CN2494360Y - Internal circulating membrane filtration colony culture device - Google Patents
Internal circulating membrane filtration colony culture device Download PDFInfo
- Publication number
- CN2494360Y CN2494360Y CN 01248557 CN01248557U CN2494360Y CN 2494360 Y CN2494360 Y CN 2494360Y CN 01248557 CN01248557 CN 01248557 CN 01248557 U CN01248557 U CN 01248557U CN 2494360 Y CN2494360 Y CN 2494360Y
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- cup
- filtration
- container
- utility
- membrane filtration
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Abstract
The utility model discloses a bacteria collecting culturing device of internal cycle filtration. The utility model comprises a transparent cup body, a filtration disk, a liquid storage tank and a clamp. One side of the cup body is provided with calibration line. Circular right angle flanging is at cup mouth. The bottom of the cup is provided with two cylindrical interface blocked by adhesive plugging. The filtration disc is outlet with flange, a seal ring, a microporous membrane and a screen is installed therein. The clamp is an open clamp clamped at the interface of the cup body and the liquid storage tank. The bottom of the liquid storage tank is provided with an outlet. The utility model can realize the aim of totally closing, cycle washing internal wall of infusion sets for single use, membrane filtration, collecting bacteria totally, injecting medium directly and culturing directly, and has the advantages of repeating use, saving investment and low cost.
Description
The utility model relates to a kind of improvement of colony culture device, is a kind of internal recycle membrane filtration colony culture device, belongs to technical field of medical instruments.
Existing full envelope steriling test device, seen the report that Chinese patent Z.L962458120.0 " uses colony culture device " repeatedly, be that two cylinder-shaped joints are arranged above transparent cup, stainless steel base end opening does not seal, there is not cylinder-shaped joint yet, can not realize that " using colony culture device repeatedly " and " disposable infusion set " form closed cycle system, can't realize that also sterile water for injection circulates flushing " disposable infusion set " inwall continuously and collects bacterium process completely in system.
The utility model discloses a kind of internal recycle membrane filtration colony culture device, and the collection bacterium that is fit to " disposable infusion set " steriling test is cultivated.Make " disposable usability transfusion device " steriling test realize totally-enclosed, internal recycle, membrane filtration, collection bacterium fully, use, save one-time investment, economical and practical repeatedly.
Solution of the present utility model is to realize like this, form by transparent cup, filtering table, container for storing liquid, clip, wherein, one side of transparent cup has scale marks, the bottom of cup is provided with two column interfaces, and order is equipped with sealing-ring, millipore filtration and sieve in the filtering table, the two ends of filtering table be flange arrangement respectively with cup and container for storing liquid sealed attachment, clip is stuck in the interface of cup and container for storing liquid for the open type clip, and the bottom of container for storing liquid is provided with outlet.
Utilize the utility model when making " disposable infusion set " steriling test, in container for storing liquid, inject the water for injection of certain volume earlier, with clip the flange on filtering table and the container for storing liquid is tightened, put into sealing-ring, sieve, millipore filtration and sealing-ring in the filtering table successively then, cover transparent cup at last, tighten with clip.With said apparatus and silicone tube and with the common moist heat sterilization of the substratum of transfusion bottle.During connection, " disposable infusion set " outer cone joint one end is connected on the cylindrical outlet on the container for storing liquid, bottle plug puncture unit one end is connected on the silicone tube, and the silicone tube the other end is connected on the cylinder-shaped joint on the transparent cup, and the silicone tube interlude is fixed on the peristaltic pump.Open peristaltic pump like this and can under full closeding state, realize water for injection flushing " disposable infusion set " inner surface wall, membrane filtration, the working cycle of collection bacterium.When reaching regulation flush volume amount, close peristaltic pump, pull up the bottle plug puncture unit of " disposable infusion set ", silicone tube is connected on the bottle plug puncture unit that has air filter (can remove the above particulate of 0.3um), and be inserted on the infusion bottle that substratum is housed, it is stifled to pull up on the transparent cup 1 glue on another cylinder-shaped joint.Open peristaltic pump, make substratum enter into transparent cup and reach the prescribed volume amount; Stop the peristaltic pump running, pull up the silicone tube on the transparent cup,, open the flange on filtering table and the container for storing liquid with stifled last two cylinder-shaped joints of glue, the transparent cup of internal recycle membrane filtration colony culture device upwards is positioned in the incubator, and temperature and time is cultivated, is observed according to the rules.Positive control directly adds contrast bacterium liquid in internal recycle membrane filtration colony culture device; Negative control directly connects with silicone tube.
