone to add the aqueous system for cultivating chrysophyceae cereal, wherein the cereal is selected from one or more in the following group：Paddy rice, wheat, millet, black rice, oat, buckwheat, myotonin and sorghum, preferably paddy rice and wheat.
In one embodiment, methods described further includes to add following nutritive salt and vitamin in chrysophyceae cultivating system：NaNO3、KH2PO4、K2HPO4, vitamin B6And vitamin B12。
In one embodiment, the amount of the grain for being added in the aqueous system is 1-50g/L, more preferably 5-15g/L, in terms of the dry weight of grain.
In one embodiment, when cereal is paddy rice, the amount added is 12.5-50g/L.
In one embodiment, when cereal is wheat, the amount added is 5-10g/L.
In one embodiment, the addition of nutritive salt and vitamin is in every 1L aqueous system：NaNO3:140mg、KH2PO4:10mg、K2HPO4:5mg, vitamin B6:1 μ g and vitamin B12:1μg。
In one embodiment, temperature of the culture at 20-28 DEG C, preferably 22-25 DEG C, 50-100 μm of ol photonsm-2·s-1, preferably 50 μm ol photonsm-2·s-1Luminous intensity, and carried out under the pH of 6.0-8.5.
The chrysophyceae algae strain that the present invention is separate is easy to cultivate, grow rapidly, and contained chrysolaminaran content is very high.Cultural method used by the present invention is simple, cultivates raw materials used cheap, and toxigenic capacity is greatly lowered.Meanwhile, cultivating system is built upon under aseptic condition, is highly suitable for utilization and extention in extensive system.
Brief description of the drawings
Figure 1A：Chrysophyceae of the present invention photo under an optical microscope.
Figure 1B：Photo of the chrysophyceae of the present invention under electronic scanner microscope.
Fig. 2：Chrysophyceae rbcL genes of the present invention carry out the result of BLAST in ncbi database.
Fig. 3：Chrysophyceae 18s rDNA genes of the present invention carry out the result of BLAST in ncbi database.
Fig. 4：Chrysophyceae of the present invention is using removing the rice grain of bran as the growing state under conditions of nutrient source.
Fig. 5：Chrysophyceae of the present invention is using removing the wheat berry of bran as the growing state under conditions of nutrient source.
Fig. 6：Chrysophyceae of the present invention is using removing wheat berry, nutritive salt and the vitamin of bran as the growing state under conditions of nutrient source.
Fig. 7：Growth curve of the chrysophyceae of the present invention under different illumination conditions.
Specific embodiment
The present inventor is unexpectedly separated to one plant of new chrysophyceae, i.e. CMBB-01 plants from natural environment.Morphology and molecular biology identification show its species in belonging to Chrysophyceae (Chrysophyceae).The inventors discovered that CMBB-01 plants has excellent growth performance and chrysolaminaran content very high.
Therefore, first aspect, the invention provides a kind of CMBB-01 plants of chrysophyceae Poterioochromonas malhamensis, it was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center with preserving number CGMCC No.11620 on December 2nd, 2015, and (CGMCC, address is：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica).
Present inventors have further discovered that, in aqueous system, using the nutrient source of the grain of bran as the strain of this chrysophyceae algae is removed, be alternatively aided with appropriate nutritive salt and vitamin, the strain of this chrysophyceae algae can raised growth in a short time, biomass increases substantially.
Therefore, second aspect, cultivates the method for the separated chrysophyceae algae strain of the present invention, including the nutrient source that bran grain grows as chrysophyceae that goes of cereal is added in the aqueous system for cultivating chrysophyceae present invention also offers a kind of.
Term " grain of cereal " is primarily referred to as the seed of grass, and such as grain can be paddy rice, wheat, millet, black rice, buckwheat, oat, myotonin and sorghum.In one embodiment, the cereal used by the present invention is selected from one or more in the following group：Paddy rice, wheat, millet, black rice, oat, buckwheat, myotonin and sorghum.
Fig. 4 and Fig. 5 respectively illustrate chrysophyceae using removing the rice grain and wheat berry of bran as the growing state under conditions of culture medium.Result shows, under conditions of using the rice grain and wheat berry of bran is removed as culture medium, chrysophyceae of the invention can be with fast-growth.Therefore, in one embodiment, the cereal used by the present invention is paddy rice or wheat, preferably wheat.
