CN210765338U - Fluorescent quantitative PCR instrument - Google Patents
Fluorescent quantitative PCR instrument Download PDFInfo
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- CN210765338U CN210765338U CN201921549127.3U CN201921549127U CN210765338U CN 210765338 U CN210765338 U CN 210765338U CN 201921549127 U CN201921549127 U CN 201921549127U CN 210765338 U CN210765338 U CN 210765338U
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- quantitative pcr
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Abstract
The utility model discloses a fluorescence quantitative PCR instrument, which comprises a shell, wherein a driving column is vertically arranged in the shell, one end of the driving column is connected with the shell, the other end of the driving column is provided with a feeding disc, a placing hole is arranged on the side wall of the shell close to one end of the feeding disc, a driving device for driving the feeding disc is also arranged in the shell, and a plurality of test tube through holes for placing test tubes are arranged on the feeding disc; a detection device for detecting a sample is arranged in the shell, and a control device for adjusting the position of the detection device is also arranged in the shell; the shell is internally provided with a controller which is electrically connected with the control device and the driving device. This scheme is through the setting of feeding the dish, can transport to the test position detection with placing the test tube that the hole put into, therefore can place a plurality of groups test tube simultaneously on feeding the dish, placing and taking the in-process, need not to stop the detection, raises the efficiency.
Description
Technical Field
The utility model belongs to the technical field of check out test set, especially belong to a fluorescence quantitative PCR appearance.
Background
The principle of the fluorescence quantification is that a specific fluorescent probe is added while a pair of primers is added during PCR amplification, the probe is an oligonucleotide, and two ends of the probe are respectively marked with a report fluorescent group and a quenching fluorescent group. When the probe is complete, the fluorescent signal emitted by the reporter group is absorbed by the quenching group; initially, the probe is bound to any single strand of DNA; during PCR amplification, the probe is digested and degraded by 5 '-3' -exonuclease activity of the enzyme, so that the reporter fluorescent group and the quenching fluorescent group are separated, a fluorescence monitoring system can receive a fluorescence signal, namely, one fluorescent molecule is formed when one DNA chain is amplified, and the fluorescent signal accumulation and the PCR product formation are completely synchronous. Or using the fluorescent dye SYBR. SYBR can be combined on double-stranded DNA, when a template in a system is amplified, SYBR can be effectively combined on a newly synthesized double strand, with the progress of PCR, more and more SYBR dyes are combined, and fluorescence signals detected by an instrument are stronger and stronger, so that the quantitative purpose is achieved, and the quantitative detection device has important significance for molecular biology and medical research.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: provides a fluorescent quantitative PCR instrument which can continuously detect and has no potential safety hazard.
The utility model adopts the technical scheme as follows: a fluorescent quantitative PCR instrument comprises a shell, wherein a driving column is vertically arranged in the shell, one end of the driving column is connected with the shell, the other end of the driving column is provided with a feeding disc, a placing hole is formed in the side wall of the shell, which is close to one end of the feeding disc, a driving device for driving the feeding disc is further arranged in the shell, and a plurality of test tube through holes for placing test tubes are formed in the feeding disc; the shell is internally provided with a detection device for detecting a sample, and the shell is also provided with a control device for adjusting the position of the detection device; the shell is internally provided with a controller which is electrically connected with the control device and the driving device. The setting of drive post and feed plate can rotate under drive arrangement's control, and the setting of test tube through-hole for fixed sample that awaits measuring, detection device's setting is used for detecting the test tube of operation to the assigned position, and detection device up-and-down motion can be controlled in control device's setting, prevents to rotate in-process test tube and detection device at the feed plate and bump, and the controller then is used for controlling the operation of whole device.
Preferably, the control device comprises a control cylinder and a control panel, the control panel is fixedly connected with a piston of the control cylinder, and the detection device is fixedly connected with the control panel. The setting of control cylinder and control panel is convenient for adjust detection device's upper and lower position, and during the in-service use, can set up two control cylinders, can guarantee the stability of detection device operation.
