CN2218181Y

CN2218181Y - Gene increasing instrument

Info

Publication number: CN2218181Y
Application number: CN 94242820
Authority: CN
Inventors: 顾谷频; 张友成; 倪莉; 陈文炎
Original assignee: JOINT-VENTURE MEIDA BIOLOGIC INSTRUMENT Co Ltd ZHENJIANG CITY
Current assignee: JOINT-VENTURE MEIDA BIOLOGIC INSTRUMENT Co Ltd ZHENJIANG CITY
Priority date: 1994-11-05
Filing date: 1994-11-05
Publication date: 1996-01-24
Anticipated expiration: 2004-11-05

Abstract

The utility model relates to an instrument used for nucleic acid amplification in the technical field of biologic, medicine, etc. A mechanical part uses a multi-cavity single row structure, and a test tube stand is arranged on a test tube holder which can be horizontally moved. The utility model has the advantages of long service life, less power consumption, high operational reliability, high heat stability, high homogeneity, and lower cost.

Description

Gene-amplificative instrament

The utility model relates to a kind of instrument that technical fields such as biology, medical science increase to nucleic acid that is applied to.

Gene-amplificative instrament claims the instrument that automatically increases again, and for realizing that the automatization round pcr designs, purpose is in the dna replication dna process nucleic acid to be increased specially; And round pcr is a special kind of skill of growing up of the nineties in the world, now be used for multiple research work such as domestic animal, poultry, aquatic products, variety of crops improvement, paternity test, early stage AIDS diagnosis, had practical value especially for legal medical expert, public security, the administration of justice, quarantine, environmental sanitation, medical department.The external at present gene-amplificative instrament that occurs, adopted single working chamber on the structure, and adopt electrically heated or semi-conductor heating, wind refrigeration or modes such as semiconductor refrigerating or freon refrigeration to realize that the temperature cycle between 4 ℃-99 ℃ changes, there is following weak point in above-mentioned prior art: (1) adopts a working chamber job, device heats, cooling, halted state change frequent, shorten device lifetime greatly, power consumption is big, and temperature variation is owing to exist certain inertia, easily produce overshoot in the temperature cycle process, working reliability and quality are difficult for guaranteeing like this; (2) adopt the transmission medium of air in the working chamber, thereby thermostability in the circulation, homogeneity are lower as thermal cycling; (3) adopt a working chamber, temperature rate-controlling difficulty is big, the control section complexity of device, thereby cost height.

The purpose of this utility model is to overcome above-mentioned weak point of the prior art, provide a kind of life-span long, power consumption is little, working reliability is high, thermostability, homogeneity high and lower-cost gene-amplificative instrament.

The purpose of this utility model is achieved in that a kind of gene-amplificative instrament, form by a circuit control section and a mechanical part, said mechanical part comprises frame, working chamber, test-tube stand, in its structure, said working chamber number is at least two, their single one-tenth delegation, fill liquid in the working chamber, said test-tube stand movably connects with a test tube flat, and said test-tube stand and is installed in the transmission mechanism that the driving test-tube stand on the said test tube flat moves up and down and links, said test tube flat be installed on the horizontal guide rail of frame and with one drive its drive unit that moves horizontally and link.

Below in conjunction with embodiment (accompanying drawing), the utility model is further described.

Fig. 1 is the utility model outside view;

Fig. 2 is the utility model embodiment one mechanical part front view;

Fig. 3 is the A-A sectional view among Fig. 2;

Fig. 4 is that B among Fig. 3 is to view;

Fig. 5 is the utility model embodiment one mechanical part vertical view;

Fig. 6 is the utility model embodiment three mechanical part master pseudosections;

Fig. 7 is the C-C sectional view among Fig. 6.

The utility model gene-amplificative instrament is by a circuit control section and a machinery Part forms, and mechanical part comprises frame 1, working chamber 2, rack for test tube 3, In its structure, the working chamber number is at least two, their single one-tenth delegation, Fill liquid in the working chamber, rack for test tube movably connects with a test tube flat 4, and And rack for test tube and is installed in, and driving rack for test tube on the test tube flat moves up and down Transmission device links, and test tube flat is installed on the horizontal guide rail 5 of frame And with one drive its drive unit that moves horizontally and link.