Term " system of culture chrysophyceae " refers to system when cultivating chrysophyceae residing for chrysophyceae.Such as system for cultivating chrysophyceae includes water, the water purification in natural surroundings, and distilled water or running water (preferably through the running water being fully exposed to the sun) are used as culture medium.The system for cultivating chrysophyceae can be the container with certain volume, such as culture tank, incubator etc..The system for cultivating chrysophyceae can also be closed waters such as pond or open waters, such as river.The volume for cultivating the system of chrysophyceae can determine according to the amount of chrysophyceae to be cultivated.
Fig. 4 and Fig. 5 also respectively illustrate growing state of the chrysophyceae under the rice grain and wheat berry that remove bran using Different adding amount.Result is pointed out, and can further promote the growth of chrysophyceae by optimizing rice grain and wheat berry addition.In one embodiment, the present invention used by grain addition be 1-50g/L, more preferably preferably 12.5-50g/L, 25-50g/L, or preferably 1-10g/L, more preferably 5-10g/L, in terms of the dry weight of grain.In one embodiment, the wheat berry of bran will be gone to be added in the aqueous system for cultivating chrysophyceae with the addition of 5-10g/L.In one embodiment, the rice grain of bran will be gone to be added in the aqueous system for cultivating chrysophyceae with the addition of 12.5-50g/L.
Fig. 6 shows growing state of the chrysophyceae under wheat berry and wheat berry+nutritive salt+vitamin culture.In one embodiment, cultural method of the invention further includes to add following nutritive salt and vitamin in the system of culture chrysophyceae：NaNO3、KH2PO4、K2HPO4, vitamin B6And vitamin B12.In one embodiment, the addition of nutritive salt and vitamin is in every 1L aqueous system：NaNO3:140mg、KH2PO4:10mg、K2HPO4:5mg, vitamin B6:1 μ g and vitamin B12:1μg。
The present invention is investigated the influence that illumination grows to chrysophyceae.Even if as shown in fig. 7, under conditions of no illumination, chrysophyceae algae strain of the invention also can under the condition of culture of wheat berry+nutritive salt+vitamin fast-growth.This represents that the growth performance of chrysophyceae algae strain of the invention is very excellent.In one embodiment, chrysophyceae of the invention is cultivated under normal lighting conditions.In one embodiment, the luminous intensity of illumination is 50-100 μm of ol photonsm-2·s-1, preferably 50 μm ol photonsm-2·s-1。
Chrysophyceae cultural method of the invention can be carried out under outdoor environment temperature.In one embodiment, chrysophyceae of the invention can be cultivated at 20-28 DEG C, preferably 22-25 DEG C, more preferably 22 ± 2 DEG C.In one embodiment, chrysophyceae of the invention is cultivated in pH is for the culture medium of 6.0-8.5, and preferably pH is 6.0-7.5.
Embodiment
The present invention will be more fully described with reference to following examples.But, there is provided following examples are only used for the explanation present invention, should not be construed as the limitation to scope and spirit of the present invention.
Experiment material：
CMBB-01 plants of chrysophyceae P.malhamensis, is separated by Inst. of Hydrobiology, Chinese Academy of Sciences and obtained, and China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in preserving number CGMCC No.11620.
Embodiment
1.
Chrysophyceae
CMBB-01
The separation and identification of strain
1.CMBB-01 plants of isolation and purification
Natural environment water body is come from comprising algae of the present invention strain algae solution, is further separated using stepwise dilution method, obtain pure algae strain, it numbered and is named as CMBB-01 plants.
2.CMBB-01 plants of Morphological Identification
Light microscope (OLYPUS, BX53,400 times) is respectively adopted and electronic scanner microscope (SEM) observes its Morphologic Characteristics.As shown in Figure 1A and 1B, at 5-10 μm, containing two flagellums for differing in size, flagellum long is long 10-15 μm, and short flagellum is long 2-3 μm, and electronic scanner microscope can be good at flagellum villous, and fine hair is long 1-1.5 μm, and short flagellum is without fine hair for CMBB-01 plants of cell dia size；CMBB-01 plants of cell includes Zhousheng chromatoplast, in yellow or golden brown.
CMBB-01 plants belongs to mixotrophism type, and photosynthesis autophyting growth can be passed through in minimal medium, and chemoheterotrophy is carried out using organic matter, and can also swallow chlorella carries out phagotrophy.
According to《CHINESE FRESHWATER algae》With《Protozoan》Etc. data Preliminary Identification, CMBB-01 plants is the species of Chrysophyta (Chrysophyta) coloured gold Cutleriales (Chromulinales) Zhui Nang algaes section (Dinobryonaceae).
3. molecular biology identification
CMBB-01 plants of genomic DNA is extracted, its 18s rDNA and rbcL gene order is expanded respectively using two kinds of different primers, the primer sequence is：
18s rDNA genes：- the AACCTGGTTGATCCTGCCAGT-3 ' of primers F 5 ' (SEQ ID NO:1)
18s rDNA genes：- the TGATCCTTCTGCAGGTTCACCTAC-3 ' of primer R 5 ' (SEQ ID NO:2)
RbcL genes：- the CAGTAGTATGGACAG-3 ' of primers F 5 ' (SEQ ID NO:3)
RbcL genes：- the CCAACTACAGTTCCAGC-3 ' of primer R 5 ' (SEQ ID NO:4)
1% agarose electrophoresis of amplified production, kit (OMEGA, USA) reclaims PCR primer, and glue reclaim product is fed directly to company's sequencing (Shanghai Sheng Gong bioengineering Co., Ltd).CMBB-01 plants of 18s rDNA gene order and rbcL gene orders are respectively such as SEQ ID NO:5 and SEQ ID NO:Shown in 6.Fig. 2 and Fig. 3 show the result that above-mentioned rbcL gene orders and 18s rDNA gene orders are compared in GenBank respectively.As shown in Figures 2 and 3, knowable to rbcL genes and 18s rDNA gene comparison results, CMBB-01 plants of evolutionary distance is closest with P.malhamensis.
Comprehensive morphological and molecular sequences information, determine CMBB-01 plants for chrysophyceae, are named as P.malhamensis CMBB-01 plants.
Embodiment
2.
Under the conditions of Different Nutrition source
CMBB-01
The measure of strain growth performance
2.1. the rice grain for removing bran is used as nutrient source
Using the rice grain of bran is removed chrysophyceae is cultivated as the nutrient source of chrysophyceae algae of the present invention strain.Meanwhile, in order to study the influence that different amounts of rice grain grows to chrysophyceae, add different amounts of rice grain respectively in identical cultivating system, the group with five rice grains of Different adding amount is set：0.25g/L, 2.5g/L, 12.5g/L, 25g/L and 50g/L.Each group sets 4 repetitions.To ensure that chrysophyceae not exclusively sinks to the bottom, daily shaking flask four times.Daily timing sampling, and counted using blood counting chamber.Each sample statistics 2 times, take four average values of repeat samples as end value.Chrysophyceae initial density is 1.78 × 104Individual/mL.Cultivation temperature is (22 ± 2) DEG C, and intensity of illumination is 50 μm of ol photonsm-2·s-1.Holding pH is between 6.0-7.5 during whole experiment.
As can be seen from Figure 4, fast-growth by chrysophyceae algae strain of the present invention is under using only the rice grain culture for removing bran.Especially, when rice grain is 12.5g/L, 25g/L and 50g/L using addition, chrysophyceae density can just reach 10 when cultivating the 2nd day6Individual/mL, 10 just can be continued to increase at the 3-4 days7Individual/mL, and then can also be slowly increased.
2.2. the wheat berry for removing bran is used as nutrient source
Using the wheat berry of bran is removed chrysophyceae is cultivated as the nutrient source of chrysophyceae algae of the present invention strain.Meanwhile, in order to study the influence that different amounts of wheat berry grows to chrysophyceae, add different amounts of wheat berry respectively in identical cultivating system, the group with six wheat berries of Different adding amount is set：1g/L, 2.5g/L, 5g/L, 10g/L, 20g/L and 40g/L.Remaining experiment condition and method are with foregoing 2.1 part.
As can be seen from Figure 5, chrysophyceae algae strain of the present invention equally being capable of fast-growth under using only the wheat berry culture for removing bran.Especially, in 2.5g/L, 5g/L and 10g/L group, chrysophyceae density can just reach 10 when cultivating the 2nd day6Individual/mL, when cultivating 3 days, 10g/L groups show highest chrysophyceae density, about 107Individual/mL, and then can also be slowly increased.
Unexpectedly, it is opposite with the expection that addition more multiple nutrient may obtain chrysophyceae density higher, experiment confirms that after culture 2 days the chrysophyceae density of (such as 20g/L and 40g/L groups) is astoundingly less than the chrysophyceae density of in initially compared with the group of the wheat berry of few additive (such as 1g/L, 2.5g/L, 5g/L and 10g/L group) in the group of the wheat berry of initial addition high.
2.3. wheat berry+nutritive salt+the vitamin for removing bran is used as nutrient source
Wheat berry+nutritive salt+vitamin medium in every liter of water by adding 140mg NaNO3、10mg KH2PO4、5mg K2HPO4, 1 μ g vitamin B6s, 1 μ g vitamin B12s, while adding 5g wheats to prepare.Chrysophyceae initial density is 2.27 × 104Individual/mL.Remaining experiment condition and method are with foregoing 2.1 part.
As can be seen from Figure 6, contrast and use the wheat berry for removing bran as nutrient source and using the wheat berry+nutritive salt+vitamin for removing bran as both condition of culture of nutrient source, result shows that the growing state of chrysophyceae strain of the present invention under the conditions of two kinds is similar to, and the addition of nutritive salt and vitamin only has certain facilitation to growing for chrysophyceae of the present invention.
2.4. CMBB-01 plants is cultivated using the method for prior art
CN 101353626B disclose a kind of chrysophyceae (i.e. Poterioochromonas sp.ZX1, preserving number is CGMCC No.2262), and the method that can cultivate chrysophyceae using water willow, three kinds of leaching liquors of plant of cattail and reed.
In order to further test the growth performance of chrysophyceae algae strain of the invention, chrysophyceae of the invention is cultivated using the method disclosed in above-mentioned document, and then following parameter is tested：The maximum growth rate of chrysophyceae, the maximum chrysophyceae density that can be reached and the time required for reaching maximum chrysophyceae density.
Experimental group 1 cultivates chrysophyceae of the invention as medium component using the wheat berry of the part of above-described embodiment 2.3, nutritive salt and vitamin.Experimental group 2 cultivates chrysophyceae of the invention using water willow, three kinds of leaching liquors of plant of cattail and reed.The initial density of the chrysophyceae of each experimental group keeps identical when culture starts.Remaining experiment condition and method are with foregoing 2.1 part.Test result is as follows：
The comparing with existing culture technique of the invention of table 1
| Project | Experimental group 1 | Experimental group 2 |
| 1.72 | 0.92 | |
| Maximum biomass (individual/mL) | ||
| Time required for reaching maximum biomass | 3-4 days | 4-5 days |
From table 1 knowable to the data of experimental group 2, even if using different cultural methods, the separated chrysophyceae algae strain of the present invention is still able to reach stand density very high in a short time that (order of magnitude reaches 106Individual/mL), this prompting chrysophyceae algae strain of the invention has excellent growth performance.And, compared to the method for above-mentioned document, the method for carrying out cultivating chrysophyceae algae strain of the invention as nutrient source using rice grain, wheat berry and wheat berry+nutritive salt+vitamin is easier, and toxigenic capacity is greatly lowered.
The above results show that the separated new chrysophyceae algae strain of the present invention has good growth performance, is capable of achieving substantial amounts of propagation in the short time.And, the technique very simple of this algae strain is cultivated, without strict condition of culture, required raw material sources are extensive and cheap.These all cause that chrysophyceae algae strain of the invention can be applied to large-scale culture application.
Embodiment
3.
Illumination pair
CMBB-01
The influence of strain growth
For the influence that research illumination grows to chrysophyceae of the present invention, (the 50 μm of ol photonsm of light group 1 are set when chrysophyceae of the present invention is cultivated-2·s-1), (the 100 μm of ol photonsm of light group 2-2·s-1) and complete darkness group (0 μm of ol photonsm-2·s-1).Every group using the wheat berry+nutritive salt+vitamin of the part of above-described embodiment 2.3 as nutrient source.Remaining experiment condition and method are with foregoing 2.1 part.
Even if as shown in fig. 7, under conditions of no illumination, chrysophyceae algae strain of the invention is used as nutrient source by using wheat berry+nutritive salt+vitamin, its density when cultivating 3 days also can reach 106Individual/mL.The growth performance of this prompting chrysophyceae algae strain of the invention is very excellent.From culture 2 days, under conditions of having light, chrysophyceae density can even more be continuously increased.Unexpectedly, for the strain of this chrysophyceae algae, experiment is found when light intensity from 50 μm of ol photonsm-2·s-1Increase to 100 μm of ol photonsm-2·s-1When, there are not significant changes in its growth efficiency.
