CN2395277Y - Combined quick diagnostic reaction board for antigen echinococcosis - Google Patents
Combined quick diagnostic reaction board for antigen echinococcosis Download PDFInfo
- Publication number
- CN2395277Y CN2395277Y CN 99219078 CN99219078U CN2395277Y CN 2395277 Y CN2395277 Y CN 2395277Y CN 99219078 CN99219078 CN 99219078 CN 99219078 U CN99219078 U CN 99219078U CN 2395277 Y CN2395277 Y CN 2395277Y
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- CN
- China
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- antigen
- echinococcosis
- fiber membrane
- utility
- model
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- Expired - Lifetime
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a combined quick diagnostic reaction board for antigen echinococcosis. The utility model comprises an upper cap and a socket, and a checking hole is arranged on the upper cap. Fiber membrane is arranged between the checking hole of the upper cap and the socket, and four kinds of antigen are coated on the fiber membrane. The first kind of antigen part purified fine grain hydatid liquid antigen, the second kind of antigen is part purified fine particle echinococcus scolex antigen, the third kind of antigen is purified hydatid fluid antigen, and the fourth kind of antigen is purified alveolar hydatid antigen. The combined quick diagnostic reaction board for antigen echinococcosis has the advantages of simple structure and easy operation. The utility model is used for the early diagnosis of echinococcosis and differential diagnosis, the result specificity is strong, the sensitivity is high, and the rate of missed diagnosis is reduced. Result can be worked out in 3 minutes, and the utility model is especially suitable for screening samples.
Description
The utility model relates to the reaction plate that is used to diagnose echinococcosis, is a kind of combined antigen echinococcosis quick diagnosis reaction plate.
Echinococcosis is a kind of common parasitic disease that is popular in the cattle-producing area, belongs to one of zoonosis of serious harm health.All there is report western part of China, the north, middle part and 11 provinces and regions of northeast region-by-region, wherein rank first in the whole country with Xinjiang echinococcosis gradient of infection.At present, the main method of conventional echinococcosis laboratory inspection is Ka Songni test, counter immunoelectrophoresis and indirect hemagglutination test etc., and its false positive and false negative rate are all higher, and complicated operation, minute are longer.
In view of the problem of above-mentioned existence, the purpose of this utility model is to design a kind of simple in structure, easy to operate combined antigen echinococcosis quick diagnosis reaction plate.
The purpose of this utility model realizes by following measure: this combined antigen echinococcosis quick diagnosis reaction plate comprises loam cake and base, in the last detection hole that covered, between the detection hole of loam cake and base, fiber membrane is arranged, on fiber membrane, be coated with four kinds of different antigens, first kind of antigen is partial purification Wall of Echinococcus liquid antigen, second kind of antigen is partial purification particulate echinococcus scolex antigen, the third antigen is purified cyst fluid antigen, and the 4th kind of antigen is the alveolar hydatid antigen of purifying.Between fiber membrane and the base thieving paper is arranged.Be coated with positive reference mark on the fiber membrane, and positive reference mark is a human serum.
Accompanying drawing 1 is the main TV structure synoptic diagram of the utility model most preferred embodiment,
Accompanying drawing 2 be the a-a of accompanying drawing 1 to the sectional structure synoptic diagram,
Coding in the accompanying drawing is respectively: 1 is loam cake, and 2 is base, and 3 for detecting the hole, and 4 is fiber membrane, and 5 is thieving paper, and 6 are documentary film; A is first kind of antigen, and B is second kind of antigen, and C is the third antigen, and D is the 4th a kind of antigen, the positive reference mark of E.
Below in conjunction with most preferred embodiment and accompanying drawing the utility model is further described:
Shown in attached Fig. 1 and 2, this combined antigen echinococcosis quick diagnosis reaction plate comprises loam cake 1 and base 2, the hole 3 of detection is arranged on loam cake 1, between the detection hole 3 of loam cake 1 and base 2, fiber membrane 4 is arranged, bag is by four kinds of different antigens on fiber membrane 42, and first kind of antigen A is partial purification Wall of Echinococcus liquid antigen, and second kind of antigen B is partial purification particulate echinococcus scolex antigen, the third antigens c is a purified cyst fluid antigen, and the 4th kind of antigen D is the alveolar hydatid antigen of purifying.