Preferably, the driving device comprises a driving motor and a connecting gear, the driving column is rotatably connected with the shell, a driving gear ring meshed with the connecting gear is arranged on the driving column, and the driving motor is in transmission connection with the connecting gear. The setting of driving motor and connection gear can be exported driving to the drive post with driving motor's rotation to drive and feed the dish and rotate, wherein, the setting of connection gear can adjust the rotational speed through the adjusting gear external diameter, is applicable to not unidimensional sample detection, improves the suitability of device.
Preferably, the detection device is provided with a test tube groove matched with the bottom end of the test tube. The setting of test-tube groove is convenient for adjust the sample temperature, improves intensification and cooling rate to improve detection efficiency.
Preferably, a heat insulation device is further arranged in the shell and comprises a heat insulation cylinder fixedly connected with the shell and a rubber block fixedly connected with a piston of the heat insulation cylinder, the rubber block is matched with an opening of the test tube, the heat insulation device is positioned above the detection device, and the feeding disc is positioned in the middle of the heat insulation device and the detection device. The setting of heat-proof device can prevent that the outside air from getting into in the test tube by the test tube opening in the testing process, causes the influence to the sample temperature, and the setting of block rubber can furthest's cover test tube opening, guarantees the stability of temperature in the testing process.
Preferably, still be provided with cooling fan on the lateral wall of casing, the one end that cooling fan was kept away from to the casing is provided with the air exit. The setting of cooling fan can be cooled down to except that detecting position to outer test tube, avoids the test tube to be in high temperature state all the time, causes the influence to subsequent use, and the mobility of air outside then can improving the casing improves the cooling effect, has got rid of the potential safety hazard (staff scald), and the practicality is strong.
Preferably, the housing is provided with a control panel electrically connected with the controller, and the control panel is located on the outer side wall of the housing. The setting of control panel is convenient for the staff to the control of links such as testing environment, check-out time, sample feed, the operation of being convenient for.
Preferably, an infrared sensor for transmitting signals to the controller is further disposed on the side wall of the housing, and the infrared sensor is located above the detection device. The setting of infrared ray sensor can be when the test tube moves to detection device top, and transmission signal to controller, control drive arrangement close, and the detection device of being convenient for detects, improves the degree of automation of device.
Preferably, the feeding plate on be provided with a plurality of test-tube rack, the test tube on be provided with a plurality of test tube tray, test tube tray and test tube through-hole cooperation setting. The setting of test-tube rack can be taken a plurality of test tubes simultaneously, with the test tube tray that test tube through-hole cooperation set up, can improve the location effect of test tube, guarantees the stability of testing process.
Preferably, the detection device is matched with the test tube on the single test tube rack. With the detection device that test tube cooperation set up on single test-tube rack, can accomplish the detection achievement of test tube on the test-tube rack simultaneously, prevent to appear detecting on the test-tube rack and not detecting the test tube and exist simultaneously, the condition of the operation of not being convenient for.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
1. the utility model discloses in, through the setting of feeding the dish, can transport the test tube of placing the hole and putting into to detecting position and detect, therefore can place a plurality of groups of test tubes simultaneously on feeding the dish, placing and taking the in-process, need not to stop to detect, raise the efficiency, can get rid of the potential safety hazard simultaneously, the practicality is strong.
2. The utility model discloses in, through the setting of rubber piece on testing tube groove and the heat-proof device on the detection device, can guarantee the stability of temperature variation in the testing process, prevent the inspection error because of temperature variation causes.
3. The utility model discloses in, through infrared ray sensor, controller and control panel's setting, can improve the degree of automation of device, be convenient for simultaneously adjust, operate.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic view of the front view cross-sectional structure of the present invention.
Fig. 2 is a schematic side view of the cross-sectional structure of the present invention.
Fig. 3 is a schematic structural diagram of the feeding plate of the present invention.