Embodiment
4.CMBB-01
Chrysolaminaran is extracted and assay in strain
The algae solution that results are cultivated according to embodiment 2.3 is removed into impurity contained therein by filtering and impurity removing, obtain during pure CMBB-01 plants of harvest liquid is placed on freeze drier and dry 4-5 days, dried algae powder 1g is taken to be placed in 200mL beakers, plus 100mL distillation water dissolves, 90 DEG C of water-bath 1h, 5000g is centrifuged 20min, filters supernatant with multilayer gauze, and supernatant is passed through into filter paper suction filtration.4 times of ethanol of volume 95% are added in the supernatant being filtrated to get, precipitation is collected in precipitate polysaccharides, centrifugation (5000g, 20min).Sediment is dried into (75 DEG C, 24h) in an oven, the extra large fishy smell white chunks thing of acquisition is thick chrysolaminaran dry product, calculates chrysolaminaran yield.
Laminarin yield (%)=[Thick many candies dry product quality (g)/algae silty amount (g)] × 100%
By calculating, laminarin content is up to 24.8% in CMBB-01 plants, hence it is evident that suitable with laminarin content in true eyespot algae higher than laminarin content in other chrysophyceae or sea-tangle.
Laminarin comparision contents in CMBB-01 plants of table 2 and other algae
| Species | Laminarin yield | Referring to |
| CMBB-01 plants | 24.8% | |
| Isochrysis galbana | 7%-22.1% | Pu third of the twelve Earthly Branches virtue etc., food science and technology, 2012 (7):186-190. |
| True eyespot algae | 13.01%-24.88% | Han Juan, Ji'nan University, 2013. |
| Sea-tangle | 1%-18.31% | Kang Yanyan etc., food research and development, 2006,27 (1):59-62. |
Although having been used for purpose of explanation discloses the preferred embodiment of the present invention, those skilled in the art it is understood that can make various changes, increase and replace, without deviating from the scope and spirit of such as appended claim present invention disclosed.
Claims (8)
1. CMBB-01 plants of chrysophyceae Poterioochromonas malhamensis, it is with preserving number
CGMCC No.11620 are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
The heart.
2. a kind of cultural method of the chrysophyceae of claim 1, including cereal is removed into bran grain as gold
During the nutrient source of algae growth adds the aqueous system for cultivating chrysophyceae, wherein the cereal be selected from
One or more in the following group：Paddy rice, wheat, millet, black rice, oat, buckwheat, myotonin and
Sorghum, preferably paddy rice and wheat.
3. cultural method as claimed in claim 2, further includes to add in the system of culture chrysophyceae
Enter following nutritive salt and vitamin：NaNO3、KH2PO4、K2HPO4, vitamin B6And vitamin
B12。
4. cultural method as claimed in claim 2 or claim 3, wherein added in the aqueous system
The amount of grain is 1-50g/L, more preferably 5-15g/L, in terms of the dry weight of grain.
5. the cultural method as any one of claim 2-4, wherein when cereal is paddy rice,
The amount added is 12.5-50g/L.
6. the cultural method as any one of claim 2-5, wherein when cereal is wheat,
The amount added is 5-10g/L.
7. the cultural method as any one of claim 2-6, wherein in per 1L aqueous system
The addition of nutritive salt and vitamin is：NaNO3:140mg、KH2PO4:10mg、K2HPO4:5mg、
Vitamin B6:1 μ g and vitamin B12:1μg。
8. the cultural method as any one of claim 2-7, wherein the culture 20-28 DEG C,
It is preferred that 22-25 DEG C of temperature, 50-100 μm of ol photonsm-2·s-1, preferably 50 μm ol
photons·m-2·s-1Luminous intensity, and carried out under the pH of 6.0-8.5.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810902A (en) * | 2019-01-03 | 2019-05-28 | 中国科学院水生生物研究所 | A kind of fast culture process of chrysophyceae |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109810902A (en) * | 2019-01-03 | 2019-05-28 | 中国科学院水生生物研究所 | A kind of fast culture process of chrysophyceae |
| CN114774284A (en) * | 2022-05-24 | 2022-07-22 | 安徽师范大学 | Method for controlling microcystis aeruginosa bloom by combining phaeoflagellate malanema and caprylic acid |
| CN114774284B (en) * | 2022-05-24 | 2024-03-26 | 安徽师范大学 | Method for controlling microcystis aeruginosa water bloom by combining Ma Lem calicivilian and n-octanoic acid |
| CN115322906A (en) * | 2022-08-18 | 2022-11-11 | 吉林省水产科学研究院(吉林省渔业生态环境及水产品质量监督检测中心) | Culture solution for culturing chrysophyceae monarda and culture method thereof |
| CN115322906B (en) * | 2022-08-18 | 2024-03-12 | 吉林省水产科学研究院(吉林省渔业生态环境及水产品质量监督检测中心) | Culture solution for culturing dinoflagellate and culture method thereof |
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