During use: at first, the utility model is kept flat, get 20 μ l serum or 40 μ l whole bloods are put in the centrifuge tube, and splash into 5 of A liquid (whole blood splashed into after a minute), mixing (whole blood needed 2000rpm centrifugal 2 minutes if produce muddiness), get 3 of above-mentioned mixed liquor supernatants (or drawing 100 μ l), splash in the detection of the present utility model hole 3; Secondly, drip 3 B liquid in detecting hole 3, drip 3 C liquid in detecting hole 3, drip 3 B liquid in detecting hole 3; Observe and write down the result then, can make the result in general about 3 minutes, positive: "+" pale red, " ++ " redness, " +++" aubergine, negative: "-" A, B, C, if D place redfree spot or the feminine gender that is judged to of vestige only occurs is A, B, C, the D place has one or more more weak spot to occur, needs check after two weeks, wherein, A liquid is the sample dilution, and interior is Tris-HCl damping fluid+5%Tween20, and B liquid is cleansing solution, interior is Tris-HCl damping fluid+4%Tween20+9%NaCl, C liquid is the anti-human IgG antibody of gold mark, includes collaurum, anti-human IgG and gelatin, multitudinous sugar.
For the ease of absorbing dropping liquid, as shown in Figure 2, between fiber membrane 4 and the base 2 thieving paper 5 is arranged.
As shown in Figure 1, on fiber membrane 4, be coated with positive reference mark E, and positive reference mark E is a human serum,, illustrates that then this combined antigen echinococcosis quick diagnosis reaction plate lost efficacy and can not use if E place, positive reference mark do not develop the color.Wherein, above-mentioned fiber membrane 4 can be acetate film.
The utility model is simple in structure, easy and simple to handle, is applied to the early diagnosis and the antidiastole of echinococcosis, makes high specificity as a result, and is highly sensitive, significantly reduced rate of missed diagnosis, generally only need promptly look in 3 minutes and make the result, so be specially adapted to the examination of sample.
Claims (3)
1. combined antigen echinococcosis quick diagnosis reaction plate, it is characterized in that comprising loam cake and base, in the last detection hole that covered, between the detection hole of loam cake and base, fiber membrane is arranged, be coated with four kinds of different antigens on fiber membrane, first kind of antigen is partial purification Wall of Echinococcus liquid antigen, and second kind of antigen is partial purification particulate echinococcus scolex antigen, the third antigen is purified cyst fluid antigen, and the 4th kind of antigen is the alveolar hydatid antigen of purifying.
2. combined antigen echinococcosis quick diagnosis reaction plate according to claim 1 is characterized in that between fiber membrane and the base thieving paper being arranged.
3. combined antigen echinococcosis quick diagnosis reaction plate according to claim 1 it is characterized in that being coated with positive reference mark on the fiber membrane, and positive reference mark is a human serum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99219078 CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combined quick diagnostic reaction board for antigen echinococcosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99219078 CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combined quick diagnostic reaction board for antigen echinococcosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2395277Y true CN2395277Y (en) | 2000-09-06 |
Family
ID=34010100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99219078 Expired - Lifetime CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combined quick diagnostic reaction board for antigen echinococcosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2395277Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN102608321A (en) * | 2012-02-29 | 2012-07-25 | 厦门大学 | Echinococcus multilocularis circulating antigen dot immunogold filtration kit and preparation method |
| CN106092988A (en) * | 2016-06-08 | 2016-11-09 | 新疆医科大学第附属医院 | The method for quick of Echinococcus hydatid cyst protein molecular based on SOI self-luminous characteristic |
| CN107402298A (en) * | 2016-05-18 | 2017-11-28 | 上海新吉而生物科技有限公司 | A kind of dog excrement echinococcosis antigen quick detection kit and its application method |
| CN110261615A (en) * | 2019-07-04 | 2019-09-20 | 中国动物疫病预防控制中心(农业农村部屠宰技术中心) | Cystic echinococcosis diagnostic kit and its application |
-
1999
- 1999-08-09 CN CN 99219078 patent/CN2395277Y/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608321A (en) * | 2012-02-29 | 2012-07-25 | 厦门大学 | Echinococcus multilocularis circulating antigen dot immunogold filtration kit and preparation method |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN107402298A (en) * | 2016-05-18 | 2017-11-28 | 上海新吉而生物科技有限公司 | A kind of dog excrement echinococcosis antigen quick detection kit and its application method |
| CN106092988A (en) * | 2016-06-08 | 2016-11-09 | 新疆医科大学第附属医院 | The method for quick of Echinococcus hydatid cyst protein molecular based on SOI self-luminous characteristic |
| CN106092988B (en) * | 2016-06-08 | 2019-03-01 | 新疆医科大学第一附属医院 | The rapid detection method of Echinococcus hydatid cyst protein molecular based on the spontaneous light characteristic of SOI |
| CN110261615A (en) * | 2019-07-04 | 2019-09-20 | 中国动物疫病预防控制中心(农业农村部屠宰技术中心) | Cystic echinococcosis diagnostic kit and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |