CN2356041Y

CN2356041Y - Large scale continuous cultivating device for microorganism and animal and vegetable cell

Info

Publication number: CN2356041Y
Application number: CN 98251832
Authority: CN
Inventors: 王朝华; 梁婉琪; 张大兵; 蒋国发; 钟罡
Original assignee: Individual
Current assignee: Individual
Priority date: 1998-12-26
Filing date: 1998-12-26
Publication date: 1999-12-29
Anticipated expiration: 2008-12-26

Abstract

The utility model relates to a large scale continuous cultivating device for microorganism and animal and vegetable cell, which is characterized in that carrier networks positioned on the upper parts of a feed pipe and an aerator pipe of the inner shell are composed of at least one unit carrier network, however, biological carriers uniformly distributed in the unit carrier network are composed of fixed layers and adhesion layers which are arranged on the fixed layers and present fluffy closely distributed interleaving structures. The utility model has stable running, long service life, and has loose operating condition. Due to the fact that the adhesion objects on the biological carrier are not easy to fall and run off, the adhesion amount is relatively great, and the nutrient component in the culture medium can be sufficiently utilized, the cost is reduced, and the waste is reduced.

Description

A kind of microorganism and animal and plant cell large scale bactogen of can be used for

The utility model relates to a kind of microorganism and cell culture apparatus, particularly a kind of microorganism and animal and plant cell large scale bactogen of can be used for.

At present, the device that is used for the extensive cultured continuously of microorganism, a kind of high-performance bio fermentation unit was once arranged, it is mainly by body skin, inner housing, top cover constitutes, it is to adopt a framework that is provided with on the bracing frame in how much unit carrier strings that connected and composed by spherical how much unit carriers of ordered arrangement and the inner housing to connect and compose the cubic network carrier, by cheap rock wool as solid support material, make engineering bacteria utilize the nutritive ingredient in the substratum to breed in a large number by stirring or aeration, and attached on the geometry unit carrier, when flowing from bottom to top, the substratum that is added continuously by lower housing portion rises in the process, the nutritive ingredient that engineering bacteria will make full use of in the substratum is produced a large amount of purpose products, realizes the purpose of the extensive cultured continuously of microorganism.But because how much unit carriers of this device are solid sphere, so effective bond area of microorganism is less, and the nutritive ingredient in the substratum, gas are difficult to enter into spheroid inside, make that efficient is low, fermentation time is long, fermentation costs is higher.And be bound in how much unit carrier strings on the framework when being subjected to water for a long time, when gas impacts, also easily causing the carrier string to come off by rope, whole carrier structure is destroyed.The porose microcarrier that sintering such as glass, pottery form is arranged and be placed in the device bead carrier that vegetable cell and embedding medium are mixed and made into and the carrier that is used for zooblast or vegetable cell bactogen adopts respectively, they are from also having above-mentioned deficiency in varying degrees, particularly in the vegetable cell bactogen, embedding medium in the bead carrier is because of costing an arm and a leg, cause production cost higher, and make its such device be difficult to apply.

The purpose of this utility model is to provide a kind of microorganism and animal and plant cell large scale bactogen of can be used for, and its production cost is low, efficient is high, long service life.

Concrete solution is: the carrier network that is positioned at inner housing feed-pipe and aeration tube top is made of at least one unit carrier network, and on the every layer of dividing plate that is arranged on inner housing that the unit carrier network is orderly, and dividing plate is placed on the brace table of inner housing correspondence.The unit carrier network mainly by framework with and interior uniform bio-carrier constitute.Bio-carrier is made up of immovable bed and the adhesion layer that is arranged on the immovable bed.

In addition then, the adhesion layer of described bio-carrier is bulk densely covered pilotaxitic texture, and its material is a fiber yarn, and this fiber yarn adopts organic and inorganic or organic and inorganic blended fiber yarn all can.When adopting the organic fibre silk, its best materials is a nylon.

When being used for the extensive cultured continuously of microorganism, the engineering bacteria of its adding can make full use of nutritive ingredient and a large amount of propagation in the microbiological culture media under the working order under the steady temperature environment, and on the adhesion layer attached to bio-carrier, when the substratum that is added continuously by lower housing portion is flowing in the process that rises from bottom to top, engineering bacteria will make full use of the nutritive ingredient in the microbiological culture media and produce a large amount of purpose products, realize the purpose of large scale continuous prod.

When being used for the extensive cultured continuously of zooblast or vegetable cell, under the working order, zooblast that adds or vegetable cell can make full use of the nutritive ingredient in animal substratum or the plant culture under steady temperature, a large amount of propagation, and attached on the adhesion layer, in the process that the bottom-up stream of its substratum rises, zooblast or vegetable cell will make full use of the nutritive ingredient in its substratum, produce a large amount of purpose products, realize the purpose of large scale continuous prod.

The utility model constitutes an overall network by the unit carrier network, and adopt have bulk densely covered adhesion layer, quality is light, the bio-carrier that is difficult for loss, make that not only the unit carrier network is easy to change, and greatly increased effective bond area, and has a good permeability, this device is stable, long service life, efficient height, has loose operational conditions, because dirt settling difficult drop-off, loss, adhesion amount on the bio-carrier are bigger, and can utilize nutritive ingredient in the substratum fully, thereby greatly reduce cost, reduced waste.

In conjunction with the accompanying drawings the utility model is described in detail below by embodiment:

Fig. 1 is the structural representation of the utility model embodiment 1.

Fig. 2 is the D portion enlarged view of Fig. 1.

Fig. 3 is the structural representation of the utility model embodiment 2.

Fig. 4 is the unit carrier network structural representation of the utility model embodiment 3.

Embodiment 1:

Fig. 1, among Fig. 2, housing is made up of body skin (4) and inner housing (5), and body skin (4), inner housing (5) and in, the ring-type capping (11) of top welding connects and composes a thermostatical circulating water cavity (20) between body skin, the bottom of body skin (4) and top are respectively arranged with a thermostatical circulating water input tube (3) and output tube (16), top cover (14) is arranged on the top of inner housing (5), and by the sealing that interfixes of bolt (12) and sealing-ring (15), feed-pipe (6), aeration tube (7) all is arranged on the bottom of inner housing (5), the bottom of inner housing (5) is provided with a sediment delivery pipe (1), product output tube (17) is arranged on the top of inner housing (5), and escape pipe (13) is positioned on the top cover (14).On the inner housing (5) on feed-pipe (6) and aeration tube (7) top, be welded with brace table (19), be used for supporting clapboard (18), the unit carrier network is placed on dividing plate.

In the unit carrier network, bio-carrier (8) adopts the bar shaped bio-carrier, and each bar shaped bio-carrier is distributed in the framework (9) it by being arranged on the locating shaft (10) on the framework.In the bio-carrier, what two adhesion layers (21), (23) adopted is the nylon fiber wire material, and is the bulk densely covered shape structure that interweaves and is woven on positive and negative two surfaces of immovable bed (22).

During work, at first feed steam and carry out autoclaving, open thermostatical circulating water after cooling, device is maintained in the suitable temperature range, when being used for the extensive cultured continuously of microorganism, add fermentation engineering bacterium and microbiological culture media by feed-pipe (6), after substratum floods unit carrier network on the dividing plate (18), pump into the filtration sterile air by aeration tube (7), engineering bacteria begins a large amount of propagation under adapt circumstance, and the engineering bacteria of propagation is on the bulk densely covered adhesion layer attached to bio-carrier, engineering bacteria utilizes the nutritive ingredient in the microbiological culture media will produce a large amount of purpose products in the process that the bottom-up stream of substratum rises, and by the output of product output tube.

When being used for animal or plant cell large scale cultured continuously, can be replaced with zooblast or vegetable cell by the engineering bacteria that feed-pipe adds, microbiological culture media replaces with the animal substratum or plant culture gets final product.

When the device long-term operation, when its underpart settling is too much, but Open valve (2) is with its discharge.When need are changed the unit carrier network, can open top cover (14), extract from top easily.

Embodiment 2:

Among Fig. 3, remove inner housing (5) and be provided with 4 layers of dividing plate (18), and, orderly being placed with outside 36 unit carrier network (24) on every layer of dividing plate, all the other structures are identical with embodiment 1.

Embodiment 3:

In the present embodiment, removing the unit carrier network is to be made of fan-shaped framework (25) and interior uniform bio-carrier (8) thereof, and constitutes a cylinder shape carrier network by 4 unit carrier network and be placed on the dividing plate of inner housing.All the other structures are identical with embodiment 1.

Claims

1, a kind ofly can be used for microorganism and moving, the extensive bactogen of vegetable cell, by body skin (4), inner housing (5) and in, the ring-type capping (11) of top welding connects and composes a thermostatical circulating water cavity (20) between body skin, the bottom of body skin (4) and top are respectively arranged with a thermostatical circulating water input tube (3) and output tube (16), top cover (14) is arranged on the top of inner housing (5), feed-pipe (6), aeration tube (7) all is arranged on the bottom of inner housing (5), the bottom of inner housing (5) is provided with a sediment delivery pipe (1), product output tube (17) is arranged on the top of inner housing (5), air outlet (13) is positioned on the top cover (14), it is characterized in that the carrier network that is positioned at inner housing feed-pipe (6) and aeration tube (7) top is made of at least one unit carrier network, on the every layer of dividing plate (18) that is arranged on inner housing that the unit carrier network is orderly, and dividing plate is placed on the brace table (19) of inner housing correspondence, the unit carrier network mainly by framework with and interior uniform bio-carrier (8) constitute, and bio-carrier is by immovable bed (22) and is arranged on adhesion layer (21) on the immovable bed, (23) form.

2, a kind of microorganism and animal and plant cell large scale bactogen of can be used for according to claim 1 is characterized in that the adhesion layer (21) of bio-carrier, (23) are bulk densely covered pilotaxitic texture, and its material is a fiber yarn.