Embodiment one

In the present embodiment, the number of working chamber is 4, and the liquid in the working chamber is water; Test-tube stand links with said test tube flat by a longitudinal rail 6, and transmission mechanism 7 is a slider-crank mechanism or an eccentric mechanism, and it is linked by a motor that is installed on the test tube flat; Drive unit comprises a chain gearing and a position control device, in the said chain gearing, one chain 8 is wrapped in two bogie wheels 9 that are in same horizontal plane, on 10 and one driving wheel 11, chain between test tube flat and two bogie wheels links, position control device comprises a guide groove that parallels with horizontal guide rail 12, the upper and lower plates of guide groove has corresponding through hole 13 on corresponding to the working chamber position, the upper and lower opening of each through hole is provided with a photodiode and a photosensitive receiver pope respectively, two pipes link with said circuit control section, and a plate washer 14 that is installed on the test tube flat inserts in the said guide groove.The circuit control section adopts a PC computer controlling machine or a monolithic computer controlling machine, and the former CRT shows, the Chinese and western language man-machine dialogue, and the large vol canned data, printer interface, the PC computer can use separately, and the latter is easy and simple to handle, and instrument cost is low, and charactron shows.The embodiment operating process is as follows: set four temperature in the working chamber respectively by the temperature control system in the computer, generally temperature is set at 25 ℃-65 ℃, 40 ℃-80 ℃, 60 ℃-99 ℃, 4 ℃-8 ℃ successively, the running of the position control system control motor in the computer, the signal of the upper-lower position of test-tube stand is transmitted by two vernier switches that are installed on the test tube flat, the signal of the position, the left and right sides of test-tube stand is then by above-mentioned phototube transmission, by to the computer setting program, test-tube stand just can move by the characteristics of motion of expection, thereby satisfies job requirement.

Realize example two

Identical with embodiment one overall structure, unique different be that wherein chain gearing has changed wire rope gearing into, promptly a wireline is wrapped on two bogie wheels and a driving wheel that are in same horizontal plane, and the wireline between test tube flat and two bogie wheels links.

Embodiment three

Drive unit in the present embodiment comprises a rack and pinion drive, tooth bar 15 is fixed on the frame, the gear 16 that is meshed with tooth bar is housed on the test tube flat, gear links with the motor that is installed on the test tube flat, machine operation, by the engagement of rack-and-pinion, test tube flat can be realized moving horizontally.

Compared with prior art, the utlity model has following advantage:

1, adopt the work of multi-cavity system, in the course of work, each working chamber In a single day middle temperature is set and be need not to change, so the life-span of device is long, low in energy consumption, The functional reliability height;

2, adopt liquid for passing warm medium, thereby thermally-stabilised in the thermal cycle Property, uniformity height;

3, the control difficulty is little, thereby control section is simpler, and cost is lower.

Claims

1, a kind of gene-amplificative instrament, form by a circuit control section and a mechanical part, said mechanical part comprises frame, working chamber, test-tube stand, it is characterized in that: said working chamber number is at least two, their single one-tenth delegation, fill liquid in the working chamber, said test-tube stand movably connects with a test tube flat, and said test-tube stand and is installed in the transmission mechanism that the driving test-tube stand on the said test tube flat moves up and down and links, said test tube flat be installed on the horizontal guide rail of frame and with one drive its drive unit that moves horizontally and link.

2, gene-amplificative instrament according to claim 1 is characterized in that: the number of said working chamber is 4, and the liquid in the working chamber is water.

3, gene-amplificative instrament according to claim 1 is characterized in that: said test-tube stand links by a longitudinal rail and said test tube flat, and said transmission mechanism is a slider-crank mechanism or an eccentric mechanism.

4, gene-amplificative instrament according to claim 1, it is characterized in that: said drive unit comprises a chain gearing and a position control device, in the said chain gearing, one chain is wrapped on two bogie wheels and a driving wheel that are in same horizontal plane, said test tube flat is connected with chain between two bogie wheels, said position control device comprises a guide groove that parallels with horizontal guide rail, the upper and lower plates of guide groove has corresponding through hole on corresponding to the working chamber position, the upper and lower opening of each through hole is provided with a photodiode and a photosensitive receiver pope respectively, said two pipes link with said circuit control section, and a plate washer that is installed on the said test tube flat inserts in the said guide groove.

5, gene-amplificative instrament according to claim 1, it is characterized in that: said drive unit comprises a wire rope gearing, in the said wire rope gearing, one wireline is wrapped on two bogie wheels and a driving wheel that are in same horizontal plane, and the wireline between said test tube flat and two bogie wheels links.

6, gene-amplificative instrament according to claim 1, it is characterized in that: said drive unit comprises a rack and pinion drive, in the said rack and pinion drive, one tooth bar is fixed on the frame, a gear that is meshed with tooth bar is housed on the said test tube flat, and said gear links with the motor that is installed on the test tube flat.

7, gene-amplificative instrament according to claim 1 is characterized in that: said circuit control section is a PC computer controlling machine or a monolithic computer controlling machine.