The labels in the figure are: 1-shell, 2-driving column, 3-feeding disc, 4-placing hole, 5-test tube through hole, 6-detection device, 7-controller, 8-control cylinder, 9-control board, 10-driving motor, 11-connecting gear, 12-driving gear ring, 13-test tube groove, 14-heat insulation cylinder, 15-rubber block, 16-cooling fan, 17-air outlet, 18-control panel, 19-infrared sensor, 20-test tube rack and 21-test tube support block.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention, i.e., the described embodiments are only some, but not all embodiments of the invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiment of the present invention, all other embodiments obtained by the person skilled in the art without creative work belong to the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
As shown in fig. 1, 2 and 3, a fluorescence quantitative PCR instrument comprises a housing 1, a driving column 2 is vertically arranged in the housing 1, one end of the driving column 2 is connected with the housing 1, the other end of the driving column 2 is provided with a feeding tray 3, a placing hole 4 is arranged on the side wall of the housing 1 close to one end of the feeding tray 3, a driving device for driving the feeding tray 3 is further arranged in the housing 1, and a plurality of test tube through holes 5 (eight in this example) for placing test tubes are arranged on the feeding tray 3; the device is characterized in that a detection device 6 for detecting a sample is arranged in the shell 1, and the shell 1 is also provided with a control device for adjusting the position of the detection device 6; and a controller 7 electrically connected with the control device and the driving device is arranged in the shell 1.
Specifically, as shown in fig. 1 and 2, the control device includes a control cylinder 8 and a control plate 9, the control plate 9 is fixedly connected to a piston of the control cylinder 8, and the detection device 6 is fixedly connected to the control plate 9.
Specifically, as shown in fig. 1 and 2, the driving device includes a driving motor 10 and a connecting gear 11, the driving column 2 is rotatably connected to the housing 1, a driving toothed ring 12 engaged with the connecting gear 11 is disposed on the driving column 2, and the driving motor 10 is in transmission connection with the connecting gear 11. .
Specifically, as shown in fig. 2, the detection device 6 is provided with a test tube slot 13 which is matched with the bottom end of the test tube. .
Specifically, as shown in fig. 2, the housing 1 is provided with a heat insulation device, the heat insulation device includes a heat insulation cylinder 14 fixedly connected with the housing 1 and a rubber block 15 fixedly connected with a piston of the heat insulation cylinder 14, the rubber block 15 is matched with an opening of the test tube, the heat insulation device is located above the detection device 6, and the feeding plate 3 is located in the middle of the heat insulation device and the detection device 6.
Specifically, as shown in fig. 1, a cooling fan 16 is further disposed on the side wall of the casing 1, and an air outlet 17 is disposed at one end of the casing 1, which is far away from the cooling fan 16.
Specifically, as shown in fig. 1 and 2, a control panel 18 electrically connected to the controller 7 is disposed on the housing 1, and the control panel 18 is located on an outer side wall of the housing 1.
Specifically, as shown in fig. 2, an infrared sensor 19 for transmitting a signal to the controller 7 is further disposed on a side wall of the housing 1, and the infrared sensor 19 is located above the detecting device 6.
Specifically, as shown in fig. 1 and 2, the feeding tray 3 is provided with a plurality of test tube racks 20 (four in this example), the test tubes are provided with a plurality of test tube support blocks 21, and the test tube support blocks 21 are matched with the test tube through holes 5.
Specifically, as shown in fig. 1 and 2, the detecting device 6 is disposed in cooperation with the test tubes on the single test tube rack 20.
In the using process, test tubes to be detected are placed on the test tube rack 20, the test tube rack 20 is placed into the shell 1 through the placing holes 4, the test tubes are matched with the test tube through holes 5 and placed on the feeding tray 3, at the moment, the other group of test tube racks 20 are positioned right above the detection device 6, the controller 7 starts the control cylinder 8 to drive the detection device 6 to move upwards, the test tubes are matched with the detection device 6 through the test tube grooves 13, the heat insulation cylinder 14 is simultaneously started, the control rubber block 15 moves downwards to cover the opening of the test tubes, then the detection device 6 is controlled to start detection, meanwhile, in the detection process, the cooling fan 16 is always in an opening state, the former group of detected finished products are cooled, after the detection is finished, the controller 7 controls the two cylinders to reset and sends out signals, a worker controls the rotating shaft of the driving motor 10 to rotate through the control panel 18 to drive the feeding tray 3 to rotate by 45 degrees (the, and taking out the detection finished product positioned at the placing opening, placing another group of samples to be detected, and repeating the procedures until all the samples are detected.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. The utility model provides a fluorescence quantitative PCR appearance, includes casing (1), its characterized in that, casing (1) in vertically be provided with drive post (2), the one end and the casing (1) of drive post (2) are connected, the other end of drive post (2) is provided with and feeds dish (3), casing (1) are close to and are provided with on the lateral wall of feeding dish (3) one end and place hole (4), casing (1) in still be provided with the drive arrangement who is used for the drive to feed dish (3), wherein:
the feeding tray (3) is provided with a plurality of test tube through holes (5) for placing test tubes;
a detection device (6) for detecting a sample is arranged in the shell (1), and the shell (1) is also provided with a control device for adjusting the position of the detection device (6);
and a controller (7) electrically connected with the control device, the detection device (6) and the driving device is arranged in the shell (1).
2. The fluorescence quantitative PCR instrument according to claim 1, wherein the control device comprises a control cylinder (8) and a control plate (9), the control plate (9) is fixedly connected with the piston of the control cylinder (8), and the detection device (6) is fixedly connected with the control plate (9).
3. The fluorescence quantitative PCR instrument according to claim 1 or 2, wherein the driving device comprises a driving motor (10) and a connecting gear (11), the driving column (2) is rotatably connected with the housing (1), a driving gear ring (12) meshed with the connecting gear (11) is arranged on the driving column (2), and the driving motor (10) is in transmission connection with the connecting gear (11).
4. The fluorescence quantitative PCR instrument according to claim 1, wherein the detection device (6) is provided with a test tube groove (13) matched with the bottom end of the test tube.
5. The fluorescence quantitative PCR instrument according to claim 1, wherein the housing (1) is further provided with a heat insulation device, the heat insulation device comprises a heat insulation cylinder (14) fixedly connected with the housing (1) and a rubber block (15) fixedly connected with a piston of the heat insulation cylinder (14), the rubber block (15) is matched with an opening of a test tube, the heat insulation device is positioned above the detection device (6), and the feeding plate (3) is positioned between the heat insulation device and the detection device (6).
6. The fluorescence quantitative PCR instrument according to claim 1, wherein a cooling fan (16) is further disposed on the side wall of the housing (1), and an air outlet (17) is disposed at one end of the housing (1) far away from the cooling fan (16).
7. The fluorescence quantitative PCR instrument according to claim 6, wherein the housing (1) is provided with a control panel (18) electrically connected with the controller (7), and the control panel (18) is located on the outer side wall of the housing (1).
8. The fluorescence quantitative PCR instrument according to claim 1, wherein the side wall of the housing (1) is further provided with an infrared sensor (19) for transmitting signals to the controller (7), and the infrared sensor (19) is located above the detection device (6).
9. The fluorescence quantitative PCR instrument according to claim 1, wherein the feeding tray (3) is provided with a plurality of test tube racks (20), the test tubes are provided with a plurality of test tube support blocks (21), and the test tube support blocks (21) are matched with the test tube through holes (5).
10. A fluorescence quantitative PCR instrument according to claim 9, characterized in that said detection means (6) are arranged in cooperation with the test tubes of a single test tube rack (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921549127.3U CN210765338U (en) | 2019-09-18 | 2019-09-18 | Fluorescent quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921549127.3U CN210765338U (en) | 2019-09-18 | 2019-09-18 | Fluorescent quantitative PCR instrument |
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| Publication Number | Publication Date |
|---|---|
| CN210765338U true CN210765338U (en) | 2020-06-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921549127.3U Active CN210765338U (en) | 2019-09-18 | 2019-09-18 | Fluorescent quantitative PCR instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751341A (en) * | 2020-06-24 | 2020-10-09 | 新乡市食品药品检验所 | Traditional Chinese medicinal material component fluorescence analysis detector and analysis detection method |
| CN111925930A (en) * | 2020-08-07 | 2020-11-13 | 单洪瑞 | Virus detector |
-
2019
- 2019-09-18 CN CN201921549127.3U patent/CN210765338U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751341A (en) * | 2020-06-24 | 2020-10-09 | 新乡市食品药品检验所 | Traditional Chinese medicinal material component fluorescence analysis detector and analysis detection method |
| CN111925930A (en) * | 2020-08-07 | 2020-11-13 | 单洪瑞 | Virus detector |
| CN111925930B (en) * | 2020-08-07 | 2023-04-25 | 单洪瑞 | Virus detector |
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