Positively effect of the present utility model is: can form closed cycle system with " disposable infusion set ", realize that sterile water for injection circulates flushing " disposable infusion set " inwall continuously, filters the continuous working cycle that collects bacterium in system.When making steriling test with internal recycle membrane filtration colony culture device simultaneously, the peristaltic pump that uses in the time of can sharedly doing readily oxidizable substance needn't be bought germ collector in addition, saves facility investment.For " disposable infusion set " steriling test, can reach totally-enclosed, internal recycle, membrane filtration, collection bacterium fully, use repeatedly, reduce investment outlay, advantage such as economical and practical.
Fig. 1 is the utility model structure principle chart.
The transparent cup of 1-, 2-filtering table, 3-container for storing liquid, 4-clip,
5-sealing-ring, 6-millipore filtration, 7-sieve, 8-outlet, 9-interface.
Further specify the utility model below in conjunction with accompanying drawing: transparent cup 1 usefulness synthetic glass is made, and filtering table 2, container for storing liquid 3 usefulness stainless steels are made, and sealing-ring 5 usefulness silicon rubber are made.Transparent cup 1, filtering table 2, container for storing liquid 3, clip 4 are formed internal recycle membrane filtration colony culture device.Transparent cup 1 is provided with scale marks, and rim of a cup has all right angles flange, and two cylindrical interfaces of being blocked by glue 9 are arranged at the bottom of cup 1.Sealing-ring 5, millipore filtration 6 and sieve 7 are housed in the filtering table 2.The two ends of filtering table 2 be flange arrangement respectively with cup 1 and container for storing liquid 3 sealed attachment, clip 4 is stuck in cup 1 and container for storing liquid 3 interfaces for the open type clip, the bottom of container for storing liquid 3 be provided with the outlet 8.
Claims (1)
1, a kind of internal recycle membrane filtration colony culture device, it is characterized in that: by transparent cup (1), filtering table (2), container for storing liquid (3), clip (4) is formed, wherein, one side of transparent cup (1) has scale marks, the bottom of cup (1) is provided with two column interfaces (9), order is equipped with sealing-ring (5) in the filtering table (2), millipore filtration (6) and sieve (7), the two ends of filtering table (2) be flange arrangement respectively with cup (1) and container for storing liquid (3) sealed attachment, clip (4) is stuck in the interface of cup (1) and container for storing liquid (3) for the open type clip, and the bottom of container for storing liquid (3) is provided with outlet (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01248557 CN2494360Y (en) | 2001-07-09 | 2001-07-09 | Internal circulating membrane filtration colony culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01248557 CN2494360Y (en) | 2001-07-09 | 2001-07-09 | Internal circulating membrane filtration colony culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2494360Y true CN2494360Y (en) | 2002-06-05 |
Family
ID=33659176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01248557 Expired - Fee Related CN2494360Y (en) | 2001-07-09 | 2001-07-09 | Internal circulating membrane filtration colony culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2494360Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881903A (en) * | 2014-03-21 | 2014-06-25 | 武汉介观生物科技有限责任公司 | Rare cell filter and application thereof |
-
2001
- 2001-07-09 CN CN 01248557 patent/CN2494360Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881903A (en) * | 2014-03-21 | 2014-06-25 | 武汉介观生物科技有限责任公司 | Rare cell filter and application thereof |
| CN103881903B (en) * | 2014-03-21 | 2016-05-04 | 武汉介观生物科技有限责任公司 | rare cell filter and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhang Jingping Document name: Notice of termination |
|
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |